Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,421	C→T	S520F (TCT→TTT)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,421	0	C	T	100.0%	173.6 / NA	55	S520F (TCT→TTT)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/22);  total (33/22)

GGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGG  >  NC_000913/1112177‑1112665                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ggTTCCTTTGCCGGTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTAtct                                                                                                                                                                                                                                                >  1:299713/1‑251 (MQ=255)      cTTTGACGGTTCAATCCCATCAAATGATTTCGTGGAAGCGGCGTTTATGCGCAGTGCACGTTGGGGGATCTTGATTGCTTACGATCGCCCGGGTTCTTATGACGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGCGCCACGGTAACCTGATGTCCTTCCGTCTGTTCCTAGTGACGGTAATGCAACCGGTTCACCGTGCGTAGTTCATGCAGGGCGTGATGTTTTATCTctccg                                                                                                                                                                                                                                           >  2:85107/1‑251 (MQ=255)         tGCCGGTTCAATCCTGTCACCTGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGCGCTAAAACGTGCCCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGCGAAGGGTATGCACCCGGTTCACCGGGCGGGGTTCCTGACGGGCGTGATGTTTAATCTctccgctc                                                                                                                                                                                                                                        >  2:161424/1‑251 (MQ=255)                  aacccTGTCACATGATTTGGTGGAAGCGGCGTTGAGGCGCCGTGCAGGTGGGGGGGTCTGGATTGCTTACGATCCCCCGGGTTTTTATGAAGAATTCCCGCCAAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGt                                                                                                                                                                                                                               <  1:104039/248‑1 (MQ=255)                        gTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATg                                                                                                                                                                                                                          >  1:318631/1‑250 (MQ=255)                        gTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGt                                                                                                                                                                                                                         >  1:189006/1‑251 (MQ=255)                             aTGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTc                                                                                                                                                                                                                    >  1:492822/1‑251 (MQ=255)                              tggttttggggaggcggggTTGAGGCGCCGGGGGGGTGGGGGGGTGTGGATGGCTTACGATCTCCGGGTTTCTTAGGAAAAAGTGCCGCCAATCTTTCTTGATGACGTAAACCGTGTCCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTc                                                                                                                                                                                                                    <  1:84065/237‑1 (MQ=255)                                 tttCGGGGAGGCGGCGGTGATGCGCCGTGCAGGTGGGGGGGTCTGTATTCCTTACGATCCCCGGGGTTTTTAAGAAGAATGGCCGCCAAACTTGCTGGTGGAGCAAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTcgcg                                                                                                                                                                                                                 <  1:162797/249‑1 (MQ=255)                                         aaggggtgTTGATGCCCGGTGCAGGTGGGGGGGTGTGGATTGTTTACGATCTCCCGGGTTATAATGAAGAATTGCCGCCTAACTTGCTTGATGGGCAAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACt                                                                                                                                                                                                        <  1:641440/244‑1 (MQ=255)                                             ggagTTGATGCGCCGTGCAGGTTGGGGGGGCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGACTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTACCCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGATTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCAt                                                                                                                                                                                                    >  1:285510/4‑251 (MQ=255)                                                gTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGc                                                                                                                                                                                                 >  1:437600/1‑251 (MQ=255)                                                            tGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCTCTCTCTACTGCATTGCAGGTCGTGCATg                                                                                                                                                                                     >  1:552041/1‑251 (MQ=255)                                                             gCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGt                                                                                                                                                                                          >  1:628249/1‑245 (MQ=255)                                                                    gggggtTCTGGATTGCTTACGATCTCCCGGTTTTTTAGGAAGAATCGCCGCCTAACTTTCTTGATGAGCAAAAACGTGCCCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGAcc                                                                                                                                                                             <  1:137386/251‑1 (MQ=255)                                                                    ggggggTTGGGATTGTTTACGAGCCCCCGGGTTCTAAGAAAGAATTGCCTCCTAATTTGTTTGAGTAGCTAAACCGTGCCCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGAcc                                                                                                                                                                             <  1:501693/251‑1 (MQ=255)                                                                                  gCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTAt                                                                                                                                                                                                                           >  1:250259/1‑191 (MQ=255)                                                                                          tctcCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAAc                                                                                                                                                       >  1:421473/1‑251 (MQ=255)                                                                                            ccccGGGTTCTTAGGAAGAATTGCCGCCTAACTTGCTTAATGAGCTAAACCGGGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCa                                                                                                                                                     <  1:107824/250‑1 (MQ=255)                                                                                                       tATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAgttgtt                                                                                                                                          >  1:349037/1‑251 (MQ=255)                                                                                                               aTTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACAGCAATACTTCCTGCAACCACGGCCGTTGTTCCCAgtgt                                                                                                                                  >  2:293142/1‑251 (MQ=255)                                                                                                                  gccgccTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCTGTGTGGc                                                                                                                               >  2:639634/1‑251 (MQ=255)                                                                                                                    cgccTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGAATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCg                                                                                                                             >  1:20937/1‑251 (MQ=255)                                                                                                                      ccTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGc                                                                                                                            >  1:372527/1‑250 (MQ=255)                                                                                                                                 tatggagcaaaaaggggACCGGCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCTTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTGTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCGCTCTACTGCATTGCAGGTAGGGCATGCGTTGACCGAGCCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCt                                                                                                                 <  1:303139/234‑1 (MQ=255)                                                                                                                                  gatgaGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCt                                                                                                                                                            >  1:2071/1‑206 (MQ=255)                                                                                                                                       gCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTgg                                                                                                          >  1:25333/1‑251 (MQ=255)                                                                                                                                         tAAAACATGACCGCAGCTGGTGCCCCGGTAACCTGATGAACTTCAGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCCCCGTGCGGCGTTCCTGACGGGCGTGCTGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGCCCGAACCGCAATACTTCCTGCAACCACGGCAGTTTTTCCCGGTGTGGCCGCAGTGGCGTCCTGAGCTGGCg                                                                                                        >  2:258471/1‑251 (MQ=255)                                                                                                                                                tGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGGGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCAc                                                                                                 >  1:491237/1‑251 (MQ=255)                                                                                                                                                    cgccgATGTGGCCACGTTAACCTGATGAACTTCCGGCTGTTCCTGGTGAAGGGTATGCCACCGGTTCCCCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGGTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCTCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACtttt                                                                                             <  2:503372/251‑1 (MQ=255)                                                                                                                                                     gccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTTTGGTTTATGTTCCTCGCGCTCTCTACTTCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACttttt                                                                                            <  1:410224/251‑1 (MQ=255)                                                                                                                                                      ccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCACTACTTCCTGCAACCACGGCAGTTGTTCCCCGTGTGGCCGCAGTTGCGTCCTGATCTGGCGATTGCACTTTTTg                                                                                           >  2:271456/1‑251 (MQ=255)                                                                                                                                                       cgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGc                                                                                          >  1:36640/1‑251 (MQ=255)                                                                                                                                                               ccacgttaacctgtttagtgcccGTCTCTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCGGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACGGCAGTGCAGTTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCa                                                                                  <  2:458880/230‑1 (MQ=255)                                                                                                                                                                   gttAACCTGATGAACTTCCGTCTGTTCCGGGTGAAGGGTATGCATCCGGTTCACCGTGCTGTGTTCCTGACGGGCGTGATGTTTTATCTCTACGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTTCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTGCCTGCAACCACTGCAGGTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTTCTTCGACCATGGt                                                                              <  2:552041/249‑1 (MQ=255)                                                                                                                                                                   ggTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATggg                                                                              >  1:12457/1‑250 (MQ=255)                                                                                                                                                                       aCCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCtg                                                                          >  1:427127/1‑251 (MQ=255)                                                                                                                                                                         cTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCtgtt                                                                        >  1:211976/1‑251 (MQ=255)                                                                                                                                                                           gatgaACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCtgttgg                                                                      >  2:522536/1‑250 (MQ=255)                                                                                                                                                                                aCTTCCGTCTTTTCGTGTTGAATGGTAGGCACCCGGTGTACCGTGCGGTGTGCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCACTGGGGTTTATGTTCCTCGCGCTCTCTACTGCCTTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTCCCTGCAGCCACGGCAGTTGTTCCCAGTGTGGTCTCAGTGGCTTCCTGAGCGGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTg                                                                 <  2:383835/251‑1 (MQ=255)                                                                                                                                                                                aCTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTg                                                                 <  1:77673/251‑1 (MQ=255)                                                                                                                                                                                     cGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGa                                                             >  1:1859/1‑250 (MQ=255)                                                                                                                                                                                       tCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGt                                                          >  1:310607/1‑251 (MQ=255)                                                                                                                                                                                          gtttctggggaagtttattcccccGGTTCTGCGTGGGGTGTTCCTGTGGGGCGTGATTTTTTATCTCTCCTCTCCGTTGTGGGTTATTTTCCTCGCGCTCTCTTCTGTATTGCAGGTAGTGCATGAGTTGACCGAACCTCAATACTTCCTGCAACCACGGCAGTGGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGAACTTTTTGCTTCGACCATGGTGCTGTTGTACCTGCCGTAGTTAt                                                       <  2:373439/230‑1 (MQ=255)                                                                                                                                                                                          ggtcctggtgacgtgttggccccgggTTCACCGTGGGTTGTTCCTGACGGGCGTGATGGTTTATCTCTCCGCCCTGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCCTGCGTTGACCGAACCGCAATACTTCCTGCAACCGCGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGGCCTGAGCTGGCGATGGCACTTTTTGCTTCGACCATGGTGCTTTTGTTCCTGCCGAAGt                                                          <  2:518523/224‑1 (MQ=255)                                                                                                                                                                                          gTTCCTGGTGAAGGGTATGCACCCGGTTCCCCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTa                                                        <  1:207201/250‑1 (MQ=255)                                                                                                                                                                                             ccgggTGAATGGTATGCCCGTGGTGCACCGTGCGGTGCTCCTGACGGGCGTGATGTTTTAGCTCTCCGCTCCGCTGTGGTTTATGTTCCGCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCTCTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTg                                                     <  2:299713/247‑1 (MQ=255)                                                                                                                                                                                                  tGAAGGGAATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTACGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGCACCGCACTCCTTCCTGCAACCACGACAGTTGTTCCCAGTGTGGACGCAGTGGCGTCCTGCGCTGGCGATTGCACTTTTTGCTTCGCCCATGGTGCTGTTGTTCCTGCCGAAGTTATTGCGCAtt                                               >  2:333161/1‑251 (MQ=255)                                                                                                                                                                                                   gAAGGGTTTGCACCCGGTTCACCGGGCGGGGTTCCTGACGGGCGTGATGTTTTATCCTTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCAGGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTGGTGCCTGCCGAAGTTATTGATCAttt                                              <  2:427127/251‑1 (MQ=255)                                                                                                                                                                                                   gAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAttt                                              >  1:237584/1‑251 (MQ=255)                                                                                                                                                                                                          aTGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTAt                                       <  1:170716/251‑1 (MQ=255)                                                                                                                                                                                                              aCCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGACCCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGGTGTTCCTGCCGAAGTTATTGATCATTTTGCTTATCTgg                                   <  1:513098/251‑1 (MQ=255)                                                                                                                                                                                                                ccGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGGTGTTCCTGCCGAAGTTATTGAGCATTGTGCTTATCTGGTg                                 <  1:205741/251‑1 (MQ=255)                                                                                                                                                                                                                           tGCGGTGATCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTTCTTATCTGGTGCAAAGGAACg                       >  2:541899/1‑250 (MQ=255)                                                                                                                                                                                                                                               tGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTgg  >  1:271724/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGG  >  NC_000913/1112177‑1112665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑