Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,796	G→A	S12F (TCC→TTC)	flgN ←	flagellar biosynthesis protein FlgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,796	0	G	A	100.0%	172.9 / NA	57	S12F (TCC→TTC)	flgN	flagellar biosynthesis protein FlgN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/26);  total (31/26)

GTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGT  >  NC_000913/1129557‑1130029                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          gTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGACGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCAAAGCGAACATCTTGTCg                                                                                                                                                                                                                                >  2:175983/1‑251 (MQ=255)  ttGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCgg                                                                                                                                                                                                                               >  1:159629/1‑250 (MQ=255)       tCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGAt                                                                                                                                                                                                                           <  1:564333/250‑1 (MQ=255)        cATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGAtct                                                                                                                                                                                                                         >  1:23787/1‑251 (MQ=255)         aTTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGAtctc                                                                                                                                                                                                                        <  1:410291/251‑1 (MQ=255)             ggCGTAGTTGCTGCGTTTTCACAGGAATTTCCTGCCAGAGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTAGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGc                                                                                                                                                                                                                     >  2:419865/1‑250 (MQ=255)               cGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGAATTGGGTTCTTTCCTGCGTCACTGTTCGAGGCAATCCAGCGTCGCCAGCAGTGCGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTAGAGATCGCTAAGCACAGCGAACATCTGGTCGGGGATCTCTGCaa                                                                                                                                                                                                                   >  1:469539/1‑250 (MQ=255)                     tgctgcGTTTTCACAGTAATGTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACgtg                                                                                                                                                                                                             <  2:27386/250‑1 (MQ=255)                      gcttcGTTTTCACAGTAATTTCCTGCCAGCGGTGACTAATATCAACGCTATTTGCTGGATTGGGTTCTTTCCTGCGTAACTGCTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACgtgt                                                                                                                                                                                                            <  2:151109/246‑1 (MQ=255)                          cGTTTCCACAGTACTTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGAGCTGCCCCATAGAGATATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATAc                                                                                                                                                                                                       <  2:322010/251‑1 (MQ=255)                                cacaGTAATTTCCTGCCAGCGTTGACTAATCTCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATc                                                                                                                                                                                                   >  2:671959/1‑249 (MQ=255)                                 acaGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACgtgt                                                                                                                                                                                                            <  1:532274/239‑1 (MQ=255)                                 acaGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACgtgt                                                                                                                                                                                                            >  2:532274/1‑239 (MQ=255)                                      aatttcatgaaagagTTGACTAATATCAACGATATTTGCTGCATTGTGTTCTTTCCTGCGGAACTGTTCGAGGTAATCCAGCGTCGCCAGACGTGAGATTTTTTGTTCTGTCATACATTGCCACTGGCTGCCGTTGATCTGCCCCATAGAGAGCTGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTGATCCCAGCGAACATCTGGTCGCGGATCTCTGAAAGACATGTCAAACGCTCAGTTActc                                                                                                                                                                                           >  2:32711/15‑251 (MQ=255)                                       aTTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTActct                                                                                                                                                                                          <  1:214692/251‑1 (MQ=255)                                         ttCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGc                                                                                                                                                                                        >  1:290493/1‑251 (MQ=255)                                              gCCAGCGTTGACTAATATCAACTCTATTTGTTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCATCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCCCATCGAACATCTGGTCGAGGATCTCTGCAAGACTTGTCATACGATCAGTTACTCTGCAAGt                                                                                                                                                                                    <  2:364074/250‑1 (MQ=255)                                                 agagTTGACTAAAATCAACGCTACTTGCTGTAGTGTGTTCTTTCATGCGTCACTGTTCGAGGTAATCCCGCGTCGCCAGCAGTGTGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATATAGAGATGTTGCTGCCATTGATCCATTACCGTTTTGTGATCGTTAAGCACAGCGAACACCTGGTCGAGAATCTCTGCATGACGTGTCATCTGATCAGTTACTCTGCAAGTCTtg                                                                                                                                                                                >  2:247964/4‑251 (MQ=255)                                                          tAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTc                                                                                                                                                                       >  1:634595/1‑251 (MQ=255)                                                            atatCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGt                                                                                                                                                                     >  1:241112/1‑251 (MQ=255)                                                                 aaCGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATc                                                                                                                                                                <  1:193580/251‑1 (MQ=255)                                                                      tATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGCGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGCTCa                                                                                                                                                               >  2:502720/1‑247 (MQ=255)                                                                      tATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCa                                                                                                                                                               <  1:502720/247‑1 (MQ=255)                                                                              gTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCa                                                                                                                                                    >  1:244349/1‑250 (MQ=255)                                                                                aTTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCTTCGGCAAtt                                                                                                                                                 >  2:24398/1‑251 (MQ=255)                                                                                aTTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATATCTGCAAGACGTGTCATACGATCGGTTACTCTGCAGGTCTTGCTGCGCTTCGTTGATAAGCGCCTCGGCAAtt                                                                                                                                                 >  2:620032/1‑251 (MQ=255)                                                                                  ttggTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGc                                                                                                                                                     <  1:1140/243‑1 (MQ=255)                                                                                  tGGGTTCTTTCCTGCGTAACTGTTCGAGGGAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCAATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGCTCTCTGCAAGACGTGTAATACGATCAGTTACTCTGCAAGTCTTGCTGAGCTTCGTTGATCAGCGCATCGGCagttcg                                                                                                                                               >  2:1140/1‑246 (MQ=255)                                                                                     gttcgttcCTGCGTAACTTTTTGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTTCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACAGCTGGTCGAGGATCTCTGCAAGACGTGTCATACGAGCAGTTACTCTGCAAGGCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGcc                                                                                                                                            <  2:642203/251‑1 (MQ=255)                                                                                            ccTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGCTCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCTCTTCGTTGATCAGCGCATAGGCATTTTTGCCGGTGTcc                                                                                                                                     >  2:71618/1‑251 (MQ=255)                                                                                            ccTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTc                                                                                                                                      <  1:24398/250‑1 (MQ=255)                                                                                              tGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTcc                                                                                                                                     >  1:72045/1‑249 (MQ=255)                                                                                              tGCGTAACTGTTCGAGGTAATCCAGCGGCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCTCAGCGGCAATTTTGCCGGTGTcc                                                                                                                                     <  2:72045/249‑1 (MQ=255)                                                                                               gCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCAt                                                                                                                                   <  1:419865/250‑1 (MQ=255)                                                                                                               tAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATACATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGCGGAACTCTGCAAGAAGTGTCATACGATCAGTTAATCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTAACCGttcc                                                                                                                  >  2:351959/1‑249 (MQ=255)                                                                                                                              agcagTGCGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTCCGAATCTCCAGtttt                                                                                                    >  2:66544/1‑250 (MQ=255)                                                                                                                                 agTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTTATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACg                                                                                                <  1:351959/251‑1 (MQ=255)                                                                                                                                 agTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACg                                                                                                <  2:23787/251‑1 (MQ=255)                                                                                                                                 agTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTATTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGAGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACg                                                                                                >  1:331940/1‑251 (MQ=255)                                                                                                                                     aGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTc                                                                                            >  1:269084/1‑251 (MQ=255)                                                                                                                                       cTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGCGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGATATTTTGCCGGTTTCCTTTTTTTGGTCACCGTTACGAATCGCCAGTTTTACCGTTTCgc                                                                                          >  2:13346/1‑250 (MQ=255)                                                                                                                                       cTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTTCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCgc                                                                                          >  2:239846/1‑250 (MQ=255)                                                                                                                                         tttttGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCGTCAAGACTTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCTCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGac                                                                                         <  1:441302/250‑1 (MQ=255)                                                                                                                                            ttGTGCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGAGCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACg                                                                                      <  2:290493/250‑1 (MQ=255)                                                                                                                                                                               tCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACTTTCAAGATTGATATCACTGCTGcc                                                              >  2:499152/1‑239 (MQ=255)                                                                                                                                                                               tCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGcc                                                              <  1:499152/239‑1 (MQ=255)                                                                                                                                                                                   ccccATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGtttt                                              >  1:391536/1‑251 (MQ=255)                                                                                                                                                                                            gagaTGTTGCTACTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGTTATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGcgcg                                     >  2:356423/1‑251 (MQ=255)                                                                                                                                                                                                    gctgctCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTaa                             <  2:244349/251‑1 (MQ=255)                                                                                                                                                                                                    gctgctCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTa                              >  1:374349/1‑250 (MQ=255)                                                                                                                                                                                                       gctCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGt                          <  1:71618/251‑1 (MQ=255)                                                                                                                                                                                                            tGATCCATTACCGTGTGTAGATCGTTAATCACAGCTAACATCTGGTCGAGGAGCGCGGCAAGACGTGTCATACGATCAGTTACTCTGCAAGGCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTTTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGTATCAGTTTTGCTTTCGCGTCGCTTAACGTcaca                      <  2:286882/250‑1 (MQ=255)                                                                                                                                                                                                                ccATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCAct                                                                    <  2:616462/200‑1 (MQ=255)                                                                                                                                                                                                                ccATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCAct                                                                    >  1:616462/1‑200 (MQ=255)                                                                                                                                                                                                                ccATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACActggt                 <  1:350847/251‑1 (MQ=255)                                                                                                                                                                                                                           ttGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAg       <  1:239846/250‑1 (MQ=255)                                                                                                                                                                                                                               gaTCGTTAAGCACAGCGAACATCTGGACGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGCTCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTTATGGTGGAGGCggg  >  2:217491/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGT  >  NC_000913/1129557‑1130029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑