Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,677	G→A	L157L (TTG→TTA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,677	0	G	A	95.7%	138.2 / NA	47	L157L (TTG→TTA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/19);  minor base C (2/0);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAAC  >  NC_000913/25431‑25918                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  tGCGATTGGTATTAGAGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGACACTGCACTCTGGGTAGGTGAGGCCATGTTTGTGCTTGAAGGTTTTTTACCtt                                                                                                                                                                                                                                               >  2:232081/1‑251 (MQ=255)      ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaa                                                                                                                                                                                                                                           >  2:164774/1‑249 (MQ=255)        ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGc                                                                                                                                                                                                                                        >  1:537987/1‑251 (MQ=255)         gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTATACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCCACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGGTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACATTGCCGGTACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTTGAAGGTTTTTTACCTTCTAAAGCg                                                                                                                                                                                                                                       >  2:73471/1‑251 (MQ=255)           aTTAGCGTGATCCTGGCATTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑GGGGCACCCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaa                                                                                                                                                                                                                                     <  1:16846/251‑1 (MQ=255)             tAGCGTGATCCTGGCAGTGATGATGTAGCGCTCGAAGGCCACGCAGAAGCTAAACAATTTCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGAACCCTGTGC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaaa                                                                                                                                                                                                                                    <  1:337975/250‑1 (MQ=255)              aGCGTGACCCTGGCAGTGATGATGTAACGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGAGCGC‑TGGGCAACCTGTTA‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCCACTTCCACCTTGCAGATACTGCCATCTGAGTCGGTGCGGCACTGATTGTGCTGGAAAGTTTTTTACCTTCTAAAGCGaaaa                                                                                                                                                                                                                                   >  2:434273/1‑250 (MQ=255)                       cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGTCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCCACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCCCTGATTGTGCTGGAAGGTTTTTTCACTTCTAAAGCGAAAAAACattaat                                                                                                                                                                                                                          >  1:121143/1‑250 (MQ=255)                          gCAGTGATGATGTATCGCTCAAGGGCCCCGCAGAAGCTAAACAATATCGCTTACGCGCTGCTTATTGACGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGCTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCAGCCCTTGTTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGCAAAAACAAAAATAAAc                                                                                                                                                                                                                      >  2:247943/1‑251 (MQ=255)                                  gatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCgg                                                                                                                                                                                                              >  1:503238/1‑251 (MQ=255)                                              gAAGGCCACGCAGAAGCTACACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGGGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGACGGTTATTTACCTTCTAAAGCGAAAAAACAAAAATAAACCCTGACGgctgcgcagctg                                                                                                                                                                                                   >  2:44223/1‑244 (MQ=255)                                                  gCCACGCAGAAGCAAAACAATACCCCTTACGCGTTGATTAGTGGGGGCGCGT‑GGGGCACCCTGTTG‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCa                                                                                                                                                                                              <  1:534247/251‑1 (MQ=255)                                                      cGCAGAGGCTAAAAATTATCGCTTCCGCGTTGTTTATTGGCGGCGCGC‑TGGGCACCCTTTCC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                          <  1:23225/251‑1 (MQ=255)                                                           aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCg                                                                                                                                                                                     >  1:675384/1‑251 (MQ=255)                                                            aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTA‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGCCGCATCTTATCCgg                                                                                                                                                                                    >  1:428399/1‑251 (MQ=255)                                                              cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATCTGATCGACTTCTAAGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTTCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACCATAATAAACCCTGCCGGATCCTATGATGACGCATCTTATACGGcc                                                                                                                                                                                  >  2:603823/1‑251 (MQ=255)                                                                aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTTTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                                                                                                 >  2:62174/1‑250 (MQ=255)                                                                aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                                                                                                 >  1:297454/1‑250 (MQ=255)                                                                         cgtttACGCGTTTATTATTGGCGGCTGGT‑TGGGCA‑CCCGTTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAAGAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgc                                                                                                                                                                        <  1:589289/247‑1 (MQ=255)                                                                                   cTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGTCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGTTGCGATGCTGACGCCTCTTAGCCTGCCTACAGATTGCTGCGAAATctt                                                                                                                                                             >  2:459028/1‑249 (MQ=255)                                                                                       tttttGGCGG‑GCGCCTGGGCACCCCGTCC‑GGCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAgg                                                                                                                                                          <  2:136027/247‑1 (MQ=255)                                                                                          tgggcggcGCGC‑TGGGCACCCGTTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCg                                                                                                                                                       <  1:298296/248‑1 (MQ=255)                                                                                                  gcgc‑TGGGCAACCTGTTC‑GCCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCg                                                                                                                                              <  1:90968/251‑1 (MQ=255)                                                                                                        gggCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGttt                                                                                                                                           <  1:164774/249‑1 (MQ=255)                                                                                                               cTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                                                                  >  1:9640/1‑251 (MQ=255)                                                                                                                 gTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                <  1:109661/251‑1 (MQ=255)                                                                                                                 gTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCGTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAATCCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                 <  1:645754/250‑1 (MQ=255)                                                                                                                  gttggtccgacTGTGTCACGTCGTCGTGGTCGATATGTACGACTTTTACGTCGGCGGCTGGCACGTCGCTACCTGCAACCTTGACGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATTCGGCCTACAGATGGCTGCGAAATCGTAGGCCGGATAAGTCGTTTACGCCGCATcc                                                                                                                                <  2:327675/241‑1 (MQ=255)                                                                                                                    c‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATCCTGCCATCTGTGGCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGTTGCGATGCTGACGCCTCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATCAGGCGTTTACGCCGCCTCCGGc                                                                                                                             >  2:264680/1‑251 (MQ=255)                                                                                                                        ccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGATGGCACTTCGCCACCTTCAACCTTGCCGATAATGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGACGATTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGACGGATGCGATGCTGCCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCaa                                                                                                                           >  2:82627/1‑250 (MQ=255)                                                                                                                                   aCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTACCATCTGTGTCGATGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAat                                                                                                               >  1:641582/1‑251 (MQ=255)                                                                                                                                     ggtttCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTAGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                             <  2:159955/248‑1 (MQ=255)                                                                                                                                         tCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGAGTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAag                                                                                                          <  2:518162/250‑1 (MQ=255)                                                                                                                                         tCGTTGTCGATATGATCGACTTCTACGGCGGCGGCTGGCACTACGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAAGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaa                                                                                                           <  2:69860/249‑1 (MQ=255)                                                                                                                                                cGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAc                                                                                                   <  1:62174/250‑1 (MQ=255)                                                                                                                                                         cGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                     >  2:133823/1‑207 (MQ=255)                                                                                                                                                         cGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                     <  1:133823/207‑1 (MQ=255)                                                                                                                                                            cTTCTACGTCGGCGGATGGCACTTCGCCACCTTCAACCTTGCCGCTACTGCCATCTGTGTCGGTGCGGCACTGATTGTTCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAAAAATAAACACTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCGGGATAAGGCGTTTCCGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCAAGTCTGa                                                                                       >  2:562895/1‑250 (MQ=255)                                                                                                                                                             ttCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt                                                                                     >  1:460199/1‑251 (MQ=255)                                                                                                                                                                   gTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc                                                                               >  1:534201/1‑251 (MQ=255)                                                                                                                                                                               cACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAgcg                                                                   <  1:561026/251‑1 (MQ=255)                                                                                                                                                                                                                aTCTGAGTAGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTAACTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGAAGCATTTTATAAGGCCTACAGATTGCAGCGAAATCGTAGGACGGCTAAGGCGTTTACGCGGCCACAGGCAGAAATCCTTAAATGTAAGAGCAAAAGTGAATGTCTAAATCTGTACAGAGCAATAGAGCCGTAATGGTGCACTTCACACTAAAACtagtcg                                  >  1:680633/1‑246 (MQ=255)                                                                                                                                                                                                                                          gCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGc        >  1:154019/1‑251 (MQ=255)                                                                                                                                                                                                                                            tGGAAGGTTTTTTCCCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAAAGCAAACCTGAATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGt                                                         >  2:627298/1‑200 (MQ=255)                                                                                                                                                                                                                                            tGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGt                                                         <  1:627298/200‑1 (MQ=255)                                                                                                                                                                                                                                             ggAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaac     >  1:539197/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTAAAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCGACCCGCaacaac  >  2:110608/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  TGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAAC  >  NC_000913/25431‑25918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑