Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 24,935 T→G S849A (TCG→GCG)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091324,9350TG93.5% 309.1 / ‑1.1 108S849A (TCG→GCG) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base G (40/61);  total (43/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

Only 100 of 108 total aligned reads displayed.

CCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGCCGG  >  NC_000913/24688‑25179
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
ccTCTCCTTCACCGCTGATGAAGTGTGGGACTTCCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCgg                                                                                                                                                                                                                                                   >  1:226222/1‑251 (MQ=255)
    tcCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGGGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGa                                                                                                                                                                                                                                                >  1:646493/1‑250 (MQ=255)
           ccgcttgggaagtggtgggCTACCGTCCGTGCGAACGTGAAAAATGAGGCTTCGCCGGGTAGT‑GGTACGAAGGCCTGTCTGGTCGGGCAGACAGTGACGCAATGAACGAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCATGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTggcc                                                                                                                                                                                                                                        <  2:422106/235‑4 (MQ=255)
           ccGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGAcc                                                                                                                                                                                                                                        <  1:606424/251‑1 (MQ=255)
                 atgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTg                                                                                                                                                                                                                                  >  1:357573/1‑251 (MQ=255)
                  tgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAACCTGACCGCGCTgg                                                                                                                                                                                                                                 >  2:603179/1‑251 (MQ=255)
                    aaGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATCCGTCTTCCCCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGGGTAGCAATGAAAGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGAGCGTGCCGCCAAGACAGTGGGTGGCCCGCTGGAAGCGGCAGGAACCTTGTATGCAGAACAGGAACTGGCGGGTAAACTGACCGCGCTgggg                                                                                                                                                                                                                               >  2:368373/1‑250 (MQ=255)
                        gtgGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGAt                                                                                                                                                                                                                            <  1:603179/250‑1 (MQ=255)
                          ggggCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGa                                                                                                                                                                                                                          <  1:631888/250‑1 (MQ=255)
                           gggCTTCCTGCCGGGCGAACGTGAAAACTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAAt                                                                                                                                                                                                                        <  2:461197/251‑1 (MQ=255)
                           gggCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAGAGTGCGTGGCGAAGAGAACAAAGTCATTGAGCAAGCGCGAGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGAGGCGAGACTGACCGCGCTGGGCGATGAAt                                                                                                                                                                                                                        >  2:410014/1‑251 (MQ=255)
                                    gCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGAATACAGGGAAGCAATGAACGATGCGTTCGGGGACGAGCTGGTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCTGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGAtt                                                                                                                                                                                                                 <  2:600778/249‑1 (MQ=255)
                                     ccGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGt                                                                                                                                                                                                              >  1:450723/1‑251 (MQ=255)
                                     ccGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGt                                                                                                                                                                                                              >  1:28804/1‑251 (MQ=255)
                                     ccGGGCGAACGTGAAAAATACGTCTACTCCGGTGAGT‑TGAACGAAGGTCTGTGTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGGACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGTGCGATGAATTACGATTTGt                                                                                                                                                                                                              <  2:284957/251‑1 (MQ=255)
                                      cGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTGCGATTTGTc                                                                                                                                                                                                             <  1:498618/251‑1 (MQ=255)
                                        ggCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTctt                                                                                                                                                                                                           <  2:4112/251‑3 (MQ=255)
                                         gCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGGTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcttg                                                                                                                                                                                                          <  2:28804/251‑4 (MQ=255)
                                               ggtaaaaaTTCGTCTTCGCCTGTGAGT‑GGTACGCAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGAGGTGTTCTGGGACGTGATGTTGAAAGTGCGTGTCGTAGTGAACAAAGTCACTGAGCAAGCGCGTGCAGACCAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTTTCGTCGAAATTGACCGGGCTGGGCGATGAATTACGATTTGTCttgttgccc                                                                                                                                                                                                    <  1:541477/248‑4 (MQ=255)
                                                 gAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTTGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTc                                                                                                                                                                                                  <  1:421707/251‑1 (MQ=255)
                                                   aaaaTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcttggttacctctg                                                                                                                                                                                                >  2:385727/1‑238 (MQ=255)
                                                    aactgcGTCTTCACCGGTGGGT‑GGTTCGAAGGCCTGTTTGGTCTGGCAGAGTGTTAAGCAATGCACGAGGCGTTCTGTGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCCGCAGTAACCTTTTATTCAGAACCGGATCTGGCGGCGAACCTGACCGCGCTTGGCGATGAATTACGATTTGTCTTGTTGTCCTCCgg                                                                                                                                                                                               <  2:615425/246‑1 (MQ=255)
                                                     aaTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgc                                                                                                                                                                                              <  1:446061/251‑1 (MQ=255)
                                                      aTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgcg                                                                                                                                                                                             <  1:189716/251‑1 (MQ=255)
                                                        gcGTCTTCACCGGTGAGTTGGT‑CGAAGGCCTGTCTGGTCTGGCAGACAGTGATGCAATGAACGCGTCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTTAACAGAGTCATTGAGCAAGCGCGTGCCGTCAAGAAAGTGGGTGGCTCGCGGGAAGCTGCAGGAACCTTGTATGCATAGCCGTAGCTGGCGGCGAAACTGACCGCGCTGGGCTATGAATTGCGATTTGTCTTGTGGACCTCCTGCGCt                                                                                                                                                                                           <  2:291787/250‑1 (MQ=255)
                                                        aCGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgcgc                                                                                                                                                                                            <  1:56455/250‑1 (MQ=255)
                                                           tcttctCCGGTGAGG‑GGTACGAATGCCTGTTTGGTCTGGCAGACAGTGAATCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGTCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                                                                                                                                                                                         <  1:72996/250‑1 (MQ=255)
                                                           ccTTCACCGGGTAGG‑GGTACGAAGGCTTGGTTTGTCTGGCAGACAGTGAAGCAATGAACGGTGCGGTCTGGGACGAGCTGTTGAAAGTGCGGGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCGCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCTGCGCTAcc                                                                                                                                                                                        <  2:553044/250‑1 (MQ=255)
                                                              gctccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                                                                                                                                                                                     <  2:226253/248‑1 (MQ=255)
                                                                cccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGc                                                                                                                                                                                   <  1:19025/250‑1 (MQ=255)
                                                                   ggTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACACAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGGAACTGACCGCGCTGGGGGATGAATTACGATTTGTCTTTTTGGCCGCCGGCGCTACCGtttgcg                                                                                                                                                                                 >  2:387631/1‑246 (MQ=255)
                                                                    gTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGAc                                                                                                                                                                               >  1:90918/1‑251 (MQ=255)
                                                                      gAGT‑GGTACGAATGCCTGTTTTGTCTGGCAGACAGTGAATCAATGATCGAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGAGTGCCGACAAGAAAGTGTGTGGCTCGCTGGAAGCGTCAGTACCCTTTTATGCAGATCCGGAACTGGCGGCGAAACTGACTGCGCTGGGCGATGAATTACGATGTGTCTTTTTGCCCTCCGGCGCTTCCGTTGCAGACta                                                                                                                                                                             <  2:587058/251‑1 (MQ=255)
                                                                                     ccTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctg                                                                                                                                                               <  1:164251/251‑1 (MQ=255)
                                                                                        gTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct                                                                                                                                                             <  1:151902/250‑1 (MQ=255)
                                                                                         tttGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAGCCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGGTGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgctg                                                                                                                                                            >  1:74582/1‑250 (MQ=255)
                                                                                                 ggCAGACAGTGAAGCCATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcagc                                                                                                                                                   <  1:539698/251‑1 (MQ=255)
                                                                                                   cAGACAGTGAAGTAATGAACGATGCGTTCTGGTACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTTGGCGATGAATTACGATTTGTCTTGTTGAACTCCGGCGCTACCGTTGCAGTCTATAACGACGCACCTGCTGATGCTcagcag                                                                                                                                                 <  2:367806/251‑1 (MQ=255)
                                                                                                    aGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGCATATAACGACGCACCTGCTGATGCTcagcagg                                                                                                                                                >  2:305179/1‑250 (MQ=255)
                                                                                                    aGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCaga                                                                                                                                                <  1:332016/251‑1 (MQ=255)
                                                                                                        aGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGAGCACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGCTGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGcgcgc                                                                                                                                            >  1:444710/1‑250 (MQ=255)
                                                                                                         gTGAAGCAATGAACGATGCGTTCTGGGTCGAGCTGTTGAAAGTGCTGGGCGAAGTGAACAAAGTCATGGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCGCCGGCGCTACCGTTGCAGACTATAACGACGCGTCTGCTGATGCTCAGCAGAGCGaa                                                                                                                                           <  2:457543/251‑1 (MQ=255)
                                                                                                           gAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATACCGATTTTTCCTGTTGAACTCCGGCGCTACCGTTGCAGACTATAACGACGCACCGGCTGATTCTcagcatacaggtgt                                                                                                                                         >  2:77174/1‑242 (MQ=255)
                                                                                                            aaGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTa                                                                                                                                        <  1:618071/251‑1 (MQ=255)
                                                                                                             agcaaTGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTAc                                                                                                                                       <  1:189089/247‑1 (MQ=255)
                                                                                                                   gAACGATGCGTTCTGGGACGAGCTGTTGACAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGACACTGACCGCGCTGGGCGATGAATTACGGTTTGTCTTGTTGACCTCCGGCGCTACCGTTGCCGACTATAACGACGCACCTGCTGTTGCTCAGCGGCGCGAACTCCTCaa                                                                                                                                   >  2:184153/1‑249 (MQ=255)
                                                                                                                     aCGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAggg                                                                                                                               <  1:537549/251‑1 (MQ=255)
                                                                                                                     aCGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAggg                                                                                                                               >  1:151107/1‑251 (MQ=255)
                                                                                                                            gTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAg                                                                                                                        <  1:321971/251‑1 (MQ=255)
                                                                                                                                  ggACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTCACCTTGTATGCAGAACCGGAAATGGCGGCGAAACTGACCGCGCTAGGCGATGAATTACGATTTGTCTTGTTTACCTCCGACGCTACCGTTGCATACTATAACGACGACCCTGCCGATGCTCAGCAGAGCGAAGAACTCAAGGTGCTGAAAGTcgcgg                                                                                                                  >  2:241842/1‑250 (MQ=255)
                                                                                                                                  ggACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGa                                                                                                                                                                                                                   <  2:558585/154‑1 (MQ=255)
                                                                                                                                  ggACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGa                                                                                                                                                                                                                   >  1:558585/1‑154 (MQ=255)
                                                                                                                                    aCGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACACGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTg                                                                                                                >  1:273308/1‑251 (MQ=255)
                                                                                                                                      gAGCGGTTGAAAGGGCGTGGCGAAGTGAACAAAGGCATTGAGCAAGCTCGTGCCGTCAAGAAAGTGGGTGGCTCGCTGGAAGCGGAAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGCCCGCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGTCTGAAAGTcgttttgag                                                                                                              <  2:226222/251‑8 (MQ=255)
                                                                                                                                       aGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTca                                                                                                                                                     <  1:497485/211‑1 (MQ=255)
                                                                                                                                       aGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCACGCTGGAAGCGGCAGGAACCTTGGATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACATACGGCGCTACCGTTGCAGCCTAAAACGACGAACCTGCTGaggcaca                                                                                                                                                     >  2:497485/1‑205 (MQ=255)
                                                                                                                                           gTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAg                                                                                                         <  1:449085/251‑1 (MQ=255)
                                                                                                                                             ttaaaGTGCGTGGAGAAGTGACCAAAGTCAATGAGCAAGCGCGTGCTGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGTCCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGc                                                                                                        <  2:625349/248‑1 (MQ=255)
                                                                                                                                             tGAAAGTGCGGGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGc                                                                                                        <  2:488934/250‑1 (MQ=255)
                                                                                                                                             ggAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGcc                                                                                                       <  1:152385/250‑1 (MQ=255)
                                                                                                                                              gAAAGTGCGTGGCTAAGTGAACAAAGTCAATGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACTATTTGTCGTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCTCGTTGAGTAATGcc                                                                                                       <  2:284073/250‑1 (MQ=255)
                                                                                                                                                aaGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAg                                                                                                                                                                                 <  1:383410/174‑1 (MQ=255)
                                                                                                                                                aaGTGCGTGGCGAAGTGAAAAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAg                                                                                                                                                                                 >  2:383410/1‑174 (MQ=255)
                                                                                                                                                 aGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTAc                                                                                                                                                                                                                     <  2:347699/137‑1 (MQ=255)
                                                                                                                                                 aGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTAc                                                                                                                                                                                                                     >  1:347699/1‑137 (MQ=255)
                                                                                                                                                   tGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAggg                                                                                                 >  1:614962/1‑250 (MQ=255)
                                                                                                                                                   ggCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGGGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGt                                                                                                 <  1:448916/250‑1 (MQ=255)
                                                                                                                                                        ggCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGACGCCAAGAACGTGGGTGGCTCGCTGGAAGCGGCCGAAACCTTGTATGCAGAAACGGAACTGGCGGCGGAACTGACCACGCTGGGCGATGAATTACGATTTGTCTTGTTAACCTACGGCGCTACCGTGGCAGACTATAACGACGCACCTGCTGATGCTAAGCAGTTCGAAGTACTCAAAGGGCTGCAGATCGCAGTGAGTAAAGcctcaggggaga                                                                                             >  2:223751/1‑240 (MQ=255)
                                                                                                                                                          cGAAGTGAACAAAGACATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGACACTGCCCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCCGAGCGACGTACTCAAAGGGCTGAAAGTCGCGTTGAGTACAGCCGAAGGTGAGAAGt                                                                                          >  2:280136/1‑251 (MQ=255)
                                                                                                                                                              gTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGccc                                                                                      <  1:305179/251‑1 (MQ=255)
                                                                                                                                                                gAACTAAGTCATTGAGCAAGAGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGGGGCAGTAACATTGTATGCAGAACCGGAACTGGCGTCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCa                                                                                     <  2:357573/250‑1 (MQ=255)
                                                                                                                                                                 aaCAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACg                                                                                   >  1:125077/1‑251 (MQ=255)
                                                                                                                                                                         cagtGAGCAAGCGCGTGCCGACAAGAAAGGGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGc                                                                           <  2:115240/248‑1 (MQ=255)
                                                                                                                                                                         cATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGc                                                                           >  1:357764/1‑251 (MQ=255)
                                                                                                                                                                         cATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGc                                                                           >  1:602309/1‑251 (MQ=255)
                                                                                                                                                                         cATTGAGAAAGCGCGGTCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTAGGCAGAACCGTAACTGGCGGCGAAACGGACCGCGCTGGGCGATGAATTACGATTTGTCGTGTTGACCTCCGGCGCGACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTgg                                                                            <  2:115013/250‑1 (MQ=255)
                                                                                                                                                                             gAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACt                                                                        >  1:196775/1‑250 (MQ=255)
                                                                                                                                                                                          ccGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTc                                                          >  1:355781/1‑251 (MQ=255)
                                                                                                                                                                                           cGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCGGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACCCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCg                                                         <  2:559479/251‑1 (MQ=255)
                                                                                                                                                                                           cGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCg                                                         <  1:410014/251‑1 (MQ=255)
                                                                                                                                                                                            gACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCgg                                                        >  1:140980/1‑251 (MQ=255)
                                                                                                                                                                                                   aaGTGGGCGGCGCGCTGGAAGAGGCAGTAACCTTGTATGCAGAACAGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTCCGCGTTGTCTTGTTGACCTCCGAAGCTGCCGTGGCCGACTCTAACGACGCCCCTGCTGATGCCCAGCAGAGCGCAGTACCCAGAGGTCTGAAGGACGCGTTGAGTAACGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACtcccaccaggcggacggcaaggtg                                                 >  2:309758/1‑228 (MQ=255)
                                                                                                                                                                                                         gtggCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGGGGCGGa                                            >  1:562485/1‑250 (MQ=255)
                                                                                                                                                                                                             gttgCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGGGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAAAACg                                       <  1:566751/248‑1 (MQ=255)
                                                                                                                                                                                                             cTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGCAACAGCCCGCGCTGGGCGCTGAATTACGCATTGTCTTTTTTCCCTCCGGCGCTACCGTTGCAGACTCTAAAGACGAACATGCTGATGCTCAGCAGCGCGAAGAACTCAACGGGCTGGAAGTCGCGTTTGGAAAAGCCGAAGGTGAGCAGTGCCCACGCTGCTGGCACTACACCCCGGATGTCGGCAATGAGGCGGGACACg                                       >  2:657641/1‑251 (MQ=255)
                                                                                                                                                                                                               cGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCa                                     <  1:669859/251‑1 (MQ=255)
                                                                                                                                                                                                                 cTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGc                                      >  1:446005/1‑248 (MQ=255)
                                                                                                                                                                                                                 cTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAATGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGGGAGAAGTGCCCACTCTGCTGGCACTACACCCAGGATGTCGGCTAGGTGGCGGAACACgacgg                                   >  2:193918/1‑247 (MQ=255)
                                                                                                                                                                                                                    gagtgtggattAACCTCGTATGCAGACTCGGAACTGTAGGCGAAACTGCCCGCGCTGGTCGATGAATCACGATTTTTCTGGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTATTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGAACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAAt                                <  2:156274/241‑1 (MQ=255)
                                                                                                                                                                                                                     aaGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCg                                              >  1:279758/1‑236 (MQ=255)
                                                                                                                                                                                                                     aaGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCg                                              <  2:279758/236‑1 (MQ=255)
                                                                                                                                                                                                                          gCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGACACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGCCTATAACGACGCACCTGCTGATGCTCAGCAGAGCGCAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGACGGGGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCgg                          >  2:153822/1‑251 (MQ=255)
                                                                                                                                                                                                                                aCCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAATCGAGTTGAGTAAAGCCGACGGAGAGAAGTGCCCACGCTGCTGGCACTACAACCAGGATGTCGGCAAGGCGGGGGACCACGCCGAAATCTGCGGCCgacg                    >  2:646587/1‑248 (MQ=255)
                                                                                                                                                                                                                                    tGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTgtgt                 <  1:280136/250‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCCGGAAATGGCGGCGAACCTGACCGCGCTGGGCGATGAATTACGAGTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTc       <  2:614962/251‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCg      <  1:201536/251‑1 (MQ=255)
                                                                                                                                                                                                                                               ccGGAACTGGCGGCGCAACTGACCGCGCTGGGCGATGAATTACGGTTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAAACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGCCGTACTCAAAGGGCTAAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCAGGCACTACACCCAGGATGTCGTCAAGGTGGCGGAACACGCCGAAATCTGCGGCCGATGTGTACGCAACGTCGc     >  2:235021/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGcc    <  1:112493/251‑1 (MQ=255)
                                                                                                                                                                                                                                                cGGAACCGGCGGCGAAACTGACCGCGCTGGGCGAAGAATTACGATTTTTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGcc    <  2:485911/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAAGTACGATTTGTCTTGGTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGCcgg  <  2:120741/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
CCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGCCGG  >  NC_000913/24688‑25179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑