Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913154,1820CT91.4% 91.1 / ‑0.3 36L415L (CTG→CTAhtrEputative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (19/13);  total (21/15)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAT  >  NC_000913/153944‑154427
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     
aTTTCGGTGCAAGATCTTGTGCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAATGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTTCTTGTTTCGTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTAAGTTTTATCAGCCGGGATACGAACATTGTAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCt                                                                                                                                                                                                                                           <  1:614776/251‑1 (MQ=255)
                ttGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGtat                                                                                                                                                                                                                           >  1:328318/1‑251 (MQ=255)
                    tCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGt                                                                                                                                                                                                                        <  1:614461/250‑1 (MQ=255)
                     cGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGtta                                                                                                                                                                                                                      <  1:539721/251‑1 (MQ=255)
                         aTGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTtattat                                                                                                                                                                                                                  <  1:203602/251‑1 (MQ=255)
                                    catcaATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTg                                                                                                                                                                                                        <  2:250301/250‑1 (MQ=255)
                                             gagTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGttatt                                                                                                                                                                                                                    >  1:240921/1‑229 (MQ=255)
                                             gagTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGttatt                                                                                                                                                                                                                    <  2:240921/229‑1 (MQ=255)
                                                   gTGCATCATTAAGACCAAGGTAATTCTGTGGCGAATAGAGATAGGCCGCGATATTCAGAGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCAGGGATAAGAACATTGGAATGAGTAACATCGAACGAAAATGCACCAACTGAAGTATTTAGAACAAGACCTAACAAACCAACGGTATAGTTTTTATCGGTAATCTGAATACCGGaataccac                                                                                                                                                                                        >  2:74334/1‑247 (MQ=255)
                                                                  cAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTc                                                                                                                                                                         >  1:626850/1‑251 (MQ=255)
                                                                   aaGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATc                                                                                                                                                                                                          <  2:551518/217‑1 (MQ=255)
                                                                           tCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgt                                                                                                                                                                  >  1:588419/1‑249 (MQ=255)
                                                                               tgtCGAATAGCGATAGGACGCGATATTCAGTGAAGCACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGCCCTAACAAAACAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtagtagt                                                                                                                                                            >  2:613020/1‑251 (MQ=255)
                                                                                gtCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtagtagt                                                                                                                                                            >  1:546221/1‑250 (MQ=255)
                                                                                     atcgCGAAAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTAAGACCAAGACCTAACAAACCAGCGGTATAGTTATTCTCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTAttaaagttcggcaaagcgtggt                                                                                                                                                      >  2:41729/4‑231 (MQ=255)
                                                                                     aTAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCa                                                                                                                                                                        <  1:41729/233‑1 (MQ=255)
                                                                                                          caggtaaGTCCTTGTTTCTTCGAATAAATTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTTACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATATTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCa                                                                                                                                  <  2:30606/245‑1 (MQ=255)
                                                                                                           aGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGAATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATc                                                                                                                                >  1:160829/1‑251 (MQ=255)
                                                                                                            gTGAAGTCCTTGTTTCTTCGAATAACTTGTTACAGGAAACACGATAACTTTGCCAATGGTATGTTTTATCATCCGGGATACGAACATTGGATTGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGAACAAGACCTAACAAACCAGCGGTATCGATATTATCGGTTATCTGACTACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGGAGTAGCTTGCTAGAAATAAATTAGTCTCATcc                                                                                                                               >  2:624676/1‑251 (MQ=255)
                                                                                                                   aCTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAatat                                                                                                                        >  1:313567/1‑251 (MQ=255)
                                                                                                                    cTTGTTTCTTCGAATATCTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAAccc                                                                                                                                                                                        <  2:190662/186‑1 (MQ=255)
                                                                                                                    cTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAAccc                                                                                                                                                                                        >  1:190662/1‑186 (MQ=255)
                                                                                                                             tcGAATAACTTGTTACAGGAAACACGATAACATTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCtttt                                                                                                                <  1:312149/249‑1 (MQ=255)
                                                                                                                                 aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGAcc                                                                                                          <  1:602201/251‑1 (MQ=255)
                                                                                                                                      ttGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGCCCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATACTGAATATCATCTTTTAAGACCTGAcc                                                                                                     >  1:422626/1‑251 (MQ=255)
                                                                                                                                               gAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAat                                                                                            >  1:438261/1‑251 (MQ=255)
                                                                                                                                                         aaCTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGGAGTAGCTTGCTTGAAATAAAATAAGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGt                                                                                  >  2:434260/1‑251 (MQ=255)
                                                                                                                                                                           ttttATCATCCGGGATACGAACATTGGAATGAGTCACATCGTAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAACTTGCTTGAAATAAATTAGGCTCATCCTGAATCTCATCTTTTAAGCCCTAACCACCGCTAATATCCCAACGTCCAACGCCATGTCGTAAc                                                                >  2:183556/1‑251 (MQ=255)
                                                                                                                                                                                     cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTCACATTTGcaca                                                       >  1:517318/1‑250 (MQ=255)
                                                                                                                                                                                                    ggAATGAGTACAATCGAAAGAAAATGCACCAACTGAAGTTTTTAGACCAAGACCTAAAAAACCAGCGGTATATTTATTATGGGTTATCTGAATCCCGTTATAACCCGTCAGATTGTTATTCAGGCCGTAGTAGTAGCTTGCTTAAAATAAATTAGGCTCATCCTGAATTTCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGt                                        <  1:502767/250‑1 (MQ=255)
                                                                                                                                                                                                                aTCGAAAAAAAATGCACAAACTGAAGTATTTAGACCAAGACCTAAAAAACCAGCGGTATAGTTATTACCGTTTATCTGAACACCGGTATAACCCGTCAGATTGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTTAATATCATCTTTAAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTcc                           <  1:613020/251‑1 (MQ=255)
                                                                                                                                                                                                                      aagaaaATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCttt                      >  1:157923/1‑251 (MQ=255)
                                                                                                                                                                                                                           aaTGCACCACCTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGTTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAACTAAATTAGGCTCATCCTGAATATCCTCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGGCCGCTTTGAGcc                 >  2:662467/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aaCTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGAtt         >  1:201892/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGAATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGGTGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGAttcttc     >  2:7360/1‑251 (MQ=255)
                                                                                                                                                                                                                                           tATTTAGACCAAGACCTAAAAAACCAGCGGTATAGTTATTATCGGTTATCTAAATACCGGTATAACCCGTCAGATATTTATTCAGGCCGTATTAGTAGCTTGCTGGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATATCCGCTTTGAGCCATCGGATTCTTCGAt  <  1:437488/250‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     
ATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAT  >  NC_000913/153944‑154427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: