Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	97.8%	143.5 / NA	46	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/23);  minor base G (0/1);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACG  >  NC_000913/822281‑822764                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              cGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTAcc                                                                                                                                                                                                                                           <  1:258443/251‑1 (MQ=255)   ggCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCg                                                                                                                                                                                                                                         >  1:659360/1‑251 (MQ=255)              cATAGCAGCCGTAAAGCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGTTTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGttt                                                                                                                                                                                                                              <  2:582948/251‑1 (MQ=255)                    agcCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGCTCCTGCGTTTCCAttt                                                                                                                                                                                                                        >  1:267848/1‑250 (MQ=255)                     gcCGTAAATCAGATAGCTGACGACCTCCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGt                                                                                                                                                                                                                       <  1:133730/251‑1 (MQ=255)                      cctgaagtCAGATAGCCGACGTCCCCCAGCCCGACCGCTCTAAGCGTCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTGTTTGGCGTAGCCCACCAGCACCACAATCACCGCGATAAAAAACAGCCATGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTGTTACCCGATCCTGCGTTTCCATTGTc                                                                                                                                                                                                                      <  2:424548/244‑1 (MQ=255)                                    gCTGAAGACCACCAGCCCGACCGCACTAAGCCGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAAACCACCAGCACCACAATCACCCCGATAAAAAACATCCAGGAGAGGATCTTCTTTGCTAAGCCCCAGCGCGGGTGTGCTTTACTCATCAGGGTTTTACCCCCGTGCTTTCAGTTTTTACCCGAACCTGCGTTTCCACTGGCGGTGGTgcgggcgg                                                                                                                                                                                                        >  2:140085/1‑251 (MQ=255)                                     cTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTgcgggcgg                                                                                                                                                                                                        >  1:601195/1‑250 (MQ=255)                                      tGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGAt                                                                                                                                                                                                      >  1:400697/1‑251 (MQ=255)                                                              tAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCgtgg                                                                                                                                                                              >  1:409524/1‑250 (MQ=255)                                                                    gcgcAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCg                                                                                                                                                                        <  1:176603/251‑1 (MQ=255)                                                                             gATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCACGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACAGGGCGTGTGTGCAGGAtgccagc                                                                                                                                                               >  1:353086/1‑251 (MQ=255)                                                                                        cgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGcc                                                                                                                                                    >  1:51217/1‑251 (MQ=255)                                                                                              gacTTCCCGACCTCTTTCCAGGCCCTTTTTTTGGCCGAGGCCCCCAGCAACACAAACCCCGCGATAAAAAACAGGCAGGTTAGGATCCTCCTTGCTAAGGTCCTGGGCGGGTGGGATTTACTCATCAGGGGTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTGCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTTCCGGAAGCCAGCCAACCAGCGCCGGGaa                                                                                                                                               <  1:595614/248‑1 (MQ=255)                                                                                               ccTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGGGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAgg                                                                                                                                             >  1:276980/1‑250 (MQ=255)                                                                                                    cagcccTCTTCCCAGGCCACTTTTTTGGCGTAGACCACCAGCAACACAATAACCACGCTAAAAAACATCCAGGTGAGGATCTTCTTTGATAAGCGCCAGCGCGGGTGTGATTTCCTCATCAGGGTTTTACCCCCGAGTGTTCAGTTTTTACCGGATCCTGCGTTTCCATTGCCGGTTTTGCAGGCGGTTCAAACCGAGCCCGACGTGGCTGGCGTGCCTGTAGGCAGCAAACCAGCGCCGGGaaagggcgg                                                                                                                                        >  2:134005/5‑244 (MQ=255)                                                                                                             tCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGt                                                                                                                                 <  1:290487/249‑1 (MQ=255)                                                                                                               ccAGTCCACTTTTTTTGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGaa                                                                                                                             <  1:68777/251‑1 (MQ=255)                                                                                                                          ttttGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGAGGAACGCCAGCACGc                                                                                                                  >  1:145911/1‑251 (MQ=255)                                                                                                                                   agcccaccaGCAACCAAAACACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTGTACCCGATCCGGCGTTTCCATTGTCGGTGGTGCGGGCGGATCAACCTGCGCCATACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCATGCTTTTGGTCa                                                                                                         <  2:578455/248‑1 (MQ=255)                                                                                                                                   aGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCa                                                                                                         <  2:663763/251‑1 (MQ=255)                                                                                                                                   aGACCACCAGCAACACAATCACCGAGATAAAAAACAGCCCGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGCTGTGCGGGCGGCTCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGGCCGGCAACCAGCGCCCGGAAGTGGCGGAAAAAGGGGGACGCCCGCACGCCTTGGGTc                                                                                                          >  2:400052/1‑250 (MQ=255)                                                                                                                                    gACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGGAAAAAGTGGAACGCCAGCAAGCTTTTGGTc                                                                                                          >  1:364519/1‑249 (MQ=255)                                                                                                                                               acacAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGGAAAAAGGGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAgg                                                                                             <  1:265354/251‑1 (MQ=255)                                                                                                                                                    aTCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCCGAGGCCAGCCCACCAGCGCAGGGAAGTAGCGTAAAAAGGGGGAAGCCAGGCCGCTTTTTGTCAGATGccacccggggcgg                                                                                        >  2:530755/1‑243 (MQ=255)                                                                                                                                                        ccGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAA‑GTGGAACGCCAGCACGCTTTTGGTCAGGTTCCCCCAGGTGCGTTTggg                                                                                   <  2:648354/251‑1 (MQ=255)                                                                                                                                                         cgcgATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGAGTGTGCCGGGAGCCCGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGTACGCCAGCACCCCTTTGGCCCGGTTCCCACAGGGGCGGTTggg                                                                                   >  2:131009/1‑251 (MQ=255)                                                                                                                                                                     cAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGTGGGTGGGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCTCCAGGTGCGTTTGGGCAGCATGGTTTc                                                                       <  2:267848/251‑1 (MQ=255)                                                                                                                                                                     cAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTc                                                                       <  1:48288/251‑1 (MQ=255)                                                                                                                                                                      aGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCa                                                                      <  1:656557/251‑1 (MQ=255)                                                                                                                                                                        ccatgTGAGGATCTGCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTGCCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCATCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTGTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATc                                                                    <  1:400052/247‑1 (MQ=255)                                                                                                                                                                          aGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTc                                                                                                          <  1:169887/211‑1 (MQ=255)                                                                                                                                                                            gTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGGGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGAGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAAGAGCGTTTGGGCAGCATGGTTTCATCCAcc                                                                >  1:575721/1‑251 (MQ=255)                                                                                                                                                                                gtaTCTTCTTTGCGAAGCGCCAGCGCGGGTGGGATTGACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCAATGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACctgct                                                            <  2:202315/249‑1 (MQ=255)                                                                                                                                                                                  aTCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGAGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGACGGGAAGTGGCGTAAAAAGTGGAACGCACGCACGCATTTGTGCAGGATCCACCAGGAGCGTTTGGGCAGCATGGGTTCATCAAActgctg                                                           >  1:579603/1‑250 (MQ=255)                                                                                                                                                                                     ttcttTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGGGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACa                                                        >  1:219359/1‑250 (MQ=255)                                                                                                                                                                                             tAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGc                                               >  1:447060/1‑251 (MQ=255)                                                                                                                                                                                                  gcCAGCTCGGGTGTGATTTACTCCTCAGGGTTTTACCCCCGTGGTTTCAGTTTTTCCCCGAGCCTGCGTGTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTGTGGTCAGGTTCCACCAGGTGCGTTTGGGCATCATGGTTTCATCCACCTGCTGACAATCTGCGGCaataa                                          <  2:644313/251‑1 (MQ=255)                                                                                                                                                                                                    cAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATg                                        <  1:609200/251‑1 (MQ=255)                                                                                                                                                                                                              gtgATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGAt                              <  1:225907/251‑1 (MQ=255)                                                                                                                                                                                                                  tttactattcaGGGTTTTACCCCCGTGTTTTCAGTATTGACCCGGTCCTGCGTTTCCATTGGCGGTTGTGCGGGCTGATTATCCTGCGCCAGACGTGGAGTGTGTGCCGTAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCAGGGTTTCATCCACCTGCTGACAATTTGCGGCAATAATGCCGTTCAGATTAt                           <  2:409524/242‑1 (MQ=255)                                                                                                                                                                                                                         atcaGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGc                   >  1:680394/1‑251 (MQ=255)                                                                                                                                                                                                                                 tttACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGt            <  1:477457/250‑1 (MQ=255)                                                                                                                                                                                                                                        ccGTGTTTTCAGTCTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGAACAACCTGCGCCAGACGGGGCGAGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTCGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGGTAAAATg     >  1:421352/1‑250 (MQ=255)                                                                                                                                                                                                                                          gtgtTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACg  <  1:325513/251‑1 (MQ=255)                                                                                                                                                                                                                                           tgtTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAAAGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACg  >  1:46875/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              CGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACG  >  NC_000913/822281‑822764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑