Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,141,865	G→A	P835S (CCG→TCG)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,141,865	0	G	A	95.5%	248.6 / ‑2.5	91	P835S (CCG→TCG)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (50/34);  total (55/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTG  >  NC_000913/1141624‑1142102                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ggATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACgcgc                                                                                                                                                                                                                                       >  1:660177/1‑250 (MQ=255)       gtcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGCCAAGGACCTCCGTCCACC‑TCGGCTGCCCATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTTTAACTCTTGCGGACGTACAATTGGTAAGCGGATCCCGACTTTGCCAGAGGCCCGGTCCGAAGACgcgccccctcc                                                                                                                                                                                                                                >  2:290407/1‑244 (MQ=255)       gtcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTAc                                                                                                                                                                                                                                <  1:445309/251‑1 (MQ=255)              cAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAAc                                                                                                                                                                                                                         >  1:222548/1‑251 (MQ=255)                  cACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGc                                                                                                                                                                                                                      >  1:491277/1‑250 (MQ=255)                       ccGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGTACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTgg                                                                                                                                                                                                                 <  1:650310/250‑1 (MQ=255)                       ccGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCAAGCTACGGTCAACGGCATTggg                                                                                                                                                                                                                >  1:651924/1‑250 (MQ=255)                        cgggtGCGGTTCGGCAACCTGACCGGGGGCTTTTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGATGCCAGTTCCGAAGACGCGCCCGCTACGGTCAACGGCATTGGCg                                                                                                                                                                                                               <  2:483568/247‑1 (MQ=255)                                gTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGtt                                                                                                                                                                                                       <  1:290407/251‑1 (MQ=255)                                    ggCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTTCACATTTACTTCTTCCTGTTCGCGCTGCTCTTCCACCTGTACATCTTTCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCCAGGACGCGCACGCTACGGTCAACGGCATTGGGGACTGGGTTGGAt                                                                                                                                                                                                   >  1:130322/1‑251 (MQ=255)                                        aCCTGAACGGGGGTTTCTACGACAACGGCACCTTCTTCAACAACTGGCGCGCTAACATCCGGTGCGATAGCGGCGGCGACAGGGACCTCAGTC‑GACATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGGACAATTGGATAGCGGATCCAGACTTTTCCAGAGGCCAGTTCCGAATACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACg                                                                                                                                                                                               <  2:539493/251‑1 (MQ=255)                                              aCGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCCACCTGTACATCTTGCGGGCGTACAATTGGATAGCGGGTCCAGACTTTGCCCGAGGCCAGGTCCGAAGACGCGCCAGCTACGGGCAACGGCATTTGCGACGGGGGTGGGTAACGCCCGTc                                                                                                                                                                                         >  2:436338/1‑251 (MQ=255)                                                  gggCTACTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGTGTCCAGACTTTTCCAGAGGCCAGTTCCGAAGACGCGCACGCTCCGGTCAACGGCATTGACGACTgtgatggcagatcggaatggcac                                                                                                                                                                                     >  1:459912/1‑229 (MQ=255)                                                      ttCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGc                                                                                                                                                                                 >  1:61380/1‑251 (MQ=255)                                                          aCGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCAGTGGCGACTGGGTTGGATAACGCTCGTCACGATAGcgac                                                                                                                                                                              <  1:651425/250‑1 (MQ=255)                                                                ccGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGacgacgacg                                                                                                                                                                       <  1:15293/251‑1 (MQ=255)                                                                   gCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCCGTC‑ACCATCGGCTTCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAAATGGCTAACGGATCCAGACTTTGCCCGAGGCCAGTTCCGAAGACGCGCCCACTACGGGCAACGGCCTTGGGCAATGGGGTGGATCACGCACGTCActgacgccacggcgaaccc                                                                                                                                                                     >  2:307892/1‑232 (MQ=255)                                                                      aCTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGAcc                                                                                                                                                                 <  1:642496/251‑1 (MQ=255)                                                                        ttcttcAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGGTCCAGACTTTGCCAGAAGCCAGTTCCGAAGACGCGCACGCGACGGTCAACGGCATTGGCGGATGGGTTGGGTAACGCCCGTCACGATAGGGACGACGGCGCTggcccc                                                                                                                                                               >  2:234579/1‑246 (MQ=255)                                                                             caacaaCTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACAAGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGGACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCCGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGAGACTGGGTTGGATAACGCTCGTCACGATAGCGACGAAGAAGATGACCCCTTAcg                                                                                                                                                          >  1:656244/1‑251 (MQ=255)                                                                                    tGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATATTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCAATTACGCGCAggg                                                                                                                                                   >  1:655758/1‑250 (MQ=255)                                                                                     ggCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTGAGTC‑ACCATCGGCTGCAAATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGGGGCCAGTTACGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGGCACGATAGCGACGACGACGCTGGCCACTTACGCGCAGGTg                                                                                                                                                  >  1:177387/1‑251 (MQ=255)                                                                                      gcgcgctatccacctgtcctaTAGCGGCGGCGACAGGGACCTCAGTC‑TCCTTCGGCTGCACATGTTCTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTCCATCTTGCGGACGTACAATTGGATAGCGGACCCAGACTGTGCCAGAGGCCAGGTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTCCGCGCAGGTGa                                                                                                                                                 <  2:578465/231‑1 (MQ=255)                                                                                       cgcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGAc                                                                                                                                                <  1:243482/251‑1 (MQ=255)                                                                                        gcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACg                                                                                                                                               <  1:436338/251‑1 (MQ=255)                                                                                          gcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAg                                                                                                                                             <  1:157053/251‑1 (MQ=255)                                                                                          gcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACATCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGGTCCAGACTTTGCCAGAGGCCAGTTCCGACGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGCTAGCGACGCCGGCGCTGACCACTTCCGCGCAGTTGagggg                                                                                                                                             >  1:168010/1‑247 (MQ=255)                                                                                            tAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACggggg                                                                                                                                           >  1:148806/1‑247 (MQ=255)                                                                                                aacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgc                                                                                                                                       >  1:615374/1‑251 (MQ=255)                                                                                                          aTAGTGGCGGCGACAGAGACCTCAGTC‑ACCATCAGCTGCACATGTACTTCTTCCCGTTCGCCCTGCTCTTCAACCTGGACCTCTTGCGGACGTACCATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGGTCCGGAGGCGCGCACGCTACGGTCCACAGGATTGTCGCCTGGGTTTGATAACGCTCGTCCCGCTAGCGACGGCGACGCTGACCACTTTTGCGCAGGTGACGACGCCAGCGGCgagagcg                                                                                                                             >  2:229728/1‑248 (MQ=255)                                                                                                             gcggcggcgACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAacgacg                                                                                                                          <  1:664294/251‑1 (MQ=255)                                                                                                                  ggcgACAGGGACATCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGATCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGACAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTAAACGGCATTGGCGACTGGGTTGGATCACGCTCGTCACGATAGCGACGACGACGCTGACAAATAACGCGCAGGAGACGAGGCGCGCGGCgagacccacgtggc                                                                                                                      >  2:678581/1‑240 (MQ=255)                                                                                                                   ggggaccgggtcctcatgccccATCGGCTGAACATGTACTGCTTCCTGGTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTTCAATTGGATATCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACTGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGTGGCa                                                                                                                     <  2:247647/231‑1 (MQ=255)                                                                                                                      aCAGGGCCCACAGTC‑CCCATCTGCTGCACTTTTACTTCGTCCTGTTCGCGCTGTGCCTCAACCCGTACATCTTGCGGACGTACAATTGGATATCGCCCCCCGACTTTGCCAGACGCCAGTTCCGCAGACGCGCTCGCTACGGTCTACGGCATCGGCGACTGGGTTGGATAACGCTCTTCACTATAGCGACGTCGACGCTGACCACTTACGCGCCGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGcc                                                                                                                 <  2:177387/251‑1 (MQ=255)                                                                                                                       cAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCAAGATAGCGACGACGACGCTGACCACTTACGCGAAGGTGACGAGGCGAGCGGCGAGCACGACGCGGCATGccc                                                                                                                >  2:589161/1‑250 (MQ=255)                                                                                                                        aGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAc                                                                                                               >  1:433445/1‑251 (MQ=255)                                                                                                                         gggACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGc                                                                                                                                                                                                                >  2:167097/1‑153 (MQ=255)                                                                                                                         gggACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGc                                                                                                                                                                                                                <  1:167097/153‑1 (MQ=255)                                                                                                                         gggACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCGTCAACCTGTACATCTTGCGGACGTTCAATTGGAGAGCGGATCCAGACGTTGCCAGAGGCCAGTTCCGAAGACGCGCTCGCTGCGGTCAACGGCATTGGGGACTGGTTTGGATAACGCGCGTCACGATAGCGACGACGGCGGTGAGCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAcc                                                                                                              <  1:395427/251‑1 (MQ=255)                                                                                                                         gggACCGCAGTC‑CCCATCGGCTGCCCATGTACTGCTTCCTGTTCGCGCTGCTATTCAACCTGTACATCTGGCGGACGGTCAATTGGATAGCGGATCCAGACGTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGGCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAcc                                                                                                              <  2:222548/251‑1 (MQ=255)                                                                                                                            aCCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGtt                                                                                                           >  1:569547/1‑251 (MQ=255)                                                                                                                            aCCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCGGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGtt                                                                                                           <  1:115359/251‑1 (MQ=255)                                                                                                                                 aGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCTCGCTGCTCTTCAACCTGTACATCTTGCGGACTTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGGCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAcc                                                                                                              <  2:200574/243‑1 (MQ=255)                                                                                                                                 aGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAcc                                                                                                              >  1:200574/1‑243 (MQ=255)                                                                                                                                   tC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTAAGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACgg                                                                                                    >  1:467447/1‑251 (MQ=255)                                                                                                                                    c‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGCGAACGACGCGGCATGCCACCGTTGTCACGGt                                                                                                   >  1:101243/1‑251 (MQ=255)                                                                                                                                       ccATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTa                                                                                                 <  1:589161/251‑1 (MQ=255)                                                                                                                                        cATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAAGTGGATAGCGGATCCAGACGTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCGCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGGGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCTCCGTTGTCACGGGTAt                                                                                                <  1:578425/251‑1 (MQ=255)                                                                                                                                              cTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCAt                                                                                          >  1:330369/1‑251 (MQ=255)                                                                                                                                                 cacaTGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGt                                                                                       >  1:576568/1‑251 (MQ=255)                                                                                                                                                   caTGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTtc                                                                                     >  1:543246/1‑251 (MQ=255)                                                                                                                                                     ggttagtcttcCTGTGCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCCCGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCAGTGTtctc                                                                                   <  2:104215/245‑1 (MQ=255)                                                                                                                                                        aCTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCtcttc                                                                                <  1:20222/251‑1 (MQ=255)                                                                                                                                                          ttcttcCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCCCTTACGCGCAGGTGACGCGGCGGGAGGCGAGCACGACGCGGCATGCCACCGTTGGCACGGTTATCATCCTTGTTCtcttcat                                                                              >  2:498859/1‑249 (MQ=255)                                                                                                                                                                ccgttcgcctttctcctCAACCTGTTCGTCGTGCGGCCGTACAATTGGATTGCGGCTCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACTCTACGGTCAACGGCATTGGCGACTGTGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGTCCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACTACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTTCTGtt                                                                        <  2:460511/235‑1 (MQ=255)                                                                                                                                                                 tGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGATGGATAACGCTCGTCACGATAGCAACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGTCATGCCACCGTTGTCACGGTTATCAGCATTGTTATCTTCTTGCTGTTc                                                                       >  1:652964/1‑251 (MQ=255)                                                                                                                                                                   ttCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGAGCACGCTACGGACAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTCCGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCAAGCCACCGTTGGCACGGGTATCAGCATTATTCTCTTCTTGCTGTTCTg                                                                     >  2:90108/1‑251 (MQ=255)                                                                                                                                                                     cgcgCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAAAGCTCGTCACGATAGCGACGCCGACGCTGACCACTTCCGCGCAGGTGACGAGGCGAGCGGCGAGCACGACGCGGCACGCCACCGTTTGCACGTTTATCAGCTTGGTTCTCTTCTTGCTGTTCTgtt                                                                   >  2:504158/1‑249 (MQ=255)                                                                                                                                                                        gctgctCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGCAGACGCGCACGCTACGGTCAACGGCATTGGCGTCTGGGTTGGATAACGCTCGTCACGATAGCGCCGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGAGGCGAGAACGACGCGGAATGCCACCGTAGTCACGGATATCAGCATTGTTCTCTTCTTGATGTTCTggagcc                                                                >  2:513407/1‑247 (MQ=255)                                                                                                                                                                           gctCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGtt                                                             <  1:437540/251‑1 (MQ=255)                                                                                                                                                                              ctTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTgc                                                          >  1:56302/1‑251 (MQ=255)                                                                                                                                                                               ttCAACCTTTACATCTTGCGGACGTACAATTGGATAGCGGATCCCGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGATACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCCCGATAACGACGACAACGCTGACCACTTAAGCGCAGGGGACGGGGCGAGCGGCGAGAACGACGAGGCATGCCACCGTTGGACCGGTTCTCAGCATTGTTATCTTCTTGCTGTTCGGGTGCCGTTTg                                                           >  2:585950/1‑249 (MQ=255)                                                                                                                                                                                    ccTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACg                                                    <  1:307892/251‑1 (MQ=255)                                                                                                                                                                                    ccTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGGTCCGAAGACCGGCACGCTACGGTCAACGGCATTGGCGACAGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGTGAGCGGCGAGAACGACGCGTCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTGCTTGCTGTTCTGGGGCAGTTTGCGCTACg                                                    <  2:535695/251‑1 (MQ=255)                                                                                                                                                                                       gTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGAc                                                  <  1:390927/250‑1 (MQ=255)                                                                                                                                                                                       gTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACt                                                 >  1:558048/1‑251 (MQ=255)                                                                                                                                                                                            tCTTGCGGACGTACAATTGGATGGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGCAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCGCTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTTCAGTTTGCGCTACGTCTGGc                                              >  1:421471/1‑249 (MQ=255)                                                                                                                                                                                            tCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGACGACGCGCACGCTACGGTCAACGGCATTTGCGACTGGGTTGGATAACGCTCGTCCCGATAACGACGCCGACGCTGACCACTTACGCGCAGGGTACGGGGCGAGCGGCGAGAACGACGCGGCATGCCACCGGTTTCACGGTTATCAGCATTGTTATCTTCATGCTGTTCTGGTGCAGTTTGCGCTAAGAATGGc                                              >  2:499725/1‑249 (MQ=255)                                                                                                                                                                                             cTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGACAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGCGAACGACGCGGCATGCCCCCGTTGTCACGGTTATCAGCCTTGTTCTCTTCTTGCTGTTCTGGTGCCGGTTGCGCTACGACTGGCAGc                                           >  2:529884/1‑251 (MQ=255)                                                                                                                                                                                                  ggACGTACAATTGGATATCGGATCCAGACTGTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCCCCGGTGTCACGGTTATCAGCATTGTTCGCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCggggc                                      <  2:433445/251‑3 (MQ=255)                                                                                                                                                                                                   gACGTACAATTGAATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATCGGCGACTGGGTTGGATAACGCTCGTCCCGATAGCGACGACGACGCTGACCACTTACGCGCAGGAGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCCCCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCTGTTTTCGCTACGCCTGGCCGCgggcat                                     >  2:393506/1‑248 (MQ=255)                                                                                                                                                                                                    aCGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCCGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGCACGACGCTGCATGCCAACGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGCATGGCAGCGGGCCtt                                    >  2:152985/1‑251 (MQ=255)                                                                                                                                                                                                    aCGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCAt                                                                                          >  1:623078/1‑197 (MQ=255)                                                                                                                                                                                                    aCGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCAt                                                                                          <  2:623078/197‑1 (MQ=255)                                                                                                                                                                                                    aCGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACtt                                    <  1:149811/251‑1 (MQ=255)                                                                                                                                                                                                      gTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGAAGACGCTGACCACTTACGCGCAGGGGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGATGTCACGGTTATCAGCATTGATATCTTCTTGCTGTTCTGGTGCAGTTTGCGCTAAGGCTGGCAGCGGGACTTTc                                  >  1:671758/1‑251 (MQ=255)                                                                                                                                                                                                        aCAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCAc                                <  1:180871/251‑1 (MQ=255)                                                                                                                                                                                                         cAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGCCGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTcccc                               >  2:530858/1‑248 (MQ=255)                                                                                                                                                                                                              ggATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCAACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAAAACGACGCGGCCTGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGttt                          >  2:269605/1‑250 (MQ=255)                                                                                                                                                                                                                     ggATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCgg                   >  1:453199/1‑250 (MQ=255)                                                                                                                                                                                                                     ggATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGGGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGGGCgg                   >  2:90257/1‑250 (MQ=255)                                                                                                                                                                                                                     ggATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGATATCAGCATTGATCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGTCTTTCACCAGTTcgttgcgt                   >  2:3162/1‑244 (MQ=255)                                                                                                                                                                                                                       aTCCAGACTTTTCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGTTCGTCACGATAGCGACGACGACGCTGACCACTTTCGCGCAGGTGACGTGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTGGCGCTACGACTGGCAGCGGGATTTTCACCAGTTCTGTGCGTgg                 <  2:655758/251‑1 (MQ=255)                                                                                                                                                                                                                           aGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGCTCAGTTTGGGCTACGACTGGCCGCGGGACTTTCACCAGTTTCGTGCGTGGTGCt             >  2:352555/1‑251 (MQ=255)                                                                                                                                                                                                                            gACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGCGAACGACGCGGCATGCCACCGTTGTCACGGTTATCCGCATTGTTCTCTTCTTGCTGTTCTGGTGCTGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCTAGGAGCTg            >  2:227459/1‑251 (MQ=255)                                                                                                                                                                                                                                ttGCCAGAGGACAGTGCCGAAGACGCGCACGCTACGGTCAACGGCATGGGCGACTGGGGTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGgcg         <  2:113014/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCtt      <  1:344983/251‑1 (MQ=255)                                                                                                                                                                                                                                    cAGAGGCCCGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGCAGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTTTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCCTTTTGCACTACGCCTGGCAACGGATCTTTCACCAGTTCtgtgtggggggcgggcacttcc    >  2:328087/1‑233 (MQ=255)                                                                                                                                                                                                                                    cAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTcc    >  1:111906/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAGAAGCCAGTTCCGAAGACGCGCAAGCTACGGTCAACGGCCTTGGCGACTGGGGTGGATACCGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGGCGAGGAGAGCGGCGAGAACTACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTACTGGTGCTGTTTGAGTGAGGACTGGCGGGGGGTCTTTCACCAGGTCTGTGCGTGGTGCTATCGCTTcc    >  2:293354/1‑251 (MQ=255)                                                                                                                                                                                                                                      gagGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTg  >  1:80988/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              GGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTC‑ACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTG  >  NC_000913/1141624‑1142102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑