Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,598	A→G	D131G (GAC→GGC)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,598	0	A	G	100.0%	150.1 / NA	48	D131G (GAC→GGC)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/21);  total (27/21)

ATTATGCGCGTAACTATGGCGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTT‑CTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAC  >  NC_000913/25352‑25841                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    aTTATGCGCGTAACTATGGCGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTT‑CTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTgg                                                                                                                                                                                                                                                 >  1:136027/1‑251 (MQ=255)           tAACTATGGCGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTT‑CTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAAACTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGcc                                                                                                                                                                                                                                        >  1:121942/1‑250 (MQ=255)                    cgcgGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTT‑CTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAAcc                                                                                                                                                                                                                              >  1:69860/1‑251 (MQ=255)                               gttcctttGCCGTTAGCGGCGGCGGCCAGCGTTGTTT‑CTTTGCCGGTATTGCGTTTGTTATGAGGGTGATCCGGGCAGTGAGG‑ATGTATCGCCCGAAGGCAAGCCAGAGGCTAAACAATATCG‑TTACCGCGCGGTTTATTGGCGGCGCGG‑TGGGCACCTTTTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACt                                                                                                                                                                                                                   <  1:309758/245‑1 (MQ=255)                                           atagggtcggcTGGCAGC‑TTGGTTTCTTCGCCGTTATTGCGTTTGTGATGAGGGTAATTCTGGCATTG‑TGTATGTACCGCTCGAAGTCCACGAAGACGCAAAACAATATCGCTTCC‑GCTCTTATTATTGGCGGCGCGC‑TGGGAATCCTTTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTc                                                                                                                                                                                                       <  1:508166/244‑1 (MQ=255)                                             gtcggcggCTGGCGGTGTGGGTT‑CTTGGCCGGTATTGCGATTGGTATTCGCGTGTGCTTGCCAGTGATG‑ATGTCTCCCTCGAAGGCCCCGCAGACGCTAAACATTATCGTTTCC‑TCGTTGATTATGGTCGCCGCGC‑TGGGCAACCTTTCC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCgg                                                                                                                                                                                                     <  1:104397/249‑1 (MQ=255)                                                 gcggcTGGCAGCGTTGGTT‑TTTTGCTGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTACCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATGGGCGGCGCGC‑TGGGCACCCTGTCC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCg                                                                                                                                                                                                 <  1:646587/251‑1 (MQ=255)                                                                           cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTAGTTGTCGATATGATCGACTTCTACGCCGGCGGCTGGCCCTTCGCCCCCTTCACCCTTGCCGATCCAGCCATCTGTGGCGGGTCGGCACTAACTGTGCTGGAAGGttttt                                                                                                                                                                        >  2:534247/1‑251 (MQ=255)                                                                                 tGCGATTGGTATTAGAGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGACACTGCACTCTGGGTAGGTGAGGCCATGTTTGTGCTTGAAGGTTTTTTACCtt                                                                                                                                                                  >  2:232081/1‑251 (MQ=255)                                                                                      ttGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaa                                                                                                                                                              >  2:164774/1‑249 (MQ=255)                                                                                        ggTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGc                                                                                                                                                           >  1:537987/1‑251 (MQ=255)                                                                                         gTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTATACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCCACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGGTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACATTGCCGGTACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTTGAAGGTTTTTTACCTTCTAAAGCg                                                                                                                                                          >  2:73471/1‑251 (MQ=255)                                                                                           aTTAGCGTGATCCTGGCATTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑GGGGCACCCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaa                                                                                                                                                        <  1:16846/251‑1 (MQ=255)                                                                                             tAGCGTGATCCTGGCAGTGATG‑ATGTAGCGCTCGAAGGCCACGCAGAAGCTAAACAATTTCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGAACCCTGTGC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaaa                                                                                                                                                       <  1:337975/250‑1 (MQ=255)                                                                                              aGCGTGACCCTGGCAGTGATG‑ATGTAACGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGAGCGC‑TGGGCAACCTGTTA‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCCACTTCCACCTTGCAGATACTGCCATCTGAGTCGGTGCGGCACTGATTGTGCTGGAAAGTTTTTTACCTTCTAAAGCGaaaa                                                                                                                                                      >  2:434273/1‑250 (MQ=255)                                                                                                       cTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGTCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCCACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCCCTGATTGTGCTGGAAGGTTTTTTCACTTCTAAAGCGAAAAAACattaat                                                                                                                                             >  1:121143/1‑250 (MQ=255)                                                                                                          gCAGTGATG‑ATGTATCGCTCAAGGGCCCCGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGCTTATTGACGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGCTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCAGCCCTTGTTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGCAAAAACAAAAATAAAc                                                                                                                                         >  2:247943/1‑251 (MQ=255)                                                                                                                  g‑atgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCgg                                                                                                                                 >  1:503238/1‑251 (MQ=255)                                                                                                                               gAAGGCCACGCAGAAGCTACACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGGGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGACGGTTATTTACCTTCTAAAGCGAAAAAACAAAAATAAACCCTGACGgctgcgcagctg                                                                                                                      >  2:44223/1‑244 (MQ=255)                                                                                                                                   gCCACGCAGAAGCAAAACAATACCCCTTAC‑GCGTTGATTAGTGGGGGCGCGT‑GGGGCACCCTGTTG‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCa                                                                                                                 <  1:534247/251‑1 (MQ=255)                                                                                                                                       cGCAGAGGCTAAAAATTATCGCTTCC‑GCGTTGTTTATTGGCGGCGCGC‑TGGGCACCCTTTCC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                             <  1:23225/251‑1 (MQ=255)                                                                                                                                            aaGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCg                                                                                                        >  1:675384/1‑251 (MQ=255)                                                                                                                                             aGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTA‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGCCGCATCTTATCCgg                                                                                                       >  1:428399/1‑251 (MQ=255)                                                                                                                                               cTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATCTGATCGACTTCTAAGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTTCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACCATAATAAACCCTGCCGGATCCTATGATGACGCATCTTATACGGcc                                                                                                     >  2:603823/1‑251 (MQ=255)                                                                                                                                                 aaaCAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTTTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                    >  2:62174/1‑250 (MQ=255)                                                                                                                                                 aaaCAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                    >  1:297454/1‑250 (MQ=255)                                                                                                                                                          cgtttAC‑GCGTTTATTATTGGCGGCTGGT‑TGGGCA‑CCCGTTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAAGAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgc                                                                                           <  1:589289/247‑1 (MQ=255)                                                                                                                                                                     cTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGTCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGTTGCGATGCTGACGCCTCTTAGCCTGCCTACAGATTGCTGCGAAATctt                                                                                >  2:459028/1‑249 (MQ=255)                                                                                                                                                                         tttttGGCGG‑GCGCCTGGGCACCCCGTCC‑GGCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAgg                                                                             <  2:136027/247‑1 (MQ=255)                                                                                                                                                                            tgggcggcGCGC‑TGGGCACCCGTTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCg                                                                          <  1:298296/248‑1 (MQ=255)                                                                                                                                                                                    gcgc‑TGGGCAACCTGTTC‑GCCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCg                                                                 <  1:90968/251‑1 (MQ=255)                                                                                                                                                                                          gggCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGttt                                                              <  1:164774/249‑1 (MQ=255)                                                                                                                                                                                                 cTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                     >  1:9640/1‑251 (MQ=255)                                                                                                                                                                                                   gTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                   <  1:109661/251‑1 (MQ=255)                                                                                                                                                                                                   gTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCGTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAATCCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                    <  1:645754/250‑1 (MQ=255)                                                                                                                                                                                                    gttggtccgacTGTGTCACGTCGTCGTGGTCGATATGTACGACTTTTACGTCGGCGGCTGGCACGTCGCTACCTGCAACCTTGACGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATTCGGCCTACAGATGGCTGCGAAATCGTAGGCCGGATAAGTCGTTTACGCCGCATcc                                                   <  2:327675/241‑1 (MQ=255)                                                                                                                                                                                                      c‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATCCTGCCATCTGTGGCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGTTGCGATGCTGACGCCTCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATCAGGCGTTTACGCCGCCTCCGGc                                                >  2:264680/1‑251 (MQ=255)                                                                                                                                                                                                          ccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGATGGCACTTCGCCACCTTCAACCTTGCCGATAATGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGACGATTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGACGGATGCGATGCTGCCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCaa                                              >  2:82627/1‑250 (MQ=255)                                                                                                                                                                                                                     aCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTACCATCTGTGTCGATGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAat                                  >  1:641582/1‑251 (MQ=255)                                                                                                                                                                                                                       ggtttCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTAGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                <  2:159955/248‑1 (MQ=255)                                                                                                                                                                                                                           tCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGAGTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAag                             <  2:518162/250‑1 (MQ=255)                                                                                                                                                                                                                           tCGTTGTCGATATGATCGACTTCTACGGCGGCGGCTGGCACTACGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAAGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaa                              <  2:69860/249‑1 (MQ=255)                                                                                                                                                                                                                                  cGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAc                      <  1:62174/250‑1 (MQ=255)                                                                                                                                                                                                                                           cGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                        >  2:133823/1‑207 (MQ=255)                                                                                                                                                                                                                                           cGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                        <  1:133823/207‑1 (MQ=255)                                                                                                                                                                                                                                              cTTCTACGTCGGCGGATGGCACTTCGCCACCTTCAACCTTGCCGCTACTGCCATCTGTGTCGGTGCGGCACTGATTGTTCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAAAAATAAACACTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCGGGATAAGGCGTTTCCGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCAAGTCTGa          >  2:562895/1‑250 (MQ=255)                                                                                                                                                                                                                                               ttCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt        >  1:460199/1‑251 (MQ=255)                                                                                                                                                                                                                                                     gTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc  >  1:534201/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ATTATGCGCGTAACTATGGCGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTT‑CTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATG‑ATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAC‑GCGCTGATTATTGGCGGCGCGC‑TGGGCAACCTGTTC‑GACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAC  >  NC_000913/25352‑25841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑