Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,547	G→A	intergenic (‑60/+14)	dinI ← / ← pyrC	DNA damage‑inducible protein I/dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,547	0	G	A	100.0%	92.9 / NA	29	intergenic (‑60/+14)	dinI/pyrC	DNA damage‑inducible protein I/dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/12);  total (17/12)

TATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGG‑GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGG  >  NC_000913/1121310‑1121792                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       tATCTTCTTTTGTCGCGCCAATAACCGATACATTATTCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGAGAAA‑TGCGCCCGCCCGGGGGTCAATAACCCCAGCCTGCAATGGGGAAGTTTTTGCTATTGGGGCTTCAATTCGCATAATAACCCCCCGTTTGATTTTCTTGTTATTTATACAGGAAAAAAAACCTAATGACAACAAAAAGATACGAttt                                                                                                                                                                                                                                           >  1:180549/1‑251 (MQ=255)          gttgcgcgCCAATAACCTATAAATTAATCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGTAATCGCATACTGAATACGGCGG‑GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACTGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGttt                                                                                                                                                                                                                                  <  2:367669/247‑1 (MQ=255)                               aaTTATTCGCTGCGGCATAACGTACCGATCCGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGG‑GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGTCGTCAATTCGCATAATAGCCCTCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTGCGATTTTTATTGTTTAACGGACCAGCGTACCGTTTc                                                                                                                                                                                                             <  1:556204/251‑1 (MQ=255)                                                                                               aCGGCGG‑GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGctc                                                                                                                                              <  1:676055/250‑1 (MQ=255)                                                                                                   cgg‑GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCAc                                                                                                                                         >  1:531236/1‑251 (MQ=255)                                                                                                    gg‑gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCGGCAACCTGTTGCTCTTCACg                                                                                                                                        >  2:33278/1‑251 (MQ=255)                                                                                                               gccagccaGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCg                                                                                                                              <  1:471899/251‑1 (MQ=255)                                                                                                                      agGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAAtg                                                                                                                       >  1:222600/1‑251 (MQ=255)                                                                                                                               aTAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGa                                                                                                             <  1:618049/251‑1 (MQ=255)                                                                                                                                  gCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTTAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCg                                                                                                          <  2:611350/251‑1 (MQ=255)                                                                                                                                         cTGGCACTGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATACTAGCCCACTGTTGAATATACTGGTTCTTTATACAGTTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCCCCAGCGTGTCATAAGTCAGTGCGATGCTTTAAGCAACCTGTTGCTCTTCCCATACCAGTTCGATGAA‑GTGGTCGTTGCCCGGCAAccc                                                                                                   >  2:614973/1‑251 (MQ=255)                                                                                                                                           ggCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCa                                                                                                  <  1:33278/250‑1 (MQ=255)                                                                                                                                                         tttCGCTATGGTGACTTCAATTCGCTTAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGACCg                                                                                   >  2:201362/1‑251 (MQ=255)                                                                                                                                                                      aCTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaac                                                                         >  1:408334/1‑248 (MQ=255)                                                                                                                                                                               cGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCa                                                              >  1:156180/1‑250 (MQ=255)                                                                                                                                                                                      taGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGc                                                                                         >  1:329846/1‑216 (MQ=255)                                                                                                                                                                                      taGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGtgctg                                                      >  1:464056/1‑251 (MQ=255)                                                                                                                                                                                      taGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGAGTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGc                                                                                         <  2:329846/216‑1 (MQ=255)                                                                                                                                                                                         cccccTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGACTGTG‑TCGTTGACCGGCAACACATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCa                                                    >  2:568069/1‑250 (MQ=255)                                                                                                                                                                                                         cTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATttcttc                                    >  1:359931/1‑250 (MQ=255)                                                                                                                                                                                                                   ataCAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGGTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAg                         <  1:643055/251‑1 (MQ=255)                                                                                                                                                                                                                       aGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAAGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGt                      >  1:94449/1‑250 (MQ=255)                                                                                                                                                                                                                           aaaaTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTg                 <  1:622525/251‑1 (MQ=255)                                                                                                                                                                                                                              aTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCg                                                                                                                                                                     <  2:23669/101‑1 (MQ=255)                                                                                                                                                                                                                              aTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCg                                                                                                                                                                     >  1:23669/1‑101 (MQ=255)                                                                                                                                                                                                                               tAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCACCCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAAACCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGATCTGCAAAGCATTCATTTCTTCAAAAAAGGTAGCGTAACTGccac             >  2:531360/1‑249 (MQ=255)                                                                                                                                                                                                                                 aCCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCaaaaa                               <  1:113853/231‑3 (MQ=255)                                                                                                                                                                                                                                 aCCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCaaaaa                               >  2:113853/1‑229 (MQ=255)                                                                                                                                                                                                                                  ccTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCAAgcg          >  1:584921/1‑251 (MQ=255)                                                                                                                                                                                                                                          caacaaGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTggg  >  1:379574/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       TATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGG‑GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTG‑TCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGG  >  NC_000913/1121310‑1121792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑