Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,742	G→A	G276D (GGT→GAT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,742	0	G	A	97.4%	234.1 / NA	78	G276D (GGT→GAT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/38);  minor base C (0/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGC  >  NC_000913/534496‑534986                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       aaaaaGCTGTAGCAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGGG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATc                                                                                                                                                                                                                                                    >  2:205086/1‑251 (MQ=255) aaaaaGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATc                                                                                                                                                                                                                                                    <  1:256236/251‑1 (MQ=255) aaaaaGCGGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCCGACTCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGGGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCTCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGTCATGGTGTTTGATATc                                                                                                                                                                                                                                                    <  2:65087/251‑1 (MQ=255) aaaaaGATGTCGAAATGTTAATCCAGGCCGCACGTCCGGTGATTGTTGCCGGGGGAGGAGGAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGGTCCGGTGAT‑CCCACCGCTAATGGGCGGGGGCTGTATCCCGGACGTTCCTGAACCAATGGCCGGGTTGGTG‑GGGCTGCCAACCACGCCTCGTTATGGAACCGAAACGCTGCTGGCTTCGGTAATGGTGTTTGATATc                                                                                                                                                                                                                                                    >  2:545608/1‑251 (MQ=255)           aGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGGG‑GGTCTGCAAACCGAGCATAGTTACGGGAACGCAACGCTGCGGGCGTTTGACATGGGGTTTGATATCGGGAACCgat                                                                                                                                                                                                                                          >  1:562353/1‑249 (MQ=255)                gtttAAGCCAGGCCGGACGTCCGGTGATTGGGGCCTGGGGGGGGGTAACTAATGCTGACCCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGG‑GTTGCCCACCGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACAGATGGCCGGAATGGTG‑GGGCGGCAAACCGCGCATCGTTACGGTAACGCAACGCCGCTGGCGTCGGACATGGTGTTTGATATCGGTAACCGTTTTGcg                                                                                                                                                                                                                                     <  2:528578/249‑2 (MQ=255)                    aaTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAAcc                                                                                                                                                                                                                                 >  1:256141/1‑251 (MQ=255)                       ccAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTc                                                                                                                                                                                                                              >  1:510831/1‑251 (MQ=255)                          ggCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGCAGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATa                                                                                                                                                                                                                           <  1:447571/251‑1 (MQ=255)                              gcaCGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCCAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                                                                                                                                                       >  1:549948/3‑251 (MQ=255)                              gAACGTCCGGTGCTTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                                                                                                                                                       >  1:549943/1‑251 (MQ=255)                              gAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCgg                                                                                                                                                                                                                       >  1:395628/1‑251 (MQ=255)                                      ggTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAAAAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGAGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCggc                                                                                                                                                                                                                 >  1:351575/1‑248 (MQ=255)                                       gTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAAATGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGCTTTGCTCACCGTCATACCGGCTCGGt                                                                                                                                                                                                                 >  2:636817/1‑248 (MQ=255)                                         gATTGTGGGCGGGGGCGTGGTAATTAATGCTGACCTAGATTCACTGTGACAGCAGTTTGATGAACTTATCAGCGTTCCGGTGAT‑CGCAACGCTAAGGGGAGTGTGCTGGATCCCGGACGATCATGAACTGATGGTCGGGATGGTG‑GGGCTGCAATACGCGCATCGTTACGGTATCGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTATCCGTCATACCGTCTCTGTTgaga                                                                                                                                                                                                            <  2:35258/251‑1 (MQ=255)                                                gtcGGGGGCGGTGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCATCGTTCCGGTGAT‑CCCAACTCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGGCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTTCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATaca                                                                                                                                                                                                      <  1:92385/248‑1 (MQ=255)                                                gcggggggCGGGGTAATTAATGATGACGCAGCGGCACTGTTACAACAGTTTGCTGAACTGAACAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCAGCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATaca                                                                                                                                                                                                      <  1:221073/247‑1 (MQ=255)                                                 ccGGGGGCGGGGTAATTAATGCTGACGCAGCTGCAATGTTACAACAATTTGCTGCACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGCTCCTGAACTGATGGCCGGGGTGGTG‑GGTCTGCAAACCGCGCATCGTTCCGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCCTACCAGCTAGGGagagcaaaatccc                                                                                                                                                                                                    >  2:587570/1‑242 (MQ=255)                                                      ggCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAAATGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGCTCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATCCAACGAAgg                                                                                                                                                                                               >  1:449065/1‑251 (MQ=255)                                                          gagTAATTAATGCTGACGCAGATGCACTGTTACAACAGTTTGCTGAACTGACCAGGGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGCTCATGAACTGATGGCCGGGATGGTG‑GGTCTGAAAACCGCGAATCGTTACGGTAACGCAACGCTGCTGGCGTCTGCCATGGTGTTTGATCTCAGTAACCGTTTTGCTACACGTCATACCGGCTCGGgagaagaaaaaacggcagggggc                                                                                                                                                                                           >  2:77809/3‑232 (MQ=255)                                                                 taatGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATc                                                                                                                                                                                     <  1:2272/250‑1 (MQ=255)                                                                     gCTGACGCAGCTGCACTGTTACAACAGTTTGCTGATCTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCa                                                                                                                                                                                <  1:579791/251‑1 (MQ=255)                                                                      cTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGTGCTGTATCCCGGACGATCATGAACTGCTGGCCGGGATGGTG‑GGTCTGCAAACCTCGCATCGTTCCGGTAACGCAACGCTGCTGGCGTCTGCCATGGGGTTTAATATCGGTAACCGTTTTGCTACCCGTCCTACCGGCTCGGGAGAGAAATaacacgaagggaccaaaaacgctccc                                                                                                                                                                               >  2:266079/1‑226 (MQ=255)                                                                      cTGACGCAGCAGCACTGTTACAACAGTTTGCTGAACTGACAAGCGTTCCGGTGAA‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGGG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCCACGCTGCTGCCGTCTGACCAGGTGTTTTATATCGGTAAACGTTATGATAACTGTCATACAGGATCAGTAGTGAAAtgccacgaaggtcagcaaaccgttca                                                                                                                                                                                >  2:124212/1‑225 (MQ=255)                                                                         gcGCAGCTGCCCTGTTACAACAGTTGTCTGAACTGGCCAGCGTTCCGGTGAG‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatat                                                                                                                                                                             <  2:642698/249‑1 (MQ=255)                                                                         aCGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatatt                                                                                                                                                                            >  1:591780/1‑251 (MQ=255)                                                                           gCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaaa                                                                                                                                                                                        <  1:11896/237‑1 (MQ=255)                                                                           gCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCaaa                                                                                                                                                                                        >  2:11896/1‑237 (MQ=255)                                                                            cAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCatattgat                                                                                                                                                                         >  1:678521/1‑251 (MQ=255)                                                                                     tGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGAAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑g                                                                                                                                                               >  1:660571/1‑251 (MQ=255)                                                                                      gTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGcc                                                                                                                                                                 <  1:120200/249‑1 (MQ=255)                                                                                      gTTACAACAGGTTGCTGAACTGACCAGCGTTCCGGCGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTTACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGcc                                                                                                                                                                 <  1:100063/249‑1 (MQ=255)                                                                                         acaacaGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGc                                                                                                                                                           >  1:189642/1‑251 (MQ=255)                                                                                         acaacaGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGc                                                                                                                                                           >  1:431980/1‑251 (MQ=255)                                                                                              aGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAAtt                                                                                                                                                      >  1:188120/1‑251 (MQ=255)                                                                                               gTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTg                                                                                                                                                     >  1:577658/1‑251 (MQ=255)                                                                                                         cTGACCAGCGTTCCGGTAAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACCCCGAAGGGCGCAAAATCGTTCATATTGATCTTGAGCC‑GCCGCAAATTGGTcgctgtct                                                                                                                                           >  1:310218/1‑246 (MQ=255)                                                                                                           gACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCtg                                                                                                                                          <  1:357428/250‑1 (MQ=255)                                                                                                             ccAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAATGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTgtgt                                                                                                                                       <  1:54143/251‑1 (MQ=255)                                                                                                               cgCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTcc                                                                                                                                     <  2:189642/250‑1 (MQ=255)                                                                                                                 ggTTCCGGTGAT‑CCCAACGCTAAGTGGCTGGGGCTGGATCCCGGCCGATCATGAACTGAGTGCCTGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCgg                                                                                                                                   <  1:292983/250‑1 (MQ=255)                                                                                                                     ccGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGAtct                                                                                                                               <  1:368238/251‑1 (MQ=255)                                                                                                                              cccAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTAtt                                                                                                                        <  1:511945/250‑1 (MQ=255)                                                                                                                                  aCGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCCGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGtctc                                                                                                                   <  1:82992/251‑1 (MQ=255)                                                                                                                                        aTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACg                                                                                                                                                            >  1:495888/1‑204 (MQ=255)                                                                                                                                        aTGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGAGGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACg                                                                                                                                                            <  2:495888/204‑1 (MQ=255)                                                                                                                                            gctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGGG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTaaa                                                                                                        <  1:59071/251‑1 (MQ=255)                                                                                                                                               ggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAgcg                                                                                                     >  1:318019/1‑251 (MQ=255)                                                                                                                                                   ctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAAACGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATCCACCGAAGGGCGCAAAATCGTTCATATTTATATTGAGCCAGA‑GCAAATTGGTAGCGTGCTGTGTCAGGTTCTCGGTATTGTCTCTGTAT‑CTAAAGCGgcgc                                                                                                 >  2:526870/1‑251 (MQ=255)                                                                                                                                                     gTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAAGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTTTCTCTG‑ATGCTAAAACGGCGCTg                                                                                               >  2:672208/1‑251 (MQ=255)                                                                                                                                                       aTCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGac                                                                                             <  1:142261/251‑1 (MQ=255)                                                                                                                                                          ccggccgttcaggAACTGATGGCCGGGATGTTG‑GGTCTGCAAACCGCGCATCGTTTCGGTAACGCAACTCTGCTGGCGTCTGACATGGTGTTTGATATCGGTATCCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAAGTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACAct                                                                                          <  2:240314/239‑1 (MQ=255)                                                                                                                                                           cGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCACACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATAAACCGCAGGGCGCAACATCGTTCCTATTGAAATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGGTCTCGGAATTGTCTCTG‑CTGCTTAAGCGGCGCTGACActg                                                                                         >  2:312569/1‑251 (MQ=255)                                                                                                                                                               cGATCATGAACTGATGGCCGGGAGGGGG‑GGTCTGCAATCCGCTCATCGTTACGTTAACGCAACGCTGCTGGCGTCGGACATGGTGTTTGCTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAAAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGGCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTgg                                                                                     <  2:5330/251‑1 (MQ=255)                                                                                                                                                                 aTCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACAcc                                                                                                                                                                                                    <  1:18590/140‑1 (MQ=255)                                                                                                                                                                 aTCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACAcc                                                                                                                                                                                                    >  2:18590/1‑140 (MQ=255)                                                                                                                                                                          ggATGGCCGGGATG‑TGTGGTCTGAAAACCGCGCAGCGTTACGGTAACGCAACGCTGCTGGCGACTGACATGGTGGTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGATAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCCG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGgcg                                                                          <  1:400253/250‑1 (MQ=255)                                                                                                                                                                                  gggATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTACCCGTCATACCGGCTCGGTAGAGAAATACACCGCCAGGCGCAACATCGTTCATATTGGTATTGAGCA‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGGCTCTG‑ATGCTAAAGCGGCGCTGGCCCTACTGGTTGAAGTGgcgcggg                                                                      >  2:205524/1‑244 (MQ=255)                                                                                                                                                                                  gggATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAgg                                                                      >  1:268829/1‑247 (MQ=255)                                                                                                                                                                                   ggATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGCCACTGCTGGTTGAAGGGGCGCAGGAGATg                                                                 >  1:461277/1‑251 (MQ=255)                                                                                                                                                                                     aTGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGGCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGAGCAGGAGATGc                                                                >  1:608467/1‑250 (MQ=255)                                                                                                                                                                                     aTGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCa                                                               <  1:289954/251‑1 (MQ=255)                                                                                                                                                                                          g‑ggTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCaaaaa                                                           <  1:672208/250‑1 (MQ=255)                                                                                                                                                                                                   actgctctctttgttagcgTAAAGCAACGATGCATGCGGCTGACATGGTGTTTTATATCGGTTACCGTTTTGCTAACTGTCGTACCGGCACGGGAGAGAACTACACCGAAGGGCGCAAAATCGTTCATAGTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGGTGAAGTGGCGCAGGAGATGCACAAAGCGGgttgt                                                  <  2:549948/233‑4 (MQ=255)                                                                                                                                                                                                   aaaCCGCGCAGGGTTACGTTAACGCACCGCTGCTGGCGGCTGACATGGTGTTGGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCTGGATCTCGGTAGTGTCTCTT‑ATGCTAAAGCGGCGCTTACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGttcgt                                                  <  2:549943/251‑1 (MQ=255)                                                                                                                                                                                                      ccGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGACGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTg                                               >  1:196118/1‑251 (MQ=255)                                                                                                                                                                                                        ggtcattgtttcggtgtcgcagcgcTGTTGTCGTCTGTCTTGGTGTTTGCTATCGGTAACCGTTTTGCTAACCGTCATACCGGCGCGGTAGAGAAATACACTGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑TTCTCAAATTTGTCGTGTGCTGTGTCCGGATCTCGGTATTGTTTCTG‑ATGCTAAATCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGTAGATGCAAAAAGCGTGTCTTCTGcc                                             <  2:524661/234‑1 (MQ=255)                                                                                                                                                                                                             tCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGGTATTGAGCC‑GACGCAAATTGGGCGCGTGCTGTGTCCGGATCTCGGAATTGGCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGAGGGTAGTCTGCCGTGTc                                        >  2:551043/1‑251 (MQ=255)                                                                                                                                                                                                                  aCGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTaaa                                   <  1:205086/251‑1 (MQ=255)                                                                                                                                                                                                                   cGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTaa                                    >  2:217096/1‑249 (MQ=255)                                                                                                                                                                                                                     gTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGaa                                <  1:636817/251‑1 (MQ=255)                                                                                                                                                                                                                           caacgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTc                          <  1:217096/251‑1 (MQ=255)                                                                                                                                                                                                                               gctgctGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCg                      <  1:470458/251‑1 (MQ=255)                                                                                                                                                                                                                               gctgctGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCg                      <  1:232932/251‑1 (MQ=255)                                                                                                                                                                                                                                ctgctgGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCg                      >  1:144432/1‑250 (MQ=255)                                                                                                                                                                                                                                  gcgggagTCTGGCATGGTGGTTGATATCGGTAACCGTTTTGCGAACCGTCATACCGGCTCGGTAGAGAAATACCCCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGAc                    <  2:395628/244‑1 (MQ=255)                                                                                                                                                                                                                                                 ggTGTTTGATATCGGTAATCGTTTTACTAACCGGCATACCGTCTCGGTAGATAAATACACCGAAGGGCGCAAAATCGTGCATATTGATAGTGAGCT‑GACGCAAAGTGGTCGCGTGCTGTGTCCGGATCTCGGTAATGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTaaaa    <  2:380492/251‑2 (MQ=255)                                                                                                                                                                                                                                                    gtTTGATATAGGAAACCGTTTTGATAACCGTCATGACGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGTCGCAGGAGATGCAAAAAGCTGGTCGTCTGCCGGGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGc  <  2:318019/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       AAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGAT‑CCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTG‑GGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCC‑GACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTG‑ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGC  >  NC_000913/534496‑534986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑