Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,180,126	G→A	A203T (GCG→ACG)  A203T (GCG→ACG)	cobB → cobB →	protein‑lysine deacetylase/desuccinylase/lipoamidase protein‑lysine deacetylase/desuccinylase/lipoamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,180,126	0	G	A	95.5%	118.2 / NA	45	A203T (GCG→ACG)  A203T (GCG→ACG)	cobB cobB	protein‑lysine deacetylase/desuccinylase/lipoamidase protein‑lysine deacetylase/desuccinylase/lipoamidase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (19/23);  minor base C (1/1);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGAT  >  NC_000913/1179879‑1180360                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            cGGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGt                                                                                                                                                                                                                                          >  2:454291/1‑251 (MQ=255)           tttttGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGc                                                                                                                                                                                                                                >  1:123909/1‑251 (MQ=255)                    gggACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTTTCGATGGCCGATAtttt                                                                                                                                                                                                                       <  2:386224/249‑1 (MQ=255)                             aaTATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCTTATGCATGGGGAACTGCTGAAAGTGTGTTTTTCACAAAGTGGTCAGGTTCTCTACGGGACAGGAGACGTTACCCCAGAATATAAATGCCATTGTTGCCAGTTTTCGGCACCCTTGCGCCCGCACTTAGTGTGGTTTGGCGAAATGCCTCTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGAGATTTTCATTGCCAt                                                                                                                                                                                                              <  2:607180/251‑1 (MQ=255)                                             aTGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCAt                                                                                                                                                                                              <  2:372184/251‑1 (MQ=255)                                             aTGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCAGGGCAt                                                                                                                                                                                              >  2:204481/1‑251 (MQ=255)                                                      cAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCAGTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCcg                                                                                                                                                                                     <  2:137905/251‑1 (MQ=255)                                                         gTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCcggcg                                                                                                                                                                                  <  2:494025/251‑1 (MQ=255)                                                                          cATATGCCTGGGGAACTGCTGAAAGTGAGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATcc                                                                                                                                                                                      >  2:252543/1‑230 (MQ=255)                                                                          cATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATcc                                                                                                                                                                                      <  1:252543/230‑1 (MQ=255)                                                                            tatGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTTCCATTGGCCCATCCGGGCATGTTAACACGGCGGCGGGGTTTGttaccga                                                                                                                                                                 >  2:471067/1‑244 (MQ=255)                                                                                       aaCTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCCTTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCAt                                                                                                                                                    >  2:503449/1‑251 (MQ=255)                                                                                           gctgAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGgcg                                                                                                                                                >  1:478553/1‑251 (MQ=255)                                                                                           gctgAAAGTGCAATGGTCACATAGGGGTCAGGCTCTCAACTGGACAGGAGCAGTAACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGTCACCCATGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGAGGACATTTATCTGACGTTGTCGATGGCTGATATTTGCATTGCCATTGGCACATCAGGGCATGGTTATCCGGCGTCTGGTTTAGTTCACGCAGCGGAAATGCGTGgcg                                                                                                                                                >  2:204828/1‑251 (MQ=255)                                                                                              gAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGgcgcgc                                                                                                                                             <  1:149480/251‑1 (MQ=255)                                                                                                      gttgttCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTg                                                                                                                                     <  1:179814/251‑1 (MQ=255)                                                                                                           tCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAAc                                                                                                                                 <  1:177493/250‑1 (MQ=255)                                                                                                                   tGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTg                                                                                                                        >  1:616234/1‑251 (MQ=255)                                                                                                                         ggTTCTCGACTGGACAGGAGACGTTACCCCAGACGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTg                                                                                                                        >  2:235037/1‑245 (MQ=255)                                                                                                                         ggTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTg                                                                                                                        <  1:235037/245‑1 (MQ=255)                                                                                                                          gtgttcGACTGGACAGGGGACGTGAACCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAg                                                                                                                 <  2:137239/247‑1 (MQ=255)                                                                                                                             ctcGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGTTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGGGTc                                                                                                               >  1:474153/1‑250 (MQ=255)                                                                                                                                gACTGGACAGGAGACGTTACCACAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACACTTACGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCTCATCCGGCCATGTTTATTCGGCTGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAAACGAGTCCGGt                                                                                                           >  2:406694/1‑251 (MQ=255)                                                                                                                                       cAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAAt                                                                                                    >  1:653604/1‑251 (MQ=255)                                                                                                                                        aGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATg                                                                                                   <  2:505541/251‑1 (MQ=255)                                                                                                                                                     ccAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGCTGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGGTTGTTCACGAAGCGAAAATGCATGGCGCGCACACCGTGGAACTGAATATTGAACCGAGTCAGGTTGGTAATGGATTTGCCgaga                                                                                       >  2:508102/1‑250 (MQ=255)                                                                                                                                                         aagaTAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAatat                                                                                  <  1:503449/251‑1 (MQ=255)                                                                                                                                                           gaTAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGATAATGAATTTGCCGAGAAATATTa                                                                                <  1:188070/251‑1 (MQ=255)                                                                                                                                                           gaTAAATGCCATTGTGGCCAGTTTCCGGCACCCTTGCGCCCGCACGTGATGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTGCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATGTGCCGAGAAATATTa                                                                                <  2:616234/251‑1 (MQ=255)                                                                                                                                                                aTGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGcc                                                                           <  1:338312/251‑1 (MQ=255)                                                                                                                                                                        gtagccaggtaccgtgaCCCGTGCGCCCGCACGTAGTGTTGTTGGGCGAAATGCCACTCGTCATGGATGAAATTTATATGACGGTGTCGATGGCCGATATTTTCATTGCCATGGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAATCGAATCTGCATGGCTCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCACGG‑CAAga                                                                  <  2:208079/235‑2 (MQ=255)                                                                                                                                                                                  tCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGgtggtg                                                         <  1:474127/250‑1 (MQ=255)                                                                                                                                                                                    cGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCt                                                      >  1:339269/1‑251 (MQ=255)                                                                                                                                                                                                                gTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGAt                          <  1:590415/251‑1 (MQ=255)                                                                                                                                                                                                                       gAAAAGCCACTCGGCATGGATGAAATTTAGATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACCTCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTTACGAAGCGAAACTGCATGTCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTAAGGCCCGG‑CAAACCAGGTGGTGCCTGGGGTTGTTGTAAAATTgattcgggggttaaaa                     >  2:372796/1‑234 (MQ=255)                                                                                                                                                                                                                             ccACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAg             <  1:600441/251‑1 (MQ=255)                                                                                                                                                                                                                              cACTCGGCATGGCTGAAATTTATATGCCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGACCCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAACCAGGTGGTGCCTGGGTTTTTTGGAAAGTTTCTTACGGGATTAAAAGCGGGGAGc            >  2:546416/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGcc      <  1:231739/251‑1 (MQ=255)                                                                                                                                                                                                                                     cATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGATTCTTGACCCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGACAA‑CAAGGTGGTGGTTGAGTTTGTTGAGAAGTTGTTGAAGGGATTAAAAGcagtacgaattctct     >  2:46658/1‑237 (MQ=255)                                                                                                                                                                                                                                     cATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCt     <  1:37683/251‑1 (MQ=255)                                                                                                                                                                                                                                       tGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGCGTCAGGTTGGTAATGAATTTGCCGAGGAATATTACGGCCCGG‑CAAGCCCGGGGGGGCCTGAGTTTTTTGAAAAGTTTCTGGAGGGATTTAAAGCGGGAAgacatgcctgt   >  2:640231/1‑241 (MQ=255)                                                                                                                                                                                                                                       tGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTa                                                                                                      >  1:72450/1‑153 (MQ=255)                                                                                                                                                                                                                                        ggATGAAATTTATATGCCGTTGTCGATGGCCGATATGTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGAt  <  2:123909/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGAt  <  1:471067/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAACCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCgac                                                                       >  1:273579/1‑176 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CGGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGG‑CAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGAT  >  NC_000913/1179879‑1180360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑