Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 24,977 C→T L863L (CTG→TTG)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091324,9770CT96.0% 288.6 / 0.2 102L863L (CTG→TTG) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (40/56);  total (43/59)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 103 total aligned reads displayed.

GTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTCAATC  >  NC_000913/24735‑25217
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
ggtaaaaaTTCGTCTTCGCCTGTGAGT‑GGTACGCAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGAGGTGTTCTGGGACGTGATGTTGAAAGTGCGTGTCGTAGTGAACAAAGTCACTGAGCAAGCGCGTGCAGACCAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTTTCGTCGAAATTGACCGGGCTGGGCGATGAATTACGATTTGTCttgttgccc                                                                                                                                                                                                                                           <  1:541477/248‑4 (MQ=255)
  gAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTTGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTc                                                                                                                                                                                                                                         <  1:421707/251‑1 (MQ=255)
     aactgcGTCTTCACCGGTGGGT‑GGTTCGAAGGCCTGTTTGGTCTGGCAGAGTGTTAAGCAATGCACGAGGCGTTCTGTGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCCGCAGTAACCTTTTATTCAGAACCGGATCTGGCGGCGAACCTGACCGCGCTTGGCGATGAATTACGATTTGTCTTGTTGTCCTCCgg                                                                                                                                                                                                                                      <  2:615425/246‑1 (MQ=255)
      aaTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgc                                                                                                                                                                                                                                     <  1:446061/251‑1 (MQ=255)
       aTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgcg                                                                                                                                                                                                                                    <  1:189716/251‑1 (MQ=255)
         gcGTCTTCACCGGTGAGTTGGT‑CGAAGGCCTGTCTGGTCTGGCAGACAGTGATGCAATGAACGCGTCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTTAACAGAGTCATTGAGCAAGCGCGTGCCGTCAAGAAAGTGGGTGGCTCGCGGGAAGCTGCAGGAACCTTGTATGCATAGCCGTAGCTGGCGGCGAAACTGACCGCGCTGGGCTATGAATTGCGATTTGTCTTGTGGACCTCCTGCGCt                                                                                                                                                                                                                                  <  2:291787/250‑1 (MQ=255)
         aCGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgcgc                                                                                                                                                                                                                                   <  1:56455/250‑1 (MQ=255)
            tcttctCCGGTGAGG‑GGTACGAATGCCTGTTTGGTCTGGCAGACAGTGAATCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGTCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                                                                                                                                                                                                                                <  1:72996/250‑1 (MQ=255)
            ccTTCACCGGGTAGG‑GGTACGAAGGCTTGGTTTGTCTGGCAGACAGTGAAGCAATGAACGGTGCGGTCTGGGACGAGCTGTTGAAAGTGCGGGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCGCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCTGCGCTAcc                                                                                                                                                                                                                               <  2:553044/250‑1 (MQ=255)
               gctccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                                                                                                                                                                                                                            <  2:226253/248‑1 (MQ=255)
                 cccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGc                                                                                                                                                                                                                          <  1:19025/250‑1 (MQ=255)
                  ccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                                                                                                                                                                                                                            >  1:226253/1‑248 (MQ=255)
                    ggTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACACAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGGAACTGACCGCGCTGGGGGATGAATTACGATTTGTCTTTTTGGCCGCCGGCGCTACCGtttgcg                                                                                                                                                                                                                        >  2:387631/1‑246 (MQ=255)
                     gTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGAc                                                                                                                                                                                                                      >  1:90918/1‑251 (MQ=255)
                       gAGT‑GGTACGAATGCCTGTTTTGTCTGGCAGACAGTGAATCAATGATCGAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGAGTGCCGACAAGAAAGTGTGTGGCTCGCTGGAAGCGTCAGTACCCTTTTATGCAGATCCGGAACTGGCGGCGAAACTGACTGCGCTGGGCGATGAATTACGATGTGTCTTTTTGCCCTCCGGCGCTTCCGTTGCAGACta                                                                                                                                                                                                                    <  2:587058/251‑1 (MQ=255)
                                      ccTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctg                                                                                                                                                                                                      <  1:164251/251‑1 (MQ=255)
                                         gTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct                                                                                                                                                                                                    <  1:385727/250‑1 (MQ=255)
                                         gTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct                                                                                                                                                                                                    <  1:151902/250‑1 (MQ=255)
                                          tttGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAGCCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGGTGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgctg                                                                                                                                                                                                   >  1:74582/1‑250 (MQ=255)
                                                  ggCAGACAGTGAAGCCATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcagc                                                                                                                                                                                          <  1:539698/251‑1 (MQ=255)
                                                    cAGACAGTGAAGTAATGAACGATGCGTTCTGGTACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTTGGCGATGAATTACGATTTGTCTTGTTGAACTCCGGCGCTACCGTTGCAGTCTATAACGACGCACCTGCTGATGCTcagcag                                                                                                                                                                                        <  2:367806/251‑1 (MQ=255)
                                                     aGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGCATATAACGACGCACCTGCTGATGCTcagcagg                                                                                                                                                                                       >  2:305179/1‑250 (MQ=255)
                                                     aGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCaga                                                                                                                                                                                       <  1:332016/251‑1 (MQ=255)
                                                         aGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGAGCACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGCTGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGcgcgc                                                                                                                                                                                   >  1:444710/1‑250 (MQ=255)
                                                          gTGAAGCAATGAACGATGCGTTCTGGGTCGAGCTGTTGAAAGTGCTGGGCGAAGTGAACAAAGTCATGGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCGCCGGCGCTACCGTTGCAGACTATAACGACGCGTCTGCTGATGCTCAGCAGAGCGaa                                                                                                                                                                                  <  2:457543/251‑1 (MQ=255)
                                                            gAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATACCGATTTTTCCTGTTGAACTCCGGCGCTACCGTTGCAGACTATAACGACGCACCGGCTGATTCTcagcatacaggtgt                                                                                                                                                                                >  2:77174/1‑242 (MQ=255)
                                                             aaGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTa                                                                                                                                                                               <  1:618071/251‑1 (MQ=255)
                                                              agcaaTGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTAc                                                                                                                                                                              <  1:189089/247‑1 (MQ=255)
                                                                    gAACGATGCGTTCTGGGACGAGCTGTTGACAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGACACTGACCGCGCTGGGCGATGAATTACGGTTTGTCTTGTTGACCTCCGGCGCTACCGTTGCCGACTATAACGACGCACCTGCTGTTGCTCAGCGGCGCGAACTCCTCaa                                                                                                                                                                          >  2:184153/1‑249 (MQ=255)
                                                                      aCGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAggg                                                                                                                                                                      >  1:151107/1‑251 (MQ=255)
                                                                      aCGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAggg                                                                                                                                                                      <  1:537549/251‑1 (MQ=255)
                                                                             gTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAg                                                                                                                                                               <  1:321971/251‑1 (MQ=255)
                                                                                     aCGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACACGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTg                                                                                                                                                       >  1:273308/1‑251 (MQ=255)
                                                                                       gAGCGGTTGAAAGGGCGTGGCGAAGTGAACAAAGGCATTGAGCAAGCTCGTGCCGTCAAGAAAGTGGGTGGCTCGCTGGAAGCGGAAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGCCCGCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGTCTGAAAGTcgttttgag                                                                                                                                                     <  2:226222/251‑8 (MQ=255)
                                                                                        aGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTca                                                                                                                                                                                            <  1:497485/211‑1 (MQ=255)
                                                                                        aGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCACGCTGGAAGCGGCAGGAACCTTGGATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACATACGGCGCTACCGTTGCAGCCTAAAACGACGAACCTGCTGaggcaca                                                                                                                                                                                            >  2:497485/1‑205 (MQ=255)
                                                                                            gTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAg                                                                                                                                                <  1:449085/251‑1 (MQ=255)
                                                                                              ttaaaGTGCGTGGAGAAGTGACCAAAGTCAATGAGCAAGCGCGTGCTGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGTCCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGc                                                                                                                                               <  2:625349/248‑1 (MQ=255)
                                                                                              tGAAAGTGCGGGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGc                                                                                                                                               <  2:488934/250‑1 (MQ=255)
                                                                                              ggAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGcc                                                                                                                                              <  1:152385/250‑1 (MQ=255)
                                                                                               gAAAGTGCGTGGCTAAGTGAACAAAGTCAATGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACTATTTGTCGTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCTCGTTGAGTAATGcc                                                                                                                                              <  2:284073/250‑1 (MQ=255)
                                                                                                 aaGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAg                                                                                                                                                                                                                        <  1:383410/174‑1 (MQ=255)
                                                                                                 aaGTGCGTGGCGAAGTGAAAAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAg                                                                                                                                                                                                                        >  2:383410/1‑174 (MQ=255)
                                                                                                    tGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAggg                                                                                                                                        >  1:614962/1‑250 (MQ=255)
                                                                                                    ggCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGGGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGt                                                                                                                                        <  1:448916/250‑1 (MQ=255)
                                                                                                         ggCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGACGCCAAGAACGTGGGTGGCTCGCTGGAAGCGGCCGAAACCTTGTATGCAGAAACGGAACTGGCGGCGGAACTGACCACGCTGGGCGATGAATTACGATTTGTCTTGTTAACCTACGGCGCTACCGTGGCAGACTATAACGACGCACCTGCTGATGCTAAGCAGTTCGAAGTACTCAAAGGGCTGCAGATCGCAGTGAGTAAAGcctcaggggaga                                                                                                                                    >  2:223751/1‑240 (MQ=255)
                                                                                                           cGAAGTGAACAAAGACATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGACACTGCCCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCCGAGCGACGTACTCAAAGGGCTGAAAGTCGCGTTGAGTACAGCCGAAGGTGAGAAGt                                                                                                                                 >  2:280136/1‑251 (MQ=255)
                                                                                                               gTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGccc                                                                                                                             <  1:305179/251‑1 (MQ=255)
                                                                                                                 gAACTAAGTCATTGAGCAAGAGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGGGGCAGTAACATTGTATGCAGAACCGGAACTGGCGTCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCa                                                                                                                            <  2:357573/250‑1 (MQ=255)
                                                                                                                  aaCAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACg                                                                                                                          >  1:125077/1‑251 (MQ=255)
                                                                                                                          cagtGAGCAAGCGCGTGCCGACAAGAAAGGGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGc                                                                                                                  <  2:115240/248‑1 (MQ=255)
                                                                                                                          cATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGc                                                                                                                  >  1:357764/1‑251 (MQ=255)
                                                                                                                          cATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGc                                                                                                                  >  1:602309/1‑251 (MQ=255)
                                                                                                                          cATTGAGAAAGCGCGGTCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTAGGCAGAACCGTAACTGGCGGCGAAACGGACCGCGCTGGGCGATGAATTACGATTTGTCGTGTTGACCTCCGGCGCGACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTgg                                                                                                                   <  2:115013/250‑1 (MQ=255)
                                                                                                                              gAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACt                                                                                                               >  1:196775/1‑250 (MQ=255)
                                                                                                                                        gTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCCTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGTGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCTCCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTAGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGag                                                                                                    <  2:366305/251‑2 (MQ=255)
                                                                                                                                           ccGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTc                                                                                                 >  1:355781/1‑251 (MQ=255)
                                                                                                                                            cGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCGGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACCCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCg                                                                                                <  2:559479/251‑1 (MQ=255)
                                                                                                                                            cGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCg                                                                                                <  1:410014/251‑1 (MQ=255)
                                                                                                                                             gACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCgg                                                                                               >  1:140980/1‑251 (MQ=255)
                                                                                                                                                   aaaGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGt                                                                                         <  1:554332/251‑1 (MQ=255)
                                                                                                                                                    aaGTGGGCGGCGCGCTGGAAGAGGCAGTAACCTTGTATGCAGAACAGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTCCGCGTTGTCTTGTTGACCTCCGAAGCTGCCGTGGCCGACTCTAACGACGCCCCTGCTGATGCCCAGCAGAGCGCAGTACCCAGAGGTCTGAAGGACGCGTTGAGTAACGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACtcccaccaggcggacggcaaggtg                                                                                        >  2:309758/1‑228 (MQ=255)
                                                                                                                                                          gtggCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGGGGCGGa                                                                                   >  1:562485/1‑250 (MQ=255)
                                                                                                                                                              gttgCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGGGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAAAACg                                                                              <  1:566751/248‑1 (MQ=255)
                                                                                                                                                              cTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGCAACAGCCCGCGCTGGGCGCTGAATTACGCATTGTCTTTTTTCCCTCCGGCGCTACCGTTGCAGACTCTAAAGACGAACATGCTGATGCTCAGCAGCGCGAAGAACTCAACGGGCTGGAAGTCGCGTTTGGAAAAGCCGAAGGTGAGCAGTGCCCACGCTGCTGGCACTACACCCCGGATGTCGGCAATGAGGCGGGACACg                                                                              >  2:657641/1‑251 (MQ=255)
                                                                                                                                                                cGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCa                                                                            <  1:669859/251‑1 (MQ=255)
                                                                                                                                                                  cTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAATGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGGGAGAAGTGCCCACTCTGCTGGCACTACACCCAGGATGTCGGCTAGGTGGCGGAACACgacgg                                                                          >  2:193918/1‑247 (MQ=255)
                                                                                                                                                                     gagtgtggattAACCTCGTATGCAGACTCGGAACTGTAGGCGAAACTGCCCGCGCTGGTCGATGAATCACGATTTTTCTGGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTATTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGAACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAAt                                                                       <  2:156274/241‑1 (MQ=255)
                                                                                                                                                                      aaGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCg                                                                                     <  2:279758/236‑1 (MQ=255)
                                                                                                                                                                      aaGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCg                                                                                     >  1:279758/1‑236 (MQ=255)
                                                                                                                                                                           gCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGACACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGCCTATAACGACGCACCTGCTGATGCTCAGCAGAGCGCAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGACGGGGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCgg                                                                 >  2:153822/1‑251 (MQ=255)
                                                                                                                                                                              gTAACCTTGTATGCAGAACCGGAACTGGCGGCGAACCTGACCTTGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAGGTGCACACGCTGCTGGCACTAAACCCCGGCTGTAGGCAAGGTGGCGGAACGCGCAGAAATTTGCGGccc                                                              >  2:520607/1‑250 (MQ=255)
                                                                                                                                                                                 aCCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAATCGAGTTGAGTAAAGCCGACGGAGAGAAGTGCCCACGCTGCTGGCACTACAACCAGGATGTCGGCAAGGCGGGGGACCACGCCGAAATCTGCGGCCgacg                                                           >  2:646587/1‑248 (MQ=255)
                                                                                                                                                                                     tGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTgtgt                                                        <  1:280136/250‑1 (MQ=255)
                                                                                                                                                                                        aTGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAg                                                     <  1:670625/250‑1 (MQ=255)
                                                                                                                                                                                              aaCCGGAAATGGCGGCGAACCTGACCGCGCTGGGCGATGAATTACGAGTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTc                                             <  2:614962/251‑1 (MQ=255)
                                                                                                                                                                                               aCCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCg                                            <  1:201536/251‑1 (MQ=255)
                                                                                                                                                                                                ccGGAACTGGCGGCGCAACTGACCGCGCTGGGCGATGAATTACGGTTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAAACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGCCGTACTCAAAGGGCTAAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCAGGCACTACACCCAGGATGTCGTCAAGGTGGCGGAACACGCCGAAATCTGCGGCCGATGTGTACGCAAC‑GTCGc                                           >  2:235021/1‑251 (MQ=255)
                                                                                                                                                                                                 cGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGcc                                          <  1:112493/251‑1 (MQ=255)
                                                                                                                                                                                                 cGGAACCGGCGGCGAAACTGACCGCGCTGGGCGAAGAATTACGATTTTTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGcc                                          <  2:485911/251‑1 (MQ=255)
                                                                                                                                                                                                   gAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAAGTACGATTTGTCTTGGTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCcgg                                        <  2:120741/251‑1 (MQ=255)
                                                                                                                                                                                                     actggcggaaggtctggacgcgCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAgg                                                                                          <  1:274970/182‑1 (MQ=255)
                                                                                                                                                                                                      ctggcGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCAGCAAGGTGGCGGAACACGAAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCcggtg                                      >  1:515198/5‑250 (MQ=255)
                                                                                                                                                                                                         gcgtcGAAACGGACCGTGCTGGGCGATGAATTACGTTTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGGCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGATATCTGCGGCCGCTGTGTCAGCAAC‑GGCGCcggtgacgg                                  <  2:424326/247‑1 (MQ=255)
                                                                                                                                                                                                          cGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCcggtgacggt                                 <  1:387631/251‑1 (MQ=255)
                                                                                                                                                                                                                aaCTGAACGCGATGGGCGATGAATTACGATTTCTCTTGATAACCTCCGGCGCTACAGTTGCAGACTATAACGACGAACCTGCTGATACTCAGCAGAGCGAAGTACTCAAAGGGCTGACAGTCGCGTTGAGTACAGCCGAAGTTGCGAAGAGCACACGCTGCTGGCACTACACCCAGGAGGTCGGCAAGGTGGCGGAACACGCCGCAACCTGGGGCAGCTGTGTCAGAACC‑GTCGACGGAGAAGGGGaaaa                            >  2:418873/1‑250 (MQ=255)
                                                                                                                                                                                                                         cgcTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGttt                  >  1:471996/1‑251 (MQ=255)
                                                                                                                                                                                                                           cTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGc                >  1:510468/1‑251 (MQ=255)
                                                                                                                                                                                                                             gggCGATGAATTACGATTTGTCTTGTTGACCTCCGTCGATCCCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGGCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACTCCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCt              <  2:444710/251‑1 (MQ=255)
                                                                                                                                                                                                                             gggCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCt              >  1:179505/1‑251 (MQ=255)
                                                                                                                                                                                                                                  aTGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCtgatga         <  1:425648/251‑1 (MQ=255)
                                                                                                                                                                                                                                    gAATTACGATTTGTCTTGTTGACCTCCTGCGCTACCGTTGCAGAATATAACGACGCACCTGCTGAGGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTTTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGt       <  2:450723/251‑1 (MQ=255)
                                                                                                                                                                                                                                    gAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGt       >  1:199132/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aaTTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTc      >  1:434387/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aaTTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTc      >  2:406596/1‑251 (MQ=255)
                                                                                                                                                                                                                                        tACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAGAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTCAAt   >  1:128130/1‑251 (MQ=255)
                                                                                                                                                                                                                                         aCGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTCAATc  <  1:169279/251‑1 (MQ=255)
                                                                                                                                                                                                                                         aCGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGACGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTCAATc  >  2:631205/1‑251 (MQ=255)
                                                                                                                                                                                                                                         aCGATTTGTCTTGGTGACCTCCGGCGCGACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTCAATc  <  2:471996/251‑1 (MQ=255)
                                                                                                                                                                                                                                         aCGATTTGGCTTGTTGACCTCCGGCGCTACCGTTGCCGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGACAGACGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACACAGGATGTCGGCAAGGTGGCAGAACACGCAGAAATCTGCGGCCGATGTGTCAG‑AACAGTCCCCGGTGAAGGGGAAAAACGTAAGTTTGCCTGATGAGTCAATc  >  2:481290/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
GTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAAC‑GTCGCCGGTGACGGTGAAAAACGTAAGTTTGCCTGATGAGTCAATC  >  NC_000913/24735‑25217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑