Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,624	C→T	noncoding (71/76 nt)	lysT →	tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,624	0	C	T	96.1%	146.3 / ‑4.3	52	noncoding (71/76 nt)	lysT	tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (26/23);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

AACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAG  >  NC_000913/780378‑780866                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     aaCGCGATGTAATGCACAAAACAAGAAAAGAAGTCGCATGATTTCTGGGCGTGTCGTGCGACACATAAGCAGTTGAGTG‑ATCTACATAGAAATTT‑TTGTTGCGCTAAAGTCTGAAATCCGAAATATATTCCGCCGTTGCCACGGGATATCAAACAAAACGTAAGCAATGAAACAGAGGGTCGTTAGGTCGGTTGGAAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAcccga                                                                                                                                                                                                                                                   >  2:379646/1‑248 (MQ=25)       aTGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGc                                                                                                                                                                                                                                            >  1:43157/1‑251 (MQ=255)           aaTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGCGCAGTTGACTTTTAATCAATTGGTCGCGGGTTCGAATCCTGCACGACTCACCAATCGCTa                                                                                                                                                                                                                                          >  2:168994/1‑249 (MQ=255)           aaTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTaa                                                                                                                                                                                                                                         <  1:541168/250‑1 (MQ=255)             tGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccagctaggaagagcgg                                                                                                                                                                                                                                      >  2:14342/1‑237 (MQ=255)               cATAACACACGACCAGCCGTCGCATTCTTTATGGTCGTGTCGAGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGCTATCAACCAAACCGAAAGCAACGAAACAGAGGGTCGTTAGCTCAGTTGGTAGAGCATTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACAAATCGTTAAggagg                                                                                                                                                                                                                                    >  2:308950/1‑248 (MQ=37)               cATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTCATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑gg                                                                                                                                                                                                                                   >  1:494924/1‑251 (MQ=255)                    cacacGAACAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAATCACCAATCGCTAAGGT‑GGAAg                                                                                                                                                                                                                                >  2:491684/1‑249 (MQ=255)                              aaaGTGGCATTATTTCTGGTCGTGGCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATAGAAATTTGTTGT‑GCGCTCAAGTTTGAAATCAGTAATATATGCCGCCTTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAGCAATTGGTCGCAGGTACGAATCCTGCACGGCTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAAc                                                                                                                                                                                                                    <  2:280396/250‑1 (MQ=38)                                 gcagCATTAGTTCTGGCTGTGTCGTGCGAATCATTAGTATTTGAGTG‑ATCTACATAGAAATTT‑TTGTTGCGATCAAGTCTGAAATCAGTAATATATGCCGTCGTTGCTACGGGATATCATACAAACCGAAAGCAACGAAAAATTTGGTCGTTAGCCTAGTTGGTAGAGCAGTTGACGTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGCCTCTCCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTg                                                                                                                                                                                                                 <  2:173868/248‑1 (MQ=255)                                     cATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGCAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCAAAGGC‑GGAAGCGGTAGTAAAACGTGAAgg                                                                                                                                                                                                             >  1:287919/1‑251 (MQ=255)                                     cATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAgg                                                                                                                                                                                                             <  1:283770/251‑1 (MQ=255)                                                       cgtgCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCa                                                                                                                                                                                            >  1:344551/1‑250 (MQ=255)                                                          gCGAAGCATAAGCAGTGGA‑TGTATATACATCGAAATTT‑TTCTTGCGCTCAAGTCTGAAATCTGAAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAACCGCTAAGGG‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACgg                                                                                                                                                                                        <  2:207121/251‑1 (MQ=37)                                                                 aTAAGCACTTAAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTGC‑AACGTAGGAAACAGTAGTAAAGCGTGGAGGATAACGTTGCATCAGCAACGGCACGa                                                                                                                                                                                   >  2:89462/1‑249 (MQ=25)                                                                   aaGCAGTTGAGTG‑CTCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTCGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGACTCACCAGTTGCTACAGT‑GGTAGCGGTTGTCAAACGTGAAGGATAACGTTGCGTGAGCAACGTCCCGACggg                                                                                                                                                                               >  2:205297/1‑251 (MQ=37)                                                                           gAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACg                                                                                                                                                                        <  1:342100/250‑1 (MQ=255)                                                                           gAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGa                                                                                                                                                                       <  1:123032/251‑1 (MQ=255)                                                                              tG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGt                                                                                                                                                                    >  1:411208/1‑251 (MQ=255)                                                                               g‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGTGCGAGACGAAGt                                                                                                                                                                    <  1:491684/250‑1 (MQ=255)                                                                                   cTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCgtg                                                                                                                                                                >  2:139328/1‑249 (MQ=255)                                                                                       aTCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAt                                                                                                                                                            >  1:187717/1‑251 (MQ=255)                                                                                                  gtgttgCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGa                                                                                                                                                   <  1:621706/249‑1 (MQ=255)                                                                                                             aaGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAAc                                                                                                                                      >  1:230621/1‑251 (MQ=255)                                                                                                               gTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACAt                                                                                                                                    <  1:535629/251‑1 (MQ=255)                                                                                                                   gaaaacattAATATAGGCCGCCGTTTCCACGTGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACAtag                                                                                                                                  <  2:575240/241‑1 (MQ=255)                                                                                                                          gTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTGCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTgta                                                                                                                         <  1:454406/251‑1 (MQ=255)                                                                                                                                     cgccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCgt                                                                                                              <  1:168994/251‑1 (MQ=255)                                                                                                                                        cgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCgtagt                                                                                                           <  1:139328/251‑1 (MQ=255)                                                                                                                                        cgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCgtagt                                                                                                           >  1:338646/1‑251 (MQ=255)                                                                                                                                         gTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCgtagta                                                                                                          <  1:187633/251‑1 (MQ=255)                                                                                                                                                 gggATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTAAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTAGAATCCGGCACGACTCACCAATCGGTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATCCCGTTGCATGAAAAACGGCCCGAAGGACGGGACGAAGTCGAGTCATCCTGCACGACC‑ACACACTAAACTAGTTAGTGGTAGAAACAAGCGTAGAATCGAGTg                                                                                                   >  2:540487/1‑250 (MQ=21)                                                                                                                                                   gATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGAtt                                                                                                <  1:643672/251‑1 (MQ=255)                                                                                                                                                     tatCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCATTCGATAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGGCGAGTCATCCTGCCCGCCCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGAtt                                                                                                >  1:276847/1‑249 (MQ=255)                                                                                                                                                               acCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTggg                                                                                    >  1:537510/1‑251 (MQ=255)                                                                                                                                                                  gAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTggg                                                                                    >  1:651006/1‑248 (MQ=255)                                                                                                                                                                           gAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTc                                                                                           >  1:4000/1‑232 (MQ=255)                                                                                                                                                                           gAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGAAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTc                                                                                           <  2:4000/232‑1 (MQ=255)                                                                                                                                                                           aaaaaaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCTGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTccc                                                                        <  2:407007/250‑1 (MQ=255)                                                                                                                                                                                            gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCTAAGGGCGAGACGAAGTCGAGTCAGCCTGCACGACCCAC‑CACTAACAtagtt                                                                                                                                <  1:599259/178‑1 (MQ=255)                                                                                                                                                                                                     gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCg                                               >  1:88292/1‑250 (MQ=255)                                                                                                                                                                                                           cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGACCACCTCCCTGACAAGGAGGGGGTCGGCGGTTCGATcacgg                                        >  1:367716/1‑247 (MQ=255)                                                                                                                                                                                                                     ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGGCGAGTCATCCCGCACGACCCAC‑CACTTACATAGTGAGTTGGAGTATCCAGCGTAGTATCGGGATATGAGGTACGCTGGGAGAGCACCTAGCTGAGAAGGAGGGGGTcggcgggtcaataacagcaaaaaaaac                              >  2:356443/1‑230 (MQ=21)                                                                                                                                                                                                                        aTCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCcaccac                           >  1:187864/1‑251 (MQ=255)                                                                                                                                                                                                                         tCAATTGGCCGAAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGCAGGATAACGTTGCATGAGCAACGGCCCGCAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTCTCCAGCGTAGTATCGGGTGATTATCTCAGCTGGGAGAGCACCTCCATTACAAGGAGGGGGTctgcgggtgaggtcgggagaggcacgtgg                            >  2:69781/1‑226 (MQ=37)                                                                                                                                                                                                                         tCAAGTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGGAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTc                                                <  1:69781/229‑1 (MQ=255)                                                                                                                                                                                                                                    cAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTCACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGAGATTAGCTCAGCTGGGAGATCACATCCCTTACAAGGAGGGGGTCGGCGGTTCGAGCCCGTCATcaccctccccccgtccactagc               >  1:332055/1‑234 (MQ=38)                                                                                                                                                                                                                                       gTTCGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa            <  1:16115/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGAATCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAAGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGGATCGGGTGATTAGATCAGCTGGGAGAGCACCTCCCTTAAAAGGAGGGGGACGGCGGTTCGAACCCGTCATCACCCACCACCGGGTCggttgcttcgtt         >  1:368240/1‑240 (MQ=38)                                                                                                                                                                                                                                            aaTCCTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGGTCCCGTCATCACCCACCACCGGGGCGTTAGCTCAGtttg       >  1:201137/1‑249 (MQ=255)                                                                                                                                                                                                                                             aTCCTGCACGACGCCCCAATCGCGAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCGTCTCGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTTATAAGCTCAGCTGTGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGAGCCCGTCATCTCCCTCCACCGGGGCGTGAGCGCAGTTGGt      <  1:480336/251‑1 (MQ=25)                                                                                                                                                                                                                                               ccTGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTag    <  1:540487/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tGCACGACTCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGGTCCCGTCATCACCCCCCACCGGGGCGTTTGCTCCGTTGGTaga   >  1:213645/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ggCACGACTCAACAATCGCTAAGTG‑GGAAGCGGTTGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGTGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTagag  <  2:43157/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     AACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTT‑TTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGT‑GGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCAC‑CACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAG  >  NC_000913/780378‑780866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑