Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,244,908	C→T	L61L (CTG→TTG)	ymgE →	PF04226 family protein YmgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,244,908	0	C	T	100.0%	85.8 / NA	29	L61L (CTG→TTG)	ymgE	PF04226 family protein YmgE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/25);  total (4/25)

TAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTT  >  NC_000913/1244672‑1245153                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      tAGAGCGTTGATGTAAGTATTACTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTGTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGTGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCt                                                                                                                                                                                                                                          <  2:4859/250‑1 (MQ=255)                              ccGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCt                                                                                                                                                                                                            <  1:424789/251‑1 (MQ=255)                                   tCATCGAAACGAGGGCCTGAACATGGGACTTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGGGGCGGGGGGGGGAGGTATTGTCGTTCTGGGcc                                                                                                                                                                                                       >  1:223149/1‑250 (MQ=255)                                                gtccTGAACATGGTAATTATTGCCTGGATTATTTTTGACCTGAGAGCCGGCAGTATCGCCAAGCTAAGCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCGCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTAGTCTCGTTCTGGGCATATTCCGcctcct                                                                                                                                                                                          <  2:279504/249‑1 (MQ=255)                                                 gCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTg                                                                                                                                                                                         <  1:162263/251‑1 (MQ=255)                                                               attattGCCTGGATTATTTTTGACCTGATAGCCGGCATTAGTGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTGCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGa                                                                                                                                                                           <  1:464726/251‑1 (MQ=255)                                                                           attattTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGc                                                                                                                                                               <  1:89171/251‑1 (MQ=255)                                                                           attattTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGc                                                                                                                                                               <  1:398805/251‑1 (MQ=255)                                                                            tttttgttGACCTGGTAGCCGGCATTAACGCCAAGCTAAACATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCGGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTTGCATTGGCGGCTCCATCAGTGGTTTTAAGTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGc                                                                                                                                                               <  2:66852/244‑1 (MQ=255)                                                                                tttttacctgagtgctggtcttaacGCCAAGCTAATCATGCCTGTGCGTGATGGTGTTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCTGTGCGTTGGGCGGCGGCTGTCTGGCGACCATGTTTGGCAGGGGCGGCGCCACCAGTGGTGTTAATTTGCACAGCTTCCTTGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCTTCCTGCGAAGAGAATAAGAGTTTCACAAGGCGGAt                                                                                                                                                           <  2:290278/226‑1 (MQ=255)                                                                                   tGACCTGATAGCCGGCATTATCGCCAAGATAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGTCCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGc                                                                                                                                                        <  1:76850/250‑1 (MQ=255)                                                                                     aCCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGAt                                                                                                                                                     <  1:387980/251‑1 (MQ=255)                                                                                         gATAGCCGGCATTATCGCCAAGCTAATCAGGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTGTTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTTGCGACCATGTTTGGCATTGGCTGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTTGTGGGAGCTATTCGCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATAc                                                                                                                                                   <  1:124483/249‑1 (MQ=255)                                                                                          aTAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGa                                                                                                                                                <  1:11705/251‑1 (MQ=255)                                                                                                      aTCGCCAATATAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTAGTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCg                                                                                                                                    <  1:415643/251‑1 (MQ=255)                                                                                                      aTCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATcc                                                                                                                                     <  1:227324/250‑1 (MQ=255)                                                                                                                         cGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGtat                                                                                                                 <  1:15763/251‑1 (MQ=255)                                                                                                                          ggggCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGGGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAAATg                                                                                                                                              >  1:96039/1‑221 (MQ=255)                                                                                                                          ggggCGTGATGGTGGTGGAGTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATGGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATg                                                                                                                                              <  2:96039/221‑1 (MQ=255)                                                                                                                                                                    gatattgggtgcgttgtcacgtggctggatTGCGAGCATGGTTGGCATTGGCTGCTCCTGCACTTGTTTTAATTTGCACAGCTTCCCGGTGGCGGTGGTGGGAGCTATTCTAGTTCTGGGCATATTCCGCCTCCTGCGAAGAGACTAAGATTTTCATAAGGCGGTTAGTTTTACAGATGCCGCGATCCGCTTTCTCATCAGATCGTATACTCTACGCCTGCTTTACGGGTGAAAAAAATCAATATAGctct                                                                      <  2:335663/229‑4 (MQ=255)                                                                                                                                                                          cggtgcggtgGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGcc                                                                                                                                            <  1:586369/175‑1 (MQ=255)                                                                                                                                                                            gtgcggtgGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAg                                                                                                                        <  1:368227/193‑1 (MQ=255)                                                                                                                                                                                                 tggcGACCATGTTTGGCATTGGCGGCTACATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAAGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCggg                                          >  1:129674/1‑249 (MQ=255)                                                                                                                                                                                                       ccATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTaacaaca                                   <  1:274912/251‑1 (MQ=255)                                                                                                                                                                                                                    tGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCtttt                      >  1:154919/1‑251 (MQ=255)                                                                                                                                                                                                                        ggcTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGGGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTaa                  <  1:444844/251‑1 (MQ=255)                                                                                                                                                                                                                                 agttgTTTTTATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATGCTCGTTCTTGGCATATTTCGCCTCCTGCGAAGAGAATAATATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACGTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGttt         <  1:433791/247‑1 (MQ=255)                                                                                                                                                                                                                                     gTTTTAATTTGCACAGCTTCCTGGTGGCGTTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCgtt     <  2:166727/251‑1 (MQ=255)                                                                                                                                                                                                                                         tAATTTGCACAGCTTCCTTGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCgttgtt  <  1:391937/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      TAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTT  >  NC_000913/1244672‑1245153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑