Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,685	G→A	R160K (AGA→AAA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,685	0	G	A	100.0%	146.0 / NA	47	R160K (AGA→AAA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/20);  total (27/20)

GGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAAC  >  NC_000913/25438‑25918                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGc                                                                                                                                                                                                                                        >  1:537987/1‑251 (MQ=255)  gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTATACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCCACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGGTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACATTGCCGGTACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTTGAAGGTTTTTTACCTTCTAAAGCg                                                                                                                                                                                                                                       >  2:73471/1‑251 (MQ=255)    aTTAGCGTGATCCTGGCATTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑GGGGCACCCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaa                                                                                                                                                                                                                                     <  1:16846/251‑1 (MQ=255)      tAGCGTGATCCTGGCAGTGATGATGTAGCGCTCGAAGGCCACGCAGAAGCTAAACAATTTCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGAACCCTGTGC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaaa                                                                                                                                                                                                                                    <  1:337975/250‑1 (MQ=255)       aGCGTGACCCTGGCAGTGATGATGTAACGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGAGCGC‑TGGGCAACCTGTTA‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCCACTTCCACCTTGCAGATACTGCCATCTGAGTCGGTGCGGCACTGATTGTGCTGGAAAGTTTTTTACCTTCTAAAGCGaaaa                                                                                                                                                                                                                                   >  2:434273/1‑250 (MQ=255)                cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGTCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCCACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCCCTGATTGTGCTGGAAGGTTTTTTCACTTCTAAAGCGAAAAAACattaat                                                                                                                                                                                                                          >  1:121143/1‑250 (MQ=255)                   gCAGTGATGATGTATCGCTCAAGGGCCCCGCAGAAGCTAAACAATATCGCTTACGCGCTGCTTATTGACGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGCTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCAGCCCTTGTTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGCAAAAACAAAAATAAAc                                                                                                                                                                                                                      >  2:247943/1‑251 (MQ=255)                           gatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCgg                                                                                                                                                                                                              >  1:503238/1‑251 (MQ=255)                                       gAAGGCCACGCAGAAGCTACACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGGGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGACGGTTATTTACCTTCTAAAGCGAAAAAACAAAAATAAACCCTGACGgctgcgcagctg                                                                                                                                                                                                   >  2:44223/1‑244 (MQ=255)                                           gCCACGCAGAAGCAAAACAATACCCCTTACGCGTTGATTAGTGGGGGCGCGT‑GGGGCACCCTGTTG‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCa                                                                                                                                                                                              <  1:534247/251‑1 (MQ=255)                                               cGCAGAGGCTAAAAATTATCGCTTCCGCGTTGTTTATTGGCGGCGCGC‑TGGGCACCCTTTCC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                          <  1:23225/251‑1 (MQ=255)                                                    aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCg                                                                                                                                                                                     >  1:675384/1‑251 (MQ=255)                                                     aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTA‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGCCGCATCTTATCCgg                                                                                                                                                                                    >  1:428399/1‑251 (MQ=255)                                                       cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATCTGATCGACTTCTAAGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTTCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACCATAATAAACCCTGCCGGATCCTATGATGACGCATCTTATACGGcc                                                                                                                                                                                  >  2:603823/1‑251 (MQ=255)                                                         aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTTTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                                                                                                 >  2:62174/1‑250 (MQ=255)                                                         aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                                                                                                 >  1:297454/1‑250 (MQ=255)                                                                  cgtttACGCGTTTATTATTGGCGGCTGGT‑TGGGCA‑CCCGTTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAAGAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgc                                                                                                                                                                        <  1:589289/247‑1 (MQ=255)                                                                            cTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGTCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGTTGCGATGCTGACGCCTCTTAGCCTGCCTACAGATTGCTGCGAAATctt                                                                                                                                                             >  2:459028/1‑249 (MQ=255)                                                                                tttttGGCGG‑GCGCCTGGGCACCCCGTCC‑GGCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAgg                                                                                                                                                          <  2:136027/247‑1 (MQ=255)                                                                                   tgggcggcGCGC‑TGGGCACCCGTTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCg                                                                                                                                                       <  1:298296/248‑1 (MQ=255)                                                                                           gcgc‑TGGGCAACCTGTTC‑GCCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCg                                                                                                                                              <  1:90968/251‑1 (MQ=255)                                                                                                 gggCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGttt                                                                                                                                           <  1:164774/249‑1 (MQ=255)                                                                                                        cTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                                                                  >  1:9640/1‑251 (MQ=255)                                                                                                          gTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                <  1:109661/251‑1 (MQ=255)                                                                                                          gTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCGTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAATCCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                 <  1:645754/250‑1 (MQ=255)                                                                                                           gttggtccgacTGTGTCACGTCGTCGTGGTCGATATGTACGACTTTTACGTCGGCGGCTGGCACGTCGCTACCTGCAACCTTGACGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATTCGGCCTACAGATGGCTGCGAAATCGTAGGCCGGATAAGTCGTTTACGCCGCATcc                                                                                                                                <  2:327675/241‑1 (MQ=255)                                                                                                             c‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATCCTGCCATCTGTGGCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGTTGCGATGCTGACGCCTCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATCAGGCGTTTACGCCGCCTCCGGc                                                                                                                             >  2:264680/1‑251 (MQ=255)                                                                                                                 ccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGATGGCACTTCGCCACCTTCAACCTTGCCGATAATGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGACGATTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGACGGATGCGATGCTGCCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCaa                                                                                                                           >  2:82627/1‑250 (MQ=255)                                                                                                                            aCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTACCATCTGTGTCGATGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAat                                                                                                               >  1:641582/1‑251 (MQ=255)                                                                                                                              ggtttCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTAGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                             <  2:159955/248‑1 (MQ=255)                                                                                                                                  tCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGAGTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAag                                                                                                          <  2:518162/250‑1 (MQ=255)                                                                                                                                  tCGTTGTCGATATGATCGACTTCTACGGCGGCGGCTGGCACTACGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAAGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaa                                                                                                           <  2:69860/249‑1 (MQ=255)                                                                                                                                         cGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAc                                                                                                   <  1:62174/250‑1 (MQ=255)                                                                                                                                                  cGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                     >  2:133823/1‑207 (MQ=255)                                                                                                                                                  cGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                     <  1:133823/207‑1 (MQ=255)                                                                                                                                                     cTTCTACGTCGGCGGATGGCACTTCGCCACCTTCAACCTTGCCGCTACTGCCATCTGTGTCGGTGCGGCACTGATTGTTCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAAAAATAAACACTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCGGGATAAGGCGTTTCCGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCAAGTCTGa                                                                                       >  2:562895/1‑250 (MQ=255)                                                                                                                                                      ttCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt                                                                                     >  1:460199/1‑251 (MQ=255)                                                                                                                                                            gTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc                                                                               >  1:534201/1‑251 (MQ=255)                                                                                                                                                                        cACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAgcg                                                                   <  1:561026/251‑1 (MQ=255)                                                                                                                                                                                                         aTCTGAGTAGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTAACTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGAAGCATTTTATAAGGCCTACAGATTGCAGCGAAATCGTAGGACGGCTAAGGCGTTTACGCGGCCACAGGCAGAAATCCTTAAATGTAAGAGCAAAAGTGAATGTCTAAATCTGTACAGAGCAATAGAGCCGTAATGGTGCACTTCACACTAAAACtagtcg                                  >  1:680633/1‑246 (MQ=255)                                                                                                                                                                                                                                   gCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGc        >  1:154019/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGGAAGGTTTTTTCCCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAAAGCAAACCTGAATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGt                                                         >  2:627298/1‑200 (MQ=255)                                                                                                                                                                                                                                     tGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGt                                                         <  1:627298/200‑1 (MQ=255)                                                                                                                                                                                                                                      ggAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaac     >  1:539197/1‑251 (MQ=255)                                                                                                                                                                                                                                         aGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTAAAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCGACCCGCaacaac  >  2:110608/1‑251 (MQ=255)                                                                                                                                                                                                                                                   ccTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCTGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc                                                                                                  <  2:518443/145‑1 (MQ=255)                                                                                                                                                                                                                                                   ccTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc                                                                                                  >  1:518443/1‑145 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          GGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAAC  >  NC_000913/25438‑25918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑