Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,661	T→C	N285N (AAT→AAC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,661	0	T	C	100.0%	178.6 / NA	59	N285N (AAT→AAC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/28);  total (31/28)

GCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGAT  >  NC_000913/46418‑46904                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gctaGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTCCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGa                                                                                                                                                                                                                                                <  1:224068/248‑1 (MQ=255)                cGAAGAGATGATGATAAAACTGTTTGGCGAACCGTTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCACTGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCACAAACGCGGCGCTGGGGAACGTGATGATTAGCCTGTTCttt                                                                                                                                                                                                                                  >  1:196033/1‑250 (MQ=255)                cGAAAAGATGATGAAAAAACCGTTTTGCGAACCGGTGGCTTTCGATGAAAAGCGGCAGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCCTTTTCCTTTTGTTCTGTTTGTTGACGCCATCTGGACCTGCCATGTGATCCCAATGTTCTCCATTTACACCTTTGGCCCGCAAATCGTTAGTTTGTTGGGATTGAGTGTTGGCAAAAACGCGGCGCTGGGGTACGTGGTGAGTAGCCTGTTGttt                                                                                                                                                                                                                                  >  2:632653/1‑250 (MQ=255)                 gAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAATCGCGGCGCTGGGGCACGTGGTGATTAGCCTGTTCTTTAt                                                                                                                                                                                                                                >  2:341949/1‑251 (MQ=255)                       atgatgatAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCAATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGACTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCgg                                                                                                                                                                                                                          <  1:4070/251‑1 (MQ=255)                          atgatAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTg                                                                                                                                                                                                                       >  1:90872/1‑251 (MQ=255)                           tgataaaccTGTTTGGCGAACCGGTGGCTATCGATCAAGAGCAGCCTCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTGTCCTTTTGATCCGCTTGTTGCCGACATCTGGACCTGCCAGGCGATCCTACTGCTCGCCATTTACACCCTTGGACCGAAAATCGTTGGTTTGTTGGGATTGGGCATTAGCAGAAAAGCGGAGTTGGGGTACGTGGGGTTTAGCCTGTTATTTATGCTCGGCTGt                                                                                                                                                                                                                      >  2:515448/9‑251 (MQ=255)                                aaaCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTcc                                                                                                                                                                                                                 >  2:118849/1‑251 (MQ=255)                                 aaCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccg                                                                                                                                                                                                                <  1:329949/251‑1 (MQ=255)                                     gTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccgccg                                                                                                                                                                                                             <  1:218096/250‑1 (MQ=255)                                                    ggtcttctatgaatagacgcCGCAGCAAACCCTTTTGCGCGATCTCTTTATTCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCAGTTACACCTTTGGCCCGCAAATCGTTGGGTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTTGGGACCGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAaca                                                                                                                                                                                             <  2:622451/233‑1 (MQ=255)                                                                 gagCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCt                                                                                                                                                                                                                                         >  1:3976/1‑194 (MQ=255)                                                                     agcCGCCGCAAACCCGTTTTCGCGATCTGTTTAATAGCCGCCCTTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCCTTTCCACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGACCGTGGTGATTAg                                                                                                                                                                                                                                            >  2:402800/1‑187 (MQ=255)                                                                     agcCGCAGCAAACCCGTTTTTGCGATCTGTTTAATCGCCGCCAGTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCCGCCAGGTGATCCCAATGTTCGCCATTTACCCCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAg                                                                                                                                                                                                                                            <  1:402800/187‑1 (MQ=255)                                                                         gcagcaAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCAttgt                                                                                                                                                                        >  1:237571/1‑251 (MQ=255)                                                                               aaCCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTg                                                                                                                                                                  >  1:243067/1‑251 (MQ=255)                                                                               aaCCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGCGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAATAACTGCCGGACGGCGTCCATTGTTGATTg                                                                                                                                                                  >  1:635782/1‑251 (MQ=255)                                                                                      tttCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTTTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCAGTGTTGATTGGCAGCt                                                                                                                                                            <  1:580026/250‑1 (MQ=255)                                                                                                tGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGACATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGGCCATTGTTGATTGGCAGCTTTGCCatgatg                                                                                                                                                 >  2:584196/1‑251 (MQ=255)                                                                                                 gTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCat                                                                                                                                                     <  1:178797/246‑1 (MQ=255)                                                                                                     aaTCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGCCCTGCCAGGTGAGCCCAATGTTCGCCATTTACACCTTGGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgct                                                                                                                                            <  1:616268/251‑1 (MQ=255)                                                                                                        cgccgcCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAacac                                                                                                                                                                                            >  2:155807/1‑200 (MQ=255)                                                                                                        cgccgcCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAacac                                                                                                                                                                                            <  1:155807/200‑1 (MQ=255)                                                                                                        cgccgcCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggc                                                                                                                                         >  1:377468/1‑251 (MQ=255)                                                                                                            gccATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAACTCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGCCGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggctctg                                                                                                                                     >  2:565165/1‑251 (MQ=255)                                                                                                                 tttCCTTTTGTTCTGTTTGTTGCCGCCATCTGGGCCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAAGCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCgg                                                                                                                                 <  1:142829/250‑1 (MQ=255)                                                                                                                  ttCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCgggt                                                                                                                               >  1:460681/1‑249 (MQ=255)                                                                                                                     cTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTTCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGTCGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTg                                                                                                                            >  1:182377/1‑251 (MQ=255)                                                                                                                     cTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTGTTg                                                                                                                            >  1:244881/1‑251 (MQ=255)                                                                                                                          gTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCt                                                                                                                       >  1:209545/1‑251 (MQ=255)                                                                                                                               gtttgttGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATcc                                                                                                                  >  1:436444/1‑251 (MQ=255)                                                                                                                               gtttgtgGCCCCCATCTGGACCTGCCAGTGGATCCCAATGTTCGCCATTTACTCCTGTGGCCCGCAAATCGTTGTGTTGTTGGGATTGGTTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTGCTTTAGGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGTCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATcc                                                                                                                  <  2:237571/251‑1 (MQ=255)                                                                                                                                         gccATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGTTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCCAGATGACGCTGGCGATGACGGTTTTtggggtggttcacgtgatggg                                                                                                        >  2:367092/1‑234 (MQ=255)                                                                                                                                          ccATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGGTCCCGGGTATgggg                                                                                                       >  1:404272/1‑251 (MQ=255)                                                                                                                                              cTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCACATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTACACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTgggggttgtgccgggtttggggatct                                                                                                   >  2:555232/1‑229 (MQ=255)                                                                                                                                               tggcccTGCCAGGTGATCCCAATGTTTGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTGGGTATTGGTTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGAt                                                                                                                                                                                                           <  2:22434/142‑1 (MQ=255)                                                                                                                                               tGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGAt                                                                                                                                                                                                           >  1:22434/1‑146 (MQ=255)                                                                                                                                               gggACCTGCCAGGGGATCCCAAGGTTCGCCATTTACGCCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATctg                                                                                                  <  2:90185/250‑1 (MQ=255)                                                                                                                                                ggACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATct                                                                                                   <  1:118849/249‑1 (MQ=255)                                                                                                                                                 gACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGGATTCCGCCGATGCTGTGGTTCAACACTGCCGGAAGGCGTCCATTGTTGATTGGCAGCTTTGCCATGGTGAATCCGGAGCTGGCGGTTTTGGGGCtgggccacgacattgggggttggt                                                                                                >  2:22015/1‑228 (MQ=255)                                                                                                                                                   ccTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATctggctg                                                                                              <  1:493978/251‑1 (MQ=255)                                                                                                                                                          ggTGATCCGAATGTGCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATTCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGAGTGGCAGCTTTGCCATGATTACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGa                                                                                       <  1:646487/251‑1 (MQ=255)                                                                                                                                                                 ccAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCtt                                                                                <  1:112850/251‑1 (MQ=255)                                                                                                                                                                     tGTTCGCCATTTACCCCTTTGGCCCGCAAATCGTTGGTTTGGTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggc                                                                                                                                         <  2:557159/190‑1 (MQ=255)                                                                                                                                                                     tGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggc                                                                                                                                         >  1:557159/1‑190 (MQ=255)                                                                                                                                                                      gTTCTCCAGTTACCCCTTTGGCCCGCAAATCGGTGGTTTTGGGGGATTGGGGGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCCCGGCTGTATTCCGCCGATGCTGTGGTTAAACCCTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATTACGCGGGCGCTGGCTGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGTGCTTTGct                                                                            <  2:218386/250‑2 (MQ=255)                                                                                                                                                                            cATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTTCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATg                                                                    <  1:640553/251‑1 (MQ=255)                                                                                                                                                                             aTTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑c                                                                  <  1:555232/251‑1 (MQ=255)                                                                                                                                                                                 acacCTTTGGCCCGCAAATCGTTGGGTTGTTTGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCtt                                                               <  2:171072/250‑1 (MQ=255)                                                                                                                                                                                           cccGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGTCGCTGGGGAACGTGGTGATGAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGTTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTTTCTCTGGCg                                                    <  1:410519/251‑1 (MQ=255)                                                                                                                                                                                               aaaaTCGTGGGGTTGATGGGATGGGGTGTTGGCAAAAACGCGGCGCTGGGAAACGTGGTGATTAGCCTGTTCTTTATGCGCGGCTGTATGCCGCCGATGCTGTGGTTAAACACTGCCTGACGTCGTCCATTGTTGATTGGCAGCTGTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCGTATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTGTCTCTGGCGGGcc                                                <  2:644146/250‑1 (MQ=255)                                                                                                                                                                                                            tgttgGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTTTCTCTGGCGGGCCGGGTAATTTGCa                                    <  1:565165/250‑1 (MQ=255)                                                                                                                                                                                                             gttgGGATTGGGAGTTGGCAAAAACGCGGCGCTGGGGAACGCGGTGATTAGCCTGTTCTTTATGCTCGGATGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCTATGATGACGCTGGCGCTGGCGGCTTTGGGGCTGATACCGGGTATGGGGATCTCGCTGGGTGGGGTGGCCTTTGCGGAG‑TATGGCCTTTTT‑TCTCGCGGGACGGGGAATTTGCAgg                                  >  1:43415/1‑250 (MQ=255)                                                                                                                                                                                                                   aTTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGGATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGGTGATCCCGGGTTTGGGGGACTGGCTGGGAGGGATGGCGCTTGCGGGGGTTTG‑C‑TTTTTTTCTGGCGGGCCgggggattttgcatgggctc                            >  2:19161/1‑234 (MQ=255)                                                                                                                                                                                                                      gggtgTTGGCAAAAACGCGGCGCTGGGGATCGGGTTGATTAGCTTGTTCTTTATGCTCGGTTGTATTCCTCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACTCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTTTCTCTGGCGTGCCGGGTAATTTGCAGTGGCTCTATc                         <  2:560773/247‑1 (MQ=255)                                                                                                                                                                                                                              gCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGGTGATCCCGGATATGGGGATCTGGCTGGAAGTGATGGCCTTTGCGGGG‑TATG‑CCTTTTTCTCTGGCGGGCCGGGGGATTTGCAGTGGCTCTATCCTAATgg                  >  1:465329/1‑249 (MQ=255)                                                                                                                                                                                                                                            ctggggAACGTGGTGATTAGCCTGTTCTTTAAGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGATTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCGGATACCGGACTGGGGGATCCGGGTGGTGGGGATGGACTTTGAGGGG‑GAAG‑CCGTTTTTTCTGGCGGGCCGGGGCATTTGCAGAGGCTCTATCCCAAttgaatattcccggac     >  1:146893/4‑234 (MQ=255)                                                                                                                                                                                                                                             tggggAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGGAGTGATGGCCTTTGCGGTG‑GATG‑CCTTTTTTTCTTGCGGGCCGGGGAATTTGCCGTGGCTCTATCCTAATGAACTCTTCCCGCCAGa   >  1:520398/3‑250 (MQ=255)                                                                                                                                                                                                                                              ggggAACGTGGTGATTAGCCTGTTCTTTATGCCCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTTGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGat  <  1:238618/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTG‑TATG‑CCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGAT  >  NC_000913/46418‑46904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑