Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,161,403	G→A	A50V (GCC→GTC)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,161,403	0	G	A	100.0%	172.3 / NA	56	A50V (GCC→GTC)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/35);  total (21/35)

TGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATG  >  NC_000913/1161175‑1161645                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    tGGAATCCGCTGTAATAATAAAGAGCTCTATCGGAATCCGCCTGCCTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCTGCGTTTGTAACTGCTGATCTTCCAGCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAAGCACCg                                                                                                                                                                                                                                <  2:511243/250‑1 (MQ=255)  ggAATCCGCGGGAAGAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGtt                                                                                                                                                                                                                              <  1:636120/251‑1 (MQ=255)                 aataaAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTggg                                                                                                                                                                                                               >  2:195428/1‑250 (MQ=255)                           aCGATCGGAATCCGCCTGACTTTTGCTGCTCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCTCGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACTATCTCCGTTTCTTCAGTGGCTGGTGCAGCAAAAg                                                                                                                                                                                                     <  1:272807/251‑1 (MQ=255)                                      ccGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTAAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAgg                                                                                                                                                                                           <  2:547225/250‑1 (MQ=255)                                       cGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGt                                                                                                                                                                                          >  1:109465/1‑250 (MQ=255)                                             aCTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTg                                                                                                                                                                                   <  1:153916/251‑1 (MQ=255)                                             aCTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGAAATAGTg                                                                                                                                                                                   >  1:502744/1‑251 (MQ=255)                                                       aTCCCCAGAGTGTTTTCCATCACTTAGACCAGCGTTTGTACATACTGATCTTCCATCCGCTGCTGGCTGCCAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCAGCAGGGGTAGACGTTACGCTGTAATCATTTTCGCAATCATCTGGCGCTGTGACTGACCTCTCAACAATCAACGTTTATTCAGTAGCTGGTGCCGCAAAAGCgggaacaggaaatagagccacaggagt                                                                                                                                                                         >  2:349536/1‑226 (MQ=255)                                                              gCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCg                                                                                                                                                                  <  1:159192/251‑1 (MQ=255)                                                                     ttCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagt                                                                                                                                                            >  1:154989/1‑250 (MQ=255)                                                                      tccatccctctgCCCAGCGTTTGTAACTGCTGATCTTCCATCCTCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagta                                                                                                                                                           <  2:239072/239‑1 (MQ=255)                                                                           cACTTCGCCCAGCGTTTTTAACTGCTGATCTCCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGAc                                                                                                                                                      <  2:204307/250‑1 (MQ=255)                                                                            aCTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACgg                                                                                                                                                    >  1:60445/1‑251 (MQ=255)                                                                             cTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCCACCGGCAAGTAGAGACggg                                                                                                                                                   >  1:414133/1‑250 (MQ=255)                                                                                   ccAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTg                                                                                                                                            <  1:659838/251‑1 (MQ=255)                                                                                    cAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGGAGGGAATAGTGCCCGTGCTATGCCACCGGCAAGTAGTGACGG‑TTTggggg                                                                                                                                           >  1:42213/1‑248 (MQ=255)                                                                                       cGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCCTTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCGGCAAAAGCGGCAGAAAGATATAGTGCCAGTGCTATGCACACGGCAAGTAGTAACGGGTTTggggggg                                                                                                                                         >  2:207361/1‑246 (MQ=255)                                                                                       cGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATgg                                                                                                                                        <  1:135961/251‑1 (MQ=255)                                                                                          ttGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCt                                                                                                                                      <  1:357876/250‑1 (MQ=255)                                                                                              aacggcttaTCTTTCATCCGCTGCTGGCTAAAAATAGGGACCGAC‑TGAGGAATAGCGCGTGGAGGTCA‑CTGCAGTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCCGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCGATGCAACCGGCAAGTAGTGACGG‑TTTGGTGAGGGCTTGat                                                                                                                                  <  2:42213/242‑1 (MQ=255)                                                                                                   ctgATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATAtttat                                                                                                                            >  1:225186/1‑248 (MQ=255)                                                                                                               ccGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTTGTACCCGCAGATGTAGACGTTACTCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCGCAACAATCGCCGTTGCTTCAGTGGCTGGTGCATCAAAAGCTGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTa                                                                                                                 <  1:427984/250‑1 (MQ=255)                                                                                                                     ctgGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCCGAGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGCTGGCTTGGTATTTATTATCCCTGTTAAATtgtg                                                                                                          >  1:104799/1‑251 (MQ=255)                                                                                                                     ctgGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGTAGCGACGG‑GTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATtgtg                                                                                                          >  1:518878/1‑251 (MQ=255)                                                                                                                      gggCTAACAATGGTGACTGAC‑TGAGGAATATCACGCTGAG‑GTATCTCCATTTTGATACCCGAAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCAACTGGTGCTGTGACTTAACCCTCAACAATCACCGTTTCTACAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTTCTATGCAACCGGCAAGTAGTGACGG‑TGTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTgtgt                                                                                                         <  2:259954/250‑1 (MQ=255)                                                                                                                        gCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTg                                                                                                       <  1:565497/251‑1 (MQ=255)                                                                                                                           aaCAATAGTTACCGACGTG‑GGAATATCTCGGTTAG‑TAATCTGCATTTGGTTACCCGCATAGGTAGACGTTTCGCTGTAATTATATTCGCCATCATCTGGAGCTAGGACTTAACCCTCAACAAGCACCGTTTCTTCAGGGGCTGGTGCAGTAAAAGCGGCAGAAGGTAATAGTGCCAGGGTTATGCAACCGGCAAGTAGTGACGT‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTTTGTTGaaa                                                                                                    <  2:257967/251‑1 (MQ=255)                                                                                                                                 aGTGACCAAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAATAGGCAGAAGTAAAAAGTGCACGGGCTATGCAACCCGCGAGAAGGCGCGG‑AGTGGTGATAGCTTTAAATTGTTTATCCCTGTTAAACTGTGTTGAGAGCAttt                                                                                              >  2:619129/1‑251 (MQ=255)                                                                                                                                     aCCGAC‑TGAGGAATCTCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTACATTGTGTTGAAAGCATTTGaa                                                                                           >  1:516024/1‑250 (MQ=255)                                                                                                                                             gAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTg                                                                                   <  1:520682/251‑1 (MQ=255)                                                                                                                                                              g‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAg                                                                  <  2:60445/251‑1 (MQ=255)                                                                                                                                                                 cctctgcgttgtgttaacgccAGAGGTATACGTTACGCTGTTATCATGTGCGCCATCATCTGGAGCTTTGACTGAACCCTCAACAAGCACCTTTTCTTCAGTGGCTGGGGCAGCAAAAGCGTCAGAAGGTAATAGTTCCAGTGCTATTCAATCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCTCTGTTAAGTTGTTTTGAACGCATTTGAAATCCCTTTGTTTATGCTGTCTTCAgg                                                                 <  2:103495/230‑1 (MQ=255)                                                                                                                                                                    cTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTc                                                              >  1:163635/1‑250 (MQ=255)                                                                                                                                                                       cATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGCAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTTATTATCCCTGTTAAATTTTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGc                                                          >  2:252978/1‑251 (MQ=255)                                                                                                                                                                        aTTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGGAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGcc                                                         >  1:77602/1‑251 (MQ=255)                                                                                                                                                                          tttGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCg                                                        <  1:375753/250‑1 (MQ=255)                                                                                                                                                                             ggTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCggggg                                                    >  1:113887/1‑248 (MQ=255)                                                                                                                                                                             ggTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGCGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCggagg                                                    <  1:502470/251‑1 (MQ=255)                                                                                                                                                                                   cGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGGCgggtt                                                                                                                                                 >  2:119170/1‑150 (MQ=255)                                                                                                                                                                                   cGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑ttt                                                                                                                                                <  1:119170/153‑1 (MQ=255)                                                                                                                                                                                      agagGTAGACGTGACGCTGTAATCAGTTTCTCCATCATCTGGAGCTGTGACTGAACCCTCAACACGCTCCGTTTCTGTAGTGGCTGGTGCAGCAAAAGCGGCAGAAGTTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACTG‑TTTGGTGATGGCTTGATATTGATTAGCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGTAGGAGGGtgatg                                           <  2:590572/251‑1 (MQ=255)                                                                                                                                                                                       gagGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGtgatga                                          <  1:21149/251‑1 (MQ=255)                                                                                                                                                                                         ggTAGACTTTACGCGTTTATCATCTACGCCATCAGCTGGATCTGTGACTGAACCCTCGACAAGCACCGTTTCTTTAGTGGCTGTTGAAGCAAAAACGGCAGAAGTTAATAGTGCCAGGGCTATGCAACCGGCAATTAGTGACGT‑TGTGGTGATGGTTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGAATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCAGGCCTGAGGTGGGTGATGAga                                        <  2:561918/251‑2 (MQ=255)                                                                                                                                                                                             gACGTTACGCTGTAAGCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGAtt                                    <  1:341100/251‑1 (MQ=255)                                                                                                                                                                                                gTTACGCTGTAATCATTTTCTGCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAATTCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATtata                                 <  2:560389/251‑1 (MQ=255)                                                                                                                                                                                                   aCGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATAttt                              <  1:252978/251‑1 (MQ=255)                                                                                                                                                                                                        gTAATCATTTTCGCCATCATCTGGAGCTGTTACGGAACCCGCAACAATTATCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCATAAGGGAATAGGTCCTGTGTTATGCAACGGGCAAGTAGTGACGG‑GTTTGTGATGGCTTGATATTGATTATCCCTGTTAAATGGTGGAGAAATGATTTGAAATCTCTTGTTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGCGGGATGTGCGATTATATGTAgtgt                         <  2:644329/251‑1 (MQ=255)                                                                                                                                                                                                             cATTTTCGCCATCATCGGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGa                     <  2:77602/250‑1 (MQ=255)                                                                                                                                                                                                                     ccATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATtttg            <  1:195428/251‑1 (MQ=255)                                                                                                                                                                                                                         catcTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTgtc                                                                       >  1:501040/1‑188 (MQ=255)                                                                                                                                                                                                                         catcTGGAGCTGTGACTGAACCCTCAACAATCACCGGTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTgtc                                                                       <  2:501040/188‑1 (MQ=255)                                                                                                                                                                                                                             tGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGGGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTaa     >  1:564578/1‑250 (MQ=255)                                                                                                                                                                                                                              ggAGCTGTGACTTAACCCTCAACAATCACCGTTTCGTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGGCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAAt   <  1:18017/251‑1 (MQ=255)                                                                                                                                                                                                                              ggAGCTGTGACTGAACCCGCAACAATCACCGTTTCTTCAGTGGCGGGTGCAGGAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCGCATGCCGGAGGAGGGTGATGAGCGATTATATTTTGGGTGCGAATAATTTTGTGTGTAAAt   <  2:65073/251‑1 (MQ=255)                                                                                                                                                                                                                               gtgCTGTGACTGAACCCTCAACAATAACCGTTTCTTCAGTGTCCTGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCATTGCTATGCAACCGGCAAGTAGTTACGG‑TTTGGTGATTGCTTGATATTGATTAGCCCTGTTAAATTGTGTTGAAAGCATTTGAATTCTCTTGGTTTATTTTGTCGTCAGGCTAATGCCTGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATg  <  2:128580/249‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAC‑TGAGGAATATCACGTTGAG‑TCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGG‑TTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATG  >  NC_000913/1161175‑1161645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑