Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025	A→G	E75E (GAA→GAG)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025	0	A	G	92.5%	168.6 / 0.5	68	E75E (GAA→GAG)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base G (28/34);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

GGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGA  >  NC_000913/2783‑3267                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ggAAGTTAGGAGTCTGAAATGGGTAAAGTTTATGCCCCGGCCTCCAGTGCCAATATGAGGGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCGCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTAGCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTaa                                                                                                                                                                                                                                             <  1:37884/251‑1 (MQ=255)       tAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAAt                                                                                                                                                                                                                                       >  1:183510/1‑251 (MQ=255)       tAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAAt                                                                                                                                                                                                                                       >  1:401258/1‑251 (MQ=255)         ggAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGGGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATACTTTATAAGAGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCCAGCAAATTc                                                                                                                                                                                                                                     >  1:323879/1‑251 (MQ=255)                gACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGt                                                                                                                                                                                                                                 <  1:382524/248‑1 (MQ=255)                gACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTg                                                                                                                                                                                                                                <  2:309328/249‑1 (MQ=255)                 aCATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACCCCTGTTGATGGTGCATTGCTCGGAGGTGTAGTCACGGTTGAGGCGGCAGAGGCATTCATTCTCCACAAACTCGGACGATTTGCCGGTAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCCGTGCTGGGAGAGTTTTTGCCAGGAGGTT‑GGACAGCAAATTCCAgggggg                                                                                                                                                                                                                             >  2:591318/1‑246 (MQ=255)                        tAAAGTTTATGCCCCGTCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCTGGGCGGCGGTGACACCTTTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCATTGCTGTGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCatttgctatgact                                                                                                                                                                                                                       <  2:300294/250‑13 (MQ=255)                                   ccctgtcTTCCAGTGCCAATATGAGCGTTGGGTTTGATGGGCTCGGTGAGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCATTCACAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTTCTGGGAGCGTATTTGACAGTAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGTAAAAGaa                                                                                                                                                                                                            <  2:40743/244‑1 (MQ=255)                                    cccGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGAGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAat                                                                                                                                                                                                           >  1:389979/1‑250 (MQ=255)                                         cTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGcc                                                                                                                                                                                                      >  1:185418/1‑250 (MQ=255)                                            ccAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                  <  1:412413/251‑1 (MQ=255)                                            ccAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                  <  1:331953/251‑1 (MQ=255)                                                            cGTCGGGTTTGGTGTGAGCGGGGCGGCGGTGACACCTGTGGATGGTGCATTGCTTGGAGATGTAGTCACGTTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCTGGGAGCGTGTTTGCCAGGAGCTG‑GGCAAGCAAATTCCATTGGCGATGACTCTGGAAAAGAATTTGCCAATCGGTTCGGGCTTAGGCt                                                                                                                                                                                  <  2:34952/251‑1 (MQ=255)                                                                         gtgCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTc                                                                                                                                                                     <  1:538490/251‑1 (MQ=255)                                                                              aggggCAGCGGAGACACATGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCCAAGACATTCCGTCTCCACAACCTCGGACGCTTTGCCGCTAAGCTGCCGTCAGGACCACGGGAAAATCTCGTTTATCAGTGATGGGCGCGGTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATTCATCTGGAAAAGAATATGCCAATAGGTTCGGGCTTAGACTCCAGCGCCTGTTCggagg                                                                                                                                                                >  2:449166/2‑248 (MQ=255)                                                                                        gTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGACAATCGGTTCAGGCTTAGGCTCCAGCGCCTTTTCGGGGGGcgcgtggct                                                                                                                                                      >  2:598968/1‑246 (MQ=255)                                                                                            cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                  >  1:74589/1‑251 (MQ=255)                                                                                            cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                  <  1:180342/251‑1 (MQ=255)                                                                                              ccTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGc                                                                                                                                                <  1:598968/251‑1 (MQ=255)                                                                                               cTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGa                                                                                                                                                                                                                  <  2:187712/184‑1 (MQ=255)                                                                                               cTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGa                                                                                                                                                                                                                  >  1:187712/1‑184 (MQ=255)                                                                                                    gATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑TGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGatgaa                                                                                                                                          <  1:406982/251‑1 (MQ=255)                                                                                                        gggcagtGCTCGGAGATGTAGTCACGGTGGAGGCGGCAGAGTCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCCAGGAGCTT‑GGCAAGCAAATGCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGAGGTCGCGGCGCTGATGGCGAtgaatgaa                                                                                                                                      <  2:73643/245‑1 (MQ=255)                                                                                                          gCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAGTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTACGGTGGTCGCGGCGCTGATGGCGATGAATGAaca                                                                                                                                    <  1:176281/251‑1 (MQ=255)                                                                                                                  cGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGc                                                                                                                             >  1:595577/1‑250 (MQ=255)                                                                                                                   ggagatgacgacACGGTTGAGGCGGCAGAGACATTCAGTCTCAAAAACCTCGGAAGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCATTTATCAGTGCTGGGAGCGTTTTTGCCCGGAGCTT‑GGCAAGCAACTTCCAGTGGCGAGGACTCTGGACAAGAATATGCCAATCGGTTCGGGCTTCGGCTACAGCGCCCGTTCGGTGGTCGCGGGGCTGTGGGGGATGAATGAAAACTgtggaa                                                                                                                            >  2:70200/12‑245 (MQ=255)                                                                                                                        tGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGc                                                                                                                      >  1:498765/1‑251 (MQ=255)                                                                                                                          tAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                    >  1:636959/1‑251 (MQ=255)                                                                                                                          gaGTCACGGTTGAGGCGGCAGAGACATTCAGTCTAACCAACCTCGTAGGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATAGCGTTTATCAGTGCGGGGAGCGTGTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTgtttcaggtcgctt                                                                                                                    <  2:617875/250‑14 (MQ=255)                                                                                                                           aggcACGGTTGAGGCGGCAGAGAAATTCAGGCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCTGCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                    <  2:428810/247‑1 (MQ=255)                                                                                                                           aGTCGCGGTTGAGGCGGCAGAGACCTTCAGTCTCATCAACCTTGGACGCTTTGCCGATAAGCTGCCGTTAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGGTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGAGATGAATGAACACTGTGGCAAGCCGCtt                                                                                                                    <  2:500776/250‑1 (MQ=255)                                                                                                                                cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGac                                                                                                              <  1:647014/251‑1 (MQ=255)                                                                                                                                cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGac                                                                                                              >  2:342319/1‑251 (MQ=255)                                                                                                                                 ggTTGAGGCGGCCGAAACAATTAGACTCAACAACCTCGGACGCTTTGACGATAAGCAGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTATTTGCAAGGAGCTT‑GGCAAGCAAATTACAGTGGCGATGACTATGGAACAGTATATGCCAATCGGTTCGGGCTTAGGCTCCGGCGCCAGTTAGGTGGTCGCGGCGCTGATGGCGCTGAATGAaccgtgtggcaaagcgggtaatgac                                                                                                              >  2:29444/1‑227 (MQ=255)                                                                                                                                 ggTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacc                                                                                                             >  1:453374/1‑250 (MQ=255)                                                                                                                                    tGAGGCGGCAGAGACATTCAGTCGCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACt                                                                                                           <  1:301041/250‑1 (MQ=255)                                                                                                                                                gaCATTCAGTCTCAACAACCGCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCtt                                                                                              <  2:575287/251‑1 (MQ=255)                                                                                                                                                        gTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGCTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACCCTCGTTTGCTGGCTTTGATGGGc                                                                                      >  1:28355/1‑251 (MQ=255)                                                                                                                                                           ccaacaaCCTCGGACGCGTTGCCGAGAAGCTGCCGTCAGAACCACGGGAAAATAGCTTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTG‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGC‑GAg                                                                                  <  2:401258/250‑1 (MQ=255)                                                                                                                                                           acgctctgccgatccGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGTCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCGTTGATGGGC‑GAg                                                                                  <  2:263210/237‑1 (MQ=255)                                                                                                                                                            caacagCCTCGGACGCTTTGCCGATAAGCGGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTGTGCTGGCTTTGATGGGC‑g                                                                                    <  2:74589/248‑1 (MQ=255)                                                                                                                                                              acaacCTCTGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCACGCAAATTCCAGTGGCGATGACTCTGGTAACGAATATGCCAATCGGTTCGGGCTTAGGCTCCCGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCACGCCGCTTAATGACACTCGTTTGCTGGCTTTTAAGGGC‑GATCTg                                                                               >  2:396453/1‑251 (MQ=255)                                                                                                                                                                     cGGACGTTTTGCCGTTAAGCTGCCGTCCGAACCACGGGAAAATATCGTTTAGCAGTTCTGGGAGCGTTTTTGCCAGGA‑GTGTGGCAAGCAAAGTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAGCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggac                                                                        <  2:631281/251‑3 (MQ=255)                                                                                                                                                                          gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAg                                                                                  >  1:263210/1‑236 (MQ=255)                                                                                                                                                                          gCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAggacgaatc                                                                   >  1:21446/1‑244 (MQ=255)                                                                                                                                                                             cacgctcgcccgatccttcaaaaCCACGGGTAAATAGCGTTTATCAGTGCGGGTAGCGTTTTTGCCAGGAGCTT‑GGCAATCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATTACACGCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTcc                                                                <  2:378012/230‑1 (MQ=255)                                                                                                                                                                                 cGATAAGCTGCCGTCAGATCCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGgca                                                            <  1:475998/251‑1 (MQ=255)                                                                                                                                                                                    tAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGGTTAATGCCACTCGTTTTCTGGCTTTTATGGGCCGA‑CTGACAGGACGAATCTCCGgcagcc                                                         >  2:452553/1‑250 (MQ=255)                                                                                                                                                                                      aGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGcatt                                                       >  1:287581/1‑251 (MQ=255)                                                                                                                                                                                             gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAAtctca                                                                >  1:378012/1‑234 (MQ=255)                                                                                                                                                                                             gTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                >  1:46403/1‑251 (MQ=255)                                                                                                                                                                                             gTCAGAACCACGGGAAAATATCGTTTAGCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCGGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACg                                                <  1:618009/251‑1 (MQ=255)                                                                                                                                                                                                    ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                         <  2:636959/251‑1 (MQ=255)                                                                                                                                                                                                    ccACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGt                                         >  1:65022/1‑251 (MQ=255)                                                                                                                                                                                                      aCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATTGGC‑GAGCTGGAAGGACGAATCTCCAGCAGCATTCATTACGACAACGTgg                                       <  1:501287/251‑1 (MQ=255)                                                                                                                                                                                                        gggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCATGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCa                                     <  1:342319/251‑1 (MQ=255)                                                                                                                                                                                                             aaTATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgtg                                <  1:137465/251‑1 (MQ=255)                                                                                                                                                                                                               tatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCCAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCCATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGGACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGCAGGACGAATCTCCGGCAGCCTTCCTTACGACAACGTGGCACCGGGtt                              >  2:381404/1‑251 (MQ=255)                                                                                                                                                                                                                    tttATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAAt                                                                                                                 <  1:675676/164‑1 (MQ=255)                                                                                                                                                                                                                             gcggggAGCGTTTGTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                <  2:287581/248‑1 (MQ=255)                                                                                                                                                                                                                             gCTGGGATCGTTTTTGCCCGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGTGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATg                <  2:21446/251‑1 (MQ=255)                                                                                                                                                                                                                             gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAACAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                            >  2:76827/1‑160 (MQ=255)                                                                                                                                                                                                                             gCTGGGAGCGTTTTTGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                            <  1:76827/160‑1 (MQ=255)                                                                                                                                                                                                                                      gTTTTTGACAGGAACTT‑GGCAAGCAAATTCCCGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGGTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGAAGAATCTCCGGCAGCATTCATTACGACAGCGTGACACCTGGTTTTCTTGGTGGAATGCAGTtgatg       >  2:615022/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttttGCCAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtgatga      <  1:591318/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccAGGAGCTT‑GGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGATGGCTTTGATGGGC‑GAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga  >  1:434613/1‑249 (MQ=255)                                                                                                                                                                                                                                             ccAGGAACTT‑GGAAAGCAACTTCCAGTGGCGATGACTCTGGTAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGCATGAACAATGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTTGCAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCTTGTTTTCTTGGAGGTATGCAGTGTATGATCga  >  2:248952/1‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTG‑GGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGC‑GAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGA  >  NC_000913/2783‑3267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑