Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,508 A→G Q724Q (CAA→CAG)  thrA → fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,5080AG95.0% 170.9 / ‑2.7 60Q724Q (CAA→CAG) thrAfused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (24/33);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

ATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑ACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCT  >  NC_000913/2261‑2745
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             
atatCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTc                                                                                                                                                                                                                                            <  1:264255/251‑1 (MQ=255)
  agcTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTcg                                                                                                                                                                                                                                           <  1:29525/248‑1 (MQ=255)
         gCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGACATTGAACCTGTG‑CTGCCCGCAGCGTTTAACGCTGTGGGGGATGTTGCCGCTTTTATGGCGAATCTGTCACC‑GCTcgacataccc                                                                                                                                                                                                                                   >  2:620782/1‑245 (MQ=255)
          cAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATctct                                                                                                                                                                                                                                  >  1:278477/1‑251 (MQ=255)
               tAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGcc                                                                                                                                                                                                                             >  1:185643/1‑251 (MQ=255)
                    gAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGAAATTGAAATTGAACCTGTG‑CTGCCCGCAGGGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCCCC‑GCCCCGCGATCTCTTTGCcgcgcg                                                                                                                                                                                                                        >  2:154786/1‑251 (MQ=255)
                                       aGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGtgat                                                                                                                                                                                                     <  2:573551/251‑1 (MQ=255)
                                         gCGACTACGCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGtgatga                                                                                                                                                                                                   <  2:327009/251‑1 (MQ=255)
                                           gacgcatttGGCGCGGGAAATGGGTTATCCCGAATCGGATCCGCGAGATGAGTTTTCTGGTATGGATGTAGCGGGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGAGCTCTTTGCCGCGCGCGTGTCGATGGCCCGTGATGAAg                                                                                                                                                                                                 <  2:448640/243‑1 (MQ=255)
                                                            aaaTGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTAt                                                                                                                                                                                <  1:479909/251‑1 (MQ=255)
                                                                gggTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGtt                                                                                                                                                                             <  1:612517/250‑1 (MQ=255)
                                                                gggTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTg                                                                                                                                                                            <  1:154786/251‑1 (MQ=255)
                                                                 ggTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGGGGGTGATGTTGCCGCTTTTATGGCGACTCTGTCACA‑GCCCGCCCGTCTCTTTGCCGCGCGCATGGCGAAGGCCCGTGATTACGGGCAAGTTTTTCGCTATTTTgg                                                                                                                                                                           >  2:307617/1‑251 (MQ=255)
                                                                 ggTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGGGCTGGCGGGTATTGAAATTTAACCTGTG‑CTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGCATTTGTCACA‑ACTCGACCATCTCTTTTCCCCGCGCGTGGCGAAGGGCCGTGCTGTAGGGAAAGtttttgatcgggaggg                                                                                                                                                                           >  2:154814/1‑239 (MQ=255)
                                                                 ggTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑ACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGtttt                                                                                                                                                                                       <  1:154814/239‑1 (MQ=255)
                                                                        ccGAACCGGATCCGGTAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCGATTGATTCCCGCTCGGGAAACGGGACGTGAACGGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATAtt                                                                                                                                                                    <  2:247719/251‑1 (MQ=255)
                                                                         cGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTTGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGGTATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGAC‑CGACGATCTATTTGCCGCGCGCGTGGCAAAGGCCCGGGATGTAGGATAAGGTTTTCGCTATGTTGGCGATAttt                                                                                                                                                                   >  2:100415/1‑250 (MQ=255)
                                                                           aaCCGGATCCGCGAGATGATCTTTCTGGTATGGCTGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGCAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTCTGGCGAATCTGTCACA‑GCTCGACGGTCTCTTTGCCGCGCGCGTGGGGAAGGCCCGTGGTGGAGGAAAAGTTTTGCGCTATGTTTGCAATATtgat                                                                                                                                                                 >  2:293131/1‑251 (MQ=255)
                                                                              cGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGCAGGAAAAGTTTTGCGCTATGGTGGCAATATtgttga                                                                                                                                                               >  1:45148/1‑250 (MQ=255)
                                                                              cGGAGCCGCGAGATGATCTGTCTGGGATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGaa                                                                                                                                                              <  2:185643/251‑1 (MQ=255)
                                                                                gatccGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGa                                                                                                                                                            <  1:219246/248‑1 (MQ=255)
                                                                                    cgcgAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGGTGGAGGaaaa                                                                                                                                                                                            >  2:32672/1‑215 (MQ=255)
                                                                                    cgcgAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGaaaa                                                                                                                                                                                            <  1:32672/215‑1 (MQ=255)
                                                                                       gagaTGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATggtgcc                                                                                                                                                     >  1:644540/1‑247 (MQ=255)
                                                                                           tgatCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGtgcctgcc                                                                                                                                                 >  1:320017/1‑251 (MQ=255)
                                                                                                 ttCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTg                                                                                                                                            <  1:596173/250‑1 (MQ=255)
                                                                                                     ggTATGTATGTAGCGCGTAAGCGATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTGTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGaa                                                                                                                                          <  1:285576/248‑1 (MQ=255)
                                                                                                        atggatggAGCGCGTAAGTTATTGATTTCCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGATTTTAACGCTGAGGGTGATGTTGCCGCTTTTAGGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTg                                                                                                                                     <  2:644540/250‑1 (MQ=255)
                                                                                                         tggttgtagcgcGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTAGGGCGAATCTGTCACA‑GCTCGACGATCCCTTTGCCGCGCGCGTGGCGAAGGGCCGTGCTGAAGGAAAAGTTTTGCGCTATGTTGGCAATTTTGATGAAGAAGGGGCCTGCCGCGTggaacttgcc                                                                                                                                   >  1:517042/9‑242 (MQ=255)
                                                                                                                   gcgTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATg                                                                                                                         <  1:37647/251‑1 (MQ=255)
                                                                                                                               gATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCg                                                                                                              <  1:620782/250‑1 (MQ=255)
                                                                                                                               gATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCg                                                                                                              <  1:237226/250‑1 (MQ=255)
                                                                                                                                agtcgcgctcgTGAAACGGGACGTGAACGGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCt                                                                                                            <  2:320017/246‑1 (MQ=255)
                                                                                                                                   ctcgctcgTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGtt                                                                                                         >  1:8201/1‑251 (MQ=255)
                                                                                                                                     cgctcgTGAAACGGGGCGTGAACTGGAGCTGGCGGATATTGAAATTTAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGtt                                                                                                         <  1:561373/249‑1 (MQ=255)
                                                                                                                                     cgctcgTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGtt                                                                                                         <  1:55438/249‑1 (MQ=255)
                                                                                                                                                     cGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAAAGATACGCTGTTCAAAGTGAAAAATgg                                                                                          >  2:385953/1‑248 (MQ=255)
                                                                                                                                                             ggAGCTGGTGGATATGGAAATTGTTCC‑GTGGCTGCCCGCAGTGTTTAACGCTGAGGGGTATGTTGCCGCTTTTATGGCGAATCTGGCACA‑GCTCGACGATCTCTGTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGGTTTGCGGTATGTTGGCAATATTGAGGAAGACGGTGCCTGCCGCGTGAAGATTGCTGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGcc                                                                                <  2:230280/250‑1 (MQ=255)
                                                                                                                                                               ggCTGGCGGATATTGAAATTGAACCTTGG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTg                                                                             <  2:177021/250‑1 (MQ=255)
                                                                                                                                                                 gtGGCGGATATTGAAATTGAATCTTGG‑CTGCCCGCAGAGTTTAACGCTGAGGGGGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGATATGTTGGCAATATTGATGAAGATGGTGCCTTCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGc                                                                           <  2:373143/250‑1 (MQ=255)
                                                                                                                                                                          attgaaattgaaCCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGATCTGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGGGGATGGTAATGATACGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTtatat                                                                  >  1:379434/1‑250 (MQ=255)
                                                                                                                                                                                  tgaaCCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACtatt                                                          >  1:423890/1‑251 (MQ=255)
                                                                                                                                                                                  tgaaCCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGGGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACtat                                                           <  2:549721/250‑1 (MQ=255)
                                                                                                                                                                                   gaaCCTGTG‑CTGCCCGCAGAGTTTAACGCTGAGGGGGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTTCCGCGCGCGTGGCGAAGGCCCGTGATGGAGGGAAAGTTTTGCGCTATGTTGGCAATATTGATGACGATGGTGCCTGCCGCGTGATGATTGCCGAAGTGGATGGTAATGAACCGCTGTTCAAAGTGAAACATGTCGAAAACGCCCTGGCCTTTTATAGCCACtatt                                                          >  1:584697/1‑250 (MQ=255)
                                                                                                                                                                                     aCCTGTG‑CTGCCCGCAGAGTGTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATc                                                       <  1:307617/251‑1 (MQ=255)
                                                                                                                                                                                         gtg‑CTGCCCGAAGAGTTTAACGCTGAGGGTGATGTTGCCGCGTTTATGGCGAATCTGTCACA‑GCGCGACGTTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGcc                                                   <  2:224017/251‑1 (MQ=255)
                                                                                                                                                                                                    cAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGAt                                                                                                                 <  1:614356/179‑1 (MQ=255)
                                                                                                                                                                                                           taacgttgagggtattggtGCCTCTTTGATGGTGAATCTGTCCCA‑GCTCGATGACCTCTTGGCCCGGCGAGGGGCGAAGGCTCATTATGAAGGAAAAGTTTTTAGCTATGTTGGCAAGATTGCTGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAAGGATCCGCTGTTCAAAGTGAACAAGGGCGTAAACGCCCTGGCATTTTATAGATACTATTATGAGGCGATGCCGTTGGTGCTg                                   <  2:89258/232‑1 (MQ=255)
                                                                                                                                                                                                            aaCGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGtgctgc                                  <  2:611759/250‑1 (MQ=255)
                                                                                                                                                                                                             aCGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGCAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGCAAACGCCCTGGCCTTTTATACCCACTATTATCAGCCGCTGCCGTAGGTGCTgcgc                                >  1:652199/1‑251 (MQ=255)
                                                                                                                                                                                                                 tgAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATATCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCggt                             >  1:34952/2‑249 (MQ=255)
                                                                                                                                                                                                                     ggTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATAGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATgg                        >  1:73643/1‑251 (MQ=255)
                                                                                                                                                                                                                       tGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTg                      >  1:391545/1‑251 (MQ=255)
                                                                                                                                                                                                                        gttggtGCCTCTTTTATGTCGAAGCTGTCACA‑GCTAGACGATCTCTTGGCCGCGCGCGTGGCGAAGGCCCGTGATGAATGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTATAGAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGc                     <  2:423890/246‑1 (MQ=255)
                                                                                                                                                                                                                         gttttGCCGCGTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCg                    <  2:186987/248‑1 (MQ=255)
                                                                                                                                                                                                                            ttGCCGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGCAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGTTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGGGAACAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCCGCCACTGCCGTTGGTGCTGCGCGGATATGGTGCGGGc                 >  1:451180/1‑251 (MQ=255)
                                                                                                                                                                                                                                cGCTTTTATGGCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATg             <  1:385953/251‑1 (MQ=255)
                                                                                                                                                                                                                                         ggCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGCTGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGCTCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTCTAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTAc      >  2:225365/1‑249 (MQ=255)
                                                                                                                                                                                                                                          gCGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGc   >  1:309328/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cGAATCTGTCACA‑GCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCt  >  1:502664/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             
ATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTG‑CTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACA‑ACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCT  >  NC_000913/2261‑2745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑