| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 154,182 | C→T | L415L (CTG→CTA) | htrE ← | putative fimbrial usher protein HtrE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 154,182 | 0 | C | T | 91.4% | 91.1 / ‑0.3 | 36 | L415L (CTG→CTA) | htrE | putative fimbrial usher protein HtrE |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (19/13); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
ATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAT > NC_000913/153944‑154427 | aTTTCGGTGCAAGATCTTGTGCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAATGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTTCTTGTTTCGTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTAAGTTTTATCAGCCGGGATACGAACATTGTAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCt < 1:614776/251‑1 (MQ=255) ttGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGtat > 1:328318/1‑251 (MQ=255) tCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGt < 1:614461/250‑1 (MQ=255) cGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGtta < 1:539721/251‑1 (MQ=255) aTGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTtattat < 1:203602/251‑1 (MQ=255) catcaATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTg < 2:250301/250‑1 (MQ=255) gagTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGttatt > 1:240921/1‑229 (MQ=255) gagTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGttatt < 2:240921/229‑1 (MQ=255) gTGCATCATTAAGACCAAGGTAATTCTGTGGCGAATAGAGATAGGCCGCGATATTCAGAGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCAGGGATAAGAACATTGGAATGAGTAACA‑TCGAACGAAAATGCACCAACTGAAGTATTTAGAACAAGACCTAACAAACCAACGGTATAGTTTTTATCGGTAATCTGAATACCGGaataccac > 2:74334/1‑247 (MQ=255) cAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTc > 1:626850/1‑251 (MQ=255) aaGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATc < 2:551518/217‑1 (MQ=255) tCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgt > 1:588419/1‑249 (MQ=255) tgtCGAATAGCGATAGGACGCGATATTCAGTGAAGCACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGCCCTAACAAAACAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtagtagt > 2:613020/1‑251 (MQ=255) gtCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtagtagt > 1:546221/1‑250 (MQ=255) atcgCGAAAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTAAGACCAAGACCTAACAAACCAGCGGTATAGTTATTCTCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTAttaaagttcggcaaagcgtggt > 2:41729/4‑231 (MQ=255) aTAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCa < 1:41729/233‑1 (MQ=255) caggtaaGTCCTTGTTTCTTCGAATAAATTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTTACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATATTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCa < 2:30606/245‑1 (MQ=255) aGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGAATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATc > 1:160829/1‑251 (MQ=255) gTGAAGTCCTTGTTTCTTCGAATAACTTGTTACAGGAAACACGATAACTTTGCCAATGGTATGTTTTATCATCCGGGATACGAACATTGGATTGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGAACAAGACCTAACAAACCAGCGGTATCGATATTATCGGTTATCTGACTACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGGAGTAGCTTGCTAGAAATAAATTAGTCTCATcc > 2:624676/1‑251 (MQ=255) aCTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAatat > 1:313567/1‑251 (MQ=255) cTTGTTTCTTCGAATATCTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAAccc < 2:190662/186‑1 (MQ=255) cTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAAccc > 1:190662/1‑186 (MQ=255) tcGAATAACTTGTTACAGGAAACACGATAACATTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCtttt < 1:312149/249‑1 (MQ=255) aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGAcc < 1:602201/251‑1 (MQ=255) ttGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGCCCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATACTGAATATCATCTTTTAAGACCTGAcc > 1:422626/1‑251 (MQ=255) gAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAat > 1:438261/1‑251 (MQ=255) aaCTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGGAGTAGCTTGCTTGAAATAAAATAAGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGt > 2:434260/1‑251 (MQ=255) ttttATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGTAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAACTTGCTTGAAATAAATTAGGCTCATCCTGAATCTCATCTTTTAAGCCCTAACCACCGCTAATATCCCAACGTCCAACGCCATGTCGTAAc > 2:183556/1‑251 (MQ=255) cGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTCACATTTGcaca > 1:517318/1‑250 (MQ=255) ggAATGAGT‑ACAATCGAAAGAAAATGCACCAACTGAAGTTTTTAGACCAAGACCTAAAAAACCAGCGGTATATTTATTATGGGTTATCTGAATCCCGTTATAACCCGTCAGATTGTTATTCAGGCCGTAGTAGTAGCTTGCTTAAAATAAATTAGGCTCATCCTGAATTTCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGt < 1:502767/250‑1 (MQ=255) a‑TCGAAAAAAAATGCACAAACTGAAGTATTTAGACCAAGACCTAAAAAACCAGCGGTATAGTTATTACCGTTTATCTGAACACCGGTATAACCCGTCAGATTGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTTAATATCATCTTTAAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTcc < 1:613020/251‑1 (MQ=255) aagaaaATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCttt > 1:157923/1‑251 (MQ=255) aaTGCACCACCTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGTTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAACTAAATTAGGCTCATCCTGAATATCCTCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGGCCGCTTTGAGcc > 2:662467/1‑251 (MQ=255) aaCTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGAtt > 1:201892/1‑251 (MQ=255) gAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGAATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGGTGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGAttcttc > 2:7360/1‑251 (MQ=255) tATTTAGACCAAGACCTAAAAAACCAGCGGTATAGTTATTATCGGTTATCTAAATACCGGTATAACCCGTCAGATATTTATTCAGGCCGTATTAGTAGCTTGCTGGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATATCCGCTTTGAGCCATCGGATTCTTCGAt < 1:437488/250‑1 (MQ=255) | ATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAT > NC_000913/153944‑154427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |