Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,389	C→T	A368T (GCA→ACA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,389	0	C	T	96.2%	158.7 / ‑4.5	53	A368T (GCA→ACA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (21/29);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTGTAATCA  >  NC_000913/1114161‑1114619                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        aaGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACg                                                                                                                                                                                                                   <  2:54939/251‑1 (MQ=255)    cGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATATCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACTACGTCGTAGACCGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATTCCGCTCTTAAACCGTAGTTCGCTTAAATCGCTGTTCCCATc                                                                                                                                                                                                                                  <  1:470693/233‑1 (MQ=255)                   catcaCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAAc                                                                                                                                                                                                 <  1:227366/251‑1 (MQ=255)                       aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAtt                                                                                                                                                                                             >  1:402094/1‑251 (MQ=255)                                 cTTTGAGTTGATGAGGTTTCAGGGAAACGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGtt                                                                                                                                                                                                                 >  2:430711/1‑221 (MQ=255)                                 cTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGtt                                                                                                                                                                                                                 <  1:430711/221‑1 (MQ=255)                                         ttagtatgttttctgaaaaaGGATATCTTATTCTCCTGTTTTGCAAGGATGAAAGGCGAAAACTCAGTTCGATACCTGGTGTATTCGACGACGGCGTAGAATGTTCCATGAATAGTCTGCGTTGAATAACACTACGCCTGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTTCTATCAATGGTCTGGTAACGTTGCCAATTTCTCGAAACGAGTGGTTATTGCTGAAtata                                                                                                                                                                            <  2:629229/234‑1 (MQ=255)                                         tgaggtggTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGAAGTAGACTGTTCCATGAATAGACTTCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGGTATAGCTGAAGATa                                                                                                                                                                            <  2:587790/244‑1 (MQ=255)                                             gaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGcc                                                                                                                                                                        <  1:112399/250‑1 (MQ=255)                                             gaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACAGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGccc                                                                                                                                                                       <  1:514690/251‑1 (MQ=255)                                                     agtgAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGGTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAATATACGCCCGGCGATCt                                                                                                                                                               <  1:198974/248‑1 (MQ=255)                                                                      aTTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑Ta                                                                                                                                              >  2:144544/1‑250 (MQ=255)                                                                        tctcCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAAc                                                                                                                                           >  1:248889/1‑251 (MQ=255)                                                                           ccGCTTTTGCAAGTATGAAAGGTGAAAAAGCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATTAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAAcc                                                                                                                                          <  1:623007/249‑1 (MQ=255)                                                                           ccGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAAcc                                                                                                                                          <  1:144544/249‑1 (MQ=255)                                                                             gCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGCTTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAAc                                                                                                                                       >  2:261138/1‑250 (MQ=255)                                                                                    cAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTg                                                                                                                               >  1:607494/1‑251 (MQ=255)                                                                                          tGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACAGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGACCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGAGTCTGTAcggc                                                                                                                         >  1:532766/1‑251 (MQ=255)                                                                                           gAAAGGCGAAAAATCAGTTAGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGTCGATCTGGTTGCGCGAGTTG‑TAAACCAACAGTGTCTGTAcggcg                                                                                                                        >  2:138117/1‑251 (MQ=255)                                                                                               ggCGAAAAATCAGTTCGATACCTGGGGGATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGa                                                                                                                    <  2:219328/251‑1 (MQ=255)                                                                                                  gAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGagta                                                                                                                 <  1:226932/251‑1 (MQ=255)                                                                                                      aaTCAGTTCGATTCCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTTAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTGCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCTCGAAACGATTGTTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGc                                                                                                             <  1:652767/251‑1 (MQ=255)                                                                                                            ttCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGGGTAGTGCACCATc                                                                                                       >  1:458411/1‑251 (MQ=255)                                                                                                              cGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAAAAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATcg                                                                                                      <  2:607494/250‑1 (MQ=255)                                                                                                                 tACCTGGGGTATTCGACGACAACGTAGACTGAACCATGAATAGCCTGCGTTGAATAACACAACGCCAGCTGTGACGAGAAATACCGCTCTGACACAGTAGTTCGCAGAAATAGCAGTTCCCATAAATGGTCCGGTAACGTTGCCAATATCACGAAACGCTTGGTTATAGCTGAAGATACGCACGGCGATCTGGTAGCTCGAGTTG‑TAAACCCACAGGGTCTGTACGGCGGGGagatgtggaacctcgcggg                                                                                                  >  2:639954/1‑234 (MQ=255)                                                                                                                  aCCTGGGGTATTCGACGACGACGTAGAATGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCggcggc                                                                                                 <  1:59375/251‑1 (MQ=255)                                                                                                                     tGGGGTATTCGACGCCGACGTAGACGGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCACTGGTCCGGTACCGTTGCCAATATCACGAAACGATTGCTTATAGCTGACGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCACCAGTGTCTGTACGGCGGGGTGTCTTGCACCacagcggccgcc                                                                                              >  2:529667/1‑240 (MQ=255)                                                                                                                           aTTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAAGCGCTGTTCCCATCAATTGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCTCCGAGCa                                                                                         <  1:371605/250‑1 (MQ=255)                                                                                                                                 cgacgacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACAAACAGGGTCTGTACGGCGGGGAGTAGTGCACCACTGGCGGCCCCCAGCaaaaaa                                                                                    >  2:96051/1‑249 (MQ=255)                                                                                                                                       cgTAGACTGTTCCATGAATAGACTGCGTTGAATACCACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCaaaaaaaggtaaa                                                                             >  1:160513/1‑243 (MQ=255)                                                                                                                                        gTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATc                                                                           <  2:402094/251‑1 (MQ=255)                                                                                                                                          aGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATcc                                                                          <  1:33260/250‑1 (MQ=255)                                                                                                                                                      gAATCGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTACCGTTGCCAATATAACGAAAGGTTTGGTTATGGCGGAGCAAAGGCCCGCCGTCCGGGTCGC‑CCAGTTGGAAAACCAAAAGTTTTTGTACGGTGGGGAGTAGGGAACCAGAGGCGGGACCGAGAAAAAAAAGTAAAAACCCAAgaaggggaa                                                              >  2:535624/1‑242 (MQ=255)                                                                                                                                                      gAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATg                                                              <  1:83336/250‑1 (MQ=255)                                                                                                                                                      gAATAGACTGCGTTGAATAACACTACGCCAGCGGAGACGAGAAATACCGCTCTGAAACAGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATATGGTTGCTCGAGTTG‑TAAAACAACAGTGTCTGTACGGCGGGGAGAAGTGCACCAACGGCGGCACCGAGCAAAAAACGGAAAATACCAAGTTGCAATgg                                                             >  1:152788/1‑251 (MQ=255)                                                                                                                                                             cggcggtGAATAACACTACGCCAGCGGTGACGAGAAATACCGCGCTGAAACCGTAGTTCGCTGAAAACGCTGGTCCCATCAATGGTCCGGTAACGTTGCTGATATCCCGAAACGATTGGTTATAGCTGAAGATACGCCCAGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGa                                                      <  2:189532/245‑1 (MQ=255)                                                                                                                                                                 gTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACgg                                                  >  1:389455/1‑250 (MQ=255)                                                                                                                                                                    gaagaacccgaCGCTAGCGGTGACGAGAAATACCGCGCTGAAACCGTAGTGCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAGCCATCAGTGTCTGTACGGCGGGGAGTAGTGCATCGGCGGCGTCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCAGAACGTAAg                                               <  1:296467/241‑1 (MQ=255)                                                                                                                                                                    gAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGCGTAGTGCACCATCGGCGGCCCCGAGCAAAAAAAGGAAAATCCCAAGTTGCAAAGGCGTCTGAACGTaa                                                >  1:570510/1‑250 (MQ=255)                                                                                                                                                                     aaTAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGa                                              <  1:210176/251‑1 (MQ=255)                                                                                                                                                                      agaaCACTACGCCAGCGGGGACGAGAAATACCGCTCTTAAACCGTAGTTCGCTGAAATCGCTGTTCCCAGCAATGGTCCGGTAACGTTGCCAATAGCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTTGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGAc                                             <  2:393576/249‑1 (MQ=255)                                                                                                                                                                         acacTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACAtt                                          <  1:408681/251‑1 (MQ=255)                                                                                                                                                                          cacTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTg                                         >  1:214128/1‑251 (MQ=255)                                                                                                                                                                                gCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGGAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGGGTAGTGCACCATCGGCGGCACCGAGGAAAAAACGTAAAATCCCAAGTTGCAAAGGCGGCTGAACGTAAGACATTggagtc                                    >  1:50633/1‑246 (MQ=255)                                                                                                                                                                                 ccAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGGGCAAAAAACGTAAAAACCCAAGTTGCACAGGCGTCTGAACGTAAGACATTGGGATcc                                   >  1:501628/1‑249 (MQ=255)                                                                                                                                                                                    gCGGTGACGAGAAAGACCGCTCTGAAACCGTAGTGCGTGGAAATCGCTGTTCCCATCAATGGTACGGTAACGTGTCCAATATCACGAAACGAGTGGTTATAGCTGAAGATACGCCCTGCTATCTGGTTGCGCGAGTTG‑GAAACCAACAGTGTCTGTACGGCGGGGAGTAGGGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACAGTGGGATCAAcag                               <  2:75421/251‑1 (MQ=255)                                                                                                                                                                                         gACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTa                          <  2:573872/251‑1 (MQ=255)                                                                                                                                                                                          aCGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAATGCGCTGTTCCCAACAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTAc                         <  2:281076/251‑1 (MQ=255)                                                                                                                                                                                              gaAATACCGCTCGGAAACCGTAGTTCGCTGAAATCTCTGTTCCCATCAATGGTCCGGTAACGGTGCCAATATCACGAAACGATTGGTGATAGCTGAAGATACGCCCGGCGATCTGGTGGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGGAGTGCACCATCGGCGGCACCGAGAAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGaa                     <  2:662594/251‑1 (MQ=255)                                                                                                                                                                                                          tGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGGTCAACAGCAGTACAGAAAAGATCAGCGCt         >  1:514698/1‑251 (MQ=255)                                                                                                                                                                                                           gAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTg        >  1:615957/1‑251 (MQ=255)                                                                                                                                                                                                               ccGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATcc                                                                          <  1:646154/181‑1 (MQ=255)                                                                                                                                                                                                                 gTAGTTCGCTGAAATCGCTATTCCCAACAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTGTAATCa  <  1:527931/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        AAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTG‑TAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTGTAATCA  >  NC_000913/1114161‑1114619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑