BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	99.2%	350.5 / NA	120	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (55/64); total (55/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 120 total aligned reads displayed.

CGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAC  >  NC_000913/284289‑284780

cggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCgg                                                                                                                                                                                                                                                      >  1:200982/1‑251 (MQ=255)

   cgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGCG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCACCATCGCGCCggc                                                                                                                                                                                                                                                     >  2:321644/1‑248 (MQ=255)

     ggCCGGAGGCGATGCGCCACCTGCGCGCCCTCGGCCTGCTCAATCTGGACGCCATGACCGTGACCGGCCAGACGGGGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGt                                                                                                                                                                                                                                                 <  2:239019/250‑1 (MQ=255)

      gCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGCCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGtt                                                                                                                                                                                                                                                >  1:104912/1‑251 (MQ=255)

        cGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCg                                                                                                                                                                                                                                              >  1:67416/1‑251 (MQ=255)

         ggAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCgg                                                                                                                                                                                                                                             >  1:494627/1‑251 (MQ=255)

          gatgtgctgcgcccccTGCGCCACCGCTGCCTGCTGCATCTGGACGCCATGACCGTGAACGGTCGGACGGTGGGCGAGATCCGTGAATGGTG‑GCTGTCGTCCGCGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGCCGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAATGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGt                                                                                                                                                                                                                                            <  2:497050/237‑1 (MQ=255)

          gAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGCGCCGGATGACGTGATCCTGCCGCCGGGGAAGGCAA‑CAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTGCGGt                                                                                                                                                                                                                                            >  2:185741/1‑251 (MQ=255)

          gAGGTGATGCTCCACCTGCCCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACGGGCCATGCGGTGGGCGAGAACTTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCTCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGt                                                                                                                                                                                                                                            <  1:389656/251‑1 (MQ=255)

              tGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTAGACGGTCGGCTGCCCGACGGGCAACATCGCGCCGGAAgggtgggggctc                                                                                                                                                                                                                                        >  2:487371/1‑241 (MQ=255)

              tGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTACGCCAGTGCCTGCGCGAGCAGGACGGCGTCGAGCCGGATGCCGTGCTCCTGCCGCCGGGGAAGGCCA‑AAGCGAAAGGGCTGGCCTCGACGGTCTGCCTCCCGACGGGCCACAGCGCGCCGGAAGGTTCggagagc                                                                                                                                                                                                                                        >  2:244894/1‑246 (MQ=255)

                aTGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCaa                                                                                                                                                                                                                                      <  1:219696/251‑1 (MQ=255)

                      cccccgcgcGACCTCGGCCTTCCGCATCGGGCCTCCAGGACCGCGACCGGCCAGACGGTGGGCGAGAACCGTGAAGTGTG‑GCAGGCGTCCGCGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGTCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCAc                                                                                                                                                                                                                                <  2:147281/246‑1 (MQ=255)

                      cACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGAGGTCAAGGCCAc                                                                                                                                                                                                                                >  2:60663/1‑251 (MQ=255)

                      cACCTGCGCGACCTCGACCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCCGGCCGGCGTAGCGCGGGATGACGTGATCCTGCCGCCGGAGAAGGCAACAAG‑GAAAGGGCGTACCTCGACGGTCTGCCTCCCGACGGGCAACATCGCGCCGGAAGGTTCggggcaccagtcccc                                                                                                                                                                                                                                >  2:114988/1‑238 (MQ=255)

                          ggggctcccgcggctggatgcATCTGGGCGCCTGGGCCGTGCCCGGCGTGGCGGGGGGCGAGAACTTAGAATGGTG‑GCAGGCGCCCGAGCGCGGGGCGCGTTGCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCGTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCg                                                                                                                                                                                                                            <  2:317431/233‑1 (MQ=255)

                           gcgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGc                                                                                                                                                                                                                             <  1:581099/249‑1 (MQ=255)

                                cccgcggccggcTGCATCGGGACGCCATGCCCGGGCCCGGCCAGACGGTGGGCGAGAACCTTGAATGGGG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAc                                                                                                                                                                                                                      <  2:332881/247‑1 (MQ=255)

                                 ccTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGCGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑CAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCgccc                                                                                                                                                                                                                     >  1:321195/1‑248 (MQ=255)

                                      gccggctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCg                                                                                                                                                                                                                <  1:121474/247‑1 (MQ=255)

                                       ccTGCTGCATCTGGACGCCAGGCCCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCgg                                                                                                                                                                                                               <  1:620730/251‑1 (MQ=255)

                                         gcgtgcctCTGCACGCCATACCCGTGCCCGGCCTGCCGGTGGGCTATATCCTTGAGTGGTG‑GCCGGCGTCCGAGCGCCGGGCGCGTTTCCGCCAGTGCCTGCGCGAGCAGGACGTCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAT‑AAGCGAAAGGGCTGACCTCGACGGTCTGGTTCCCGACGGGCAACATCGCGTCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtg                                                                                                                                                                                                             <  2:501373/244‑1 (MQ=255)

                                          gctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtgg                                                                                                                                                                                                            <  1:321644/251‑1 (MQ=255)

                                              catctgggcgctgtgcccgtgaccgGCCAGCGGGTGGGTGAGAACCTTGAAGGGTG‑GCAGGCGTCAGAGCGCCGGGCGCGCTGCCGCCAGTGCCTGCGCGAGCAGGACGGGGTAGAGCCGTATGATGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCGTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGACCACGGCGATCGTCCCGTCGGTGGTggg                                                                                                                                                                                                        <  2:455642/235‑1 (MQ=255)

                                               aTCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATACTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGc                                                                                                                                                                                                       >  1:424192/1‑251 (MQ=255)

                                                       gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCACCATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGAGGGCGAAGATgg                                                                                                                                                                                               >  1:637621/1‑251 (MQ=255)

                                                        ccATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGc                                                                                                                                                                                              >  1:636118/1‑251 (MQ=255)

                                                        ccATGACCGTGACCGGCCAGACGGTGGGAGAGAACCTTGACTGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAAGTGATCCTGCAGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGACCACATCGCGCCGGACGGGTCGGTGATCAGGGCCACGGCGATCGCCCCGGCGGTGGTGGGCGAAGGTggg                                                                                                                                                                                              >  2:67360/1‑250 (MQ=255)

                                                              ccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGCGCAGGACGGCGTAGAGCAGGCTGACGTGATCCTGCCGCCGGAGAAGGCAA‑CAGCGAAAGGGCTGCCCTCGACGGTCTGCTTCCCGGCGCGCACCATCGCGCCGGAAGGATCGGTGATCAAGGCCCCGGCGATCGACCCGTCGGTGGGGGGCGAAGAGGGCggacac                                                                                                                                                                                        >  2:530419/1‑246 (MQ=255)

                                                                  gaccgGCCAGCCGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATaccacc                                                                                                                                                                                    <  1:423209/251‑1 (MQ=255)

                                                                      ggccggccgtcgggCGAGACCCTTGAA‑GGTGTGCAGGCGTCCGAGCTCTGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGTACGGCGTAGAGCCTGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGTCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGCCCCGTCGGTGGTGTGCGAAGATGGCGTATACCTCCac‑ac                                                                                                                                                                               <  2:231073/240‑1 (MQ=255)

                                                                               gTGGGCGATAACCTGGAAGGGGG‑GCGGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGGGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGTCCCGTCGGTGGTGGGCGAAGATGGCGGATACCGCCAC‑ACCTGCcgggt                                                                                                                                                                      <  2:42603/251‑1 (MQ=255)

                                                                               gTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCcgggg                                                                                                                                                                      >  1:336377/1‑250 (MQ=255)

                                                                                gggggtagaacttgaagggggg‑gCAGGTGCCCGAGCGCGGGGGCCGCTCCCGCCAGTGCCTGCGAGAGCAGGACGGCGGAGAGCCGGATGACGTGAGCCTGCCGCCGGAGAAGGCAA‑GAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCcgggtg                                                                                                                                                                     <  2:454756/230‑1 (MQ=255)

                                                                                ggggCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCcgggtg                                                                                                                                                                     <  1:489801/250‑1 (MQ=255)

                                                                                     gagaACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCcgggtgcgggt                                                                                                                                                                >  1:110266/1‑251 (MQ=255)

                                                                                      agaACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCcgggtgcgggtg                                                                                                                                                               <  1:80132/251‑1 (MQ=255)

                                                                                       gaACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGgtgt                                                                                                                                                              <  1:625946/251‑1 (MQ=255)

                                                                                          ccTTGAATGGAG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGCTCCTGCCGCCGGAGAAAGCAA‑AAGCGAAAGGGCTGACCTCGAAGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAGGGTTCGGTGATCAAGGCCAAGGCGATCGACCCGTCGGTGGTGGGCGGAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTg                                                                                                                                                           >  2:432013/1‑251 (MQ=255)

                                                                                              gAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGtctc                                                                                                                                                       <  1:445943/251‑1 (MQ=255)

                                                                                                    tg‑gCAGGCGTACGAGCGCAGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGCTGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCAACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTTTACAACCAC‑ACCGGCCGGGTGAGGGTGTTTGTCTCGGGAgc                                                                                                                                                 >  2:433911/1‑251 (MQ=255)

                                                                                                       gCAGGCGCCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                                                                                               <  1:208753/251‑1 (MQ=255)

                                                                                                         gggCGCCCGGGCGCCGGGGGCGTTTCCGCCAGTGCATGCGCGAGCAGGACGGCGGAGAGCCGGAGGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGGCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcggag                                                                                                                                             <  2:258078/250‑4 (MQ=255)

                                                                                                         acgCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGCCGGGCAACATCGCGCCGGAAGGTTCGGTGCTCAAGGCCACGACGATCGACCCCTCGGTGGTGGGCGAAGATGGCGTCTACC‑CCACCACCGGCCGGGGGCGGGGGTTTGTCTCGGGAgcgc                                                                                                                                               >  2:6670/3‑249 (MQ=255)

                                                                                                             gTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGGGCGGGGGTTTGTCTCGGAAGCGCaggcgg                                                                                                                                         >  2:225271/1‑250 (MQ=255)

                                                                                                               ccGAGCGCCGGGAGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGCTCCTGCCGCCGGAGAAGGCAC‑AAGCGAAAGGGCTGACCTCGGCGGTCTGCTTCCCGACTGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAGGGCGTGTACCACCAC‑ACCGGCCGGCTGCGGGAGTTTGTCTCGGGAACGCaggcgatc                                                                                                                                       >  2:248387/1‑251 (MQ=255)

                                                                                                                    cgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggc                                                                                                                                  <  1:241346/251‑1 (MQ=255)

                                                                                                                     gtgtgggcgcgtcccgccatggCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGGGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCGCGGCGATCCACCCGTCGGTGGTGGGCGAAGATGGCGTATTCCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcg                                                                                                                                 <  2:407133/230‑1 (MQ=255)

                                                                                                                           cgcgcTCCCGCCAGTGCCTGCGCGGGCAGGACGGCGTAGAGCCGGATGTCGTGACCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCGTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCTTCGGTGGTGGGCGAAGATGGCGTAGACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGgcgatcaaggcgatcaag                                                                                                                           <  1:407217/251‑1 (MQ=255)

                                                                                                                                  ccGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGTCGATCAAGCGGGAag                                                                                                                    <  1:529698/251‑1 (MQ=255)

                                                                                                                                    gCCAGTGCCTTCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAagag                                                                                                                  <  1:659297/251‑1 (MQ=255)

                                                                                                                                    gCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGACACGCAGGCGATACAGGCGATCAAGCGGGAag                                                                                                                    >  2:169323/1‑249 (MQ=255)

                                                                                                                                     ccAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGGGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGACGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCggggagggg                                                                                                                 >  2:289486/1‑245 (MQ=255)

                                                                                                                                      cAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAag                                                                                                                    >  1:42043/1‑247 (MQ=255)

                                                                                                                                           ccagcgcGAGCAGGACGGCGTAGAGCCGGATGCAGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCGTACCTCGACGGAATGCTTCCCGACAGGCAACATCGCGCCGGAAGGTTCGGTGATGAAGGACACGGCGATCGGACCTTCGGTGGTGGGCGAAGAAGGGGTATACCCCCCC‑ACCGACCGGGGGCGGGAGATTGCCTAGGAAGCGGAGGCGATCAAGGCGATCACGCGGGAGGAGA‑Ttgggc                                                                                                          >  2:58685/4‑248 (MQ=255)

                                                                                                                                              gcgcGAGAAGGACGGCGTAGAGCCGGATGACGGGATCCGTCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGGCTGCTGCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGTCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAg                                                                                                        <  2:610586/250‑1 (MQ=255)

                                                                                                                                                   aGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGat                                                                                                  >  1:459049/1‑251 (MQ=255)

                                                                                                                                                     cAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGatat                                                                                                >  1:677080/1‑251 (MQ=255)

                                                                                                                                                       ggACGGCGTAGAGCCGGAAGCCGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCa                                                                                              <  2:40621/251‑1 (MQ=255)

                                                                                                                                                          cGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCAtgg                                                                                           >  1:625542/1‑251 (MQ=255)

                                                                                                                                                              gTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATggtggt                                                                                       >  1:153294/1‑251 (MQ=255)

                                                                                                                                                              gTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATggtggt                                                                                       >  1:439187/1‑251 (MQ=255)

                                                                                                                                                                     cGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGGAGAGACTTG‑GCAGGGCGAAATCAtggtggggagcggg                                                                                >  2:345960/1‑243 (MQ=255)

                                                                                                                                                                      ggATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATcggcg                                                                               >  1:127955/1‑251 (MQ=255)

                                                                                                                                                                      ggATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATcggcg                                                                               <  1:555566/251‑1 (MQ=255)

                                                                                                                                                                      ggATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACAGGCCGGGTGCGGGTGGTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCGTGGTGGTGATcggcg                                                                               >  1:76220/1‑251 (MQ=255)

                                                                                                                                                                       gATGACGTGATCCTGCCGCCGGAGAAGGAAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑CCCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGCAGGCGCTCAAGGCGCTCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATcggcgg                                                                              >  1:658784/1‑251 (MQ=255)

                                                                                                                                                                        gtggcgggATCCGGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATcggcggc                                                                             <  2:269329/244‑1 (MQ=255)

                                                                                                                                                                        aTGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATcggcggc                                                                             <  1:299743/251‑1 (MQ=255)

                                                                                                                                                                                aTCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAgaga                                                                                                                 <  1:297044/208‑1 (MQ=255)

                                                                                                                                                                                aTCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTc                                                                     >  1:660915/1‑251 (MQ=255)

                                                                                                                                                                                 tCCTGCCGCCGGAGAAGGCAA‑AAGCGAACGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                    >  1:242055/1‑251 (MQ=255)

                                                                                                                                                                                 cccTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                    <  1:23274/250‑1 (MQ=255)

                                                                                                                                                                                 cccTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCGGCCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                    <  2:333296/250‑1 (MQ=255)

                                                                                                                                                                                  cccgccgccGGAGAAGGCAA‑AAGCGAAAGGGCTCGCCTCGACGGGCTGCTGCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                    <  2:282119/247‑1 (MQ=255)

                                                                                                                                                                                  ccTGTCGCCGGAGAAGTCAA‑AAGCGAAAGGGCTGACCCCGACGGTCTGCTGCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGCACCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCggcc                                                                    <  2:330867/250‑4 (MQ=255)

                                                                                                                                                                                  ccTGCCGCCGGAGAAGGCAA‑AAGCGAATGGGCGGACCGCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccg                                                                   <  2:459049/251‑1 (MQ=255)

                                                                                                                                                                                     gccgccGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccgg                                                                  <  1:6670/249‑1 (MQ=255)

                                                                                                                                                                                          cgGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCCC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGTTCAAGCGGGAAGAGA‑TTGTGGAGGGCGATTACATGGCGGTGATCGGGTGGGGGGCGGccgg‑caccggg                                                          >  2:311572/1‑250 (MQ=255)

                                                                                                                                                                                           ggAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccgg‑caccggc                                                          >  1:222869/1‑250 (MQ=255)

                                                                                                                                                                                                aGGCAA‑AAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGACGGGCCACATCGCGCCGGAAGGTTCGGTGAGCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCCCCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGAGCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGGCCGG‑CACCGGCATGGaa                                                    <  2:287670/251‑1 (MQ=255)

                                                                                                                                                                                                 ggCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAgg                                                                                                       >  1:535651/1‑200 (MQ=255)

                                                                                                                                                                                                 ggCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAgg                                                                                                       <  2:535651/200‑1 (MQ=255)

                                                                                                                                                                                                  gCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGa                                                     >  1:451587/1‑248 (MQ=255)

                                                                                                                                                                                                  gCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAaga                                                  <  1:487371/251‑1 (MQ=255)

                                                                                                                                                                                                    aa‑aaGCGAAAGGGCTGCCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAag                                                   >  1:586440/1‑248 (MQ=255)

                                                                                                                                                                                                     a‑aaGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGAc                                               <  1:88341/251‑1 (MQ=255)

                                                                                                                                                                                                         gCGAAAGGGCTGACCGCTACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCGCGGCGATCGACCAGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGAGCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGAGATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTa                                            <  2:110266/251‑1 (MQ=255)

                                                                                                                                                                                                           gaaggggCTGTCCTCGGCTGTCTGCTTGCCGACGGGCAACCTCGCGCCGGAAGGTTCGGGGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAGGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGGGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTAca                                          <  2:453949/247‑2 (MQ=255)

                                                                                                                                                                                                            aaaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGCGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCACGCGGGCAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGGCGTCCGG‑CACCGGCGTGGAAGAGACCtgac                                          >  2:136081/1‑247 (MQ=255)

                                                                                                                                                                                                             aaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAg                                        <  1:114988/251‑1 (MQ=255)

                                                                                                                                                                                                              agtgCTGCCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCTACCCGTCGTTGGTGGGTGAAGATGGCGTATACCACCAC‑TCCGGCCTGTTGCGGGTGTTTGGCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGAGTGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGc                                       <  2:424192/248‑1 (MQ=255)

                                                                                                                                                                                                                    ggccTCGACGTTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCAc                                   <  1:440175/247‑1 (MQ=255)

                                                                                                                                                                                                                    ggccTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAATATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCAc                                   <  2:222869/247‑1 (MQ=255)

                                                                                                                                                                                                                     aCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCTc                                >  1:241248/1‑251 (MQ=255)

                                                                                                                                                                                                                           aCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGGTCAAGCGGGAAGCGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGGCAC‑GGCATGGGAGAGGCCTACCCGCCCACCTCcgcccc                          >  2:150965/1‑248 (MQ=255)

                                                                                                                                                                                                                                  gCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCat                   <  1:238586/251‑1 (MQ=255)

                                                                                                                                                                                                                                           cGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATAGCTCGTgg          <  1:58685/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  cATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGcc      >  1:559583/1‑247 (MQ=255)

                                                                                                                                                                                                                                                   aTCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCGCCGCGCTAAAGCATATCTCGTGGGGCAAGAc  <  1:662136/251‑1 (MQ=255)

CGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTG‑GCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAA‑AAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCAC‑ACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGA‑TTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGG‑CACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAC  >  NC_000913/284289‑284780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`