| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 27,026 | C→T | D250D (GAC→GAT) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 27,026 | 0 | C | T | 98.9% | 272.1 / ‑4.3 | 94 | D250D (GAC→GAT) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (40/53); total (40/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01 | |||||||||||
CGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAC > NC_000913/26779‑27270 | cGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGCACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGCCGATGCGAAAGATATc > 1:648313/1‑251 (MQ=255) tCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAgg > 1:361665/1‑250 (MQ=255) ggTGGATGAAACGGCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACGCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAag < 2:645707/251‑1 (MQ=255) aTGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTgg < 2:223947/251‑1 (MQ=255) gATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAgg < 1:519151/250‑1 (MQ=255) gATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAAt < 1:239633/251‑1 (MQ=255) aTCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTCACCGTCAGGAAGCGGTACGCGCCCTGGCAGACCAGGCGGAAGTTGTGTTGGTGGACGGTTCGAAAACCTCCTCCACCTCCACCCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGCTGCGAACGATATCCAGGACGAGTGGGTGACAGAGGTTAAATg > 2:70327/1‑251 (MQ=255) cgcgCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCg > 1:671525/1‑251 (MQ=255) catAAAAGCTTCCCGAAAATTGTCGGTCCGCGAAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGACGGGTCAAAAAACTACACCAACTCCAAACGTCTGGAAGCGCTGGCGCAGCGTATGGGCAAAAGCGCTTTTTTG‑TTTGACGATGCGAAAGGTATCAGGGAAGCGAGGGTGAACGTGGTTAAATGAGACGGCGtaac > 2:429225/3‑248 (MQ=255) gTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACACCTGCTTCGCCACGACTAACCGTCAGGAAGCGGTACGTGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACGCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGGTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACt < 2:398895/251‑1 (MQ=255) aaaCGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGc < 1:668595/251‑1 (MQ=255) aaCGCTTCCAGAAAATTGTTGGTCCGCGCACAGATGACATCTGCTCCGCCACGACTCACCGTCAGGAAACGGTACGCGCACTGGAAGAACAGGCGTCAGGTCTGATGGAGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGCATGGCGGCGCTGGACCAGCGAATGGGCAAACGCCCGTTTTTG‑ATTGACGCTGCGAACAATCTCCAGGAGAAGTGGGTGAAAGAGGCTAACTGCGACGGctcgacagcg > 2:341547/1‑242 (MQ=255) aaaTTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCCGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGAGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGcc > 1:617231/1‑250 (MQ=255) aaaTTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCt > 1:45236/1‑251 (MQ=255) aaaTGGTCCGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGGCAGGAAGCGGTACGCGACCTGGCAGAACAGGAGGAAGTTGTGTTGGTGGTCGGTTCGAAAATCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCt < 2:78523/251‑1 (MQ=255) attgtaggtcagcgacacaattaCATCTGCTACGCCACGACTACCCGTCAGGCAGCGGTACGCTCACCGGCAGAACAGGAGGACGCTGTGTCGGCGGTCGGTTCGAAAACCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCCCCGTATGGGAACACACGCGTATTTG‑ATTGTCGATGCGAAAGATATCCAGGGAGAGCAGGCGAAAGGGGTTAAATGCGTCGGGGTGCATGCGGGCGCAACGGCtgc > 2:626639/23‑249 (MQ=255) cGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGata < 1:574968/251‑1 (MQ=255) cGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGata < 1:215164/251‑1 (MQ=255) ccGCGCAAAGATTACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGGACGCGACCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACGGCGGGCGCATCGGCTCC‑GGATATTCt < 2:630571/251‑1 (MQ=255) cgcAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGACCAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTTGCCCAGCGTATGGGCAAACGCGCGCTTTTG‑TTTGAAGAAGCGAAAGATATCCAGGAAGACGGGGCGAAAGAGGTAAAATGCGTCGGCGTGACTGCGGGGGCATCGGCTCC‑GGGTTTTCTggg > 2:222640/1‑250 (MQ=255) aaGATGACATCTGCTACGACACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCa > 1:116228/1‑250 (MQ=255) aTGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAGACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCCGCGTATGGGCAAACGCGCGGTTTTT‑ATTGACGATGCGAAAGATATCCAGGAAGAGGGGGGGCAAGAGGTTAAATGAGTCGGCGGGAATTGGGGCGGATCGGCTCC‑GGACATTcggttgcaaaat > 2:510899/1‑240 (MQ=255) gACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTAC‑GGATATTCTGGTGCAAAAtgt > 1:653221/1‑251 (MQ=255) cTGCTACGACACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACCGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCAGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATACAGGAAGAGTGGGAGGAAGAGGTTAAATGCGTCGGCGTTACTGCGGGCGCATCGGCTCC‑GGATATTCAGGTGCaagatggggtgg > 2:176608/1‑240 (MQ=255) tGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGCAGTTGTGTTGGTGGTCGGTTCGCAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAACCGCGCGTTTTTG‑CTTGACGATGCGAAAGATATCCCGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGAATGCGGGCGCATCGGCCCCCGGatatctggcgcaaaattggatcgg > 2:153498/1‑231 (MQ=255) tGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAAtg < 1:153498/244‑1 (MQ=255) cTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCCGCGTATGGGCAAACGCGCGTTTTTG‑CTTGACGATGCGAAAGATATACAGGAAGAGTGGGTGCAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCAc > 1:423359/1‑251 (MQ=255) gCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGCAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGttt > 1:509950/1‑251 (MQ=255) cACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACCGGCGAAAGATGTTTTGGTGGACGGTTCCAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGATGGCCCAGCGTATAGACAAACGAGCGTATTTGCATGGA‑GATGAGCACGATATCCAGGAAGAGTGGGTGCAAGAGGTTAAAAGCGTCGGAGTGACTCCGGGAGCATCAGCCAC‑GCATATTCTGGTGCAAAACGTGGAGGCCCGTTTgc > 2:440505/1‑251 (MQ=255) gACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTgcagc > 1:475633/1‑251 (MQ=255) tAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCagcagc > 1:358/1‑251 (MQ=255) aCCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTg < 1:653230/251‑1 (MQ=255) ccGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCTGAAGTTGTGGTGGTTGTCGGTTCGAAAAACTCCTCCAACTCCAACCTTCTGGCGGCGCTGGCCCAGCGTATGGGCAAGCGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCATGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGTGTGACTGCGGGCTCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCTCGTTTGCAGCAGCTgg < 2:416805/251‑1 (MQ=255) ccGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTgg < 1:307830/251‑1 (MQ=255) gTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTTGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGc < 1:384778/251‑1 (MQ=255) gcAGGAAGCGGTACGCGACCTGGCAGAACAGGCGGCAGGTGTTTTGGTGGTCGGTTCGACAAACTCCCCCAACTCCAATCGTCTGGAGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCTGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGCCTGCGGGCGCATCGGCTCC‑GGATATTCTTGTGCAAAATGTGGTTGCACGCTTGCAGAAGGTGGGCg < 2:483803/250‑1 (MQ=255) aaGCGGAACGCGCCCTGGCAGAACAGGGGGAAGTTGTGTTGGGGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGGTGGCCCAGCGTATGGGCAAACGCGCGTTTGTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGAGTGACTGCGGGCGCATCGGCTCC‑GGATAGTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGTTGGGCggtggt < 2:100858/251‑1 (MQ=255) ggTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGcagcag > 1:402646/1‑235 (MQ=255) ggTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACGCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCTAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTTCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGcagcag < 2:402646/235‑1 (MQ=255) cgcCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTGCAACTCCAACCGTCTGGCGGAGCTGGCACAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGACAGATATCCAGGAAGCGGGGGTGACAGAGGTTAAATGCGTAGGCGAGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGGGGCGGTGAAGCCATTc > 1:427803/1‑251 (MQ=255) ccTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGATTGCAGCAGCTGGGCGGGGGTGAAGCCATTCCGc > 1:497459/1‑251 (MQ=255) tGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTg > 1:83010/1‑251 (MQ=255) ggCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCt < 1:576564/249‑1 (MQ=255) aaCAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAACACTCCTCCAACTCCAACCGACTGTAGGAGCTGGCCCAGCGTATGGGCACAAGCGCGTTTTTG‑ATTGACGATGCGAAAGATATACAGGAAGAGTGGGAGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGGATCGGCACC‑GGATATTCTGGAGCAAAATGAGGTAGAGAGTTTGAAGCAGCTGGGCGGTGGTGAAGACATTCCGCTggcag > 2:334679/1‑246 (MQ=255) ggAAGTTTTGTGGGTGGTCGGTTCGAAAATCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGTTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTgaag < 2:555848/251‑1 (MQ=255) aaGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCACCTACCACCGTCGGGCTGAGCTGTCACAGCGTATGGGAACACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGACGATTGGGTGAAATAGGCTAAATGCGTTGTCGTGACTGCGGGCGCCTCGGCTCC‑GGATATTCTGGTGCAAAATGTTGTGGCAAGCTTGCAGTCGCTGGGCGGTGGTGAAGTCCTTCCGCTGGAAGGCCGTgtagaa < 1:413056/251‑1 (MQ=255) gtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACAtt < 1:61434/250‑1 (MQ=255) gtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACAtt < 1:465199/250‑1 (MQ=255) cGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAg < 2:571500/251‑1 (MQ=255) gggtCGAAAAACGCCTCCAACCCCAACCTGCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGTTTAAATGCGTCTGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTTGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTTAAGCTATGCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGt < 2:89839/248‑1 (MQ=255) ggTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGt < 2:524423/251‑1 (MQ=255) gTTCGAAAAACTCCTCCGACTCCAGCCGGCTGGCGTAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCTGCGTGACTGCGTGCGCATCGGCTCC‑GGATTTTCTGGTGCAAAATGTGGTGGCACGGTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTg < 1:436359/251‑1 (MQ=255) gTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCTGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGTTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTg < 1:423636/251‑1 (MQ=255) gTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGAGCAAACGCGCGTTTTTG‑ATTGACGATGCGAGAGATATCCAGGAAGAGTGGTTGAAAGAGGTTAAATGCGTCGGCGGGACTGCGGGCGCATCGGCTCC‑GGCTATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGGGACGCCATTCCGCTGGAAGGCCTTGGAGAAAACGTTGTTTTCGAAGTg > 1:524375/1‑251 (MQ=255) aaaaCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGaaa < 1:239648/251‑1 (MQ=255) aCTCCTCCACCTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAagag < 1:137683/251‑1 (MQ=255) aCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAagag < 1:204957/251‑1 (MQ=255) tccaactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCg > 1:386927/1‑251 (MQ=255) ccaactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCgt < 2:45236/251‑1 (MQ=255) aactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCgtgt < 1:452402/251‑1 (MQ=255) actccaacTGTCGGGCGGTGTGGGTCCAGCGTATGGGTAATCGCGTGTTTGTG‑ATTGACGAGGCGAAAGATATCCTTGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCTGGACTGCTGGCTCATCGGTGCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGATGGCCGTGCAGAAAACATCGTTTTCGACGTGCCGAAAGAGC‑TGTGTGTc < 2:22774/251‑1 (MQ=255) actccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCTCGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTc < 1:680059/251‑1 (MQ=255) actccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTGTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTc < 1:283442/251‑1 (MQ=255) tccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTgg < 1:478744/142‑1 (MQ=255) tacaacCGTCTGGCGGCGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCA‑GGATATTCTgg > 2:478744/3‑142 (MQ=255) caacCGTCTGGCGGAGCTGGCCCAGCGTACGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGATGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGGGGTGGCACTTTTGCAGCAGCTGGTCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGata < 2:36942/251‑1 (MQ=255) ccGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTc < 1:445459/251‑1 (MQ=255) cGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCg < 1:294965/251‑1 (MQ=255) tGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGCTATTCGTGa > 2:325623/1‑250 (MQ=255) ggCGGAGCTGGCCCAGCGTATGGGCAATCGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAg < 1:433171/251‑1 (MQ=255) ggCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTg < 1:70327/248‑1 (MQ=255) ggCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAg < 1:588877/251‑1 (MQ=255) cccAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTc < 1:325623/251‑1 (MQ=255) gCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTa < 1:561390/251‑1 (MQ=255) gTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGACGAGTGGGTGAAAGAGGTTACATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGACGTGCAGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagc > 1:324369/1‑251 (MQ=255) aTGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTg > 1:139027/1‑135 (MQ=255) aTGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTg < 2:139027/135‑1 (MQ=255) aTGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcag < 1:30656/251‑1 (MQ=255) aTGGGCAAACGCGCGTTTTTG‑ATTGACGAAGCGAAAGATATCCAGGAAGAGTGGGAGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcag < 2:260109/251‑1 (MQ=255) tGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcagc > 1:118765/1‑251 (MQ=255) gCAAAAGCGCGATTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGCGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAACATGAGGTGGAACGCTTGCAGCAGCTGGGCGGTGGTGAAGACATTACGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCGTG‑GCGTCGATATTCGTGAAGCTGCTTGCGTCATTAGCAGCCt > 2:133497/1‑250 (MQ=255) cAAACGCGCGTTGTTG‑AATGATGATGCGAAAGATATCCAGGAAGAGTGGGGTAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTTGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTaa < 1:445615/251‑1 (MQ=255) aaCGCGCGTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGt < 1:633690/251‑1 (MQ=255) cgcgcgTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGCGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGTGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTTAAGAAAACATTGTTTTCGACGTGCCGGTAGAGC‑TGCGTGTCGATATTCGTGACGTCGATTAAGGCATTAGCCGCCTAAGtt > 2:126214/1‑250 (MQ=255) gcgTTTTTG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGAGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGAGTCGATCTTCGTGACGTCGATTAAGACATTAGCAGCCTAAGTTATGc > 1:618280/1‑251 (MQ=255) gcgTTTTAG‑ATTGCCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCCGGCGCATCGGCTCC‑GGATATTCTGGTGCAAACTGTGGTGGCGCGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGACGTGAAGAAAACATTGTTTTCGAAGTGACGAAAGAGC‑TGCGTGTCGATATTCGTGTCGTCGATTCAGTCATTAGGCGCCTACGTTATGc > 2:85147/1‑251 (MQ=255) ttttG‑ATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGaa > 1:415356/1‑250 (MQ=255) ttg‑attgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGaaaa < 1:309022/250‑1 (MQ=255) gatggaCGATGCGAAAGATATCCAGGAAGAGGGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCACCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAATCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATg < 2:361665/246‑1 (MQ=255) attgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGcc > 1:533009/1‑251 (MQ=255) ttgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCg > 1:429558/1‑251 (MQ=255) cGATTCGAAAGATGTCCAGGAAGAGTGGGTGAAAGAGGTTAATTGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGCTCTTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATGCCGCTGGAATGTCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCACTAGCAGCCTAAGTTATGCGAAAATGCCGGTCt < 2:328650/251‑1 (MQ=255) gATGCTAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGGGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAAGGTGGTGGCACGGTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCtt < 1:635216/251‑1 (MQ=255) gAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTCGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAc > 2:180762/1‑251 (MQ=255) | CGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTG‑ATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCC‑GGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGC‑TGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAC > NC_000913/26779‑27270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |