Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,762	G→A	V282V (GTC→GTT)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,762	0	G	A	98.0%	142.9 / NA	50	V282V (GTC→GTT)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (33/15);  total (34/16)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGC  >  NC_000913/1121519‑1122002                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ataCAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGGTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑g                                                                                                                                                                                                                                           <  1:643055/251‑1 (MQ=255)     aGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAAGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGt                                                                                                                                                                                                                                        >  1:94449/1‑250 (MQ=255)         aaaaTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTg                                                                                                                                                                                                                                   <  1:622525/251‑1 (MQ=255)             tAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCACCCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAAACCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGATCTGCAAAGCATTCATTTCTTCAAAAAAGGTA‑GCGTAACTGccac                                                                                                                                                                                                                               >  2:531360/1‑249 (MQ=255)                ccTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCAAgcg                                                                                                                                                                                                                            >  1:584921/1‑251 (MQ=255)                        caacaaGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTggg                                                                                                                                                                                                                    >  1:379574/1‑251 (MQ=255)                               aaGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTg                                                                                                                                                                                                              >  1:589501/1‑250 (MQ=255)                                       aTTTTTATTGTTTAACGGACCATCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGACAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGGA‑GCGTAACAGCCCAGCGCGGTTGGGGCGTTGAagcagcac                                                                                                                                                                                                     >  2:357537/1‑249 (MQ=255)                                       aTTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGccc                                                                                                                                                                                                     >  1:50139/1‑251 (MQ=255)                                          tttatagcttAACGGACCAGCGTACCGTTTCACCGCCGAGGACTGGAACCAGCGTGTCATCAGTCAGTGCGATGCTTTCCGCAACCTGTTGCTCTTCAACTACCCGTTCGATGAATGTGTCGTTGACCGGAAACCCATCGAAATGCGGGCCGTTTACCGAAAAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑ACGTAACTGCCCAGCGCGGTTGGGGCGTTGAagaagaacgac                                                                                                                                                                                                  >  2:567311/9‑242 (MQ=255)                                           ttATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCAATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCcgagc                                                                                                                                                                                                 >  2:663797/1‑248 (MQ=255)                                               tGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGcc                                                                                                                                                                                             >  1:183928/1‑251 (MQ=255)                                               tGTTTAACGCACCAGCGGACCGTTGCCCCGGCGAGGAAGGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGc                                                                                                                                                                                              <  2:589501/250‑1 (MQ=255)                                                 tttAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGc                                                                                                                                                                                           >  1:82013/1‑251 (MQ=255)                                                        gACCAGCGTAACATTTCCCCGGCGAGGCCTGCAACCAGCGGGTCATCGGCCCGTGAGATGCATTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGGCGTTGACCGGCAACCCATAGAACCGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGGACAGACGCAACTGcc                                                                                                                                                                                    >  2:351056/1‑250 (MQ=255)                                                                    gTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATg                                                                                                                                                                        >  1:418712/1‑251 (MQ=255)                                                                       tCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATgtcg                                                                                                                                                                     >  1:97731/1‑248 (MQ=255)                                                                           cGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAA‑TACCGTAAGCGTAACTGCCAAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCAGCAACAGCTCTCTTTGCGGTGACGTGc                                                                                                                                                                  >  2:130753/1‑250 (MQ=255)                                                                             gCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCAATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCAtg                                                                                                                                                               <  1:663797/251‑1 (MQ=255)                                                                                 ggAATGGCACCAGCGTTTCATCAGTCAGTTCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGgc                                                                                                                                                           <  2:359931/251‑1 (MQ=255)                                                                                 ggAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGgc                                                                                                                                                           <  1:87674/251‑1 (MQ=255)                                                                                  gAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGgcg                                                                                                                                                          >  1:551934/1‑251 (MQ=255)                                                                                    aTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGgcgc                                                                                                                                                         >  1:414271/1‑250 (MQ=255)                                                                                                       agtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGCTGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAAt                                                                                                                                      >  1:582613/1‑250 (MQ=255)                                                                                                                                     ctctTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCAc                                                                                                       >  1:595265/1‑251 (MQ=255)                                                                                                                                        ttCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGc                                                                                                     >  1:272353/1‑250 (MQ=255)                                                                                                                                           ccttACCAGTTCGATGAAGGTGCCGTTGCCCGGCAACTCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGGGCTGCAAAGCATTCTTTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGGGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATg                                                                                                 <  2:379574/248‑1 (MQ=255)                                                                                                                                           aCGTACAAGTTCGATGAATGTGTCGTTGACAGGCAACACATAGAACTGCGGGCCGTTTACAGAACAGAATACATAAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGGA‑GCGTAACTACCCAGCGCGGTTGGGGCGTTGAAGAAGCCCGCGCAGCCGCACCTGCTCTCTTTTCGATGCCGTGCATGTGGCGCCGAAACCGTACCGAGGAATACTTGATTAATACCGCGAGCTACCAGTTCACTCAAtt                                                                                                 >  2:11778/1‑250 (MQ=255)                                                                                                                                                  agttcaatgatgcggtCGTTGACCGGCATCCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGAGCTGCAAAGCATTAATGTCTTCAAAAACGGTA‑GCGTAACTGCCCTGCACGGTTGGGGCTTTGAAGCAGACCGTGCAGCCGCAACGGCTCTATTGGCGATAACGTGCATGTGGCGCAGAATCCGTACCGAGGAATTCTCGATTAAAACCGCTGGCGACCAGTTCACGCACTGCCTGTTg                                                                                          <  1:387738/237‑1 (MQ=255)                                                                                                                                                               tgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCAt                                                                                                                                                                <  1:537096/168‑1 (MQ=255)                                                                                                                                                               tgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCAt                                                                                                                                                                >  2:537096/1‑168 (MQ=255)                                                                                                                                                               tgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGa                                                                                                                                >  1:420415/1‑200 (MQ=255)                                                                                                                                                               tgtCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGGAACTGCCCAGCGCGGTTTGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTTCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGa                                                                                                                                <  2:420415/200‑1 (MQ=255)                                                                                                                                                                  cGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTTCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCTCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTg                                                                          <  2:677677/251‑1 (MQ=255)                                                                                                                                                                        ccGGCACCCCATAGAACTGCAGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAACGCGGTTGGGGCGTTGAAGAAACACGCGCAGCCGCAACTGATCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGCGGAATACTCGATTAACACCGCTGGCGACCAATTCCCGCAATGCCTGTTGGTGACTATTACGTTTGCGGATg                                                                    >  2:632361/1‑251 (MQ=255)                                                                                                                                                                           gCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCCTTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGAAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATAATCGATTAAAACCGCGGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTggggtggggt                                                                 >  2:496301/1‑242 (MQ=255)                                                                                                                                                                                      aaCTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGAAGACAATAc                                                        >  1:673265/1‑249 (MQ=255)                                                                                                                                                                                               ccGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGGTTGAGGATGGGTAGACAATACAGGTGCGGacg                                             <  1:351056/251‑1 (MQ=255)                                                                                                                                                                                                   ttACAGAACAGAATGCTTCTAAGGGCTGCAAAGCATTCATTTCTACAAAACCGGTA‑GCGATACTGCCGAGCGCGGTGGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCCCAGAATCCGTACCGAGGAATTCTCGATTAAATCCGCTGGCGACCAGTTCACGCAATGCCGGTTGGTGAATATTACGTTTGAGGATGCGTTGACAATACATGTGCGGacgctc                                          <  2:8717/250‑3 (MQ=255)                                                                                                                                                                                                         aacagaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGCTTAAAACCGCTGGCGCCCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGACTcc                                    >  1:336217/1‑250 (MQ=255)                                                                                                                                                                                                            agaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTa                                                                                                                             <  2:504389/158‑1 (MQ=255)                                                                                                                                                                                                            agaaTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTa                                                                                                                             >  1:504389/1‑158 (MQ=255)                                                                                                                                                                                                            agaaTGATTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTGTGCGATGACGTGCATGTGGCGCAGAAGCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGCCCAGTTCACGCGATGCCTGTTGGTGCATATTAAGTTTGAGGATGGGTAGACAATACAGGTGCGGTAGCAAGCCTCCAAcc                                >  2:516868/1‑251 (MQ=255)                                                                                                                                                                                                               aTGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAACAGCCCGCGCAGCCGCACCTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGc                             >  2:154050/1‑251 (MQ=255)                                                                                                                                                                                                                   ttCAAAATGCTGCAAAGCATTCATTTCTTCAACAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGCATACTCGATTAAAACCGCTGGCGACCAGATCACGAAATGCCTGTTGTGGAATATTACTGTTGAGGATGGGTAGACAATACAGGTGCGAGCGAACGCCTCCAACCAGAatat                         >  2:514921/1‑251 (MQ=255)                                                                                                                                                                                                                       aaGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGAGAATATTACGTTTGCGGATGGGTAGACAATACAGGTGCGGAAGCACACCTCCAACCAGCATATGGtt                     >  2:11688/1‑251 (MQ=255)                                                                                                                                                                                                                        aGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGTGGCGTTGAAGCAGCCCGCGCAGCAGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACAGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTg                    >  2:287749/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaGCATTCATTTCTTCAAAAACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCATGTTGGGGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGAAGCACGCCTCCAACCAGCATATGGTTGCGGTTAAAc           >  2:458038/1‑251 (MQ=255)                                                                                                                                                                                                                                         attgttttataGACGGTA‑GAGTAACTGTCAAGTGTGGCTGGGTCGTTGAAGAAGCACGTGAAGCCGCAACTGCTCTCGTTGCGATGAAGTGAATGTGGCGCAGAATTCGTACCGAGGAATACTCGTTTAAATCCGCTGGCGACCAGTTCACGTAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGATAATACAGGTGCGGACGAATGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtg   <  1:150368/241‑1 (MQ=255)                                                                                                                                                                                                                                           ttcttcAAAACCGGTC‑GTGTACCTGCCGAGCGCTGTTGGGGCGTTGAAGCAGCCCGAGCATCCGCAACTGCTCTCTTTTCGATGACGTGCATGGTGCGCATAATCCGTTCCGAGGAATCCCCGATTAAAACCGCTGGCGACCAGTTCTCGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCTTATGGTTGCGTTTAAACATCAGAtgc  <  2:582613/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTA‑GCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGC  >  NC_000913/1121519‑1122002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑