Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,260	A→T	intergenic (+179/‑348)	rclR → / → ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,260	0	A	T	100.0%	79.1 / NA	28	intergenic (+179/‑348)	rclR/ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/14);  total (14/14)

TTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTT  >  NC_000913/321017‑321506                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        gtGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTGGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACGATTTCTTGAt                                                                                                                                                                                                                                                   <  2:571503/249‑1 (MQ=255)                                                                       aCCGGCAGAAATCAGGGCAAAGATAAGCCGCATTCCGGGAGTTGTTAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGctc                                                                                                                                                                            <  2:642524/251‑1 (MQ=255)                                                                                aaTCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAA‑TGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGt                                                                                                                                                                   >  1:593224/1‑250 (MQ=255)                                                                                    aGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGtata                                                                                                                                                                >  1:23007/1‑250 (MQ=255)                                                                                                  ccGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCAtt                                                                                                                                                <  1:30181/251‑1 (MQ=255)                                                                                                  ccGCAGTCCGGGAGTTGTGGGCCTTTCATCTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCAtt                                                                                                                                                <  2:191372/251‑1 (MQ=255)                                                                                                        tCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTaa                                                                                                                                           <  1:549177/250‑1 (MQ=255)                                                                                                         ccGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAAt                                                                                                                                         >  1:477593/1‑251 (MQ=255)                                                                                                                             aaCTATATCTATTTTCAACGGTGGTTTGGGCTTTATATATTTTTTTTGATGGACTCTAGCTCAGTTTTTAAATATAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTTTTGTCGGTGATTTTTTTGTACACCTTCAGTGTATATTTCACTAACCCCTGAGTAAGTTGATATGAATCGG‑ATAAAGGGATGTCGCCATTATTCAATGGGTTTCTTATGCCAAAAAt                                                                                                                     >  2:249641/1‑251 (MQ=255)                                                                                                                                    tctatttcCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAtcgtc                                                                                                              >  1:565430/1‑251 (MQ=255)                                                                                                                                     atatttcGAGCGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGGTTTTCAATAACAATGCAAACTAACTTGCTTCAATTAAATAAAATCCCGCCTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTATACACAATCAGTGTATATCTCAATAACCCCTGAATAAGTCGCTCTGAATAGGTATAA‑GGACTGAAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGTtcgt                                                                                                               >  2:385474/2‑249 (MQ=255)                                                                                                                                       atttcCAACTGTGTTTTGGGCTTTATATATTGTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAAGTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACATGATTATTGTCGTTTATTTTTTTGTACACCATCATTGTATATCTCAATAACCCCTGAATAAGTAGCTCTTGATAGGTAGAA‑GGGATGTAGGCATTTTTAAGTGGATTTCTTATGCCCAAAATGATCGTCGa                                                                                                            <  2:318342/250‑1 (MQ=255)                                                                                                                                                        gggCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCGTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGa                                                                                          <  2:245895/251‑1 (MQ=255)                                                                                                                                                          gCTTTATATTTTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTGTTGTACATCATCAGTGTATATCTCAATAACCCCTGAATTAGTATCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTGAAATGGATTTCTTATCCCAAAAATGATCGTCGATTTCCCCATAAATTGTGatc                                                                                        <  2:433664/251‑3 (MQ=255)                                                                                                                                                                                    ctAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGAATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGttt                                                              >  1:538988/1‑250 (MQ=255)                                                                                                                                                                                    atAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATAAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCAGAAAGAAATAAAATTAGtt                                                               >  1:538984/2‑250 (MQ=255)                                                                                                                                                                                      aGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGGTTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTAt                                                             <  1:355031/250‑1 (MQ=255)                                                                                                                                                                                          cAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCAt                                                        <  1:507797/251‑1 (MQ=255)                                                                                                                                                                                                    ataaaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGc                                                                                   >  1:285224/1‑214 (MQ=255)                                                                                                                                                                                                    ataaaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGc                                                                                   <  2:285224/214‑1 (MQ=255)                                                                                                                                                                                                     taaaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTgg                                             >  2:187222/1‑251 (MQ=255)                                                                                                                                                                                                       aaaaTGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATTCGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GTGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAGCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGAt                                           <  1:86204/251‑1 (MQ=255)                                                                                                                                                                                                                          ttCAATTAAATAAAATCCAGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGAGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTgac                         >  2:87021/1‑248 (MQ=255)                                                                                                                                                                                                                                     aaaaTCCCGACTATTTCTTTATACGTTATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAgg             <  2:638908/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaaaTCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAg              <  2:477593/250‑1 (MQ=255)                                                                                                                                                                                                                                        aTCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGggt          >  1:194917/1‑251 (MQ=255)                                                                                                                                                                                                                                              aCTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGCGTATATCTCACTAACCCCTGACTAAGCAGATCTGAAT‑GGGATAAAGGGATGTAGCCATTTTTATATGGATTTCTTATGACAAAAATAATCGTCGATTTCCCCATAAAATATGAGCGATGCCAAAAAAAATCAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATg    >  1:284300/1‑251 (MQ=255)                                                                                                                                                                                                                                                tATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCTTAAGTGTATATCTCAATAACCCCTGCATAAGTAGCTCTGAATAGGTATAA‑TGGTTGAAGCCATTTTTAAATAGTTTTCTTATGCCAAAAATGGTCGTCGATTTCCCCATAAAATATGAGCGATGCCGAAAGAAGTAAAATTATTTATTACATTTAACGTTTGGATGAAAATTTAACATTTGTCATACAAATGGGGGGGGGTATGtt  >  2:410876/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        TTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAA‑GGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTT  >  NC_000913/321017‑321506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑