Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,740	C→T	L136F (CTT→TTT)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,740	0	C	T	100.0%	91.1 / NA	31	L136F (CTT→TTT)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/12);  total (19/12)

ATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACA  >  NC_000913/1109510‑1109979                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 aTCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCaa                                                                                                                                                                                                                               <  1:11631/250‑1 (MQ=255)              gTTTAATTATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATg                                                                                                                                                                                                                 <  2:521919/251‑1 (MQ=255)                    tCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATCCGGTAAAAGACCGTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGCTCAAACGCAAAGATAAAAACGATGAAATCg                                                                                                                                                                                                           >  2:493293/1‑251 (MQ=255)                          cAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGTGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTCCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCCCTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGCCCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGAAAAGATAAACACGATGCAATCGTCAga                                                                                                                                                                                                      >  1:431276/1‑249 (MQ=255)                                       aaCAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAACGATAAAAACGATGAAATCGTCAGCATGCTCGGGGcc                                                                                                                                                                                          >  2:20482/1‑249 (MQ=255)                                                             accTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGtacg                                                                                                                                                                 >  2:351489/3‑248 (MQ=255)                                                                 cggtttCTACCATCATGGTAGGTACTTTGATACCCCGGTCAAAAGATAGGTAGTGACGGCCTCCGCAGTCAATCGAATCAAATACAGTCCGGATTATTTCTCTTTCGGCGTTGTTCAGCATGACAAAGATACGGTAAAAGTCCTTTGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGTTCGGGGCCATCTATTTCCGCGTG‑ATTGGTGcaggt                                                                                                                                                              <  2:401163/246‑1 (MQ=255)                                                                 cGAGTTCTACCAGAAGGGTATGTACATCGATACCCCGGTCAAAAGAAATGAAGTGATCGTCACCGCAGTCAAACGAATCAAATACAGCTCGGAGTATTTCCCTATCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCATGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAATATAAAAACGATGAAATCGGCAGCATGCTCTGGGCCAGCTATTTCCGCGTT‑AGTGGTGcagg                                                                                                                                                               <  2:298910/249‑1 (MQ=255)                                                                      tCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATAGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGtt                                                                                                                                                        >  2:581328/1‑251 (MQ=255)                                                                                gggTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGCCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCttt                                                                                                                                              >  2:339318/1‑251 (MQ=255)                                                                                      gTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGcc                                                                                                                                         <  1:632658/250‑1 (MQ=255)                                                                                        aCTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCcgc                                                                                                                                      >  1:251364/1‑251 (MQ=255)                                                                                                   ccGGTCAAACTAAATGAAGTGGATGCCACAGCATTCAAACGAACCAACTACAGAACGGATTATTTCACTTTCGGAGATGTTCAGCATGACAAATAGACGGTAAAAGACCTTGGCTTTGCCGGTGTTAAAGTGTTTAACCAGATCAACAGCAAAGACAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATCTCCG‑GTGGATTGGGTCAGGAATAGTTAATGGCCATTCTGCCAGCGGCCTGGCAAt                                                                                                                           >  2:39437/1‑251 (MQ=255)                                                                                                        cAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCTATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCAt                                                                                                                                                                                                    <  2:141455/174‑1 (MQ=255)                                                                                                        cAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCAt                                                                                                                                                                                                    >  1:141455/1‑174 (MQ=255)                                                                                                         aaaaTAAATGAAGTGACTGCCACCGCAGTCAAACGAATCTAATACAGCACGGCTTATTTCACTTTCGGCGATATTCAGCATGACAAAAATACGGTAAAAGTCCTTGTCTTTGCCGGTTTTAAAGGGTTTTAACCGCTCAACAGAAAAGATAAAAACGATGCAATCGTCCGCATGCTCGGGGCCAGCTATTTAAGCGTG‑ATTGGTGCAGGTCAGGTTTATGTCATTTCTGCCCGCGGCATGGCCATTGaatc                                                                                                                     >  2:247193/1‑248 (MQ=255)                                                                                                              aaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAAGACAGCCCGGATTATTTCACTTTCGGCGATGTTCATCATGACAAAGATACGGTAAAAGACCTTGGCGTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGTCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCt                                                                                                                <  2:36827/251‑1 (MQ=255)                                                                                                                                   aGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTc                                                                                           <  1:20482/251‑1 (MQ=255)                                                                                                                                      cAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGTCTTGCCATCGGGTGAAGAATTTCcgc                                                                                        >  1:671290/1‑251 (MQ=255)                                                                                                                                        aaCGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTAGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCgcgc                                                                                      >  1:492208/1‑251 (MQ=255)                                                                                                                                             aTCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGAGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGATGACGAATTTCCGCGCTTCa                                                                                  >  2:487171/1‑250 (MQ=255)                                                                                                                                               cAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCaa                                                                                 >  1:359501/1‑249 (MQ=255)                                                                                                                                                    aCAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCAAGCTATTTCCGCGTG‑ATTGGTGAAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTc                                                                          >  1:625584/1‑251 (MQ=255)                                                                                                                                                                  ttCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAATACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGAGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCAATCGGGGGAAGAATTTCCCCGCTTCAAAGAGTTCTGGATCGAGCGTcc                                                            >  2:218629/1‑251 (MQ=255)                                                                                                                                                                          cGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCg                                                     >  1:503941/1‑250 (MQ=255)                                                                                                                                                                               atgtaaaGCATGACAAAGATACGGTAAAAGACCTTGGCTATGCCGGTGTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGAt                                               <  2:573717/245‑1 (MQ=255)                                                                                                                                                                                       cATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGttt                                       >  1:514864/1‑251 (MQ=255)                                                                                                                                                                                                gATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCt                               >  1:290189/1‑250 (MQ=255)                                                                                                                                                                                                 aTACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGGGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCAGGCTCTGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCTAGCGTCCAAAACCGACTGATAAACGTTTAATCATCTAt                             <  2:625011/251‑1 (MQ=255)                                                                                                                                                                                                                         ccGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCg     <  1:80042/251‑1 (MQ=255)                                                                                                                                                                                                                            gTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTAGGCATTGCTTGACTCGCCGCGTGCGACa  <  1:493293/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTG‑ATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACA  >  NC_000913/1109510‑1109979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑