Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	774	T→C	T146T (ACT→ACC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	774	0	T	C	100.0%	124.7 / NA	38	T146T (ACT→ACC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/5);  total (33/5)

CAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTT  >  NC_000913/528‑1003                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      cAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGtt                                                                                                                                                                                                                                    >  1:617819/1‑250 (MQ=255)  agctaTGCCGAACGTATTTATGCCGACCTTGTGACACACCTCGTCGCCGGCCAGCCGGAATTCCCGCTGGCGCAATTGAAAACTTCCGTCGATCATGAATTTTCCCAAAGAAAACATGTCCTCCATGGCACTAGTTTGTTGGGGCAGTGCCCGGATATCATCAACGCTGCGCTGATTTGCCGTGGCTATAAAATTTCGATCTCCATTCTGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTAt                                                                                                                                                                                                                                  <  1:51269/247‑1 (MQ=255)         ccGAACGTATTTTTGCCGAACTTTTGACGAGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCg                                                                                                                                                                                                                           >  1:657323/1‑251 (MQ=255)          cGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCgg                                                                                                                                                                                                                          >  1:515278/1‑251 (MQ=255)               gTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCCTTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGa                                                                                                                                                                                                                      >  1:467736/1‑250 (MQ=255)                tATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGaa                                                                                                                                                                                                                     >  1:613826/1‑250 (MQ=255)                  tttttGACGAACTTTTGGCGGGACTAGCCGCCGCCCAGCCGGGCTTCCCGCTGGCGCAATTGAAAACTTTCGTAGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGAAGTGCCCGGCTAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGCTCGCCATTATGGCCGACGTATTAGACGCGGGAGGTCACACCGATACCGTTATCGCTCCgggcgcaaa                                                                                                                                                                                                                   >  2:1209/1‑243 (MQ=255)                        ccGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAActgctg                                                                                                                                                                                                            >  1:239395/1‑251 (MQ=255)                                  taCGGGACGCGCCGCCGCCCAGCCGGGAGTCCCGCGGGCTCAATTGAAAACTTGCGTCGAGCAGGAATTTGCCCAAATAAAACAGGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGAGAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTgggg                                                                                                                                                                                                   <  2:355054/249‑1 (MQ=255)                                       gACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTAc                                                                                                                                                                                             >  1:123437/1‑251 (MQ=255)                                           cgccgccgcCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCg                                                                                                                                                                                         <  1:151073/251‑1 (MQ=255)                                                                      ggCGCAATTGAAAACGTTCGTCTATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTGGGGGCAGTGCCCGGATAGCATCAACGCTTCGCGGATTTGCCGTGGCGAGAAAATGTCGATCGCTATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCTTTATCGATCCGGGCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCGACCGTCGATATTGCTGAGTcc                                                                                                                                                               <  2:613826/250‑1 (MQ=255)                                                                                           cgATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATcc                                                                                                                                                                                                                            >  1:189136/1‑168 (MQ=255)                                                                                            gATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGGATTACCTCGAATCTACCGTCGATATTGCTGAGTCAACCCGCCGTATTGCGGCAAgtcc                                                                                                                                        >  2:360343/1‑248 (MQ=255)                                                                                                    aTTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATACGGTCGAAAAACTGCTGGCAGAGTGGCATTACCGCGAATCTACCGTCGATATTGCTGAGTCCCCCCGCCGTATTGAGGCCAGTGGCATTCCgg                                                                                                                                >  2:325789/1‑251 (MQ=255)                                                                                                        gCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTTCGGCAAGTCGCATTCCGGCtt                                                                                                                             >  2:78575/1‑249 (MQ=255)                                                                                                         cccAAATAAACCATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTCTTAGAAGCGCGCGGTCAAAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGGTATTGCTGAGTCCACCCGCCGTATTTGGGCAAGTCGCATTCCGGCTGAt                                                                                                                           >  2:83639/1‑251 (MQ=255)                                                                                                                         ccTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGCATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATgg                                                                                                           >  2:214416/1‑251 (MQ=255)                                                                                                                          cTGCATGGCATTAGTTTGTTGGGGCCGTGCCCGGATAGCATCACCGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCAATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTTCCGTTAACGATCCGGTCGAAACACGGCTGGCAGTGGGGCCTTTCCACGAATCTACCGTCGATTATGCTGAATCCCCCCGCCGAATTGCGGCAAGTCGCATTCCGACTGGTCACATGGGACTGATgg                                                                                                           >  2:398358/1‑250 (MQ=255)                                                                                                                                                ggCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGa                                                                                     >  1:549299/1‑250 (MQ=255)                                                                                                                                                        ccGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCt                                                                                                                              >  1:513121/1‑201 (MQ=255)                                                                                                                                                        ccGGATAGCATAAACGCTGCGCTGAATTGCCGTGGCGAGAAAATGTCGAGCGCAATACTGGCCGGCGTATTAGAAGAGCGCGGTCTCAACGTTCCCGTTATCGATCCGGTCGAAAGACTGCTGGCGGTGGGGCATTGCCGCGACTCTACCGTCGATATTGCTGAGTCCACCCGACGTATTTCGGCAAGTCGCTTTCCGGCTGTTCACATGGGGATGGTGGCCGGTTTAACCGCCGGTAATGAAAAAGGCgg                                                                            >  2:233340/1‑250 (MQ=255)                                                                                                                                                         cGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGa                                                                            >  1:215441/1‑250 (MQ=255)                                                                                                                                                                  tCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAACGGCGGACTggtggt                                                                   >  1:676358/1‑250 (MQ=255)                                                                                                                                                                      cgctgcgcggATTTGCCGTGGCGAGAAAATGTCGCTCGACATTATGGCCGGCGGATTAGAAGCGCGCGGTCTCGACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCAGTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCACGTCGCATTCAGGCTTGTCACATGGAGCTGAAGGCAGGTTTACCCCCCGGTAGTGAAAAAgaagaaccggggggaattg                                                              >  2:494519/1‑233 (MQ=255)                                                                                                                                                                           cgctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGATATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCCTTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGATGGTGGCAGGTTTCACCCCCGGTAATGAAAAAGGCGAACTGGGGGAACTTGGACGc                                                         >  2:128778/1‑251 (MQ=255)                                                                                                                                                                             ctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCa                                                        >  1:411671/1‑250 (MQ=255)                                                                                                                                                                               gATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGACTTGGACGCaccg                                                     >  1:524860/1‑248 (MQ=255)                                                                                                                                                                                           cGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCCACGGTTCCGacacct                                         >  2:669351/1‑247 (MQ=255)                                                                                                                                                                                                  aTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTTCCTCGAATCTa                                                                                                                                                                                   <  2:339621/106‑1 (MQ=255)                                                                                                                                                                                                  aTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTa                                                                                                                                                                                   >  1:339621/1‑106 (MQ=255)                                                                                                                                                                                                  aTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACAGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGCCGCAACGGTTCCGACTActccgcggc                                  >  1:43059/1‑245 (MQ=255)                                                                                                                                                                                                                tATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGGGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGGAATGAAAAAGGCGAACTGGCGGGACTTGGGCGCAACGGTTCCGACTActccgaggcggggggtggtgacc                    >  2:581259/1‑231 (MQ=255)                                                                                                                                                                                                                    gCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGAGGTGGCTGCCTGttt                >  2:441901/1‑251 (MQ=255)                                                                                                                                                                                                                       ggCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTa               >  1:428024/1‑249 (MQ=255)                                                                                                                                                                                                                         cGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACtg                                                                        >  1:644604/1‑190 (MQ=255)                                                                                                                                                                                                                            aTTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGGCGCCTGTTTTCGCGccc        >  2:94603/1‑250 (MQ=255)                                                                                                                                                                                                                                  aGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCGTTTTACGCCCCGgttgtt  >  2:88358/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      CAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTT  >  NC_000913/528‑1003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑