Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,075,234	C→T	V1217I (GTA→ATA)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,075,234	0	C	T	100.0%	223.9 / NA	75	V1217I (GTA→ATA)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (55/20);  total (55/20)

TACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTACCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTT  >  NC_000913/1074990‑1075477                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       taCAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCt                                                                                                                                                                                                                                                  >  1:96786/1‑251 (MQ=255)   cAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCTTCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCATTTGACTACG‑TTCGCTG‑ACTGCCGATGGCAATTCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTCTCTGGCTGc                                                                                                                                                                                                                                                >  1:222278/1‑251 (MQ=255)     gCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGccc                                                                                                                                                                                                                                              <  1:9921/251‑1 (MQ=255)     gCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGccc                                                                                                                                                                                                                                              >  1:22395/1‑251 (MQ=255)        gTTCCAGAAGGATATTGCTTTCGCCACGGGAAAAACCCTGCACCGAAACACTTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCAcc                                                                                                                                                                                                                                           >  1:598859/1‑251 (MQ=255)                    tatTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCCCCGCCAGCAcggcg                                                                                                                                                                                                                               >  1:342924/1‑251 (MQ=255)                              gCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑g                                                                                                                                                                                                                      >  1:111880/1‑249 (MQ=255)                                cACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGAGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGCTGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGTCTGCCCACCGCCAGCACGGCGGCGGGCT‑ggggc                                                                                                                                                                                                                  >  2:417295/1‑247 (MQ=255)                                    ggCAAAACCCTGCACCGAAACAATTGCGCCATCCCGCGCGGCCACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATACGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAgcg                                                                                                                                                                                                              >  1:561149/1‑251 (MQ=255)                                              tGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGACAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCtc                                                                                                                                                                                                    >  2:203962/1‑251 (MQ=255)                                                cACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGAAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATACGGCCACAGTATCTGGCTGCCACCCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCtcat                                                                                                                                                                                                  >  2:615604/1‑251 (MQ=255)                                                 aCCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGATGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTTGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGGCAGCGCATCCTTCtcatc                                                                                                                                                                                                 >  2:4672/1‑251 (MQ=255)                                                         aaTTGTGCCAGCACGCGCGGCAACTGCTTCACACACTGCACGAAGCTGATCCGAATCCCCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCGGGCTGCCCACCGCCAGCACGGCGGAGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCa                                                                                                                                                                                          >  2:415474/1‑250 (MQ=255)                                                          aTTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAAt                                                                                                                                                                                        >  1:346512/1‑251 (MQ=255)                                                            tgtgCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATac                                                                                                                                                                                      >  1:343696/1‑251 (MQ=255)                                                              tgCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATAACCGTGGCAGATCACC‑GCATCACACGGT‑TGA‑GCGGATATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCAATAA‑CTGACGATGCAGCGCGTCATCCGGCCACCGTATCTGGCTGCCCCCCGCCAGCCCGGCGGCGGGCT‑GAGCAAGCGCATCCTGCTCATCATCGGCAATaacc                                                                                                                                                                                    >  2:533646/1‑248 (MQ=255)                                                                cacacccGCTCGGAAACTGCTCCACCAAAGGCGTGAAGCGGATCCGAATCACCTTGGAAGACCACC‑GCATCAACCGTT‑TGA‑GCGTTTTTATTTTCCGCTTGCGCCTGTTAAATTCG‑TCCGCTG‑ACTGCCTAGGGAAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGCATCTGGCTGCCCTCCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACacaa                                                                                                                                                                                  <  1:49622/247‑1 (MQ=255)                                                                      cgcgcgGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACAcgcg                                                                                                                                                                            <  1:106655/251‑1 (MQ=255)                                                                         gcgGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTCTCTTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAC‑CTGACTATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCACACCGCCAGCAAGGCGGCCATCT‑GCGCCAGCGTATCCTGCTCATCATCGGCAATACacaaccagctccc                                                                                                                                                                         >  2:561220/1‑243 (MQ=255)                                                                                    ttCACACAATCCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCCCAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGgcagc                                                                                                                                                              >  2:626166/1‑250 (MQ=255)                                                                                          caATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCTCCAGTTAAATACG‑TTCGCTG‑ACGGCCGATGGCAATGCCTTCACTAA‑CTGACGATTCAGCTCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACTGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGgccacagcatcc                                                                                                                                                       >  1:539678/1‑241 (MQ=255)                                                                                           aaTGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCCTCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTccc                                                                                                                                                      >  2:646636/1‑250 (MQ=255)                                                                                           aaTGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGAATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCCTCATCGGCAATACACCACACGAGC‑CACAGCGGCAACAGCGTcc                                                                                                                                                       >  2:18083/1‑250 (MQ=255)                                                                                            aagcgcGAAGCTGTTCCCAATCCCCGGGGAAGACCCCC‑CCACCAAACGTT‑TGA‑GCTGTTATTTTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCCCTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCGGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAg                                                                                                                                                     <  2:63185/249‑1 (MQ=255)                                                                                                   aaGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCt                                                                                                                                                                                                        <  1:14818/194‑1 (MQ=255)                                                                                                   aaGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCt                                                                                                                                                                                                        >  2:14818/1‑194 (MQ=255)                                                                                                      ggattCCGATTCCCCGTGGA‑GATCACCCGCATCAACCGGT‑TGG‑GCGGTTATATTTTCCGCTTTCCCCAGTGTACTACG‑TT‑GCCGTACTGCCGATGGCAATGCCTTCCCTAA‑CTGGCGAGGCACCCCGCCACCCGGCCCCAGTATCTGGCTGCCCCCCGCCAGCACGGCGGCGAGCT‑GAGGCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGtt                                                                                                                                          <  2:90578/247‑1 (MQ=255)                                                                                                      cTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGtt                                                                                                                                          >  1:376827/1‑251 (MQ=255)                                                                                                      cTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGtt                                                                                                                                          >  1:37242/1‑251 (MQ=255)                                                                                                       tGATCAGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGAGACATCCGGCCACAGTATCTGGCTTCCCACCGCCAGCACGGCCGCGCGCT‑GAGACAACGCATCCTGCTCATCATCGGCAACACACAACCCACGCTCAG‑GCGCAAGCCGCGACCCGGTGG‑TGCGTTc                                                                                                                                         >  2:516096/1‑251 (MQ=255)                                                                                                       ggatcacatgcACCGTGAAAGATCTCC‑GCAT‑ACACAGTGTGA‑GGGGTTGTATTTCCCGTGTTCCCCAGTTAATTCCG‑TTCTCTG‑CCTACGGATGGAAAGGCCTTCATTAA‑CTGACGATGCAGCGCTTAACCCGGTCCCAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCAGCAGCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCAAGGTGT‑TGCGtgc                                                                                                                                         <  2:415449/241‑3 (MQ=255)                                                                                                         aTCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GCGTCAGCGCATCCTGCTCATCATCGGCAATACACCACACGCGCCCCC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCAc                                                                                                                                       >  2:136834/1‑251 (MQ=255)                                                                                                               tttccgactggaaaagtcccagcacAAACGGT‑TGATGCGGTTATTTGTGCCCCTTCCGCCGGTTGAA‑ACGGTTCGCGG‑CTTTCCGAGGGAAATGCCTTC‑CTAAACTGACGATGCAGCGCTTCACCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGTAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCTTTCAcc                                                                                                                                      <  2:89542/224‑1 (MQ=255)                                                                                                                 ccccGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TCCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCTTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCgg                                                                                                                                <  1:414575/248‑1 (MQ=255)                                                                                                                 cACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGACCAGGTGT‑TGCGTTCACCCGTCGGc                                                                                                                               >  1:125684/1‑251 (MQ=255)                                                                                                                   ccGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGccc                                                                                                                             >  1:193607/1‑251 (MQ=255)                                                                                                                       ggAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCAcc                                                                                                                                      >  1:89542/1‑238 (MQ=255)                                                                                                                              cACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATc                                                                                                                  >  1:116806/1‑251 (MQ=255)                                                                                                                                     aTCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTtgtg                                                                                                            >  1:210098/1‑251 (MQ=255)                                                                                                                                         aaCGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTcc                                                                                                        >  1:425351/1‑251 (MQ=255)                                                                                                                                           cGGT‑TGA‑GCGGATATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGCCCAGGTGT‑TGCGTTAACCCGTCCGCCCCGGCAGCAATCGTTGTGTTcctc                                                                                                      >  2:138375/1‑250 (MQ=255)                                                                                                                                                tGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATTGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑GGCGTTCACCCGTAGTCCCCGACAGCAATCGTTGTGTTcctgcctg                                                                                                  >  2:169286/1‑251 (MQ=255)                                                                                                                                                  a‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑CTCCCTG‑ACTGCCCATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCTTCATCCGGCCACAGGATCTGGCTGCCCACCGCCAGCCCGGCGGCTAGCTCGA‑TCAGCGCCTCCTGCTCATCATCGGCCATACACAACACCCGCTCAC‑GCGGCATCAGCCGCCAGG‑GTGTACGTTAACCCGTCGGCCCCGGCAGCCATCGGTTTGTTcctgcctacc                                                                                                >  2:164210/1‑248 (MQ=255)                                                                                                                                                   ggCGGTTATATTTCCCCATTTCGCCAGTTAAATACG‑TGCGCTG‑ACTGCCGATGGCAATGCCTTCACAAA‑CTGCCGATGCAGCGCGTCATCCGGCCACAGTATCGGGCTTCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTgcg                                                                                                <  1:533646/250‑1 (MQ=255)                                                                                                                                                      ggTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGcc                                                                                              >  1:207788/1‑250 (MQ=255)                                                                                                                                                          atatTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTc                                                                                         >  1:544255/1‑251 (MQ=255)                                                                                                                                                                  cGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGCCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCACGCCAAAtt                                                                                 >  2:622894/1‑251 (MQ=255)                                                                                                                                                                       cccccAGTTGATTACG‑TGCGCTT‑CCTGCCGAGGGCAATGCCTTCACTAA‑CTGACGACGCAGCGCGTCAGCCGGCCACTGTATCTGGCTGCCCCCCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTa                                                                            <  2:96786/248‑1 (MQ=255)                                                                                                                                                                         gCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTAc                                                                           >  1:357373/1‑250 (MQ=255)                                                                                                                                                                             gTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCGGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAAc                                                                      <  1:82742/251‑1 (MQ=255)                                                                                                                                                                              ttGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTACTGCCTGCGCCAGCTCGCCATATTGCGTACACAACg                                                                     >  2:543288/1‑251 (MQ=255)                                                                                                                                                                              ttGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGAAGCGTCCAGGTGT‑TGCGTTCACCCGTAGGCCCCGGCAGCAATCGGTGTGTCCCTGCCTGCACCAGCTCGCCATACTGCGAACATAACg                                                                     >  2:454442/1‑251 (MQ=255)                                                                                                                                                                                 aaTACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCCGCTCGCCATATTGCGTACATAACGCCt                                                                  >  1:321203/1‑251 (MQ=255)                                                                                                                                                                                  aTACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTg                                                                 >  1:673226/1‑251 (MQ=255)                                                                                                                                                                                  aTACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTg                                                                 >  1:497890/1‑251 (MQ=255)                                                                                                                                                                                     cG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCAATAA‑CTGACGATGCAGCGCGTAATCCAGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAAGTGT‑TGAGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGGCCGCTCGCCATATTGCGTACATAACGCCTGCa                                                               >  1:243938/1‑250 (MQ=255)                                                                                                                                                                                       gtccGCTG‑ACGGCCGATGGCAATCCCTCCACTAA‑CCGACGATGAAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTTCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAAt                                                             <  1:187941/248‑1 (MQ=255)                                                                                                                                                                                          cGCTG‑ACTGCCGATGGCAATGCCTTCACGAA‑CTGACGATGCAGCGCGGCAGCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCt                                                          <  1:415474/251‑1 (MQ=255)                                                                                                                                                                                           gCTG‑ACTGCCGATGGCAATGCCTTCCCTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGACGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCGCGCCAGATTGCGTATATAACGCCTGCAATCCTg                                                         >  2:467570/1‑251 (MQ=255)                                                                                                                                                                                             tG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTgg                                                        >  1:333086/1‑250 (MQ=255)                                                                                                                                                                                                          gCAATGCCGTCACTAA‑CTGCCGATGCAGCGCGTCATCCGGCCACAGTCTCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GCGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGCGT‑TGCGTTCACCCGTCGGCCCCGGCCGCAATTGTTGTGTTCCTGCCTCCGCCCGCTCGCCACTTTGCGTTCATAACGCCTGCAATGCTTGACGATTagctccc                                           >  2:537859/1‑248 (MQ=255)                                                                                                                                                                                                            aaTGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCa                                         <  1:308300/251‑1 (MQ=255)                                                                                                                                                                                                             aTGCCTTCCCTAA‑CGGCCGATGCAGCGCTTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCAt                                        <  1:467570/251‑1 (MQ=255)                                                                                                                                                                                                              tGCCTTCACTAA‑CTGACGATGCAGCGCTTCATCCGGCCACAGTATCTGGCTGCCCCCCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCAt                                        <  1:615604/250‑1 (MQ=255)                                                                                                                                                                                                                cctccACTAA‑CTGCCGAGGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTcc                                     <  1:516096/247‑1 (MQ=255)                                                                                                                                                                                                                   tCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGc                                  >  1:545165/1‑251 (MQ=255)                                                                                                                                                                                                                     aCTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGc                                  >  1:664067/1‑249 (MQ=255)                                                                                                                                                                                                                      cTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCATACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGt                               >  1:573167/1‑251 (MQ=255)                                                                                                                                                                                                                               cGATGCAGCGCGTCACCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAg                       <  1:646636/251‑1 (MQ=255)                                                                                                                                                                                                                                gATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGc                      >  1:630653/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAggc             >  1:294460/1‑248 (MQ=255)                                                                                                                                                                                                                                           tCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCATGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa            >  1:624397/1‑250 (MQ=255)                                                                                                                                                                                                                                               ccGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCg       >  1:526822/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ggCCACAGTTTCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGc     <  1:599133/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cacaGTATCTAGCTGCCAACCGCCAGCAAGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑TCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGAGCCATCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCCGTTCATTTAGCGGATGCTTCAATGCGGCttt  >  2:585539/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       TACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACC‑GCATCAAACGGT‑TGA‑GCGGTTATATTTTCCGCTTTCGCCAGTTGAATACG‑TTCGCTG‑ACTGCCGATGGCAATGCCTTCACTAA‑CTGACGATGCAGCGCGTCATCCGGCCACAGTACCTGGCTGCCCACCGCCAGCACGGCGGCGAGCT‑GAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAC‑GCGGCAGCAGCGTCCAGGTGT‑TGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTT  >  NC_000913/1074990‑1075477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑