| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 45,978 | C→T | L58L (CTG→TTG) | yaaU → | putative transporter YaaU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 45,978 | 0 | C | T | 96.7% | 166.4 / ‑4.0 | 61 | L58L (CTG→TTG) | yaaU | putative transporter YaaU |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (27/31); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
GTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGG‑ACGCTGACTGGAT‑TGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGT > NC_000913/45735‑46206 | gTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTACAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGgcgc > 1:120487/1‑251 (MQ=255) cggctgagtggggTTTGATTCGTAACGCCGAACTGACTCTCATTGCAAAAACCAGGAATAACCAGGCAACCGTCCAGAAACTTGGACGACCTCAAATTCCCCTCTATTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCt < 2:639907/240‑1 (MQ=255) gtcTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGG‑ACGCTGACTGGAT‑TGGCTTGCTGGGCGCGGGAACGCTc > 1:212958/3‑251 (MQ=255) ggCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTc > 1:350461/1‑251 (MQ=255) tgctGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGTT‑TGGCTTGTTGGGCGCGGGaagtgccgcc > 2:209393/4‑243 (MQ=255) gTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAATTTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGG‑ACGCTGACTGGAT‑TGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGtt < 1:187179/251‑1 (MQ=255) aCGTACCGCCGCATTGACTCTCATTGCAAAAAACAGGAATACCCAGACACCCGTCCAGAAACTTTAACGATCTCAAAGTCTCCTTTATTCACCGCCGCATTTTGCTGTGGGGCAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTATAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCTGGAACGCTCGCCGGGCTTTTCGTTGGc < 2:277773/251‑1 (MQ=255) cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGCATAACCATGCAACCGTCCAGACACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGCTGGATATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAACACTGG‑ACGCTGTCTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGcc > 1:51375/1‑250 (MQ=255) cgcggcACTGCCTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCATAAACTTTGACGATCTCAAATTCTCCTCTATTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGTCTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg < 1:176663/247‑1 (MQ=255) tctcATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCtat > 1:63072/1‑251 (MQ=255) ctcATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTggcta < 1:204474/249‑4 (MQ=255) cATTGCAAAAAACAGGCATAACCATGCAACCGTCCAGAAACTTTGACGATATCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCACCTGACGCCGGCGCTAAAACTGG‑AAGCTGCCTGGAT‑TGGCATGTTGGGAGCGGGAACGCTCGACAGGCTGTTCGTTGGCACCACGCTGTTTGGCTATAtt > 2:658930/1‑251 (MQ=255) gCAAAAAAAGGGAATACCCAGGCAACCTCCCAGAAATTTTGTCTATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAATCTGG‑ACGCTGACTGGAT‑TGGCTTGTTTGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGGACATCGCTGTTTGGCTATATTTcc < 2:263750/250‑1 (MQ=255) acAAAAAACAGGAATCACCATGCAACCGGCCAGAAACTGGGGCGATCCCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAACCTGG‑ACGCTGACTGGAT‑TGGCGTGTTGGGCGCTGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCg < 2:337842/250‑1 (MQ=255) cAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCCGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGa < 1:260723/251‑1 (MQ=255) aacaaaCAGGAATAACCATGCAACCGTACAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTACTGTGGGGAAGCGGTGGTCAGTTTCTGGATGGTTATGTACTGGTAATGATTGACGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGTCGCGGGAACGCGCGCCGGGCTGTTCGTTGGCACATCGCTGTGTGGCTATATTTCCGAt > 2:265377/4‑251 (MQ=255) aagaaaTAACCCTACAACCTACCAGAAATTGTGACGATGTAAAATTCCCCTCTAGTCACCGCCGCATTGTGCTGTGGTGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACGTGTAATGATTGTCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGTAT‑TGGCTTGTTGGGCGCGTGAACGCTCGCCGGGCTGTTCGGTGGCACATCGCTGTTTGGCTATATTTCCGATAAAggc < 2:329163/247‑3 (MQ=255) aaCCATGCACCCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCTGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgc < 1:647807/251‑1 (MQ=255) aCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgcg < 1:267224/251‑1 (MQ=255) ccATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgcgc > 1:328098/1‑251 (MQ=255) cGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGCCGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACCTCGCTGTTTGGCTATATTTCCGTTAAAGTCGGCCGGGTCCAAATGTTc > 2:109080/1‑251 (MQ=255) ccagtaatgttGAAGATCTCAAATTCTCCTTTTTTCACTGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGGACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGTAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTc < 2:407434/242‑1 (MQ=255) ttGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATatc > 1:640623/1‑251 (MQ=255) gtgacacaatgtctcCTTTATTCACCGCCGTTTTTTGATGGGGGGAGGCGGTGGCCCGTTTCTGAATGGTTATGTACTGGTAATGATTGGCGTGGCGTTGGAGCAACTGACGCCGGCGCTAAAACTTG‑TCGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAGAGCTCGTGGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAATGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCg < 2:227070/240‑1 (MQ=255) cGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGCGGTCCGTTTCTGGATGGTTATGTACTGGAAATGATTGACGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATACAGTCGGACGGCGCAAAATGTTCATCATTGATATCATCg > 1:664253/1‑251 (MQ=255) aTCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGGCGGCGCAAAATGTTCCTCATTGATATCCTCGcc > 2:48141/1‑251 (MQ=255) tcAAATTATCCTCTATTCACCGCCGAGTTTTGCTGTGGGGACGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGCAATGTTTGGAGTGGCGCTGGCGCAAATGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGATCGGACCGGGCACAATCGCCGGGCTGTTCGTGGGCACATGGCTGTTTGGCTCAATTTCAGTCTAAGTCGGGCGGCGCGAAATGTTCCTCATTGTTatcttctacatc > 2:627187/1‑242 (MQ=255) cAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATc < 1:64839/250‑1 (MQ=255) aaaTTCTCCTCTATTCCCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCgg < 1:673696/251‑1 (MQ=255) tATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGatattgg > 1:603062/1‑248 (MQ=255) ttCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCAGCGGCGTGATATCGGt < 1:594180/250‑1 (MQ=255) ccgcATTTTGCTGTGGGGGAGCGGTGGACCGATTCTGGATGGTTATGAACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTAG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGGACGCTCGCCGGGCTGTTCGTTGACCCATCCATGTTTGACTATATTTCCGATAAAGTCGCACGGCGCACAATGTGCCTTATTGATAACATCGCCATCGGCGTGATATCGGTggagctgg > 2:428597/1‑245 (MQ=255) gcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TTGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGAtg < 1:635243/251‑1 (MQ=255) gcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCAGCGCCATCGGCGTGATATCGGTGGCGACGAtg < 1:536161/251‑1 (MQ=255) ttttGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTGCTGGTAATGATTGGCGTGGCGCTGCAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGcgcg < 1:660018/125‑1 (MQ=255) tgtgGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGGGATATCGGTGGCGACGATGTTTGTTTc > 1:607070/1‑250 (MQ=255) aaGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCg < 1:48141/251‑1 (MQ=255) gtgtgccttttCTGGATGGTGATGTGCTGGGAATGATTGTAGTGGCGCTGTAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGTCTGGAT‑TGGCTTGTTGGGCGCGGGAACTCTCGCCTGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGTACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATGGGTGGCGACGATGTTTGTTTCATCCCCCGTCg < 2:470229/241‑1 (MQ=255) ggtCCGTTTCTGGATGGTTATGGACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGCTATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGacct > 1:24812/1‑248 (MQ=255) gtCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTg < 1:486230/251‑1 (MQ=255) ttCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGGTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCTCCATCGGCGTGATATCGGTGGCGGCGATGTTTGTTTCATCCCCCGTCGGACtttttgtt > 2:178434/1‑244 (MQ=255) tCTGGATGGTTATGTACTGGTAATGATGGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGCT‑TGGCTTGTTGGGCGCGGGAACGTTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCTGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCTCGTCGTACTGTTgggg < 2:640623/250‑3 (MQ=255) tggtaggTTATGTTCTGGTAACTATGTGCGTGGCGCTGGAGCAACTTACGCCGGTGCT‑AAATTGGGACTCGGGCTTGATGTGG‑TTGTTGGGCGCGGGAACGCTAGCCGGGCTGTTCGTTGGCACTTCGCTGTTTTGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATGGATATCAGCGCCATCGTCGTGATAGCGGTGGCGACGATGGGTGTTTCGGCCCCCGTCGTACTGGTGGTGAt < 2:278452/245‑1 (MQ=255) aTGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCt < 1:514089/127‑1 (MQ=255) aTGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGAAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGCT‑TGGCTTGTTGGGCGCGGGGACGCGCGCCGGGCTGTTCGTTGGCACATCGCt > 2:514089/1‑127 (MQ=255) ggTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCggg < 1:455094/250‑1 (MQ=255) tGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACtt > 1:415308/1‑250 (MQ=255) aaTGATTGGAGTGGCGATGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGCT‑TGGCTTGTTGGGCGCGGGAACGATCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGACGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGTCGATGTTTGTTTCATCCCCCGTCGGACTGTTGGTGGTGCGGGTACTTATCGGCCTTg > 2:84966/1‑251 (MQ=255) gATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCa < 1:530266/251‑1 (MQ=255) gtgacgcTGGCGCAACTGACGCCGGCGCTAAAACTGG‑ACGCTGACTGGCT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTACGTTAAAGTCGGTCGGCGCAAAATGATCCTCCTTGATATCATCGCCATCGGCGTGAAATAGGTGGCGACGATGTTTGTTTTATCCCCCCTCGTACTGTTGTGGATCCGGGTACtttttagcattgtgctaggtgc > 2:77043/5‑231 (MQ=255) ggggcgCGGGATCAACTGACGCCGGCGGTAAAGCGGG‑ACGCTGACGGGAT‑TGGCTTGTTGGTCGCGGGATCGCTCGCTGGGCTGTGCGTTGGCACATCGCTTTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGTCGATGTTGGTTTCATCCCCCGGCGAACTGTGGGTGATGCGGGTACTTAACGGCATTTTCATCGGTGc < 2:239256/249‑1 (MQ=255) aCTGACGCGGTCGCTAAAACGGG‑ACGCTGACTGGAT‑TTGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTGCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGGTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCg < 2:328098/251‑1 (MQ=255) aCGCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCAc > 1:98193/1‑251 (MQ=255) gCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGTTATCATCGCCATCGGCGTGATATCGGGGGCGGCGATGTTTGTTTTCTCCCCCGTCGAACTGTTGGTGCTGCGGGGACTTATCTGCCTAGTCATCGGTACAGCTTATCCCATcccccccc > 2:356385/1‑244 (MQ=255) gCCGGCGCTAAAACTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCt > 1:478774/1‑251 (MQ=255) aaaaCTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCa < 1:84966/250‑1 (MQ=255) tagcTGG‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAACTGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGGTGTTTGTTTCATCCCCCGTCGAACTGTTGTGGATGCGGGTACTTATCGGCGTTGTCATCGGTGCAATTTATCCCATCGCACCCTCAATGAtaac > 2:504944/4‑248 (MQ=255) aCTGG‑GCGCTGCCTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTGCGTTGGCACATCTCTGTTTGGCTATATTTCCGATAAAGGCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGAGTATCCCATCGCCACCTCAATGATCACCg < 2:350461/251‑1 (MQ=255) gg‑ACGCTGACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATCTCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGGGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCTCCTCatagctcacagtgt > 2:178085/1‑238 (MQ=255) gacgggAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGGTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTAc < 2:87788/183‑1 (MQ=255) gACTGGAT‑TGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTAc > 1:87788/1‑187 (MQ=255) | GTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGG‑ACGCTGACTGGAT‑TGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGT > NC_000913/45735‑46206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |