Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,709	A→G	V791V (GTA→GTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,709	0	A	G	100.0%	204.2 / NA	66	V791V (GTA→GTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/28);  total (38/28)

TAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACG  >  NC_000913/2463‑2952                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    taacgttgagggtattggtGCCTCTTTGATGGTGAATCTGTCCCAGCTCGATGACCTCTTGGCCCGGCGAGGGGCGAAGGCTCATTATGAAGGAAAAGTTTTTAGCTATGTTGGCAAGATTGCTGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAAGGATCCGCTGTTCAAAGTGAACAAGGGCGTAAACGCCCTGGCATTTTATAGATACTATTATGAGGCGATGCCGTTGGTGCTg                                                                                                                                                                                                                                                  <  2:89258/232‑1 (MQ=255)  aaCGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGtgctgc                                                                                                                                                                                                                                                 <  2:611759/250‑1 (MQ=255)   aCGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGCAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGCAAACGCCCTGGCCTTTTATACCCACTATTATCAGCCGCTGCCGTAGGTGCTgcgc                                                                                                                                                                                                                                               >  1:652199/1‑251 (MQ=255)       tgAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATATCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCggt                                                                                                                                                                                                                                            >  1:34952/2‑249 (MQ=255)           ggTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATAGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATgg                                                                                                                                                                                                                                       >  1:73643/1‑251 (MQ=255)             tGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTg                                                                                                                                                                                                                                     >  1:391545/1‑251 (MQ=255)              gttggtGCCTCTTTTATGTCGAAGCTGTCACAGCTAGACGATCTCTTGGCCGCGCGCGTGGCGAAGGCCCGTGATGAATGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTATAGAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGc                                                                                                                                                                                                                                    <  2:423890/246‑1 (MQ=255)               gttttGCCGCGTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCg                                                                                                                                                                                                                                   <  2:186987/248‑1 (MQ=255)                  ttGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGCAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGTTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGGGAACAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCCGCCACTGCCGTTGGTGCTGCGCGGATATGGTGCGGGc                                                                                                                                                                                                                                >  1:451180/1‑251 (MQ=255)                      cGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATg                                                                                                                                                                                                                            <  1:385953/251‑1 (MQ=255)                               ggCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGCTGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGCTCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTCTAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTAc                                                                                                                                                                                                                     >  2:225365/1‑249 (MQ=255)                                gCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGc                                                                                                                                                                                                                  >  1:309328/1‑251 (MQ=255)                                 cGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCt                                                                                                                                                                                                                 >  1:502664/1‑251 (MQ=255)                                            cagcTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAACAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCtt                                                                                                                                                                                                      >  1:205255/4‑251 (MQ=255)                                               cTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCCCTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTTCCGGGGGCTTTGc                                                                                                                                                                                                   >  2:601582/1‑251 (MQ=255)                                                tCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCAGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCCGGCAATGACGTTACAGCTGCCGGTGTCTTTGcc                                                                                                                                                                                                  >  1:381481/1‑250 (MQ=255)                                                 cgacgaTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGccg                                                                                                                                                                                                 >  1:631281/1‑249 (MQ=255)                                                  gacgaTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGccga                                                                                                                                                                                                <  1:293131/251‑4 (MQ=255)                                                      aTCTCTTTGCCGCTGGCTTGGATAAGGCCCGTGTAGAAGGAAAATTTTTGCGCTATGTTGGCAATATTGATTAAGATGGTGCCTGCCGCGTGAAGATTGCCGAATTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCTCTATTAGCAGCCGCTTCCGTGGGTGCTGCGCGGGTATGGTGCGGGCAATGACGTTACAGCTTCCGGTGGCTTTGCCGATc                                                                                                                                                                                              <  2:652199/249‑1 (MQ=255)                                                          ctTTGCCGCGCGCGTGGCGAATGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCt                                                                                                                                                                                             <  1:24331/246‑1 (MQ=255)                                                          ctTTGCCGAGCGCGGGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGAGCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCt                                                                                                                                                                                             <  2:8201/246‑1 (MQ=255)                                                            ttgacgcgcgcgTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGAGTGAAGAATGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTa                                                                                                                                                                                         <  2:278477/244‑1 (MQ=255)                                                                  cgggcgTGGCGAAGGCCCGTGAGGAAGGAAAAGTTTGGCGCTATGTGGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGATGTGGATGGTAATGAGCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCct                                                                                                                                                                                 <  2:291882/247‑1 (MQ=255)                                                                            gAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGt                                                                                                                                                                      >  1:251103/1‑251 (MQ=255)                                                                                   ccgctcttcctatctgttgtGCGTTATGTTTGCAATATTGGTGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGt                                                                                                                                                               <  2:133250/232‑1 (MQ=255)                                                                                       atgaAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTg                                                                                                                                                            >  1:601277/1‑250 (MQ=255)                                                                                          aaGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACAt                                                                                                                                                        <  1:100415/251‑1 (MQ=255)                                                                                            ggAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGATGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTGGGAGTCTGACATgg                                                                                                                                                      >  2:180342/1‑251 (MQ=255)                                                                                              aaaaGGTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCGCTCATGGAAGTTAGGAGTCTGACATGGtt                                                                                                                                                    <  1:225365/251‑1 (MQ=255)                                                                                                  gTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGt                                                                                                                                                               >  1:133250/1‑236 (MQ=255)                                                                                                    tttGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGtt                                                                                                                                              >  2:305415/1‑251 (MQ=255)                                                                                                                 ggCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCtt                                                                                                                                 >  1:190824/1‑251 (MQ=255)                                                                                                                      tatTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGt                                                                                                                            >  1:460251/1‑251 (MQ=255)                                                                                                                       atTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGt                                                                                                                            <  1:305415/250‑1 (MQ=255)                                                                                                                             gaAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAat                                                                                                                      >  1:575287/1‑250 (MQ=255)                                                                                                                                      gccTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCg                                                                                                            >  1:379228/3‑251 (MQ=255)                                                                                                                                      gccTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCg                                                                                                            >  2:207748/3‑251 (MQ=255)                                                                                                                                        cTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGACGTTAGGAGTATGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGAGTCggg                                                                                                          >  1:27894/1‑251 (MQ=255)                                                                                                                                                    gATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTACGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTAACCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTc                                                                                              >  1:94729/1‑251 (MQ=255)                                                                                                                                                     aTTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCg                                                                                             >  1:617875/1‑251 (MQ=255)                                                                                                                                                        gacGAAGTGGAGGTTAATGATCCGCTGCTCAAAGTGTAAAATGGCGAAATCGCCCGGGTCGGTTATAGCAACTATTATCAGCCGCTGCCGTTGGGGCGGCGCGGATAGGGTGCGGGCAATGACGTTACAGTTGCCGGGGTCTTTGCAGATATGGTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCTCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGAGGTGCTTgggg                                                                                          <  2:451180/249‑1 (MQ=255)                                                                                                                                                            aaGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGggcggc                                                                                      >  1:455118/1‑251 (MQ=255)                                                                                                                                                               tGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCAGCTGCCGCTGGAGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGGGTCTTTGGCGATCTGCTACGACCCCGATCATGGGAGTTAGGAGTCTGACATGGTTATAGTTTATGCCCCGGCTTCCAGTGCCATTATGAGCGTCGGGTTTGCTGTGCTCGGGGcggcggg                                                                                   >  2:176281/1‑250 (MQ=255)                                                                                                                                                                ggATGGGAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTg                                                                                  <  2:601277/251‑1 (MQ=255)                                                                                                                                                                    ggTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGaca                                                                               <  1:151121/250‑1 (MQ=255)                                                                                                                                                                            tCCGCTGTTCAAAGTGCAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCAGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCCCATGGAAGTTAGGAGTCTGACATGGTTACAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGAGTCGGGTTTGATGTGCACGGGGCGGCAGTGaacacggttgat                                                                      >  2:301041/1‑240 (MQ=255)                                                                                                                                                                            tCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGc                                                                                                                <  1:581244/209‑1 (MQ=255)                                                                                                                                                                            tCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGc                                                                                                                >  2:581244/1‑209 (MQ=255)                                                                                                                                                                                 tGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGa                                                                                                                                                           <  2:392542/161‑1 (MQ=255)                                                                                                                                                                                 tGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGa                                                                                                                                                           >  1:392542/1‑161 (MQ=255)                                                                                                                                                                                     cAAAGTGAAAAATGGCGAAAACTCCCTGGCCTTTTATAGCCACTATGATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTg                                                             <  1:364040/251‑1 (MQ=255)                                                                                                                                                                                              aaaTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGGGAt                                                    >  1:500776/1‑251 (MQ=255)                                                                                                                                                                                                aTGGCGAAAACGCCATGGCCTTTTATAGACACTATTATCAGCCGCTGCCAGTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTCCCGGGGTATTTGCCGATCTGCTACGAAACCTCTCTTGGAAGTTAGGAGTCTGACATGGATAAAGTTAATGCCCCGGCTTCCAGAGCAAATATGAGCGTAGGGTTTAATGTGCGCGGGTCGGCGAGGGCACCCGTTGATGGTGcgatgtctggggcggt                                                  >  2:406982/1‑236 (MQ=255)                                                                                                                                                                                                  ggCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGt                                                  >  1:428810/1‑249 (MQ=255)                                                                                                                                                                                                        aacgccctggctgttattaGCCACTATTATCATACGCGGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAGTGACGTGACAGCTGCCGGTGTCTGTGCCGATTGGCTACGTACCCTCTCATGGAAGGTAGGAGTCTGACATGGTTAAAGTGTATGCCCCGGCTTCCAGTGCCAATATGAGCGTTGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACgg                                          <  2:379228/234‑1 (MQ=255)                                                                                                                                                                                                           gCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTg                                       <  1:296470/251‑1 (MQ=255)                                                                                                                                                                                                              cTGGCCTTTTATAGCCACTATTATCAGACGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACACTCTCATGGCAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTAGGGGCGGCGGGGACACCCGTTGATGGTGCATTGCTTGGAGATGTAGTAACGGTTgggg                                    >  2:398468/1‑248 (MQ=255)                                                                                                                                                                                                                ggCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAggc                                   <  1:80135/250‑1 (MQ=255)                                                                                                                                                                                                                 gCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGcggcgg                                 >  1:370757/1‑251 (MQ=255)                                                                                                                                                                                                                   cttttataGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGACGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGATTCCAGTGCCAATATGAGCGTCGGGTTTGATGGGCTCGGGGCGGCGGGGACACCTGTTGATGGGGCATTGCTCGGAGATGTAGACACGGTTGGggcggc                                >  2:416797/5‑250 (MQ=255)                                                                                                                                                                                                                             cACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCa                     <  1:353139/251‑1 (MQ=255)                                                                                                                                                                                                                                 attatCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGt                   <  2:251103/249‑1 (MQ=255)                                                                                                                                                                                                                                 attagCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGt                                               <  1:428272/221‑1 (MQ=255)                                                                                                                                                                                                                                      cAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTcaac             >  1:649352/1‑250 (MQ=255)                                                                                                                                                                                                                                         ccGCTGCCGTTGGTGCTGCGCGTATATGGTGCGGGCAAAGTCTTTACAGCTGCCGGTTTATTTGCCGATCTGCTACGACCCCTCCCATTGAAGTTAGGAGTCTGACATGGTTAAAGGTTATGCCCCGGCTTCGAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTTCATGGCTCGGAGATCTAGGCACGGTTGAGTCTGCAGCGACATGCAGTCCCAACAAcc         <  2:33220/251‑1 (MQ=255)                                                                                                                                                                                                                                            cTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGGTGGTGCATTGATCGGAGCTGTAGTCACGGTTGAGGCGGCCGAGACATTCAGTCTCAACAAccccg      >  2:137465/1‑248 (MQ=255)                                                                                                                                                                                                                                                cGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATTGAAGTTAGGAGTCTGACATGGTTAAAGGGTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGAct  <  1:398468/251‑2 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACG  >  NC_000913/2463‑2952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑