Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 694,941 G→A L5L (CTC→CTT)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913694,9410GA95.7% 131.5 / ‑1.6 47L5L (CTC→CTT) miaBisopentenyl‑adenosine A37 tRNA methylthiolase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (24/20);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

GCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGC  >  NC_000913/694697‑695174
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         
gCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGtttt                                                                                                                                                                                                                                     <  1:190934/251‑1 (MQ=255)
gCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGtttt                                                                                                                                                                                                                                     <  1:42715/251‑1 (MQ=255)
 cGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGttttt                                                                                                                                                                                                                                    <  1:421637/251‑1 (MQ=255)
       cccGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCTATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCa                                                                                                                                                                                                                              <  2:235592/249‑1 (MQ=255)
                   gACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGCCAGCCCCAGGTTTTAATATGTAGTTTTTTGGTCATCGACTTGctct                                                                                                                                                                                                                  >  2:18446/1‑251 (MQ=255)
                    aCGCCGATAATCAGGTCTGGATTTTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCGTCTCGCGGATTTAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCtt                                                                                                                                                                                                                 <  2:201859/251‑1 (MQ=255)
                       ccGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGAGGATTGAGCCGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGCTGAATCGTCCTCGTTCATCTGACAGCCCCAGGTTTTCATATGAAGTTTTTTGTTCATCGACTTGCTCTTGCg                                                                                                                                                                                                              >  1:625884/1‑251 (MQ=255)
                           tAATCAGGTCTGGATTATTCTCTTTTAACAGAGTCCAGCGACCAAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGCTTGAGCAGGTGTTCAGCAGCAGCACATCCGATTCTTCCGCAACGTCGGTAAGTTGATAGCCGTGGGGGGCATCACGCAGATCGACCATCTTCGATGAATCGTACTCGTTCAACTGACAGCCCCAGGTTTTAATATGAAGTATTTTGGTCATCACCCTGCTCTTTCGaat                                                                                                                                                                                                           >  2:594101/1‑249 (MQ=255)
                                 ggTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGc                                                                                                                                                                                                    <  1:571515/251‑1 (MQ=255)
                                      ggATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAgg                                                                                                                                                                                                >  1:433382/1‑250 (MQ=255)
                                                        aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAgtgt                                                                                                                                                                             >  2:135426/1‑251 (MQ=255)
                                                            tCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAAt                                                                                                                                                                          <  1:182274/250‑1 (MQ=255)
                                                                              ggAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGAc                                                                                                                                                       >  2:576405/1‑251 (MQ=255)
                                                                                     tttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGttttt                                                                                                                                                                                                                                    >  1:342191/1‑167 (MQ=255)
                                                                                     tttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGttttt                                                                                                                                                                                                                                    <  2:342191/167‑1 (MQ=255)
                                                                                                        cgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAgg                                                                                                                             <  1:4590/251‑1 (MQ=255)
                                                                                                         ggATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAggg                                                                                                                            <  1:576405/251‑1 (MQ=255)
                                                                                                                     tgtgaagcagcagcACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCTTCGACTTTCTCTTGCGACATAGTAGCCAGGAATGCAGGGCGCATAGGGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCATCCCTTAGGGGTAAAAATcatg                                                                                                                >  2:168371/6‑248 (MQ=255)
                                                                                                                     gttgcagcactgtcacaTCCGCTTACTCCGCCACGTCGGTCATGTGATATCCGTGGGGTGCCTGCAGCAGCACGGCAATCTTCGATGAATCGTACTCGTTCATCTTACAGCCCCAGGTTTTAATATGAAGTTTTTTGGGCACAGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGTTTTGCTGCCGTTGTTACCAGTATGGGCGCTATCAGCACTTAGGGGTTAAAAGCCTg                                                                                                                <  2:347166/238‑1 (MQ=255)
                                                                                                                       ttctgcagaaacacaGCCGCTTTTTCAGCCACGTCGGTCAGTTGATAGCCGTGGTTGGCATCCAGCAGATCGGCAATCTTCGATTATTTGTACTCTTTCATCTGACAGCCCAAGGTTTTAATATGAAGTTTGTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCTGCTCTTAGGTGTAAAATTCCTGt                                                                                                               <  2:463353/239‑1 (MQ=255)
                                                                                                                          agcagcagcACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAAc                                                                                                           <  1:135426/251‑1 (MQ=255)
                                                                                                                             agcagcaCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTa                                                                                                        <  1:117388/251‑1 (MQ=255)
                                                                                                                               cagcaCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGACGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTaa                                                                                                       >  1:589616/1‑250 (MQ=255)
                                                                                                                                agcaCATCCGCTACTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCtt                                                                                                                                                                      >  1:317070/1‑186 (MQ=255)
                                                                                                                                agcaCATCCGCTACTGCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCtt                                                                                                                                                                      <  2:317070/186‑1 (MQ=255)
                                                                                                                                     aTCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCatat                                                                                                >  1:266975/1‑251 (MQ=255)
                                                                                                                                               tcCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAAc                                                                                         <  1:511788/248‑1 (MQ=255)
                                                                                                                                                      cGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTg                                                                               <  1:638699/251‑1 (MQ=255)
                                                                                                                                                          ggTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGAcc                                                                            >  1:478225/1‑250 (MQ=255)
                                                                                                                                                          ggTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGAcc                                                                            >  1:241692/1‑250 (MQ=255)
                                                                                                                                                           gTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATAGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCAt                                                                          >  1:655667/1‑251 (MQ=255)
                                                                                                                                                              aGTTGATAGCCGTGGGTGGCATCCAGAAGATCGGCCATCTTCGATGAATCGAACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGATTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATACTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACAATGAc                                                                       >  2:255062/1‑251 (MQ=255)
                                                                                                                                                                   aTAGCAGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTCATATGATGTTTTTTGGTAATCGCCTTGCTCTTGCGTAATAGTAGCCAGGAATGCAGGGCGCATAGTGTATTGCTTTGCTGCCGTTGTGACCCGTATGAGCGTTATCAGCCCTTCGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATc                                                                  >  1:91843/1‑251 (MQ=255)
                                                                                                                                                                    tAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGCCTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcc                                                                 >  2:297091/1‑250 (MQ=255)
                                                                                                                                                                        cGTGGGAGGCATCCAGCAGATCGGCCATCGTCGATGAAACGTACTCGTTCATCTGACAGCCCCAGGATTTAATATGAAGTTTTTTGGTCATCGACTTGCTATTGCGAAATAGTAGCAAGGAATGCAGGGCGCATAGTGTAATGATTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCATGTAAACTTAAAGCATATTGCTAATCGGATGATGGACAATGACAAATaaacc                                                              >  2:395584/1‑250 (MQ=255)
                                                                                                                                                                         gtgggtggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcaaccaa                                                            <  1:37910/251‑1 (MQ=255)
                                                                                                                                                                               ggcatccggtatttCGGCCATCATCGATGAAGCGGACGCGTGTATCTGACAGTCCCAGGTTGTAATTTGAAGTTTTTTGGTCATCTACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGTCCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAACCTTCAAGCATATTGCTAACAGTATGATTGACCATGACAAATCAACCAACGGaaa                                                      <  2:151062/238‑1 (MQ=255)
                                                                                                                                                                                gCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGATTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATAAACCAACGGacat                                                     >  2:541417/1‑248 (MQ=255)
                                                                                                                                                                                           aTCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCAAATTGCTAAAAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcg                                          >  2:482255/1‑251 (MQ=255)
                                                                                                                                                                                             cGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTACTATGAAGTTTTTTGGTCATCGCCTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcggc                                        >  2:466630/1‑251 (MQ=255)
                                                                                                                                                                                              ggCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcggcg                                       >  1:43772/1‑251 (MQ=255)
                                                                                                                                                                                                    catCGATGAATCGTACCCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTGTTTGGGCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGACGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGAGTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCggagga                                  <  2:433382/248‑1 (MQ=255)
                                                                                                                                                                                                       cGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATgg                              <  1:72004/251‑1 (MQ=255)
                                                                                                                                                                                                        gATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGt                             >  1:128342/1‑251 (MQ=255)
                                                                                                                                                                                                                         cATCTGACAGCCCCATGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGgcgc            >  1:68613/1‑251 (MQ=255)
                                                                                                                                                                                                                              gACAGCCCCAGGTTTTACTATGAAGCTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTgggg       >  1:218764/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ccccAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTGAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGGGGAATGGTCCGCGGCGCCCTGGCGCTAGGGCTGGc  >  1:494953/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         
GCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGC  >  NC_000913/694697‑695174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑