Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,508	C→T	E594E (GAG→GAA)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,508	0	C	T	100.0%	131.6 / NA	42	E594E (GAG→GAA)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/12);  total (30/12)

CGCTGGTTACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCA  >  NC_000913/1090263‑1090739                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         cGCTGGTTACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGc                                                                                                                                                                                                                                     <  1:231987/251‑1 (MQ=255)                      tcattgaacgccaaagagaaccggTACTTACGCCGAACATTTTGAAACAAACGAACATAGGACTGAGCACTGTTGCCTGTAACAACATTTTTCATTGACCGTAATGGAACGCGGAGAGAGAGACGATAAATGTGTGAACCAATACGCCAGTTGTCATTAAAATCATACAAGCCGGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACACGTTCAGCGAATTATGCATCGAACCAGATTTTACGTg                                                                                                                                                                                                                 >  2:650965/24‑250 (MQ=255)                         gTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCg                                                                                                                                                                                                             <  1:389049/251‑1 (MQ=255)                                 ccAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCa                                                                                                                                                                                                      >  2:304737/1‑250 (MQ=255)                                   aGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAac                                                                                                                                                                                                    >  1:413253/1‑250 (MQ=255)                                         cacCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGc                                                                                                                                                                                              >  1:451475/1‑250 (MQ=255)                                                        gcTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACAAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGAATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAACCCAGAcgcacac                                                                                                                                                                               >  2:500411/1‑246 (MQ=255)                                                               ttgatccaaGCGAACATAAGCCGGAGCACTGTTCCCGGTAACACCATTTTTCTTTGCCGGTAAGGGAACGCGGTGAGAGGGGCTTCCCAGTTGCGACCCAACACGCAATTTATCATAAAAATCATCCCAGCCAGACAGGCGCGCGCGGGGTTTATGCTCATGATTGAAACCGCGTTCAGCGTATTCTGCCGCGAGCAAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCt                                                                                                                                                                        <  1:215093/242‑1 (MQ=255)                                                                          gAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGCACAATCCCTTTTCCTTCGCt                                                                                                                                                             >  2:642340/1‑250 (MQ=255)                                                                           aaCATAAGCTTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCTTAAGGGAACGCGGGGAGAGAGGCTTTCCAGTTGCGACCCAATACGCCATTTATCATAAAAATCATCCCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCAGGATTGAAAACGCTTTCAGCGTTTTCTGCCCCGAGCCAGATTTTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTaa                                                                                                                                                           <  1:677381/251‑1 (MQ=255)                                                                                 aGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTc                                                                                                                                                     >  1:55096/1‑251 (MQ=255)                                                                                  gCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATCACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTcc                                                                                                                                                    >  1:287425/1‑251 (MQ=255)                                                                                    cTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCAt                                                                                                                                                  >  1:462776/1‑251 (MQ=255)                                                                                                   tGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATc                                                                                                                                   >  1:559542/1‑251 (MQ=255)                                                                                                   tGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATc                                                                                                                                   >  1:406900/1‑251 (MQ=255)                                                                                                    gTAACCCCATTTTTCATTGCCCGTAATGGACCGCGGTGAAAGGGGCGTCCCAGTTGCAAACCAATCCCCCATTTATCATAAAAACCATCCCAGCCAGCCAGGCGCGCGCCGGGTTTATGCTCATGATTAAAACCGCTTCCAGCGTATTCTCCCTCGAGCCAGATTTTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAAt                                                                                                                                    <  1:170019/249‑1 (MQ=255)                                                                                                    gTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACACTCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATcc                                                                                                                                  >  1:10399/1‑251 (MQ=255)                                                                                                    gTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATcc                                                                                                                                  >  2:105295/1‑251 (MQ=255)                                                                                                       accccATTTTTCATTCCCCGAAAGGGAACGCGTGGAGAGAGGCTTCCCAGTTCCGACCCAATCCCCCATTTATCATAAAAATCATCCCAGCCAGACAGGCGCGCGCCGGTTTTATGCTCATGATTAAAACCGCTTCCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGc                                                                                                                               <  1:603638/248‑1 (MQ=255)                                                                                                              ttttCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAAccc                                                                                                                       >  1:367506/1‑250 (MQ=255)                                                                                                              ttttCATTGCCCGAAATGGAACGCGGTGAGAGAGGCTTTCCAGTTGCAAACCAATACGCCATTTAACTTTAAAATCATCCCAGCCAGACGGGCGCGCGCCGGTTTTATGCTCATGTTTAAAAACGCGTTCAGCTTATTCTGCCTCGAGCCAGATTTTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAAcc                                                                                                                        <  1:143152/250‑1 (MQ=255)                                                                                                               tttCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACcgc                                                                                                                      >  2:499318/1‑251 (MQ=255)                                                                                                                     gcccctaaagggaccggggTGAGAGGGGCTTCCCAGTGGCAAACAAATACGCCATTTATCATAAAAACAATCCCACCCAGACGGGCGCGCGCGGGGTTTATGCTCATGATTAAAACCGCGTCCAGCGTTTTCTCCCCCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAg                                                                                                                 <  1:304737/233‑1 (MQ=255)                                                                                                                         cGTAATGGAACGCGGTGAGAGAGGCGTTAAAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATc                                                                                                            >  1:488019/1‑251 (MQ=255)                                                                                                                            aaTGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGACAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACACGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTCATTTGTCCATCGACATACCCGAATCCAGCACAAACC‑AGCCAGTTATCCtt                                                                                                         >  2:90576/1‑251 (MQ=255)                                                                                                                                       gTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCCGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGACCAATCCCTTTTCCTTCCCTAACTTGTCCATCGGCATAACCGCATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCggtggtt                                                                                              >  2:223048/1‑250 (MQ=255)                                                                                                                                       gTGAGAGAGGCGTCCCGGTGGCAAACCAATCCCCCAGTTATCATAAAAATCAACCCAGCCAGACGGGCGCGCGCCGGGTTTTGGCTAATGATTAAAAACGCGTACAGCGTATTCTCCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGgtggtg                                                                                              <  1:642340/251‑1 (MQ=255)                                                                                                                                              agGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGATTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGAGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCAGTGGTGAATAAAc                                                                                       >  1:383357/1‑251 (MQ=255)                                                                                                                                                 cGTTCCAGTTGCGAACAAATACGCCAGTTATCATAAAAATCAAACCAGCCAGACAGGCGCGCGCCGGTTTTATGCTCATGATTGAAAACGCGTCCAGGGTATTCTCCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGAt                                                                                    <  1:105295/251‑1 (MQ=255)                                                                                                                                                                 cAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGAc                                                                     >  1:626499/1‑250 (MQ=255)                                                                                                                                                                  aaTACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGGGGTGAATAAACGATGGTGGTTAAGTCGAcat                                                                   >  1:350700/1‑251 (MQ=255)                                                                                                                                                                              ttaataaaaaTCATCCCAGCCAGCCGGGCGCGCGCCGGGTTTATGCTCATAATTAAAACCGCGTTCAGCGTTTTCTGCCCCGAGCCAGATATTACGTGCCCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCa                                                        <  1:570271/244‑1 (MQ=255)                                                                                                                                                                                       aTCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGc                                              >  1:12733/1‑251 (MQ=255)                                                                                                                                                                                         cATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGAGACCGCCACTCAACACCCGCAAGACAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGATGGTGAATAAACGCTGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCt                                            >  1:326342/1‑251 (MQ=255)                                                                                                                                                                                              aagccagACGGGCGCGCGCGGGTTTTAGGCTAATGATTAAAAACGCGTTCAGCGTATTCGGCCTAGATCAAGATATTCGGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGc                                       <  1:223048/250‑1 (MQ=255)                                                                                                                                                                                                  cagACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATc                                    >  1:289627/1‑250 (MQ=255)                                                                                                                                                                                                               gccgggaatATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGTTGAATAAACGAAGGTGGTTAAGTCGACATCATGTTTCCCACTATCCGGACATTCGGCATCAATTCCTGTTGAGc                      >  2:540508/9‑251 (MQ=255)                                                                                                                                                                                                                   ggTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCAAGc                  >  2:383568/1‑251 (MQ=255)                                                                                                                                                                                                                       tATGCTCATGATTGCAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCCTCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATcc                                                  >  2:251803/1‑215 (MQ=255)                                                                                                                                                                                                                        aTGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTACATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATAc             >  1:402067/1‑251 (MQ=255)                                                                                                                                                                                                                              aTGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGct       >  1:200416/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaCGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCa  >  1:96074/1‑247 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         CGCTGGTTACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCA‑AAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCA  >  NC_000913/1090263‑1090739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑