Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,622	C→T	T168I (ACC→ATC)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,622	0	C	T	95.1%	163.6 / 0.1	63	T168I (ACC→ATC)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (28/30);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

CGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCACCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAGAGTT  >  NC_000913/495380‑495865                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      cgacgaAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAaaga                                                                                                                                                                                                                                                 >  1:255734/1‑249 (MQ=255)    agcaGTGATTGCCCATCTGGGGTCTAGCGCTAAATCCGGTACACAATCATTCCTCGAATCCCTGTGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCATTTTGGTGTTGGTTTCTGCGCTGCGTTTAGCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATCCACCGGTG‑CCGACATCATCAAAGAAGAT‑c                                                                                                                                                                                                                                           <  2:116347/248‑1 (MQ=255)     gaAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAaagaa                                                                                                                                                                                                                                                <  2:227886/246‑1 (MQ=255)     gaAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAaagaa                                                                                                                                                                                                                                                >  1:227886/1‑246 (MQ=255)                 cATCTGGGGACTATCGCTAAATCCGGTACCAATTCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAACACCCAGACCATGGCGTCTTCTGGGAATCGGCTGGCGAAGGAGAATACACCGTTG‑CCGACATCATCAAAGCAGGT‑CGTGG‑TACTGAAAt                                                                                                                                                                                                                             >  1:270331/1‑251 (MQ=255)                    cggggtcccaTCGCTAAATCCGGTACCAAACCAGTCCGCGAATCCCTGGGTGCTGACCATGCGAACGCCAGCCATCTGATCGGCCAGTTGTGTGGTGTGTTCTACCCTGCGTTTATCGTGGCCGACAAAGTGACCGGGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAACACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACGGaaagca                                                                                                                                                                                                                           <  2:270331/241‑4 (MQ=255)                    cTGGGGACAATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACACAGTGACCGTGCGTACTCGAGCGGCAGGCGAAAAACCAGCAAATGGCGTCTTCTGGGAATCGGCTGGAGAAGGGGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑caggggaatgaaataac                                                                                                                                                                                                                           >  2:474909/1‑235 (MQ=255)                       ggggcTATCGCTAAATCCGTTACCAAATCCTTCCTCGAATCCCTGGGTTCTGCCCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCCGGCGTAAAACCAGACAATGGCTTCTTCTGGTCGTCTGCTGTCGCCGGAGAATCCCCCGTTT‑CCGACCTCATCCAAGAA‑ATGCGTGG‑TACTGCAATCACCCt                                                                                                                                                                                                                       >  2:272336/5‑251 (MQ=255)                       gggACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGCCCAGGCGAAAGACAGACAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGAAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGCATCGGCTGGCGCAAGTGAATACACCGTTG‑CCCACATCATCACAGACGGT‑‑GTGGGTACTGAAATCAcccc                                                                                                                                                                                                                       >  2:41618/1‑250 (MQ=255)                                   aaaTCCGGTCCCAAATCATTCCGCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTTATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGGTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCTCCCTGCATCTGCGTGa                                                                                                                                                                                                           <  2:228670/251‑1 (MQ=255)                                             ccAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGAc                                                                                                                                                                                                 <  1:601480/251‑1 (MQ=255)                                              cAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATACACCGTTG‑CCGACATCCTCAAAGAAGAT‑AGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACg                                                                                                                                                                                                >  1:639876/1‑251 (MQ=255)                                               aaaTCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACg                                                                                                                                                                                                >  1:302055/1‑250 (MQ=255)                                               aaaTCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACg                                                                                                                                                                                                >  1:631651/1‑250 (MQ=255)                                                    atgccTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTcc                                                                                                                                                                                          <  1:352151/248‑1 (MQ=255)                                                            aaTCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGAc                                                                                                                                                                                  <  1:150009/251‑1 (MQ=255)                                                               cccTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCTGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTg                                                                                                                                                                                <  2:631651/250‑1 (MQ=255)                                                               cccTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTg                                                                                                                                                                                <  1:41618/250‑1 (MQ=255)                                                                                     aGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCACCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCAATGACTGGCGCGTGCGTTCCATCATCAGCa                                                                                                                                                         <  1:145073/251‑1 (MQ=255)                                                                                       aCAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCaaa                                                                                                                                                       <  1:164146/251‑1 (MQ=255)                                                                                        cagccagcTGATCGGTTAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAAt                                                                                                                                                      <  1:370418/251‑1 (MQ=255)                                                                                        cagccagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAAt                                                                                                                                                      >  1:347272/1‑251 (MQ=255)                                                                                         agccagcTGATCGGTCAGTTTGGTGTTGGTTACTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCTGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATa                                                                                                                                                     <  2:161772/251‑1 (MQ=255)                                                                                             agcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTcc                                                                                                                                                 <  1:38497/251‑1 (MQ=255)                                                                                               cTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCg                                                                                                                                                >  1:618541/1‑250 (MQ=255)                                                                                                  aTCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGTCGAAAACCCAGAAAATGGCGTCTTCTGGAAATCGGCTGGCGAAGGTGAATACAACGGTG‑CCGACATCATCAAAGAACAT‑CGTGG‑TACTGAAATCACCCAGCCTCTGCGTGAAGGCGGAGACGAGTTCCTCGATTACTGGCGGGTTCGTTCCAACATAAGCAAATACTcctgccc                                                                                                                                            >  2:163220/1‑246 (MQ=255)                                                                                                     ggTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCatat                                                                                                                                         <  2:255734/251‑1 (MQ=255)                                                                                                      gTCAGTTTGGTGTTGGTTTCGCCTCTGCGGTTAGCGTGGCCGACAATGTTACCGTGAGTAGTCGCGCGGTAGGAGAAAATCCAGAAAATGGAGTTTGCGGGGAATCGGCTGGCGAATGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAG‑CGTGG‑TACTGAAATCATCCTGTATCTTCGTGAAGGCTAAGACGAGTTCCTCGATGACTGGCGCGTGCGGGCCATCAGAAGCATATACGCCGattatatc                                                                                                                                        <  2:402305/251‑8 (MQ=255)                                                                                                           tttGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGGTG‑CCGGCATTATCAAAGAAGAT‑CGGGG‑TAATGAAATCACCCTGCATCTGCGTGAAGGCGAAAACGAGTTCCTCGATGACCGGCGCGTGCGTTCCATCCTCAGGCAATAATCCGACCataacccgcc                                                                                                                                   >  2:21011/1‑244 (MQ=255)                                                                                                             tGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGc                                                                                                                                 >  1:205046/1‑251 (MQ=255)                                                                                                                     gTTTCTACTCTGCGTTTATCGTTGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTagag                                                                                                                         <  2:250499/251‑1 (MQ=255)                                                                                                                           aCTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACGCCGACCATATCGCGCTGCCGGTAGAGATc                                                                                                                      <  1:137607/248‑1 (MQ=255)                                                                                                                                gCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAAc                                                                                                               <  1:347579/250‑1 (MQ=255)                                                                                                                                   tttATCGTGGCCGACAAAGTGACCGTTCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATACACCGTTG‑CCGACATCATCAAAAAAGAT‑CGGGG‑TAATGACAACACCCCGCATCAGCGTGAAGGCGAAAACGAGTTACGCGATGACATGCGCGGGCGTTCCCACATCAGAAAATAATACGCCCATATCACGCTGCCggcggtgagcgaaaaacacg                                                                                                            >  2:7041/1‑232 (MQ=255)                                                                                                                                    tgaTCGTGGACGACAAAGTGACCGGGCGTACCCGCGCGGCAGGCGAAAAACCAGAAAATGGTGGCCTCTGAGAATCGGATGGCGATTGTTAATACACC‑TTGTCCGACATCAGCAAAGAAGAT‑CGTGG‑TACTGAAATCCCCCCTCATCTGCGTGCAGGCGTAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACGCCGACCATATCGCGCTGCCGGTTGAGATCGtaaatcttga                                                                                                           <  2:6624/248‑7 (MQ=255)                                                                                                                                     tATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGcc                                                                                                                                <  2:291039/228‑1 (MQ=255)                                                                                                                                     tATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGcc                                                                                                                                >  1:291039/1‑228 (MQ=255)                                                                                                                                      aTCGTGGCCGACAAAGTGACCGTGCGTCCTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGCGATCGGAAAACGCGa                                                                                                           >  1:163358/1‑248 (MQ=255)                                                                                                                                        cGTGGCCGACAAAGTGACCGTGCGTACTCGTGCGGCAGGCGAAAAACCAGAAAATGGAGTATTGTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTACTCGATGACTGGCGCGTGCGTTACATCATCAGCAAATACTCCGACCATAGCGCGCTGCCGGTAGAGATCGAAAAACGCGAagag                                                                                                       <  1:102769/250‑1 (MQ=255)                                                                                                                                          gcgctgaaaaaGTGCCCGTGTGTGCTCGCGCGGCAGGCTAAAAAACAGAAAAGGGCGTCTGCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAA‑CGTGG‑TACTGAAATCTCCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACGGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATAGCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGaaa                                                                                                    <  2:400545/243‑1 (MQ=255)                                                                                                                                            gCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGAGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGaa                                                                                                     >  1:606931/1‑248 (MQ=255)                                                                                                                                                               gTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGCGAATAAACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TAATGAAATCAACCTGCATCTGCGTGGAGGCGTAGACGAGTTCCTCGGTGACTGGCGAGTGCGTTCCATCATCGGCAAATACTCCGACCATTTCGCGCCGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAACCCGTTaccacc                                                                               >  2:334159/1‑246 (MQ=255)                                                                                                                                                                 acgcgcgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCt                                                                              <  1:71642/247‑1 (MQ=255)                                                                                                                                                                 aCTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTg                                                                             <  2:347272/251‑1 (MQ=255)                                                                                                                                                                      cgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTggg                                                                           >  1:604477/1‑248 (MQ=255)                                                                                                                                                                      cgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTggg                                                                           >  1:495263/1‑248 (MQ=255)                                                                                                                                                                      cgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGgaga                                                                        <  1:341030/251‑1 (MQ=255)                                                                                                                                                                           cAGGCGAAAAACAAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGaaagtc                                                                   >  1:210660/1‑248 (MQ=255)                                                                                                                                                                                   aaaCCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAAACGGCGAAACCGTTATCTCCTGGGGGAAAATCAACaaaac                                                           >  2:195413/1‑249 (MQ=255)                                                                                                                                                                                         gAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGgc                                                     <  1:487128/251‑1 (MQ=255)                                                                                                                                                                                          aaaaTGGCGTCTTCTGGGAATCGGCTGGCCAAGGTGAATAAACCGTTG‑CCGACATCATAAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGCGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGgcg                                                    >  1:241706/1‑251 (MQ=255)                                                                                                                                                                                            aattgCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGc                                                                                                                                 <  2:395941/168‑1 (MQ=255)                                                                                                                                                                                            aaTGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGc                                                                                                                                 >  1:395941/1‑172 (MQ=255)                                                                                                                                                                                              tgggtttatctgggatgcggatgctgAAGGTGAATCCTCCGTTG‑CCGCCATCATCAAATAAGAT‑CGTGG‑TACTTAAATCCCCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATCCTCCGACCATATCGCGCTTCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAACTCACCAAAGCGCGGTCGCtg                                                 <  2:654768/225‑1 (MQ=255)                                                                                                                                                                                                        tGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCAGCATCAGCAAATACTCCGACCACATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTcc                                                                               <  1:460837/210‑1 (MQ=255)                                                                                                                                                                                                         gcgAATCGGCTGGCGAAGGTAAATACACCGTTG‑CCGTCATCATCAAAGAAGAT‑CGCGG‑GACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGCCGAGTTCCTCGATGACTGGCGCGTTCGTTCCATCCACAACAAATACTCCGACCCTTTCGCGCTGAAGGTAGAACTCGAAAAACGCGTAGAGAAAGACGGCGAAAACGTTATCGCCTGGGAGACGATCAACAAAGCGCAGACGCTGTGGACTCGTAAc                                     >  2:488802/3‑251 (MQ=255)                                                                                                                                                                                                                             gAATACACCGTTG‑CCGACATCACCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGAGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAACGACGGCGAAACCGTTATCTCCTGGGAGAAACTCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATg                  >  2:241749/1‑250 (MQ=255)                                                                                                                                                                                                                                  cacCGTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAag               >  1:162411/1‑248 (MQ=255)                                                                                                                                                                                                                                     cGTTG‑CCGACATCACCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAGTACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTAc          >  1:204266/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTTG‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACa         >  2:141187/1‑250 (MQ=255)                                                                                                                                                                                                                                         g‑CCGACATCATCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAag      <  1:195413/250‑1 (MQ=255)                                                                                                                                                                                                                                            cGACATCATCAAAGAAGAT‑CTTGG‑TACTGAAATCACCCTGCATCTGCGGGAAGTCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAGAGt   <  1:47871/251‑1 (MQ=255)                                                                                                                                                                                                                                             gACATCAGCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCGCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGATGAGTACAAAGAGtt  <  1:450185/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      CGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTG‑CCGACATCACCAAAGAAGAT‑CGTGG‑TACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAGAGTT  >  NC_000913/495380‑495865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑