| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 26,624 | T→G | S116S (TCT→TCG) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 26,624 | 0 | T | G | 98.4% | 196.2 / ‑5.1 | 64 | S116S (TCT→TCG) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (28/35); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
ACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑TC‑GCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTG > NC_000913/26386‑26866 | aCGAAGTGGTACATAACCGCTATGTGGGCGATAGGTTGCGTGAGCGAGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGACTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCCTATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCAtaggc > 1:272532/1‑247 (MQ=255) ccGCTATGAGGACGATAGCTCGCGTGAGCGTGGGGCTATCTTTCTTGAGCAGATTAGCGAAGTACAGGACGGCGCGATCCTGATTGTCTTCGCACACGGGGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGACGCGATTTGACGGAGGTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGGTGCGAAAAGTCGATTCTCATCGGCCACGCCGGGCATCCgg > 2:137683/1‑251 (MQ=255) attgggTCGATAGCTTTCGTGAGCGTGGGGTTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTGCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑TC‑GCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCCCCCGGAAg < 2:479571/245‑1 (MQ=255) gtgGTCGAGATCTTGCTTTAGCGTGGGGCTATCTTTATTGAGCAGATTATCGAAGTTCCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTTCGTAACGAAGCAAAAAGCCTCGATTTGACGGTGTGTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAATTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGa < 2:541023/251‑1 (MQ=255) aTAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGCAGTACCGGCCGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGCGACCAAAGGGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGCGGTGGAAggtacg > 1:651350/1‑247 (MQ=255) aGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAggtacgat < 1:504640/251‑7 (MQ=255) ggAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagt < 1:290244/250‑1 (MQ=255) ggCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtac < 1:1879/250‑1 (MQ=255) ggggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtacagta > 1:96062/1‑250 (MQ=255) gggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAAc > 1:58327/1‑251 (MQ=255) ggCTATCTTTATTGGGCAGATTAGCGAAGTACCGGCCGGCGCGATCCTGATTTTCCCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGGCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAAcc < 2:43184/251‑1 (MQ=255) gCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACTGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGt < 1:51101/251‑1 (MQ=255) gATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATc < 1:233382/251‑1 (MQ=255) tttAGAGAAGAACCGGACGGCGCGATCCTGCTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAAAGAAGCAAAAAGCCGCGATTTGACGGTGTTTGCTGCCACCTGTCCGCTGGTGACCAACGTGAATCTGGAAGTAGCACGCGCCAG‑CC‑GTCGTGGCGGAGAGTCGCTTCTCATAGGCCACGCCGGGCATCCGGAAGTGGAAGGTACAATGGGCCAGCACAGTAACCCGGCAggggggctgattct > 2:239633/2‑242 (MQ=255) cGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCg < 1:204726/251‑1 (MQ=255) gTACCGGACGGCGCGATCCTTATTTTCTCCGCACACGGTGTTTCGCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATGTGACGGTGTTTGATGCCCCCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATc < 2:194581/251‑1 (MQ=255) gaCCGGACGGCGAGATTCTGTTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTTGCGTTGTGGCGGTGTTTGATGCCACCGGTCCGCTGGGGTCCAAAGGGCATATGGAAGCCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCAGCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTCCAGTAACCCGGAAGGGGGAATGTATCTGGTAGaagcg < 2:376735/250‑4 (MQ=255) aCCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCg < 1:17025/250‑1 (MQ=255) cGGACAGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGACGAATCGCCg > 1:489675/1‑251 (MQ=255) gACGGCGCGATCCGTATTTCTTCCGCACCCGGTGTTTCGCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGAGTCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCTCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCAGGCCGGGCATCCGGAAGTGGAAGGTACGATGTGCCAGTACAGTAGCCCGGAAGGGGGAATTTATCTGGTCGAATTGCCGGa < 2:640303/251‑1 (MQ=255) aCGGCGCGATCCTGATTTTCTCCGCTCACGTTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGGTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGAc < 1:524642/251‑1 (MQ=255) aCGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGAc > 1:511134/1‑251 (MQ=255) cgcgATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtg < 1:618366/251‑1 (MQ=255) gcgATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtgt > 1:194208/1‑251 (MQ=255) gATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGCAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGGATGTATCTGGTCGAATCGCCGGACGAtgtgt > 1:567684/1‑251 (MQ=255) atttctgccGCACACGGTGTTTCTCAGGCGGTCCGTAACGAAGCAAAAAGCCGAGATTTGACGGTGTTTGATGCCCCCTGTCCGCTGGTGACCAAAGTGAATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGGGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGGATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAAca < 2:33752/244‑2 (MQ=255) atttatgccgactaggttgttCTTAGGCGGGACGTAAAGAAGCAAAAAGCAGCGATTTGACGGGGTTTTATGCAACCGGCCTGCTGGTGCCCAAAGTGCATATGGAAGGCGCCCGCGCCAGCTT‑GCAG‑GGCGAAGAGTCGATTCCCAACGGACACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCTGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGTGTGGAAACTg < 2:126036/232‑1 (MQ=255) tttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCTCCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTg < 1:16671/250‑1 (MQ=255) cGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTc > 1:589189/1‑250 (MQ=255) cGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCa < 1:324194/251‑1 (MQ=255) cacaCGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGTAGTGGTAGGTACGATGGGCCAGTACAGTAACCCGGGAGGGGGAATGTATCTGGTCGAATCGCCGGACGAGTTGGGGGAACTAACGGTCaa > 2:253061/1‑250 (MQ=255) acGGTGGTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGAGTTGACGGTGTGTGAGGCCCCCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAAc < 2:58327/251‑1 (MQ=255) tgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGa > 1:78523/1‑249 (MQ=255) aGGCGGTACGTAACGAAGCAAAAAGACGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGACGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGACGAGAAGctc > 1:483803/1‑251 (MQ=255) ggCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAATTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGctct < 1:382809/251‑1 (MQ=255) gtgtatgTAACCAAGCAAAAAGCCGTGAGTTGAAGTTGTTTGAGGCACCCTGTCCGCTGGTGCCCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGTAGTTACGATGGGCCAGTACAGGAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGGGTGGAAACTGACGGTCAAAATCGAAGAGAAGctctac < 2:479171/245‑3 (MQ=255) accgaaCGAAGCAAAAAGCCGTGATTTGACGGTTTTTGATGCCGCCTTGCCGCTGGTGTCCAAATTGCATTTGGAAGTCGCCCGCGCCAG‑CC‑GTCGGGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCttt < 2:430311/246‑1 (MQ=255) aCGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTa < 1:146492/251‑1 (MQ=255) aaCGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGAc > 1:397084/1‑251 (MQ=255) gAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCat > 1:392346/1‑125 (MQ=255) gAAGCAAAAAGCCGCGATTTGACGGGGTTTGATGCCACCTTTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCTAAGAGTCGATTCTCATCGGCCACGCCGGGCat < 2:392346/126‑2 (MQ=255) aaaaGCCGCGATTGGACTGTGTTTGTTGCTACCTGGCCGCTGGTGACCAAAGTGCTTATGGAAGTCGCCCGCGCCAG‑CC‑GGCGTGGCGAAGAGGCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTATCCCGGAAGGGGGAATGTATCTGGTAGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCAc < 2:356409/251‑1 (MQ=255) aaaGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCAACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGAcaa > 1:389067/1‑247 (MQ=255) gATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTc < 1:103076/248‑1 (MQ=255) gATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCCCTCCTTTCTGACCCAGACCAcactttc > 2:103076/1‑242 (MQ=255) gACGGTGCTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGACGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGCAGGTACGATGGGCCAGTACCGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCAGGACGATGCGGGGAAACTGACGGTCAAAAACGAAGAAAAGCTCTCCTTTATGACCCAGACCACGCTTTCGGTGGATg > 2:252639/1‑251 (MQ=255) cGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGac > 1:645707/1‑251 (MQ=255) gtgtTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGaa < 2:158486/144‑1 (MQ=255) gtgtTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGaa > 1:158486/1‑144 (MQ=255) ttaTGCCCCCTGTCCGGTGGTGACCAAATTGATTATGTAAGCCGCCCCCGCC‑G‑TCCGTCGTGGCGAAGAGCCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAATGTGCGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGTCGATGTGGGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACTCGTCt < 2:122761/248‑1 (MQ=255) gATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGa < 1:480145/251‑1 (MQ=255) gCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAag > 1:126007/1‑185 (MQ=255) gCTGGTGACCAAAGTGAATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGACACGCCGGGCATCCGGACGTGGAAGGAACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAAAGAAGAGAAGCTCTCCTTTATGAACCAGACCACGCTGTCGGTGGAAGACACGtaggttgtgaacgccgggc > 1:669822/1‑233 (MQ=255) aaGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGCAGTGGAAGGTACGCTGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGGGTGGAAACTGACGGTCAAAAACGAAGAGACGCTCTCCTTTTTGACCCAGAACACGCTGTCGGTGGATGACACGTCTGATGTGATAGACGCGCTGCGGAAACGc > 2:257378/1‑251 (MQ=255) aGTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCt < 1:38673/251‑1 (MQ=255) gTGCATATGGAAGTCGCCCGCGCCAG‑CC‑GTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGGTGGGCCAGTACAGGAACCCGGAAGGGGGAATGGATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCATTTATGAACCAGACCACGCGATCGGAGGGTGACCCTTCTGGTGTGATCGAAGGGCGGCGTAGACGCtt > 2:465199/1‑251 (MQ=255) ggAAGTCGGCCCCGCCAG‑CC‑GTCGGTGCGGAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGTTGGGGCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGTGTTGAAACTGACGGTCAAAAACGAAGAGAATCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGaaaa < 2:558736/251‑1 (MQ=255) cagccgtcGTGGCGAAGCGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGAACGATGGGCCAGTACAGTCAACCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCAAGCAGTCGGTGGATGACACGTCTGATGTGATCGCAGCACTGCGTTAACGCTTCCCGAAACTTGTCGGACCgcgc > 2:576564/8‑250 (MQ=255) cagccgtcGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCgcgc > 1:546223/8‑250 (MQ=255) ggccgtcggggCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGGGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCaa < 2:546223/242‑1 (MQ=255) cgtcGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGCGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGAAACGTCTGATGGGATCGACGCGCTGCGTAAACGCTTCCCGCAAATTGTAGGTCCGCGCaa > 2:337601/4‑248 (MQ=255) cGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGGCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCGTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATg < 2:397084/250‑1 (MQ=255) gggCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGTAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACa < 2:496297/250‑1 (MQ=255) gaagagTCGATTCTCAGCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCGGATGTGATCGACGCGCTGCGTAAACGCTTGCCGAAAATTGTCGGTCCTCGCAATGATGGCATCTg < 2:287409/251‑1 (MQ=255) | ACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAG‑TC‑GCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTG > NC_000913/26386‑26866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |