Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,663	C→T	S533F (TCT→TTT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,663	0	C	T	100.0%	134.9 / NA	44	S533F (TCT→TTT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/17);  total (27/17)

CAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTG  >  NC_000913/1252429‑1252908                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      cAATTGATGCAAAAGTGTTTGCAACAACTGCCGCGGCCCTCGCAATGCTGGTGGAAAAAAACCTTTTTATTCGCCAG‑‑TGTTATTACGCGTTGCATGAATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGc                                                                                                                                                                                                                                       <  1:372080/251‑1 (MQ=255)           cAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCACGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCATGCAACGATTTTg                                                                                                                                                                                                                             >  1:270115/1‑251 (MQ=255)                    tGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTAGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGACGGCTTGAAAAACGCTTCTCTACGCGGCTGACAATTGATTACGATGCCCTCACGCGCCTGGTTTTTTGTGCATGGCCAGGAAACGATTTTGCACtttccc                                                                                                                                                                                                                    >  2:161486/1‑248 (MQ=255)                       aacaacGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCg                                                                                                                                                                                                                 >  1:298507/1‑251 (MQ=255)                                  cgGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGGTTTTGAACTTTACAGCGTCCTCGCGAAt                                                                                                                                                                                                      >  2:161505/1‑251 (MQ=255)                                   ggACCTCGCAATGCGGGGGGAACAAATCCGTTTTAGTC‑CCAGGCTGT‑ATTACGCGCTGAATGCATTTAAAATTTCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATc                                                                                                                                                                                                     <  1:161486/251‑1 (MQ=255)                                             aTGCTGGTGGAACACAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATACCGGGTCTGGTG‑AATAACAAATTAGGCAGTCTTGAGAAACGATTTTCTACGGGGCTGAAAATTGATGACGATGCAATCGGTAGCCTGGTTTTTTGTGCATGGATAGGAAACGATTTTGAATTTTACAgagtaatggagtatattgcgctg                                                                                                                                                                                             >  2:277047/1‑227 (MQ=255)                                             aTGCTGATGGAACAAAATCGTTTTAGTCACCAG‑CTGT‑ATTAAGCGCTGCATGCATTTGAAATTACCATCACGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCCGGAG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGCTGCCCTCGCTCGCCTGGTTTTTTGTTCCTGGCAAGGTAACGATTTTGAACTTTACAGAGTCATCGAAAATCTTGatctt                                                                                                                                                                                             >  2:395166/1‑248 (MQ=255)                                                   gtggAACAAAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTg                                                                                                                                                                                      >  1:481692/1‑250 (MQ=255)                                                        aCAAAACCGTTTTAGTCGCCAG‑CGTT‑ATTACGCGCTGATTTCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACg                                                                                                                                                                                <  1:124719/251‑1 (MQ=255)                                                                            aG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCa                                                                                                                                                                          >  1:105627/1‑237 (MQ=255)                                                                              agttgttttACGCGCAGCATGCATTTGAAATTACCACTCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCa                                                                                                                                                                          <  2:105627/230‑1 (MQ=255)                                                                                 gT‑ATTACGCGCTGCATGCATTTGAAATTCCCACCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTg                                                                                                                                                         <  1:161505/250‑1 (MQ=255)                                                                                     ttgcgcgtgtcatgcatgTGAATTTCCCATCCCGCCTCTGCGGATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCTCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACGTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCg                                                                                                                                                      <  2:386420/240‑1 (MQ=255)                                                                                       aCGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGa                                                                                                                                                    >  1:448636/1‑250 (MQ=255)                                                                                        cgcgCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAAc                                                                                                                                                  >  1:268360/1‑251 (MQ=255)                                                                                              gaggtgttgtgtaaacccctgcccGCCTCTGTGTATTCGGCCTGTCAGCCATCCGGCGCTTGTG‑AATAACAAATTACGCAGGCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGACCTCGCTCGCCTGGTTTTTTGTGCATGGCCGGGCAATGATTTTGAACTTTGCAGCTGCATCGTGAATCGTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCAGGACCACCTGt                                                                                                                                            <  2:114816/230‑1 (MQ=255)                                                                                                atgcatTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAACAGCTCCTCTACGCGGCTGAAACTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCCTGGCCAGGCAACGTTTTTGAAATTTACAGCGTCATCGAGACTCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTACTTTTCCGGAACATCTGttt                                                                                                                                          >  1:651796/1‑251 (MQ=255)                                                                                                        gAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAga                                                                                                                                   >  2:620524/1‑249 (MQ=255)                                                                                                                    ccGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAgatg                                                                                                                       >  1:409625/1‑250 (MQ=255)                                                                                                                      gCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGCCAgctgctg                                                                                                                    >  2:674807/1‑245 (MQ=255)                                                                                                                             cGTATGCGGCGTTGCAGCATTCCGGCGATGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATTCCCTCGCTCGCCTGGTCTTTTGGGCATGGCAAGGCAACGATTTTGAGCGTTACAGCGGCATCGAGAATCTTGTTCTGAGTAGTGTTAACGCGCGCATTCCCGTCAGTGATTTGCCGGAACGTCTGTTTACCgggtaggcatcagaggtggtaaggcc                                                                                                             >  2:1435/1‑226 (MQ=255)                                                                                                                                       gTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCtt                                                                                                   <  1:59806/251‑1 (MQ=255)                                                                                                                                       gTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCGTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGGGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCtt                                                                                                   <  2:309141/251‑1 (MQ=255)                                                                                                                                        gggCAGCCTTCCGGCG‑TGGTGGAATAAAAAATTCCGCATTCTTGAAAACCGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCGGGTTTTTTGTGCATGGCCGGTCACCGATTTTGAACTTTACAGCTTCATCGAGATTCTGGCTCGGAGTAGTGACAACGGGCGCATGCGCGCCAGTGATTTGCCGGAACATCTGTTTCCCGAGCAGGCGCCAGAGGATGTGAGCGCCACCCGCCttt                                                                                                  <  2:171143/250‑1 (MQ=255)                                                                                                                                         ggCAGCATTCCGGCGCTGGTG‑AATACCACATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTc                                                                                                 >  1:544052/1‑251 (MQ=255)                                                                                                                                          gcagcaTTCCGGCGCTGGTG‑AATAACAAATTACGCCGTCTTGAAAACCGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGACCAGACGACAGATGATGTCAGCCCCACCCGCCTTTcc                                                                                                >  2:162566/1‑251 (MQ=255)                                                                                                                                                       gcTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATTACGAAGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTTAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAt                                                                                   <  1:618235/251‑1 (MQ=255)                                                                                                                                                          ggTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAACATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATGACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGGCAGATGATGTCAGCGCCCCCCGCCTTTCCACCAGTCTGTCATTTg                                                                                >  2:278548/1‑251 (MQ=255)                                                                                                                                                          ggTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCCACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAttt                                                                                 <  1:1435/250‑1 (MQ=255)                                                                                                                                                                   aCAAAATACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGCTGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGATTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCCGTATGTCCTTTGCGGACGtt                                                                        >  2:100799/1‑251 (MQ=255)                                                                                                                                                                        ttACGCAGTCTTGAAAAACGCTTCTCTCCGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGCTTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATACAGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCTGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGaaa                                                                    >  2:13838/1‑250 (MQ=255)                                                                                                                                                                             cAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAgg                                                              >  1:169003/1‑251 (MQ=255)                                                                                                                                                                                                    cgcgGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTc                                       <  1:674807/251‑1 (MQ=255)                                                                                                                                                                                                            aaaaTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCgg                               >  1:127432/1‑251 (MQ=255)                                                                                                                                                                                                             aaaTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGt                              <  1:469518/251‑1 (MQ=255)                                                                                                                                                                                                              aaTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGtg                             >  1:127003/1‑250 (MQ=255)                                                                                                                                                                                                               aTTGATGACGATGCTGTCGCTCGCCTGGTTTTTTGTGCATGGTCGGGAAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCACTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGGCAGCGCCACCCGCCTTTCCCCCAGGCTGTCAt                                                                                   <  2:222689/196‑1 (MQ=255)                                                                                                                                                                                                               aTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCCTGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTATTTACCGAGCAGGCGACAGATGATGTCCGCGCCACCCGCCTTTCCACCAGTCTGTCAt                                                                                   >  1:222689/1‑196 (MQ=255)                                                                                                                                                                                                                  gatgaCGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCAt                         >  1:74495/1‑251 (MQ=255)                                                                                                                                                                                                                       cGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGACGCAATCAgg                    >  1:451327/1‑251 (MQ=255)                                                                                                                                                                                                                         agtcccgcgctcccCTGGTTGTTTGTGCAGGGCCAGGCCACGATTATGAACTTTACAGGGTCATTCAGAATCTTTCTCTGAGTATGGATAACGGGCGCATTCCCGTCAGTGATTTGCCGGAACATCTGTTTCCCGAGCAGGCGACAGATGATGTCAGCGCCACCCTCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACATGCGGTCGCATTCAGGaa                  <  2:544052/244‑1 (MQ=255)                                                                                                                                                                                                                               tcgctcgcCTGGTTTTTTGTGCATGGCCAGGCCACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCg            >  1:253855/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGATAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTg  <  2:127432/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG‑CTGT‑ATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTG‑AATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTG  >  NC_000913/1252429‑1252908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑