Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,670	G→T	V760V (GTG→GTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,670	0	G	T	95.7%	234.9 / NA	94	V760V (GTG→GTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  major base T (44/44);  minor base A (4/0);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAG  >  NC_000913/24420‑24908                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 aaCCGGAAGAGATGGTGGTACTAGATCGCTGGGCCGTAGGATATGCGAAAGCGGCACAGGAAGACATCCTCACGGCGTAAGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTATGCTCCGTTGAGATGGGTTACTTCTCCCTCGACATCATCAAAGACCGACAGTCAACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGACAGACTGCGCTGTATCACATCGCAGACGCGCTGGTg                                                                                                                                                                                                                                                  >  2:112493/1‑251 (MQ=255)       aaGAGATGGTGGTACTGGATCGCTGGGAAGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTAGCACCTCGCAGAAGCGCTGGTTCGCTgg                                                                                                                                                                                                                                            >  2:436000/1‑251 (MQ=255)       aaGAGAAGGTGGAACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACCTCATCAAAGACCGTCAGTACACCGCCAAAGCGGAAAGAGTTGCGCGTCGTAGATGCCCGACTGCGCTGTATCACATCGCCGCAGCGCTGGTtcgcagg                                                                                                                                                                                                                                            >  2:631888/1‑248 (MQ=255)         gagaTGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGAt                                                                                                                                                                                                                                          >  1:422106/1‑251 (MQ=255)           gaTGGTGGTACTGGATCGCTGGGCCTGAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATgg                                                                                                                                                                                                                                        <  2:191121/251‑1 (MQ=255)            aTGGTGGTACTGGACCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCTTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGc                                                                                                                                                                                                                                       <  2:419518/251‑1 (MQ=255)              ggtgttcatgtgtagctggtacgttggTTGTGCGAAAGCGGCACAGGAAGACAGCCTCAAGGCGTTCGAAGCTTAAGATGTCCACGAAGTGGTCCAACGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGTCATCAGCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCTCGTCGTAGCTTCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGTATGGCa                                                                                                                                                                                                                                      <  1:655398/225‑1 (MQ=255)               gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCAcc                                                                                                                                                                                                                                    <  1:10809/251‑1 (MQ=255)                 ggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCa                                                                                                                                                                                                                                   <  1:575982/250‑1 (MQ=255)                          tCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCt                                                                                                                                                                                                                        >  1:367806/1‑251 (MQ=255)                            ggtGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTc                                                                                                                                                                                                                      <  2:40921/249‑1 (MQ=255)                            gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTc                                                                                                                                                                                                                      <  1:652321/251‑1 (MQ=255)                            gCTGGACCGTACGTTGTGAGTAAGCGGCACAGGAAGACATCCCCAACGCGAACGCAGCATAAGAATTCCACAAAGTGGCACACCGTCTGATGCGCTACTGCTCCGTTGAGATGGGTTCCTTATACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACCGCGTGGCGCGTGTTAGCTGCCAGACTGCGCTGTGTCACATCGAAGAAGCTCTGGCACGCTGGATGGCACCAATACTCC‑CCTTc                                                                                                                                                                                                                      >  2:452336/1‑251 (MQ=255)                               gggCCGTAGGTTTTGCGAAAGCGGCCCAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCGGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCa                                                                                                                                                                                                                     <  2:467860/249‑1 (MQ=255)                               gggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCa                                                                                                                                                                                                                     <  1:487878/249‑1 (MQ=255)                                  ccttAGGTGGTGCGAAAGCGGCACAGGAAGTCATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACGCCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCt                                                                                                                                                                                                                <  2:272192/248‑1 (MQ=255)                                  ccGTAGGTTGTGCGAAAGCGGCACAGGACGACATCCTCAAAGCGTACGAAGCATACGATTTCCACGAAGTGGAACAGCGTCTGGTGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACCCCGCCAAAGCGCCCCGCGTGGCGCGTCGTAGCTCCCATACTGCGCTGCATACCATCGCCGCAGAGCTGGTGCGCTGGATGGCACCAATCCTCC‑CCttacccact                                                                                                                                                                                                                >  2:498618/1‑244 (MQ=255)                                  ccGTAGGTGGGGCGAAAGCGGAACAGGAAGACATCCTCAAGGCGTACGAAGCATACGTTTTCCACGAAGTGGTACTGCGGCTGATGAGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACCCCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCt                                                                                                                                                                                                                <  2:287163/251‑1 (MQ=255)                                    gTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCtg                                                                                                                                                                                                               >  1:625349/1‑250 (MQ=255)                                      aGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCtgatg                                                                                                                                                                                                            >  1:305606/1‑251 (MQ=255)                                             gCGAAAGCGGCACAGGAAGCCATCCTAAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCCGAATGCGCTGTATAACATAGCAGAAGCGCTGGTTCGCCGGATGTCACCAAGCCTCC‑CCTTCACCCCTGGTGAAggggg                                                                                                                                                                                                     >  2:149640/1‑247 (MQ=255)                                               gagaGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTAGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTACTTCTACCTCGACATCATCAAAGACCGTCAGTCCACCACCAAACCGGACAGCGTGGCGCGTCGTAACTGCCCGACTGCGCTGTATCACATCGCAGTAGCGCTGGAGCGCTAGATGGCACCAAGCTACT‑CCTTCACCGCCGATGCAGGGTggg                                                                                                                                                                                                   >  2:152385/4‑251 (MQ=255)                                                aaaGCGGCACAGGAAGACATCCTCCAGGCGTGCGAAGAATACGATTTCCACGAAGTGGTACAGCGTCTGATGAGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGAGCTGCATCACATCGCAGAAGCGCTGGTTCGCCGGATGGCCCCAATCCTCT‑CCTTAACCGCAGATGAGGTGTgggg                                                                                                                                                                                                  >  2:670625/1‑251 (MQ=255)                                                aaaGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTgggg                                                                                                                                                                                                  >  1:366305/1‑251 (MQ=255)                                                  aGCGGCACAGGAAGACCTCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCt                                                                                                                                                                                                >  1:168713/1‑251 (MQ=255)                                                   gCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGAGTTCCACGAAGTGTTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCCCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTTCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTa                                                                                                                                                                                               <  1:620013/251‑1 (MQ=255)                                                      gCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCt                                                                                                                                                                                            >  1:424326/1‑251 (MQ=255)                                                       cccaGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCAGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTg                                                                                                                                                                                           <  1:345344/249‑1 (MQ=255)                                                        acaGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGCTGAAGTGTGGGGATACCTGc                                                                                                                                                                                          >  1:615425/1‑251 (MQ=255)                                                          aGGAAGACCTCCACAAGGCGTACGAAGAATACGATTTCCACGAAGTGGTACAGCGTCTGATGCTCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGAACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCg                                                                                                                                                                                        <  2:575909/251‑1 (MQ=255)                                                          aGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCg                                                                                                                                                                                        >  1:586418/1‑251 (MQ=255)                                                                   tCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATTGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGt                                                                                                                                                                               >  1:600778/1‑251 (MQ=255)                                                                       cAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGCACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGac                                                                                                                                                                            >  1:437745/1‑249 (MQ=255)                                                                        aaGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGaaa                                                                                                                                                                           >  1:502209/1‑250 (MQ=255)                                                                          ggCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAAt                                                                                                                                                                        >  1:660385/1‑251 (MQ=255)                                                                            cGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATAc                                                                                                                                                                      <  1:6997/251‑1 (MQ=255)                                                                                tgAAGCATACGATTTACACGAAGAGGTCCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTGCATCTACCTCGACCTCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGATCGCTGGATGGCACCCATCCTCT‑CCTTCCCCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGACCGTGaaccttaagtct                                                                                                                                                                  >  2:98543/2‑241 (MQ=255)                                                                                 gAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTCTCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAGGTGTGGGGCTCACAGCCGGGCGGACGTGAAAAATACGTCtt                                                                                                                                                                 >  2:450383/1‑251 (MQ=255)                                                                                    gCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGTACGTGAAAAATACGTCTTCAc                                                                                                                                                              >  2:446061/1‑251 (MQ=255)                                                                                         cGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTg                                                                                                                                                         >  1:457543/1‑251 (MQ=255)                                                                                           attttcactaagtgttccaacGTCTGATGCGCTTTTGCGCCGTTGATATGGGTTCCTTCTACCTCGACATCACCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAg                                                                                                                                                       <  2:509168/231‑1 (MQ=255)                                                                                                     aGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACAGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGACGCGCTGGATCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGCGTGGTACGa                                                                                                                                               >  1:657817/1‑249 (MQ=255)                                                                                                       tGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGc                                                                                                                                           >  1:553044/1‑251 (MQ=255)                                                                                                             ggCGTCTGATGGGCTGTTGCCCTGTTGGGATGGGTTACTTCTACCGCGACATCCTCAAATACCGGCAGTACTCTGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGttt                                                                                                                                     <  2:555585/250‑1 (MQ=255)                                                                                                             aGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGttt                                                                                                                                     <  1:436000/251‑1 (MQ=255)                                                                                                                gTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTggt                                                                                                                                  <  1:520612/251‑2 (MQ=255)                                                                                                                gTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTggt                                                                                                                                  >  1:89361/1‑250 (MQ=255)                                                                                                                       gcgcTTCTGCCCCGTAGGTATGGGTTCCTCCTACCTCGACATCATCAAAGCCCGCCAGTCCACCTCCAAAGCGGACAGCGTGGCGCGGCGTAGCTGCCAGACTGCGCTGTAGCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGGCTGGCAg                                                                                                                           <  2:660385/251‑1 (MQ=255)                                                                                                                        cgcTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGa                                                                                                                          <  1:149640/251‑1 (MQ=255)                                                                                                                                  ccGTTGAGATGGGTTCCTTCTCCCTCGACACCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGGGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                                                                <  2:305606/251‑2 (MQ=255)                                                                                                                                   cGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                                                                >  1:485911/1‑249 (MQ=255)                                                                                                                                    gTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGCACGTCAGTACAACGCCAAAGCGGACAGCGTGCCGCGTTGTAGCTGCCAGACCGCGCTCTATCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTACTGAAGTGTGGGGCTACCTGCCGGGCGACCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGTCTGGCAGACAGTGAAGcatt                                                                                                              >  1:286356/1‑248 (MQ=255)                                                                                                                                      tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑Tg                                                                                                            >  2:618071/1‑250 (MQ=255)                                                                                                                                        agaTGGGTTCCTTCTACCTCGACATCATCAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCCCAGAAGCGCGGGGTCGCTGGAAGGCACCAATCCTCT‑CCTTCACCGCTGATGTAGTGTGGGGCTACCTGCCGGTCGAGCGTGAAAAATACGTCTTCACCGGGGAGTGGTCAGAAGGACTGTTTGTTCTGGCAGACAGTgcaacaatgaac                                                                                                          >  2:539698/1‑240 (MQ=255)                                                                                                                                        agaTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAc                                                                                                         <  1:651974/251‑1 (MQ=255)                                                                                                                                        aactGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGAGCGTCGTAGCTGCCAGACTGCGCTGGATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTTGCAGACCGTGAAGCAA‑TGAAc                                                                                                         <  2:444006/248‑1 (MQ=255)                                                                                                                                              gTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGGGTGGGGCTACCTGCCGGGCGCACGTGAAAAATACGTCTTCACCGGTGGGGGGTACGAAGGCCGGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAcaatacg                                                                                                   >  2:19025/1‑245 (MQ=255)                                                                                                                                               ttCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGTGGTACGCAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGt                                                                                                  >  1:587058/1‑251 (MQ=255)                                                                                                                                                     cTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTggg                                                                                            >  1:433730/1‑251 (MQ=255)                                                                                                                                                              aattaccaAAGACCGACAGTACACTGCCAAAGCGGACAGCGTGGATCGTCGTAGCTGCAAGACTGCGCTGTATCCCATCGCAGAATCGCTGGTTCGCTGGATGGCACCATTCCGCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTGGGGCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGt                                                                                   <  2:386448/245‑1 (MQ=255)                                                                                                                                                                 catcaAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTg                                                                                 >  1:55189/1‑250 (MQ=255)                                                                                                                                                                    caAAGCCCGTCAGAACCCCGCCAAAGCGGACAGCGTGGCGCTTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGaa                                                                               <  2:433730/249‑1 (MQ=255)                                                                                                                                                                    caAAGACCGGCAGAACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAGCTGCCAGAGTGCGCTGTATAACATCGCAGCAGCGCTGGCACGCTGGAGGGCACTAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTGCATGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAG‑GACAGAACGATGCGTTCGGGGACGATGTGTTGac                                                                               >  2:51373/1‑248 (MQ=255)                                                                                                                                                                    aaaaGACCGTCCGTACTCCGCCAAAGCGGACAGCGGGGCGCGGCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAg                                                                             <  2:327459/250‑1 (MQ=255)                                                                                                                                                                      aaGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGACCGTGTAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGGAAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTg                                                                           >  2:201536/1‑251 (MQ=255)                                                                                                                                                                       agtccGTCAGTACACCGCCAAGGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGc                                                                          <  1:452336/248‑1 (MQ=255)                                                                                                                                                                        gACCGGCAGTCCCCCGCCAAATCGGTCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCg                                                                         <  2:502209/251‑1 (MQ=255)                                                                                                                                                                          ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                                       >  1:512676/1‑251 (MQ=255)                                                                                                                                                                           cGTCAGTACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGACCGATGAGTTCTGGGCCGAGCTGTTGAAAGCGCGTgg                                                                      >  2:90250/1‑251 (MQ=255)                                                                                                                                                                             gcagaccccccacAAAGCGGCCAGCGTGGCGCGTAGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGGTTGATGGCACCAATCCGCC‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                                                                      <  2:17430/237‑1 (MQ=255)                                                                                                                                                                              cAGTACACCGCCAAAGCGTACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCTTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                                                    <  1:440364/250‑1 (MQ=255)                                                                                                                                                                               aGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGa                                                                   >  1:120741/1‑250 (MQ=255)                                                                                                                                                                                  accccGCCAAAGCGCACAGCGTGGCGCGCCGTAGCTGCCAGACTGCGCTGTATCACATCGCATAAGCGCTGGTTCGCTGGATGGCTCCAA‑CCCCTGCCTTCACCGCTGATGAAGTGTGGGGCCACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCGGGGACGAGCTGTTGAAAGGGCGTGGCGAAGt                                                                <  2:55189/247‑1 (MQ=255)                                                                                                                                                                                   cacCGCCAAAGCGGACAGGGGGGCGGGGCGTCGCTGCACGACTGCGCTGTATCCAATCGCAGAAGCGCTGGGTCGCGGGATGGCACCAATCTTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTGGAAAGTGCGTGGCGAAGTGa                                                              <  2:110517/251‑1 (MQ=255)                                                                                                                                                                                    cccGCCAAAGCGGCCTGCGGGTCGCGTCGTAGCTGCCAGACTGCGCTTTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa                                                             <  2:73324/250‑1 (MQ=255)                                                                                                                                                                                    cccGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCATACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa                                                             <  1:389554/250‑1 (MQ=255)                                                                                                                                                                                           aaGCGGACAGCGTAGCGAGACGTAGCTGCCAGACTGAGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGAACAAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGTGGAGAAGTGACCACAGTc                                                      >  2:449085/1‑251 (MQ=255)                                                                                                                                                                                                gACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                                                  >  1:296392/1‑250 (MQ=255)                                                                                                                                                                                                 aCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGTCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGAGGCGAAGTGGACAAAGTCATTg                                                  >  2:554332/1‑249 (MQ=255)                                                                                                                                                                                                 aCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAg                                                <  1:432931/251‑1 (MQ=255)                                                                                                                                                                                                       tgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcg                                          >  1:115240/1‑251 (MQ=255)                                                                                                                                                                                                           gcgTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTg                                      <  1:246611/251‑1 (MQ=255)                                                                                                                                                                                                               cgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTAAGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGGGCCg                                   >  1:149773/1‑250 (MQ=255)                                                                                                                                                                                                                gtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGCTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGAc                                 >  1:106661/1‑251 (MQ=255)                                                                                                                                                                                                                      gCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                           >  2:344857/1‑235 (MQ=255)                                                                                                                                                                                                                      gCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                           <  1:344857/235‑1 (MQ=255)                                                                                                                                                                                                                         aGACTGCGCTGTAACACATCGCAGAAGCGATGGATCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGATGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGCGCAAACGCGGGCCGACAAGAACgtg                        >  2:669859/1‑251 (MQ=255)                                                                                                                                                                                                                                   gtatCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGc              >  1:488934/2‑251 (MQ=255)                                                                                                                                                                                                                                   gtatCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGc              >  1:461197/2‑251 (MQ=255)                                                                                                                                                                                                                                     atCACATCGCAGAAGCGCTGGTTCGCTTGATGGCACCAATCCTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCtt            <  1:289215/251‑2 (MQ=255)                                                                                                                                                                                                                                     atCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTg            <  1:289189/251‑1 (MQ=255)                                                                                                                                                                                                                                         catggCAGAAGCGATGGTTCTCTGGATGGCACCAATCCTCG‑TCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAg        <  2:626154/247‑1 (MQ=255)                                                                                                                                                                                                                                          aTCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGc       >  2:164251/1‑251 (MQ=255)                                                                                                                                                                                                                                            cGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCgg     <  1:450383/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAGAAGCGCTGGTTCGCTGGATGGCTCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCa   <  2:89361/251‑1 (MQ=255)                                                                                                                                                                                                                                               aGAAGCGCTGGGTCGCTGGATGGCATCAATCCACG‑CCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAg  <  2:168713/251‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 AACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAG  >  NC_000913/24420‑24908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑