Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,781	C→T	G797G (GGC→GGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,781	0	C	T	97.9%	289.0 / NA	97	G797G (GGC→GGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/53);  minor base G (0/2);  total (42/55)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

GCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTG  >  NC_000913/24538‑25025                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gcgcTTCTGCCCCGTAGGTATGGGTTCCTCCTACCTCGACATCATCAAAGCCCGCCAGTCCACCTCCAAAGCGGACAGCGTGGCGCGGCGTAGCTGCCAGACTGCGCTGTAGCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGGCTGGCAg                                                                                                                                                                                                                                                <  2:660385/251‑1 (MQ=255)  cgcTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGa                                                                                                                                                                                                                                               <  1:149640/251‑1 (MQ=255)            ccGTTGAGATGGGTTCCTTCTCCCTCGACACCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGGGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                                                                                                                                                                                     <  2:305606/251‑2 (MQ=255)             cGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                                                                                                                                                                                     >  1:485911/1‑249 (MQ=255)              gTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGCACGTCAGTACAACGCCAAAGCGGACAGCGTGCCGCGTTGTAGCTGCCAGACCGCGCTCTATCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTACTGAAGTGTGGGGCTACCTGCCGGGCGACCGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGCTTGGTCTGGCAGACAGTGAAGcatt                                                                                                                                                                                                                                   >  1:286356/1‑248 (MQ=255)                tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑Tg                                                                                                                                                                                                                                 >  2:618071/1‑250 (MQ=255)                  agaTGGGTTCCTTCTACCTCGACATCATCAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCCCAGAAGCGCGGGGTCGCTGGAAGGCACCAATCCTCT‑CCTTCACCGCTGATGTAGTGTGGGGCTACCTGCCGGTCGAGCGTGAAAAATACGTCTTCACCGGGGAGT‑GGTCAGAAGGACTGTTTGTTCTGGCAGACAGTgcaacaatgaac                                                                                                                                                                                                                               >  2:539698/1‑240 (MQ=255)                  agaTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAc                                                                                                                                                                                                                              <  1:651974/251‑1 (MQ=255)                  aactGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGAGCGTCGTAGCTGCCAGACTGCGCTGGATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTTGCAGACCGTGAAGCAA‑TGAAc                                                                                                                                                                                                                              <  2:444006/248‑1 (MQ=255)                        gTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGGGTGGGGCTACCTGCCGGGCGCACGTGAAAAATACGTCTTCACCGGTGGGG‑GGTACGAAGGCCGGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAcaatacg                                                                                                                                                                                                                        >  2:19025/1‑245 (MQ=255)                         ttCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGT‑GGTACGCAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGt                                                                                                                                                                                                                       >  1:587058/1‑251 (MQ=255)                               cTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTggg                                                                                                                                                                                                                 >  1:433730/1‑251 (MQ=255)                                        aattaccaAAGACCGACAGTACACTGCCAAAGCGGACAGCGTGGATCGTCGTAGCTGCAAGACTGCGCTGTATCCCATCGCAGAATCGCTGGTTCGCTGGATGGCACCATTCCGCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTGGGGCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGt                                                                                                                                                                                                        <  2:386448/245‑1 (MQ=255)                                           catcaAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTg                                                                                                                                                                                                      >  1:55189/1‑250 (MQ=255)                                              caAAGCCCGTCAGAACCCCGCCAAAGCGGACAGCGTGGCGCTTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGaa                                                                                                                                                                                                    <  2:433730/249‑1 (MQ=255)                                              caAAGACCGGCAGAACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAGCTGCCAGAGTGCGCTGTATAACATCGCAGCAGCGCTGGCACGCTGGAGGGCACTAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTGCATGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAG‑GACAGAACGATGCGTTCGGGGACGATGTGTTGac                                                                                                                                                                                                    >  2:51373/1‑248 (MQ=255)                                              aaaaGACCGTCCGTACTCCGCCAAAGCGGACAGCGGGGCGCGGCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAg                                                                                                                                                                                                  <  2:327459/250‑1 (MQ=255)                                                aaGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGACCGTGTAAAATACGTCTTCACCGGGGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGGAAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTg                                                                                                                                                                                                >  2:201536/1‑251 (MQ=255)                                                 agtccGTCAGTACACCGCCAAGGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGc                                                                                                                                                                                               <  1:452336/248‑1 (MQ=255)                                                  gACCGGCAGTCCCCCGCCAAATCGGTCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCg                                                                                                                                                                                              <  2:502209/251‑1 (MQ=255)                                                    ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                                                                                                                                                            >  1:512676/1‑251 (MQ=255)                                                     cGTCAGTACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGACCGATGAGTTCTGGGCCGAGCTGTTGAAAGCGCGTgg                                                                                                                                                                                           >  2:90250/1‑251 (MQ=255)                                                       gcagaccccccacAAAGCGGCCAGCGTGGCGCGTAGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGGTTGATGGCACCAATCCGCC‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                                                                                                                                                                                           <  2:17430/237‑1 (MQ=255)                                                        cAGTACACCGCCAAAGCGTACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCTTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                                                                                                                                                                         <  1:440364/250‑1 (MQ=255)                                                         aGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGa                                                                                                                                                                                        >  1:120741/1‑250 (MQ=255)                                                            accccGCCAAAGCGCACAGCGTGGCGCGCCGTAGCTGCCAGACTGCGCTGTATCACATCGCATAAGCGCTGGTTCGCTGGATGGCTCCAA‑CCCCTGCCTTCACCGCTGATGAAGTGTGGGGCCACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCGGGGACGAGCTGTTGAAAGGGCGTGGCGAAGt                                                                                                                                                                                     <  2:55189/247‑1 (MQ=255)                                                             cacCGCCAAAGCGGACAGGGGGGCGGGGCGTCGCTGCACGACTGCGCTGTATCCAATCGCAGAAGCGCTGGGTCGCGGGATGGCACCAATCTTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTGGAAAGTGCGTGGCGAAGTGa                                                                                                                                                                                   <  2:110517/251‑1 (MQ=255)                                                              cccGCCAAAGCGGCCTGCGGGTCGCGTCGTAGCTGCCAGACTGCGCTTTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa                                                                                                                                                                                  <  2:73324/250‑1 (MQ=255)                                                              cccGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCATACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa                                                                                                                                                                                  <  1:389554/250‑1 (MQ=255)                                                                     aaGCGGACAGCGTAGCGAGACGTAGCTGCCAGACTGAGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGAACAAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGTGGAGAAGTGACCACAGTc                                                                                                                                                                           >  2:449085/1‑251 (MQ=255)                                                                          gACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                                                                                                                                                                       >  1:296392/1‑250 (MQ=255)                                                                           aCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGTCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGAGGCGAAGTGGACAAAGTCATTg                                                                                                                                                                       >  2:554332/1‑249 (MQ=255)                                                                           aCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAg                                                                                                                                                                     <  1:432931/251‑1 (MQ=255)                                                                                 tgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcg                                                                                                                                                               >  1:115240/1‑251 (MQ=255)                                                                                     gcgTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTg                                                                                                                                                           <  1:246611/251‑1 (MQ=255)                                                                                         cgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTAAGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGGGCCg                                                                                                                                                        >  1:149773/1‑250 (MQ=255)                                                                                          gtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGCTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGAc                                                                                                                                                      >  1:106661/1‑251 (MQ=255)                                                                                                gCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                                                                                                                                                >  2:344857/1‑235 (MQ=255)                                                                                                gCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                                                                                                                                                <  1:344857/235‑1 (MQ=255)                                                                                                   aGACTGCGCTGTAACACATCGCAGAAGCGATGGATCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGATGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGCGCAAACGCGGGCCGACAAGAACgtg                                                                                                                                             >  2:669859/1‑251 (MQ=255)                                                                                                             gtatCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGc                                                                                                                                   >  1:488934/2‑251 (MQ=255)                                                                                                             gtatCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGc                                                                                                                                   >  1:461197/2‑251 (MQ=255)                                                                                                               atCACATCGCAGAAGCGCTGGTTCGCTTGATGGCACCAATCCTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCtt                                                                                                                                 <  1:289215/251‑2 (MQ=255)                                                                                                               atCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTg                                                                                                                                 <  1:289189/251‑1 (MQ=255)                                                                                                                   catggCAGAAGCGATGGTTCTCTGGATGGCACCAATCCTCG‑TCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAg                                                                                                                             <  2:626154/247‑1 (MQ=255)                                                                                                                    aTCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGc                                                                                                                            >  2:164251/1‑251 (MQ=255)                                                                                                                      cGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCgg                                                                                                                          <  1:450383/251‑1 (MQ=255)                                                                                                                        cAGAAGCGCTGGTTCGCTGGATGGCTCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCa                                                                                                                        <  2:89361/251‑1 (MQ=255)                                                                                                                         aGAAGCGCTGGGTCGCTGGATGGCATCAATCCACG‑CCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAg                                                                                                                       <  2:168713/251‑1 (MQ=255)                                                                                                                                 tgggtcgcTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGCACGATGCGTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGACGCGGCAGTAACCttt                                                                                                               >  2:189716/6‑250 (MQ=255)                                                                                                                                      cgctggacatcACCAATCCTCT‑CCTTCACCGCTGATGACGTGTGGACCTACCTGCCGGACGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTAGGAAGGCCTTTTTGGTCTGGCAGTCGGCGAAGCAA‑TGAACGATGCGTTCTGTGACGGGCGGTTGCTAGTGCGTGGCGCCGTGAATAAGGACATTGAGCAAGCGCGTGCCGAGACGACAGTGGGTGGCTCGCTGGAAGCGGCAGTCACCtttgatgc                                                                                                          >  2:566751/11‑245 (MQ=255)                                                                                                                                      cgcTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGCCGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGc                                                                                                          >  2:321971/1‑251 (MQ=255)                                                                                                                                       gcTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCa                                                                                                         <  1:16036/251‑1 (MQ=255)                                                                                                                                        cTGGATGGCACCAATCATCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACACGAAAGAGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGc                                                                                                          >  2:557419/1‑249 (MQ=255)                                                                                                                                               gcgccAATCCGCT‑CCTGCACCTCTGAGGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCGTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGTCAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGa                                                                                                 <  2:586418/248‑1 (MQ=255)                                                                                                                                               gCACCAATCCTCT‑CCTTCACCGCTGCTGCAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCgg                                                                                                  >  1:559479/1‑250 (MQ=255)                                                                                                                                                   caagccttt‑cCTTCCCCGCAGATGAAGGGTTGGTCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGGACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTATCCTTGTATGCAGAACCGGAACTg                                                                                             <  2:668367/243‑1 (MQ=255)                                                                                                                                                       ccTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGATGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTggcg                                                                                          <  1:416601/250‑4 (MQ=255)                                                                                                                                                       ccTCT‑CCTTCACCGCTGATGAAGTGTGGGACTTCCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCgg                                                                                         >  1:226222/1‑251 (MQ=255)                                                                                                                                                           t‑cCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGGGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGa                                                                                      >  1:646493/1‑250 (MQ=255)                                                                                                                                                                   ccgcttgggaagtggtgggCTACCGTCCGTGCGAACGTGAAAAATGAGGCTTCGCCGGGTAGT‑GGTACGAAGGCCTGTCTGGTCGGGCAGACAGTGACGCAA‑TGAACGAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCATGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTggcc                                                                              <  2:422106/235‑4 (MQ=255)                                                                                                                                                                   ccGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGAcc                                                                              <  1:606424/251‑1 (MQ=255)                                                                                                                                                                         atgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTg                                                                        >  1:357573/1‑251 (MQ=255)                                                                                                                                                                          tgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAACCTGACCGCGCTgg                                                                       >  2:603179/1‑251 (MQ=255)                                                                                                                                                                            aaGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATCCGTCTTCCCCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGGGTAGCAA‑TGAAAGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGAGCGTGCCGCCAAGACAGTGGGTGGCCCGCTGGAAGCGGCAGGAACCTTGTATGCAGAACAGGAACTGGCGGGTAAACTGACCGCGCTgggg                                                                     >  2:368373/1‑250 (MQ=255)                                                                                                                                                                                gtgGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGAt                                                                  <  1:603179/250‑1 (MQ=255)                                                                                                                                                                                  ggggCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGa                                                                <  1:631888/250‑1 (MQ=255)                                                                                                                                                                                   gggCTTCCTGCCGGGCGAACGTGAAAACTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAAt                                                              <  2:461197/251‑1 (MQ=255)                                                                                                                                                                                   gggCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAGAGTGCGTGGCGAAGAGAACAAAGTCATTGAGCAAGCGCGAGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGAGGCGAGACTGACCGCGCTGGGCGATGAAt                                                              >  2:410014/1‑251 (MQ=255)                                                                                                                                                                                            gCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGAATACAGGGAAGCAA‑TGAACGATGCGTTCGGGGACGAGCTGGTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCTGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGAtt                                                       <  2:600778/249‑1 (MQ=255)                                                                                                                                                                                             ccGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGt                                                    >  1:28804/1‑251 (MQ=255)                                                                                                                                                                                             ccGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGt                                                    >  1:450723/1‑251 (MQ=255)                                                                                                                                                                                             ccGGGCGAACGTGAAAAATACGTCTACTCCGGTGAGT‑TGAACGAAGGTCTGTGTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGGACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGTGCGATGAATTACGATTTGt                                                    <  2:284957/251‑1 (MQ=255)                                                                                                                                                                                              cGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTGCGATTTGTc                                                   <  1:498618/251‑1 (MQ=255)                                                                                                                                                                                                ggCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTctt                                                 <  2:4112/251‑3 (MQ=255)                                                                                                                                                                                                 gtgtaCGTGAAAAATTCATCTTCACCGTTGAGG‑GGGACGAAGGCTTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTGGAAAGTGCGTGGCGAAGTGAACAAATTCAGTGAGCAAGCGCGTTCCGACAATAAAGTGGGTGGCTCGCTGGAAGCGGCATTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAGCTGACCGCGCTGGGCGATGAATTACGATTTGTcttg                                                <  2:227343/247‑4 (MQ=255)                                                                                                                                                                                                 gCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcttg                                                <  2:28804/251‑4 (MQ=255)                                                                                                                                                                                                       ggtaaaaaTTCGTCTTCGCCTGTGAGT‑GGTACGCAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGAGGTGTTCTGGGACGTGATGTTGAAAGTGCGTGTCGTAGTGAACAAAGTCACTGAGCAAGCGCGTGCAGACCAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTTTCGTCGAAATTGACCGGGCTGGGCGATGAATTACGATTTGTCttgttgccc                                          <  1:541477/248‑4 (MQ=255)                                                                                                                                                                                                         gAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTTGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTc                                        <  1:421707/251‑1 (MQ=255)                                                                                                                                                                                                           aaaaTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcttggttacctctg                                      >  2:385727/1‑238 (MQ=255)                                                                                                                                                                                                            aactgcGTCTTCACCGGTGGGT‑GGTTCGAAGGCCTGTTTGGTCTGGCAGAGTGTTAAGCAA‑TGCACGAGGCGTTCTGTGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCCGCAGTAACCTTTTATTCAGAACCGGATCTGGCGGCGAACCTGACCGCGCTTGGCGATGAATTACGATTTGTCTTGTTGTCCTCCgg                                     <  2:615425/246‑1 (MQ=255)                                                                                                                                                                                                             aaTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgc                                    <  1:446061/251‑1 (MQ=255)                                                                                                                                                                                                              aTACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgcg                                   <  1:189716/251‑1 (MQ=255)                                                                                                                                                                                                                gcGTCTTCACCGGTGAGTTGGT‑CGAAGGCCTGTCTGGTCTGGCAGACAGTGATGCAA‑TGAACGCGTCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTTAACAGAGTCATTGAGCAAGCGCGTGCCGTCAAGAAAGTGGGTGGCTCGCGGGAAGCTGCAGGAACCTTGTATGCATAGCCGTAGCTGGCGGCGAAACTGACCGCGCTGGGCTATGAATTGCGATTTGTCTTGTGGACCTCCTGCGCt                                 <  2:291787/250‑1 (MQ=255)                                                                                                                                                                                                                aCGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgcgc                                  <  1:56455/250‑1 (MQ=255)                                                                                                                                                                                                                   tcttctCCGGTGAGG‑GGTACGAATGCCTGTTTGGTCTGGCAGACAGTGAATCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGTCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                               <  1:72996/250‑1 (MQ=255)                                                                                                                                                                                                                   ccTTCACCGGGTAGG‑GGTACGAAGGCTTGGTTTGTCTGGCAGACAGTGAAGCAA‑TGAACGGTGCGGTCTGGGACGAGCTGTTGAAAGTGCGGGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCGCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCTGCGCTAcc                              <  2:553044/250‑1 (MQ=255)                                                                                                                                                                                                                      gctccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                           <  2:226253/248‑1 (MQ=255)                                                                                                                                                                                                                        cccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGc                         <  1:19025/250‑1 (MQ=255)                                                                                                                                                                                                                         ccGGTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                           >  1:226253/1‑248 (MQ=255)                                                                                                                                                                                                                           ggTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACACAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGGAACTGACCGCGCTGGGGGATGAATTACGATTTGTCTTTTTGGCCGCCGGCGCTACCGtttgcg                       >  2:387631/1‑246 (MQ=255)                                                                                                                                                                                                                            gTGAGT‑GGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGAc                     >  1:90918/1‑251 (MQ=255)                                                                                                                                                                                                                              gAGT‑GGTACGAATGCCTGTTTTGTCTGGCAGACAGTGAATCAA‑TGATCGAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGAGTGCCGACAAGAAAGTGTGTGGCTCGCTGGAAGCGTCAGTACCCTTTTATGCAGATCCGGAACTGGCGGCGAAACTGACTGCGCTGGGCGATGAATTACGATGTGTCTTTTTGCCCTCCGGCGCTTCCGTTGCAGACta                   <  2:587058/251‑1 (MQ=255)                                                                                                                                                                                                                                             ccTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctg     <  1:164251/251‑1 (MQ=255)                                                                                                                                                                                                                                                gTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct   <  1:385727/250‑1 (MQ=255)                                                                                                                                                                                                                                                gTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct   <  1:151902/250‑1 (MQ=255)                                                                                                                                                                                                                                                 tttGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAGCCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGGTGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgctg  >  1:74582/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGT‑GGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTG  >  NC_000913/24538‑25025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑