| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 721,604 | G→A | R44C (CGT→TGT) | kdpE ← | DNA‑binding transcriptional activator KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 721,604 | 0 | G | A | 97.4% | 229.9 / ‑4.2 | 78 | R44C (CGT→TGT) | kdpE | DNA‑binding transcriptional activator KdpE |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (35/39); total (39/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACG‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACAT > NC_000913/721367‑721845 | gggtggcaggggtgcggcgTAATGCGCCGCGAAGACGGGCCTGCAATTCGCCAATGCCGAACGGCTTACGCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGTCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTcc < 2:624436/238‑1 (MQ=255) gtggCAGAGTGGCGGCGTAATGCGACGCGCAGACTGGCCGGCACTTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAg < 2:518571/251‑1 (MQ=255) ggCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCCATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTTTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATAACCGGCACCGCGCCCCACTGGCGCCGGTCGCGGATAAACACAATCCCATCCCCATCCGGCAGGCCGAGATCGGAAATAATCAAATCTGGCTTACC‑GGTTGCCGATTCCatc > 2:365895/1‑248 (MQ=255) gACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCgg > 1:156682/1‑251 (MQ=255) aGACGGTCCTGCTATACTCCAATGCCAAACGGCTTACGCAGATAATCATCCGCGCCTGCAACCAGCGCGGCGA‑TTTTGTCGCTCTCGTCGCGGCGTGTGGAAAGCCCAATCACCGGCACCGCGCTGCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGTGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCaaa < 2:320242/251‑1 (MQ=255) aCGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGa < 1:365895/251‑1 (MQ=255) gggCCTGCACTTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGc > 2:91619/1‑222 (MQ=255) gggCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGc < 1:91619/222‑1 (MQ=255) ggaccTGCACTTCGCAAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGAATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGCAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATAGGGCAGGCCGAGATCGAGACTAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCATTGCAGCGTTTCGGACTCCAAGAcc > 2:339489/4‑250 (MQ=255) tcaaatCGCCAATGCCAAAAGGCTTACTCAGATAAGCACCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGGCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAAGCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTTAAAGACGCGCAt < 2:116987/245‑1 (MQ=255) cAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGACATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATcc > 1:258815/1‑251 (MQ=255) tCGCCAATGCCAAACGGCTTACTCAGATAACCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGt < 2:466358/250‑1 (MQ=255) cGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTTGGCCTCAAAGACGCGCATCCCGTCg < 1:207860/251‑1 (MQ=255) cGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCCCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTc < 2:208178/250‑1 (MQ=255) aTGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGc > 1:318538/1‑205 (MQ=255) aTGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTc > 2:407983/1‑251 (MQ=255) aTGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGCAGCCCTc > 2:14760/1‑251 (MQ=255) ccAAACGGCTTACCCAGATAATCATCCGCGCCTGCATCCAGCGCGGCGA‑TTTTGTCGCTCGCGTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATTAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCTGTGCAGCGTGTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAg < 2:36219/251‑1 (MQ=255) cacaCGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGATCGCGGATAAACTCAATCCCATCCCCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGATGACGCTTCCAGCACGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGcccccc > 2:638806/4‑245 (MQ=255) aCGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGcc < 1:577575/251‑1 (MQ=255) aTAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCACTCTTCGCTGCGTGCGGCAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCAGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTACAGCGTTTCGGCCCCACAGACGCGACTCCCGCCGCCCTCCAGCGccagtcgcaaaaaac > 2:657494/1‑238 (MQ=255) aTAATCAGCCGCTCCGCCATCCAGCGCGGCGAGTTTTG‑CGCTCTCTCCGAGGCATGCGGAAAGCACAAGCACCGGCACAGCGCTCCACTGGCGCAGGTTGCGGATAAACTTAATCCCACCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTTCCGCTGCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGACGACCTGCAGCGCCGTGCGCAGAAAg < 2:670170/250‑1 (MQ=255) atcCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCCGAAAGcgacga > 2:411984/1‑250 (MQ=255) cGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGACAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGCGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCCAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAgcacgcgcagaca > 2:173172/1‑237 (MQ=255) gCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGc > 1:209987/1‑251 (MQ=255) gCTCCGACATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGCAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGCTAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCACAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGcgacggctagc > 2:429992/1‑245 (MQ=255) cTCCGGCATCCAGCGCGGCGA‑TATTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGCCGCGCATCCCGTCGCCCTCCAgcgcagcgagcagacatcgccgtatagcc > 2:101711/1‑226 (MQ=255) gCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTc > 1:157459/1‑251 (MQ=255) cATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGTGCATAAAGCGACGAATAGCCTGTTCa < 2:93434/251‑1 (MQ=255) ggcggggcgA‑TTTTGTCGCTCTCTGCGCGGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTc < 2:477238/246‑1 (MQ=255) gcgcGGCGA‑TTTTGTCGCGCTCTTCGCTTCGTGCGGAAAGCACAATCACCGTCACCGCGCTCCACTGGCGCAGGGCGCTGATAAACTCAATCCCATCACCATCGGGCAGGCCGGGATCGAGAATAATCAAAGCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCGCCAGCGCCGTGCGCGGAAAGTGACGAATAGCCTGTTCATCTTca < 2:30865/251‑1 (MQ=255) gcggcgA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGCGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAACGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTAATCTTcaac > 2:143726/1‑251 (MQ=255) gcggcgA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTAGCGGATAAACTCAATACCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCATCAAAGACGCGCATCCCGTCGCCCTCCAGCGACGTGCGCAGAAAGCGCCGAATAGCCTGTTCATCTTcaac > 2:443661/1‑251 (MQ=255) gcgA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAAt < 1:51313/251‑1 (MQ=255) gcgA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAAt < 1:630983/251‑1 (MQ=255) gcgA‑TTTTGTCGCTCTCTTCGCTGCGGGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAAt < 1:429397/251‑1 (MQ=255) gA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATc > 1:19214/1‑250 (MQ=255) a‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATc > 1:201405/1‑249 (MQ=255) tttGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGa < 1:411984/250‑1 (MQ=255) ttgtagccctctTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCACGCCGCGTTGCAGCGTTTCGGCCTCAAAGTCGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAAc > 2:662734/9‑251 (MQ=255) ttGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGaa < 1:662848/250‑1 (MQ=255) ccctTCGCGGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACGGGCGCAGGTCGCGGATAAACTCAATCCCATCACCAGCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTca < 2:213976/249‑1 (MQ=255) tCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCCCTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAAGCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACat < 2:615451/250‑1 (MQ=255) tCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTcaca < 1:173172/249‑1 (MQ=255) cGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACata < 1:14760/250‑1 (MQ=255) gcgGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGa < 1:194822/251‑1 (MQ=255) aaaGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCACATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAAtt > 1:278327/1‑251 (MQ=255) aaaGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAAtt < 1:501410/251‑1 (MQ=255) acaATCACCGGCACAGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAAttcttc > 1:509259/1‑250 (MQ=255) cACCGGCACCGCTCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCTCCATCGGGCAGGTCGAGATCGAGAATATTCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGGGCGCAGAAAGCGACGAATAGCCTTTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCGTCAAGtt < 2:256646/250‑1 (MQ=255) aCCGGCACCGCGCTCCACTGGCGCAGGTCGCGTATAAACTCAACCCCAACAACATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCATCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCa < 2:135620/251‑1 (MQ=255) gCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCCCCATCGGGCAGGCCGAGATCGAGAATAATCAAAGCGGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAgggg < 2:498249/251‑1 (MQ=255) cgctctccacgtgcgcAGTTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACTTTTGTCACATAGCCTCATGAAATTCTTCAAGTTCTGGGGCAGt < 2:415719/239‑1 (MQ=255) cgcTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGttt > 1:58797/1‑251 (MQ=255) gcTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCt > 1:20020/1‑182 (MQ=255) gcTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCt < 2:20020/182‑1 (MQ=255) gcTCCACGGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCGCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTg < 2:437733/251‑1 (MQ=255) tGGCGCAGGTCGCGGATCAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGAGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACCATCCGaaag > 2:405981/1‑196 (MQ=255) tGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACg < 1:405981/198‑1 (MQ=255) aCTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCCTCTTCAACAATCAGAACGTTTGTCACATCTCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGACGTGTAACACGAAAACAgg > 2:3712/1‑251 (MQ=255) ccAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGca < 1:339489/250‑1 (MQ=255) ccAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGCCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGca < 1:398735/250‑1 (MQ=255) aTCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGcacc > 1:239247/1‑250 (MQ=255) aTCCCAGCACCCTCGGGCCGGCCGATATCGAGAATAATCAACTCTGGCTTACA‑GCTGGCCGCTTCCAGCACGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTGGCTGGGGAAGTGTAACACGAAAACAGGctcc‑a < 2:514822/251‑1 (MQ=255) ccATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCt > 1:346400/1‑251 (MQ=255) tCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACG‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAATAAGGCAAC‑ACCTTcc > 2:204446/1‑251 (MQ=255) ggCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTCCGGTCGGTTGAAcg < 1:527111/251‑1 (MQ=255) gCCGAGATCGAGAATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTCAGGTCGGTTGAAcgg > 2:513496/1‑250 (MQ=255) accGAGATCGAGAATCATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGAAAGCCGCGTTGCAGCGTTTCGGCATCACAGACGCGCATCCCGTCGCCCTCCCGCGCCGAGCGCAGAAAGCGACGAATAGACAGATCATCTTCAACAATCAGACCGTTTGTCACATATCCTCATGAAATTGTTCAAGGTCAGGTGCCGCTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTccagtagattggacgg > 2:513503/2‑237 (MQ=255) gaTCGAGAATAACTAAATCTGGCTTACA‑GATTGCCGCTTCCAGAAAGACGCGTTAAAGCGTTTAGGCATCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAACGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTGACATATCCTCATGACATTCTTCACATTCACGTACAGATTTCTGGATAAGGGTAACACCAAAAC‑CACACCCACCTTCAGGTCGGTTGAACGCgggat > 2:235736/1‑248 (MQ=255) gagaATAATCTACTCTGGCTTAAA‑GGTTGCCGCGTCCAGCAAGCCGCGTTGCTGCATTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCTTGCGTAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCATATATCCTCATGAAATTCTTCAAGTGCAgg < 2:213003/182‑1 (MQ=255) gagaATAATCAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCCGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAAAGTTTGTCGCATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTCCGGTCGGTTGAACGCGGTAATAGt > 1:52941/1‑251 (MQ=255) gagaATAATCAAATCTGGCTTACA‑GGATGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGGAAGCGCCGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAgg > 1:213003/1‑182 (MQ=255) atCAAATCTGGCTTACG‑GGTTGCCGCTTCCAGCAAGCAGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGGGCGCAGAAAACGACGAATAGCCTGTTCATCTTCAACAATAAGAACGTGTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGTAAGTGTAACACGAAAACAGGCACC‑ACCTTCCGGTCGGTTGAACGCGGTAATAGTGACCCCg > 2:369407/1‑251 (MQ=255) atCAAATCTGGAATACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTAGCAAACATCCTCATGAAATTCTTACAGTTCAGGGACAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCCAC‑AACTGACGGTCGGTTGAACGCGGTAATAGTGCCCCCg > 2:411840/1‑251 (MQ=255) cAAATCTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGTCGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAACTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCgt < 1:638806/250‑1 (MQ=255) acaTCTGGCTTACCCGGTT‑CCGCTTCCAGCAAGCCGCGGTGCCGCGTATCTGCCTCCAAAACGCGACTCCCGTCAACCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCCACAATCAGAACGTTTGTCACATATCCTCATGCAATTCTTAAAGTTCAGGGGAAGGTTGCTGGGGAAGGGCAAAACGGAAAAAGGCACC‑ACCTTCCGGCCGTTTGAACGCGTTAATAGTGCCCCCgggt > 2:107133/3‑248 (MQ=255) cTGGCTTACA‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGACATTCTTCACGTTCTGGGGCAGTTTGCTGGGGCAGTGTAACACGAAACCAGGCACC‑ACCTTCCGGTCGGTTGCACGCGGAACTAGTACACCCGTGTACAt > 2:220263/1‑251 (MQ=255) | TGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGA‑TTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACG‑GGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACC‑ACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACAT > NC_000913/721367‑721845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |