Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,901	G→A	pseudogene (138/427 nt)	insN →	IS911A regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,901	0	G	A	93.2%	100.1 / 1.2	45	pseudogene (138/427 nt)	insN	IS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (20/21);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01

ATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTC  >  NC_000913/271657‑272103                                                                                                                                                                                                                                                     |                                                                                                                                                                                                           caaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcagac                                                                                                                                                                                                      <  1:2382‑M1/146‑1 (MQ=255)    aacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAACCcagacggc                                                                                                                                                                                                   <  2:160079‑M1/149‑1 (MQ=255)        ccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcagacggcagac                                                                                                                                                                                               >  2:340009‑M1/99‑251 (MQ=255)         caaaagcctctcctataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcagacggcagacg                                                                                                                                                                                              >  2:2382‑M1/98‑251 (MQ=255)          aaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcagacggcagacgg                                                                                                                                                                                             >  1:334266‑M1/97‑251 (MQ=255)             agcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCt                                                                                                                                                                                          >  1:208152‑M1/94‑251 (MQ=255)                  ctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTAtt                                                                                                                                                                                     <  1:411672‑M1/163‑1 (MQ=255)                    ccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTAtt                                                                                                                                                                                     >  1:260615‑M1/87‑249 (MQ=255)                       ataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattacaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGc                                                                                                                                                                                 <  1:227835‑M1/167‑1 (MQ=255)                       ataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCtgtattccgc                                                                                                                                                                                 <  1:628708‑M1/167‑5 (MQ=255)                              cagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGt                                                                                                                                                                         >  1:272061‑M1/77‑251 (MQ=255)                                         gaaataaataagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGACACACTCTGCCATGTGTTCGGGGTTCATCACCGCAGCTACAGATACTGGAAAACCCATCCTGAAAAACCAGACGGCAGACGGGCTGTattacgcagtcagtgaattgaactc                                                                                                                                                               <  2:631024‑M1/185‑13 (MQ=255)                                           aatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGgtacttgagctacat                                                                                                                                                            >  2:382304‑M1/64‑244 (MQ=255)                                                  cAAAACACCGAAAGAGATAATGGAAAGGGGTGTTGCAGTGACAGAGTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCGCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTGCGGGGTTCATCGCAGCAGCTACAAACACTGGAAAGACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATc                                                                                                                                                      <  1:292170/250‑1 (MQ=39)                                                  aagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATc                                                                                                                                                      <  1:5366‑M1/194‑1 (MQ=255)                                                  aagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCa                                                                                                                                                     >  1:160079‑M1/57‑251 (MQ=255)                                                             aaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGccacggttcg                                                                                                                                          <  1:198662‑M1/206‑5 (MQ=255)                                                               agctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGACATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGAAGACGGGCTGTATTACGCAGTCCGGTACTTGAGCTACatggcctcagccccggttcggc                                                                                                                                        <  2:495355‑M1/208‑11 (MQ=255)                                                                    caacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAg                                                                                                                                   >  1:224230‑M1/39‑251 (MQ=255)                                                                      acgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGc                                                                                                                                  <  1:286888‑M1/214‑1 (MQ=255)                                                                               aatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACggg                                                                                                                                                                                            >  1:233165‑M1/28‑183 (MQ=255)                                                                                  ggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGcc                                                                                                                     <  1:147007‑M1/227‑1 (MQ=255)                                                                                     gaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGAAACTGGAAAAACCGTCCTGAAAAACCCGACGGCAGACGGGCTGAATTACGCAGTAAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATcgccgc                                                                                                                   <  2:573284‑M1/229‑3 (MQ=255)                                                                                        tgaaatattaaaaaggctACAGAGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATg                                                                                                               <  2:494678‑M1/233‑1 (MQ=255)                                                                                          aaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATCATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTCTTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGc                                                                                                             <  2:336895‑M1/235‑1 (MQ=255)                                                                                                taaaaatgctTCCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCGCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGTTCACACTCTGCCATTTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTGCGGCCGGAGCAATAAGCATCGCCACAATGGCAAccc                                                                                                        >  2:268440‑M1/11‑250 (MQ=255)                                                                                                  aaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCaacccgg                                                                                                      <  1:540371‑M1/242‑3 (MQ=255)                                                                                                     aggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCggagag                                                                                                  >  1:336895‑M1/6‑251 (MQ=255)                                                                                                      ggctaCCGCGCTCTTGATGTCCGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCATAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTTGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGAACTTGCGCTACATAGCATCAGACACGGTTCGGCCGGAGCAAGACGCATCGCCACAATGGCCACCCggaga                                                                                                   >  2:643164/5‑249 (MQ=18)                                                                                                      ggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTTTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCggaga                                                                                                   >  1:643164‑M1/5‑249 (MQ=255)                                                                                                          tccGCGCTCTTGATGTCAGTCTCCCTGAACAGTTCACGATAATCGGGAAACGCAGATCGCATTTACCTTTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAggcta                                                                                              <  2:552735/249‑4 (MQ=17)                                                                                                                    tGATGTCAGACTCCCGGAACAGTTCTCGAGAAGCGGGAAACTCAGAGCGCATTATCCTGTGGTCCCACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTTAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCTGGAGAGGCTACCAGATggga                                                                                    <  2:568070/250‑2 (MQ=17)                                                                                                                     gctGTCAGACTCCCTGAACAGTGCTCGATAAACGGGAAACTCTGAGCGCCTTCTACTGTGGTCACTCTCTGGCAAGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTTGAAAAACCGTCCTGAAAAACCAGACGGCTGACGGGCTGAATTACGCAGTCAGGTACTTGAGCTACATCGCATCAGCACCGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATgggat                                                                                   <  2:182460/248‑3 (MQ=9)                                                                                                                                 acgctctgccgaactaTAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCt                                                                        <  2:255338/234‑1 (MQ=14)                                                                                                                                        aGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTc                                                               >  1:370654/1‑251 (MQ=14)                                                                                                                                         gTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTc                                                               >  1:167215/1‑250 (MQ=14)                                                                                                                                              cGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGaa                                                          >  1:442682/1‑250 (MQ=14)                                                                                                                                                aTAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCt                                                                        >  1:255338/1‑234 (MQ=14)                                                                                                                                                  aaTCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAagag                                                      <  2:411672/250‑1 (MQ=14)                                                                                                                                                     cGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTgg                                                  >  1:649254/1‑251 (MQ=14)                                                                                                                                                       ggAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTg                                                                               <  1:271801/220‑1 (MQ=14)                                                                                                                                                       ggAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTgggg                                                >  1:389421/1‑251 (MQ=11)                                                                                                                                                          aaCTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGGTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTGCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACAAGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTg                                             <  1:4290/251‑1 (MQ=11)                                                                                                                                                                             tgtgGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCg                           >  1:323451/1‑250 (MQ=255)                                                                                                                                                                                                      ggggTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCgggc  >  1:8023/1‑248 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                           ATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTCACCGGTATAAGCGTGGCGGTC  >  NC_000913/271657‑272103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑