Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 27,104 G→A Q276Q (CAG→CAA)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091327,1040GA99.0% 315.0 / NA 101Q276Q (CAG→CAA) ispH4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (38/62);  total (39/62)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATT  >  NC_000913/26859‑27349
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
gACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTACGGATATTCTG‑GTGCAAAAtgt                                                                                                                                                                                                                                                   >  1:653221/1‑251 (MQ=255)
      tGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAAtg                                                                                                                                                                                                                                                    <  1:153498/244‑1 (MQ=255)
        cTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCCGCGTATGGGCAAACGCGCGTTTTTG‑CTTGACGATGCGAAAGATATACAGGAAGAGTGGGTGCAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCAc                                                                                                                                                                                                                                           >  1:423359/1‑251 (MQ=255)
            gCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGCAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGttt                                                                                                                                                                                                                                       >  1:509950/1‑251 (MQ=255)
              cACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACCGGCGAAAGATGTTTTGGTGGACGGTTCCAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGATGGCCCAGCGTATAGACAAACGAGCGTATTTGCATGGA‑GATGAGCACGATATCCAGGAAGAGTGGGTGCAAGAGGTTAAAAGCGTCGGAGTGACTCCGGGAGCATCAGCCACGCATATTCTG‑GTGCAAAACGTGGAGGCCCGTTTgc                                                                                                                                                                                                                                     >  2:440505/1‑251 (MQ=255)
                 gACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTgcagc                                                                                                                                                                                                                                  >  1:475633/1‑251 (MQ=255)
                    tAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCagcagc                                                                                                                                                                                                                               >  1:358/1‑251 (MQ=255)
                      aCCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTg                                                                                                                                                                                                                             <  1:653230/251‑1 (MQ=255)
                       ccGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCTGAAGTTGTGGTGGTTGTCGGTTCGAAAAACTCCTCCAACTCCAACCTTCTGGCGGCGCTGGCCCAGCGTATGGGCAAGCGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCATGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGTGTGACTGCGGGCTCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCTCGTTTGCAGCAGCTgg                                                                                                                                                                                                                            <  2:416805/251‑1 (MQ=255)
                       ccGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTgg                                                                                                                                                                                                                            <  1:307830/251‑1 (MQ=255)
                         gTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTTGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGc                                                                                                                                                                                                                          <  1:384778/251‑1 (MQ=255)
                          gcAGGAAGCGGTACGCGACCTGGCAGAACAGGCGGCAGGTGTTTTGGTGGTCGGTTCGACAAACTCCCCCAACTCCAATCGTCTGGAGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCTGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGCCTGCGGGCGCATCGGCTCCGGATATTCTT‑GTGCAAAATGTGGTTGCACGCTTGCAGAAGGTGGGCg                                                                                                                                                                                                                         <  2:483803/250‑1 (MQ=255)
                               aaGCGGAACGCGCCCTGGCAGAACAGGGGGAAGTTGTGTTGGGGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGGTGGCCCAGCGTATGGGCAAACGCGCGTTTGTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGAGTGACTGCGGGCGCATCGGCTCCGGATAGTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGTTGGGCggtggt                                                                                                                                                                                                                    <  2:100858/251‑1 (MQ=255)
                                   ggTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGcagcag                                                                                                                                                                                                                                >  1:402646/1‑235 (MQ=255)
                                   ggTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACGCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCTAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTTCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGcagcag                                                                                                                                                                                                                                <  2:402646/235‑1 (MQ=255)
                                         cgcCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTGCAACTCCAACCGTCTGGCGGAGCTGGCACAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGACAGATATCCAGGAAGCGGGGGTGACAGAGGTTAAATGCGTAGGCGAGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGGGGCGGTGAAGCCATTc                                                                                                                                                                                                          >  1:427803/1‑251 (MQ=255)
                                            ccTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGATTGCAGCAGCTGGGCGGGGGTGAAGCCATTCCGc                                                                                                                                                                                                       >  1:497459/1‑251 (MQ=255)
                                              tGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTg                                                                                                                                                                                                     >  1:83010/1‑251 (MQ=255)
                                               ggCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCt                                                                                                                                                                                                      <  1:576564/249‑1 (MQ=255)
                                                    aaCAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAACACTCCTCCAACTCCAACCGACTGTAGGAGCTGGCCCAGCGTATGGGCACAAGCGCGTTTTTG‑ATTGACGATGCGAAAGATATACAGGAAGAGTGGGAGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGGATCGGCACCGGATATTCTG‑GAGCAAAATGAGGTAGAGAGTTTGAAGCAGCTGGGCGGTGGTGAAGACATTCCGCTggcag                                                                                                                                                                                                 >  2:334679/1‑246 (MQ=255)
                                                           ggAAGTTTTGTGGGTGGTCGGTTCGAAAATCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGTTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTgaag                                                                                                                                                                                        <  2:555848/251‑1 (MQ=255)
                                                             aaGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCACCTACCACCGTCGGGCTGAGCTGTCACAGCGTATGGGAACACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGACGATTGGGTGAAATAGGCTAAATGCGTTGTCGTGACTGCGGGCGCCTCGGCTCCGGATATTCTG‑GTGCAAAATGTTGTGGCAAGCTTGCAGTCGCTGGGCGGTGGTGAAGTCCTTCCGCTGGAAGGCCGTgtagaa                                                                                                                                                                                      <  1:413056/251‑1 (MQ=255)
                                                                    gtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACAtt                                                                                                                                                                                <  1:465199/250‑1 (MQ=255)
                                                                    gtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACAtt                                                                                                                                                                                <  1:61434/250‑1 (MQ=255)
                                                                             cGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAg                                                                                                                                                                      <  2:571500/251‑1 (MQ=255)
                                                                              ggTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGt                                                                                                                                                                     <  2:524423/251‑1 (MQ=255)
                                                                               gTTCGAAAAACTCCTCCGACTCCAGCCGGCTGGCGTAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCTGCGTGACTGCGTGCGCATCGGCTCCGGATTTTCTG‑GTGCAAAATGTGGTGGCACGGTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTg                                                                                                                                                                    <  1:436359/251‑1 (MQ=255)
                                                                               gTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCTGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGTTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTg                                                                                                                                                                    <  1:423636/251‑1 (MQ=255)
                                                                               gTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGAGCAAACGCGCGTTTTTG‑ATTGACGATGCGAGAGATATCCAGGAAGAGTGGTTGAAAGAGGTTAAATGCGTCGGCGGGACTGCGGGCGCATCGGCTCCGGCTATTCTG‑GTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGGGACGCCATTCCGCTGGAAGGCCTTGGAGAAAACGTTGTTTTCGAAGTg                                                                                                                                                                    >  1:524375/1‑251 (MQ=255)
                                                                                     aaaaCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGaaa                                                                                                                                                              <  1:239648/251‑1 (MQ=255)
                                                                                        aCTCCTCCACCTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAagag                                                                                                                                                           <  1:137683/251‑1 (MQ=255)
                                                                                        aCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAagag                                                                                                                                                           <  1:204957/251‑1 (MQ=255)
                                                                                             tccaactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCg                                                                                                                                                     >  1:386927/1‑251 (MQ=255)
                                                                                              ccaactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCgt                                                                                                                                                    <  2:45236/251‑1 (MQ=255)
                                                                                                aactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCgtgt                                                                                                                                                  <  1:452402/251‑1 (MQ=255)
                                                                                                 actccaacTGTCGGGCGGTGTGGGTCCAGCGTATGGGTAATCGCGTGTTTGTG‑ATTGACGAGGCGAAAGATATCCTTGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCTGGACTGCTGGCTCATCGGTGCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGATGGCCGTGCAGAAAACATCGTTTTCGACGTGCCGAAAGAGC‑TGTGTGTc                                                                                                                                                 <  2:22774/251‑1 (MQ=255)
                                                                                                 actccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCTCGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTc                                                                                                                                                 <  1:680059/251‑1 (MQ=255)
                                                                                                 actccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTGTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTc                                                                                                                                                 <  1:283442/251‑1 (MQ=255)
                                                                                                     caacCGTCTGGCGGAGCTGGCCCAGCGTACGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGATGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGGGGTGGCACTTTTGCAGCAGCTGGTCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGata                                                                                                                                             <  2:36942/251‑1 (MQ=255)
                                                                                                        ccGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTc                                                                                                                                          <  1:445459/251‑1 (MQ=255)
                                                                                                         cGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCg                                                                                                                                         <  1:294965/251‑1 (MQ=255)
                                                                                                             tGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGCTATTCGTGa                                                                                                                                      >  2:325623/1‑250 (MQ=255)
                                                                                                              ggCGGAGCTGGCCCAGCGTATGGGCAATCGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAg                                                                                                                                    <  1:433171/251‑1 (MQ=255)
                                                                                                              ggCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTg                                                                                                                                       <  1:70327/248‑1 (MQ=255)
                                                                                                                       ggCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAg                                                                                                                           <  1:588877/251‑1 (MQ=255)
                                                                                                                         cccAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTc                                                                                                                         <  1:325623/251‑1 (MQ=255)
                                                                                                                             gCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTa                                                                                                                     <  1:561390/251‑1 (MQ=255)
                                                                                                                               gTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGACGAGTGGGTGAAAGAGGTTACATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGACGTGCAGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagc                                                                                                                   >  1:324369/1‑251 (MQ=255)
                                                                                                                                 aTGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTg                                                                                                                                                                                                                                      <  2:139027/135‑1 (MQ=255)
                                                                                                                                 aTGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTg                                                                                                                                                                                                                                      >  1:139027/1‑135 (MQ=255)
                                                                                                                                 aTGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcag                                                                                                                 <  1:30656/251‑1 (MQ=255)
                                                                                                                                 aTGGGCAAACGCGCGTTTTTG‑ATTGACGAAGCGAAAGATATCCAGGAAGAGTGGGAGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcag                                                                                                                 <  2:260109/251‑1 (MQ=255)
                                                                                                                                  tGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcagc                                                                                                                >  1:118765/1‑251 (MQ=255)
                                                                                                                                     gCAAAAGCGCGATTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGCGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAACATGAGGTGGAACGCTTGCAGCAGCTGGGCGGTGGTGAAGACATTACGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCGTG‑GCGTCGATATTCGTGAAGCTGCTTGCGTCATTAGCAGCCt                                                                                                              >  2:133497/1‑250 (MQ=255)
                                                                                                                                      cAAACGCGCGTTGTTG‑AATGATGATGCGAAAGATATCCAGGAAGAGTGGGGTAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTTGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTaa                                                                                                            <  1:445615/251‑1 (MQ=255)
                                                                                                                                        aaCGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGt                                                                                                          <  1:633690/251‑1 (MQ=255)
                                                                                                                                          cgcgcgTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGCGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGTGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTTAAGAAAACATTGTTTTCGACGTGCCGGTAGAGC‑TGCGTGTCGATATTCGTGACGTCGATTAAGGCATTAGCCGCCTAAGtt                                                                                                         >  2:126214/1‑250 (MQ=255)
                                                                                                                                             gcgTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGAGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGAGTCGATCTTCGTGACGTCGATTAAGACATTAGCAGCCTAAGTTATGc                                                                                                     >  1:618280/1‑251 (MQ=255)
                                                                                                                                             gcgTTTTAG‑ATTGCCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCCGGCGCATCGGCTCCGGATATTCTG‑GTGCAAACTGTGGTGGCGCGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGACGTGAAGAAAACATTGTTTTCGAAGTGACGAAAGAGC‑TGCGTGTCGATATTCGTGTCGTCGATTCAGTCATTAGGCGCCTACGTTATGc                                                                                                     >  2:85147/1‑251 (MQ=255)
                                                                                                                                                 ttttG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGaa                                                                                                  >  1:415356/1‑250 (MQ=255)
                                                                                                                                                   ttg‑attgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGaaaa                                                                                                <  1:309022/250‑1 (MQ=255)
                                                                                                                                                      gatggaCGATGCGAAAGATATCCAGGAAGAGGGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCACCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAATCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATg                                                                                              <  2:361665/246‑1 (MQ=255)
                                                                                                                                                       attgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGcc                                                                                            >  1:533009/1‑251 (MQ=255)
                                                                                                                                                        ttgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCg                                                                                           >  1:429558/1‑251 (MQ=255)
                                                                                                                                                            cGATTCGAAAGATGTCCAGGAAGAGTGGGTGAAAGAGGTTAATTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGCTCTTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATGCCGCTGGAATGTCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCACTAGCAGCCTAAGTTATGCGAAAATGCCGGTCt                                                                                       <  2:328650/251‑1 (MQ=255)
                                                                                                                                                             gATGCTAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGGGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAAGGTGGTGGCACGGTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCtt                                                                                      <  1:635216/251‑1 (MQ=255)
                                                                                                                                                                  gAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTCGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAc                                                                                 >  2:180762/1‑251 (MQ=255)
                                                                                                                                                                       ataTCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGc                                                                             >  1:577499/3‑250 (MQ=255)
                                                                                                                                                                               gaagcgagggTGAAAGAGGTTAAATGCGTCGGCGTGCCTGCGGGCGCATCGGCTCCGGATATTCTG‑GAGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGGGGTCAAGCCCTTCAGCTGGAAGGCCGTGACGCAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGACGCTATTCGTGAAGTCGATTACGTCCTTAGAAGCCTATGTTATGCGAAACTGACGGTCTTGTTACAGACATTTTTTAt                                                                    >  2:406582/8‑251 (MQ=255)
                                                                                                                                                                                  gagTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAAGGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGga                                                                 <  1:494171/251‑1 (MQ=255)
                                                                                                                                                                                   agTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGgag                                                                <  1:176608/251‑1 (MQ=255)
                                                                                                                                                                                   agTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATGCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGACGAAAGAGC‑GGCGTGTCGATATTGGTGAAGTCGATTAAGGCATTAGCAGCCCAAGTTATGCGAAAATGCCGGGCTTGTTACCGGTATTTTGTATGgag                                                                <  2:653221/251‑1 (MQ=255)
                                                                                                                                                                                    gTGGGTGAAAGATGTTAAATGCGTCGGCGTGACTGCGGGCGCTTCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTTGTGAAGCCATTCCGTTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGTTTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGTCATTTTTTATGgaga                                                               <  2:497459/251‑1 (MQ=255)
                                                                                                                                                                                    gTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGgaga                                                               <  1:15672/251‑1 (MQ=255)
                                                                                                                                                                                    gTGGGTGAAAGAGGTTAAATGCGACGGCGGGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCCGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGCGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGACGCCTCAGTTATGAGCAAATGCCGGTCTTTTTACCGGCTTTTTTTATGgat                                                                >  2:19496/1‑249 (MQ=255)
                                                                                                                                                                                     tGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGAATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGACTTGTTACCGGCATTTTTTATGgaga                                                               >  1:299735/1‑250 (MQ=255)
                                                                                                                                                                                      gggTCAAAGATGTTAAAGGGGTCGGCTTGTCTGCCTGCGTATCGGCTCCGGATATT‑TGTGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGACATTCGGCTGGAAGGACGTGAAAAAAACATTGATTGCGAAGTGCCGAAAGAGC‑TGCGTGTCGAGATTCGTGAAGTCGATTAAGTCATTAGCAGACGAAGTTATGCGAAAAAGCCGGTCTTGTTACCGGCATGGTTTATGGAGaa                                                              <  2:511134/250‑1 (MQ=255)
                                                                                                                                                                                          gAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACa                                                          <  1:132484/250‑1 (MQ=255)
                                                                                                                                                                                          gAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACAt                                                         <  1:239534/251‑1 (MQ=255)
                                                                                                                                                                                            aaGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGc                                                       >  1:196198/1‑251 (MQ=255)
                                                                                                                                                                                             agggtttaaatggcaccgcgagACTGCGGTCGGATGGGCTTCGGATAGTCTG‑GTTCAAAATGTGTTGTCACGATTGCAGCAGCTGTGGGGAGGTGAAGCCCTTACGCTGGAAGGCCGTGAAGAAAACATTGGTTTCTAAGTGCCGAAACAGC‑TTCGTGTCGATATTCGGTAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCTGTCTTGTTACCGGCATTTTTTATGGAGAAATCATGCg                                                      <  2:194208/230‑1 (MQ=255)
                                                                                                                                                                                               tggTTAAATGCGTCGGCGTGACTGCGGGCGCATCTGCTCCGGATATTCTG‑GTGCAAAACGTGGTGGCACGTTTGCAGCAGCTGGACGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGCAGAAAACATTGTTTTAGATGTGCCGAAAGAGC‑TGCGTGTCGATCTTCGTGAAGTCGATTAAGTCCTTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCggt                                                    >  2:313064/2‑249 (MQ=255)
                                                                                                                                                                                                ggTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGaaaa                                                                                                >  2:269634/1‑206 (MQ=255)
                                                                                                                                                                                                ggTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGaaaa                                                                                                <  1:269634/206‑1 (MQ=255)
                                                                                                                                                                                                  ttAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGAGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGGGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTCAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGGGTTTAc                                                 >  2:16135/1‑251 (MQ=255)
                                                                                                                                                                                                    aaaTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCt                                               >  1:495528/1‑251 (MQ=255)
                                                                                                                                                                                                     aaTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTAGGCGGGGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAAAATGCGCTTACCt                                               >  1:277061/1‑250 (MQ=255)
                                                                                                                                                                                                          gTCGGAGTGACTGCGGGCACATCGGCTCGGGATATTATG‑GAGAAAAATGTGGCGGCACGTTTGCAACAGCTGGGCAGTGGTGAAGACATTCCGCTGGAAGGCCGTGAAGACAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGGCGGTATTCGTGGCGTCGATTAAGTAATTAGCAGCCTAAGTTATGCGAAACAAAAGGTCTTTTTACAGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTc                                         >  2:223628/1‑251 (MQ=255)
                                                                                                                                                                                                            cGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAATCATGCGTTTACCTATCTTcc                                        <  2:577499/250‑1 (MQ=255)
                                                                                                                                                                                                               cGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCg                                     >  1:330265/1‑250 (MQ=255)
                                                                                                                                                                                                                gTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGCTATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAACTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAt                                             >  2:641876/1‑241 (MQ=255)
                                                                                                                                                                                                                gTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAt                                             <  1:641876/241‑1 (MQ=255)
                                                                                                                                                                                                                          gcgctacGGCTTCGGATATTCGG‑GTGCAAAATGTGGTGGCACGTTTGCATGAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCg                         <  2:358/245‑1 (MQ=255)
                                                                                                                                                                                                                           gtcATCGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATGCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGGCATTAGCAGCCTAAGTTATGCTAAAATGCCGGTCTTGTTACCGGCATTTTTGAGGGAGAAAACATGCGGTTACCTATCTTCCTCGATACTGACCCCgg                        <  2:116228/249‑1 (MQ=255)
                                                                                                                                                                                                                                cGGCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTg                   >  1:477231/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ggCTCCGTATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTTAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGTCTAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGa                  <  1:413404/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gCTCCGGATATTCTG‑GTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTGTACCTATCTTCCTCGATACTGACCCCGGCATTGAc                 <  1:510899/251‑1 (MQ=255)
                                                                                                                                                                                                                                           atTCTT‑GTGCAAAATGGGGTGGCACGTTTGCAGCAGCTGGGCGTTTGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAATCATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCTTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc        <  2:441197/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTGCAAAATGTGGTGGCCCGTTTGCAGCAGTTGGGCTGGGGTGACGCCAGTCCGCTGGATGGCCGGGAAGAAAACAGTGTTTTCGAAGTGCCGAAAGATC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCTGAAGTTATGCGAAAATGCCGGTCTTGTTTCCGGCATTTTTTATGGAGAAAACATGCGTGTACCTATCTTCCTCGATACTGTCTCCGGCATTGACGATTTCGGCGCCAtt  <  2:423359/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGGCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCGCGATACTGACCCCGGCATTGACGATGCCGTCGCCAtt  <  1:222640/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
GACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTG‑GTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATT  >  NC_000913/26859‑27349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑