Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,974	C→T	A12V (GCT→GTT)	rluC →	23S rRNA pseudouridine(955/2504/2580) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,974	0	C	T	100.0%	111.6 / NA	35	A12V (GCT→GTT)	rluC	23S rRNA pseudouridine(955/2504/2580) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/21);  total (14/21)

CCACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCG  >  NC_000913/1144741‑1145215                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  ccACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAg                                                                                                                                                                                                                                  <  1:676425/251‑1 (MQ=255)          cccTTGTTATAGCAAGATGTCTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACGCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAAc                                                                                                                                                                                                                         <  2:307184/250‑1 (MQ=255)                ttATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTGCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATc                                                                                                                                                                                                                    <  2:307660/250‑1 (MQ=255)                        aGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGACTCCTGTTGGCTAATTAGAATCGCCAACCATGACAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGCCAACGTATCGATAACttt                                                                                                                                                                                                           >  2:335263/1‑251 (MQ=255)                            gACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTg                                                                                                                                                                                                        <  1:208314/250‑1 (MQ=255)                            gACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGc                                                                                                                                                                                                       <  1:459413/251‑1 (MQ=255)                                gttACCATTTATAACCCTCTTACTCACAGGTTTTTCACTTCTGTCGTGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATATAAAAATGAGTGGCGCTAATCCTGTTGTCGAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGTAGCGGGTCAACGTATCGATATCTTTTTTCGTa                                                                                                                                                                                                    <  2:224692/249‑1 (MQ=255)                                              cccacTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAACGTGATCACGCTGTGAAGAAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAACAAGAGACTCCATACGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAAATTTTTGCGTAACCAATTGACAGGCg                                                                                                                                                                                     >  2:398930/5‑251 (MQ=255)                                                               ttttCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGAtt                                                                                                                                                                    >  1:615078/1‑251 (MQ=255)                                                                tttCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGAtt                                                                                                                                                                    <  1:420800/250‑1 (MQ=255)                                                                        cttGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGACGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGGACCAAAAAGTATGATTTACCGTAtt                                                                                                                                                           >  2:7034/1‑251 (MQ=255)                                                                                   ttGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAgg                                                                                                                                                >  1:525721/1‑251 (MQ=255)                                                                                     ggTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCg                                                                                                                                              <  1:565232/251‑1 (MQ=255)                                                                                               ccATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGt                                                                                                                                    <  2:615078/251‑1 (MQ=255)                                                                                                cATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTg                                                                                                                                   <  1:287396/251‑1 (MQ=255)                                                                                                 aTCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTa                                                                                                                                                             >  1:473981/1‑224 (MQ=255)                                                                                                       aaaGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCAGCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTGCCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACaaaa                                                                                                                            <  1:393626/251‑1 (MQ=255)                                                                                                               cACGCAGTGATGTCATAAGGTGCAACAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCCATTGAATGGCGTACCAAACAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGTGTGAACAAAAAACGTAtt                                                                                                                    >  2:439513/1‑251 (MQ=255)                                                                                                                           taataaGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCCATTGAAAGGCGTACCCAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAatat                                                                                                        >  1:438284/1‑251 (MQ=255)                                                                                                                                          gTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCgggg                                                                                         >  1:313017/1‑250 (MQ=255)                                                                                                                                          gTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGt                                                                                         <  1:200024/251‑1 (MQ=255)                                                                                                                                                  aaGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGt                                                                                 <  1:439513/251‑1 (MQ=255)                                                                                                                                                                gAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCg                                                                                                                                                               <  1:464999/159‑1 (MQ=255)                                                                                                                                                                gAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCg                                                                                                                                                               >  2:464999/1‑159 (MQ=255)                                                                                                                                                                gAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATACAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCg                                                                    <  1:139482/250‑1 (MQ=255)                                                                                                                                                                  taggcacgAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGATGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATACCTTTTTGCGTACCCAATTGAAAGTCGTACCAAAAAGTATGATTTACCGTCTTTTGCGTAAGAGCGAAGAGCGGGGGAACAAAAAACGTATTAAGCATGAATATAAACTCGGATCGGGGGATGAGGTGCGGATTCCACCGGtt                                                                 >  2:244440/7‑251 (MQ=255)                                                                                                                                                                                gTTGGCTAAGTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCg                                                   <  2:314111/251‑1 (MQ=255)                                                                                                                                                                                           aGAATCGCCAACCATGAAAACAGAAACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGAGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGAATGATTTACCGTATTTTGCGGAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGACTATAAACTAGAAGCGGAGGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGCAGAAGAgg                                        >  2:21837/1‑251 (MQ=255)                                                                                                                                                                                             aagcGCCAACCATGAAAACAGAGACTCGATCCGTAAAAATTTGTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCg                                      <  2:467486/248‑1 (MQ=255)                                                                                                                                                                                                 gCCAACCATGAAAACAGAGACGCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTGTTGCGTTCCCGATTGAAAGGCGTACCAAAAAGGATGATTTACCGTa                                                                                                                                                             <  1:185407/128‑1 (MQ=255)                                                                                                                                                                                                  ccacacATGAAAACAGAGACTCCATCCGTAAAAAGTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCGCCGGTGCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTc                                 <  1:4639/246‑1 (MQ=255)                                                                                                                                                                                                     aCCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGcc                              <  1:233215/251‑1 (MQ=255)                                                                                                                                                                                                                 gagaCTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACCTCTGCaaa                   >  1:230934/1‑250 (MQ=255)                                                                                                                                                                                                                       ccATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGGCCCATCTGCaaaaaggggg            >  1:290289/1‑245 (MQ=255)                                                                                                                                                                                                                                 aaaTTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCg  <  1:87688/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  CCACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCG  >  NC_000913/1144741‑1145215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑