Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,646	A→G	L752L (CTA→CTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,646	0	A	G	97.9%	294.7 / NA	96	L752L (CTA→CTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (53/40);  minor base T (1/1);  total (55/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTG  >  NC_000913/24399‑24886                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   aTCCAGCAAAAGATATGAGGAAACCGGAAGAGATGGTGGTACTGGATCGCAGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGGGGACCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTAGCCCTCATCAAAGACCGTCAGTTCACCGCCAAAGCGTCAAGCGGGGCGCTGCGTAG‑CTGCCAGACTGCGCTgtat                                                                                                                                                                                                                                                 >  2:289189/1‑251 (MQ=255)   ccAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATc                                                                                                                                                                                                                                                >  1:291787/1‑250 (MQ=255)       aaaaaGATATGGTGAAACCGGAAGAGATGGTGGTACTGTATCGCTGGGCCGTAGGTTGTGGGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATc                                                                                                                                                                                                                                           <  2:119093/250‑1 (MQ=255)            gATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGc                                                                                                                                                                                                                                         >  1:115013/1‑248 (MQ=255)               atGGGGAAACCGGAGGAGATGAGGGTACTGGCTCGCTGGGACGTCGGATGAGCGAAAGCGGCACCGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGACGTGGTAAAGCGTCTGCTGCGCTTCTGCTCCGTTGAGATGGGTTCATTCTACATCGACATCATCAAAGACCGTCAGTAAACCGCCAAATCGGACATCGTGTCGAGTCGTAC‑CTGCCAGAGTGCGCTTTATccaatctccgaaac                                                                                                                                                                                                                                   >  2:151902/1‑238 (MQ=255)                  gTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGACAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGCCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAgcgcc                                                                                                                                                                                                                                >  2:189089/1‑250 (MQ=255)                    gAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTgg                                                                                                                                                                                                                              >  1:73324/1‑251 (MQ=255)                    gAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTg                                                                                                                                                                                                                               <  1:165054/250‑1 (MQ=255)                      aaCCGGAAGAGATGGTGGTACTAGATCGCTGGGCCGTAGGATATGCGAAAGCGGCACAGGAAGACATCCTCACGGCGTAAGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTATGCTCCGTTGAGATGGGTTACTTCTCCCTCGACATCATCAAAGACCGACAGTCAACCGCCAACGCGGACAGCGTGGCGCGTCGTAG‑CTGACAGACTGCGCTGTATCACATCGCAGACGCGCTGGTg                                                                                                                                                                                                                            >  2:112493/1‑251 (MQ=255)                      aaCCGGAAGAGATGGAGGTACTGGATCGCTGGGCAGTAGGTTGTGCGAAAGCGGAACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACATCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCTGACAGCGTGGAGCGTCGTAGATTGCAAG‑CTGCGCTGTATCCCATCGCAGAAGAGCTGGtt                                                                                                                                                                                                                            >  2:56455/1‑250 (MQ=255)                            aaGAGATGGTGGTACTGGATCGCTGGGAAGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTAGCACCTCGCAGAAGCGCTGGTTCGCTgg                                                                                                                                                                                                                      >  2:436000/1‑251 (MQ=255)                            aaGAGAAGGTGGAACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACCTCATCAAAGACCGTCAGTACACCGCCAAAGCGGAAAGAGTTGCGCGTCGTAG‑ATGCCCGACTGCGCTGTATCACATCGCCGCAGCGCTGGTtcgcagg                                                                                                                                                                                                                      >  2:631888/1‑248 (MQ=255)                              gagaTGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGAt                                                                                                                                                                                                                    >  1:422106/1‑251 (MQ=255)                                gacggtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCAAAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTGCTGCTTCGTAGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATca                                                                                                                                                                                                                                               <  1:126859/219‑1 (MQ=255)                                gaTGGTGGTACTGGATCGCTGGGCCTGAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATgg                                                                                                                                                                                                                  <  2:191121/251‑1 (MQ=255)                                 aTGGTGGTACTGGACCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCTTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGc                                                                                                                                                                                                                 <  2:419518/251‑1 (MQ=255)                                   ggtgttcatgtgtagctggtacgttggTTGTGCGAAAGCGGCACAGGAAGACAGCCTCAAGGCGTTCGAAGCTTAAGATGTCCACGAAGTGGTCCAACGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGTCATCAGCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCTCGTCGTAG‑CTTCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGTATGGCa                                                                                                                                                                                                                <  1:655398/225‑1 (MQ=255)                                    gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCAcc                                                                                                                                                                                                              <  1:10809/251‑1 (MQ=255)                                      ggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCa                                                                                                                                                                                                             <  1:575982/250‑1 (MQ=255)                                               tCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCt                                                                                                                                                                                                  >  1:367806/1‑251 (MQ=255)                                                 ggtGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTc                                                                                                                                                                                                <  2:40921/249‑1 (MQ=255)                                                 gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTc                                                                                                                                                                                                <  1:652321/251‑1 (MQ=255)                                                 gCTGGACCGTACGTTGTGAGTAAGCGGCACAGGAAGACATCCCCAACGCGAACGCAGCATAAGAATTCCACAAAGTGGCACACCGTCTGATGCGCTACTGCTCCGTTGAGATGGGTTCCTTATACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACCGCGTGGCGCGTGTTAG‑CTGCCAGACTGCGCTGTGTCACATCGAAGAAGCTCTGGCACGCTGGATGGCACCAATACTCC‑CCTTc                                                                                                                                                                                                >  2:452336/1‑251 (MQ=255)                                                    gggCCGTAGGTTTTGCGAAAGCGGCCCAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCGGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCa                                                                                                                                                                                               <  2:467860/249‑1 (MQ=255)                                                    gggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCa                                                                                                                                                                                               <  1:487878/249‑1 (MQ=255)                                                       ccttAGGTGGTGCGAAAGCGGCACAGGAAGTCATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACGCCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCt                                                                                                                                                                                          <  2:272192/248‑1 (MQ=255)                                                       ccGTAGGTTGTGCGAAAGCGGCACAGGACGACATCCTCAAAGCGTACGAAGCATACGATTTCCACGAAGTGGAACAGCGTCTGGTGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACCCCGCCAAAGCGCCCCGCGTGGCGCGTCGTAG‑CTCCCATACTGCGCTGCATACCATCGCCGCAGAGCTGGTGCGCTGGATGGCACCAATCCTCC‑CCttacccact                                                                                                                                                                                          >  2:498618/1‑244 (MQ=255)                                                       ccGTAGGTGGGGCGAAAGCGGAACAGGAAGACATCCTCAAGGCGTACGAAGCATACGTTTTCCACGAAGTGGTACTGCGGCTGATGAGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACCCCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCt                                                                                                                                                                                          <  2:287163/251‑1 (MQ=255)                                                         gTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCtg                                                                                                                                                                                         >  1:625349/1‑250 (MQ=255)                                                           aGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCtgatg                                                                                                                                                                                      >  1:305606/1‑251 (MQ=255)                                                                  gCGAAAGCGGCACAGGAAGCCATCCTAAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCCGAATGCGCTGTATAACATAGCAGAAGCGCTGGTTCGCCGGATGTCACCAAGCCTCC‑CCTTCACCCCTGGTGAAggggg                                                                                                                                                                               >  2:149640/1‑247 (MQ=255)                                                                    gagaGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTAGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTACTTCTACCTCGACATCATCAAAGACCGTCAGTCCACCACCAAACCGGACAGCGTGGCGCGTCGTAA‑CTGCCCGACTGCGCTGTATCACATCGCAGTAGCGCTGGAGCGCTAGATGGCACCAAGCTACT‑CCTTCACCGCCGATGCAGGGTggg                                                                                                                                                                             >  2:152385/4‑251 (MQ=255)                                                                     aaaGCGGCACAGGAAGACATCCTCCAGGCGTGCGAAGAATACGATTTCCACGAAGTGGTACAGCGTCTGATGAGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGAGCTGCATCACATCGCAGAAGCGCTGGTTCGCCGGATGGCCCCAATCCTCT‑CCTTAACCGCAGATGAGGTGTgggg                                                                                                                                                                            >  2:670625/1‑251 (MQ=255)                                                                     aaaGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTgggg                                                                                                                                                                            >  1:366305/1‑251 (MQ=255)                                                                       aGCGGCACAGGAAGACCTCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCt                                                                                                                                                                          >  1:168713/1‑251 (MQ=255)                                                                        gCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGAGTTCCACGAAGTGTTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCCCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTTCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTa                                                                                                                                                                         <  1:620013/251‑1 (MQ=255)                                                                           gCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCt                                                                                                                                                                      >  1:424326/1‑251 (MQ=255)                                                                            cccaGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCAGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTg                                                                                                                                                                     <  1:345344/249‑1 (MQ=255)                                                                             acaGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGCTGAAGTGTGGGGATACCTGc                                                                                                                                                                    >  1:615425/1‑251 (MQ=255)                                                                               aGGAAGACCTCCACAAGGCGTACGAAGAATACGATTTCCACGAAGTGGTACAGCGTCTGATGCTCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGAACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCg                                                                                                                                                                  <  2:575909/251‑1 (MQ=255)                                                                               aGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCg                                                                                                                                                                  >  1:586418/1‑251 (MQ=255)                                                                                        tCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATTGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGt                                                                                                                                                         >  1:600778/1‑251 (MQ=255)                                                                                            cAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGCACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGac                                                                                                                                                      >  1:437745/1‑249 (MQ=255)                                                                                             aaGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGaaa                                                                                                                                                     >  1:502209/1‑250 (MQ=255)                                                                                               ggCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAAt                                                                                                                                                  >  1:660385/1‑251 (MQ=255)                                                                                                 cGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATAc                                                                                                                                                <  1:6997/251‑1 (MQ=255)                                                                                                     tgAAGCATACGATTTACACGAAGAGGTCCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTGCATCTACCTCGACCTCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGATCGCTGGATGGCACCCATCCTCT‑CCTTCCCCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGACCGTGaaccttaagtct                                                                                                                                            >  2:98543/2‑241 (MQ=255)                                                                                                      gAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTCTCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAGGTGTGGGGCTCACAGCCGGGCGGACGTGAAAAATACGTCtt                                                                                                                                           >  2:450383/1‑251 (MQ=255)                                                                                                         gCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGTACGTGAAAAATACGTCTTCAc                                                                                                                                        >  2:446061/1‑251 (MQ=255)                                                                                                              cGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTg                                                                                                                                   >  1:457543/1‑251 (MQ=255)                                                                                                                attttcactaagtgttccaacGTCTGATGCGCTTTTGCGCCGTTGATATGGGTTCCTTCTACCTCGACATCACCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAg                                                                                                                                 <  2:509168/231‑1 (MQ=255)                                                                                                                          aGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACAGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGACGCGCTGGATCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGCGTGGTACGa                                                                                                                         >  1:657817/1‑249 (MQ=255)                                                                                                                            tGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGc                                                                                                                     >  1:553044/1‑251 (MQ=255)                                                                                                                                  ggCGTCTGATGGGCTGTTGCCCTGTTGGGATGGGTTACTTCTACCGCGACATCCTCAAATACCGGCAGTACTCTGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGttt                                                                                                               <  2:555585/250‑1 (MQ=255)                                                                                                                                  aGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGttt                                                                                                               <  1:436000/251‑1 (MQ=255)                                                                                                                                     gTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTggt                                                                                                            <  1:520612/251‑2 (MQ=255)                                                                                                                                     gTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTggt                                                                                                            >  1:89361/1‑250 (MQ=255)                                                                                                                                            gcgcTTCTGCCCCGTAGGTATGGGTTCCTCCTACCTCGACATCATCAAAGCCCGCCAGTCCACCTCCAAAGCGGACAGCGTGGCGCGGCGTAG‑CTGCCAGACTGCGCTGTAGCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGGCTGGCAg                                                                                                     <  2:660385/251‑1 (MQ=255)                                                                                                                                             cgcTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGa                                                                                                    <  1:149640/251‑1 (MQ=255)                                                                                                                                                       ccGTTGAGATGGGTTCCTTCTCCCTCGACACCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGGGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                                          <  2:305606/251‑2 (MQ=255)                                                                                                                                                        cGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                                          >  1:485911/1‑249 (MQ=255)                                                                                                                                                         gTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGCACGTCAGTACAACGCCAAAGCGGACAGCGTGCCGCGTTGTAG‑CTGCCAGACCGCGCTCTATCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTACTGAAGTGTGGGGCTACCTGCCGGGCGACCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGTCTGGCAGACAGTGAAGcatt                                                                                        >  1:286356/1‑248 (MQ=255)                                                                                                                                                           tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑Tg                                                                                      >  2:618071/1‑250 (MQ=255)                                                                                                                                                             agaTGGGTTCCTTCTACCTCGACATCATCAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCCCAGAAGCGCGGGGTCGCTGGAAGGCACCAATCCTCT‑CCTTCACCGCTGATGTAGTGTGGGGCTACCTGCCGGTCGAGCGTGAAAAATACGTCTTCACCGGGGAGTGGTCAGAAGGACTGTTTGTTCTGGCAGACAGTgcaacaatgaac                                                                                    >  2:539698/1‑240 (MQ=255)                                                                                                                                                             agaTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAc                                                                                   <  1:651974/251‑1 (MQ=255)                                                                                                                                                             aactGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGAGCGTCGTAG‑CTGCCAGACTGCGCTGGATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTTGCAGACCGTGAAGCAA‑TGAAc                                                                                   <  2:444006/248‑1 (MQ=255)                                                                                                                                                                   gTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGGGTGGGGCTACCTGCCGGGCGCACGTGAAAAATACGTCTTCACCGGTGGGGGGTACGAAGGCCGGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAcaatacg                                                                             >  2:19025/1‑245 (MQ=255)                                                                                                                                                                    ttCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGTGGTACGCAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGt                                                                            >  1:587058/1‑251 (MQ=255)                                                                                                                                                                          cTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTggg                                                                      >  1:433730/1‑251 (MQ=255)                                                                                                                                                                                   aattaccaAAGACCGACAGTACACTGCCAAAGCGGACAGCGTGGATCGTCGTAG‑CTGCAAGACTGCGCTGTATCCCATCGCAGAATCGCTGGTTCGCTGGATGGCACCATTCCGCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTGGGGCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGt                                                             <  2:386448/245‑1 (MQ=255)                                                                                                                                                                                      catcaAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTg                                                           >  1:55189/1‑250 (MQ=255)                                                                                                                                                                                         caAAGCCCGTCAGAACCCCGCCAAAGCGGACAGCGTGGCGCTTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGaa                                                         <  2:433730/249‑1 (MQ=255)                                                                                                                                                                                         caAAGACCGGCAGAACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAG‑CTGCCAGAGTGCGCTGTATAACATCGCAGCAGCGCTGGCACGCTGGAGGGCACTAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTGCATGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAG‑GACAGAACGATGCGTTCGGGGACGATGTGTTGac                                                         >  2:51373/1‑248 (MQ=255)                                                                                                                                                                                         aaaaGACCGTCCGTACTCCGCCAAAGCGGACAGCGGGGCGCGGCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAg                                                       <  2:327459/250‑1 (MQ=255)                                                                                                                                                                                           aaGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGACCGTGTAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGGAAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTg                                                     >  2:201536/1‑251 (MQ=255)                                                                                                                                                                                            agtccGTCAGTACACCGCCAAGGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGc                                                    <  1:452336/248‑1 (MQ=255)                                                                                                                                                                                             gACCGGCAGTCCCCCGCCAAATCGGTCAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCg                                                   <  2:502209/251‑1 (MQ=255)                                                                                                                                                                                               ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                 >  1:512676/1‑251 (MQ=255)                                                                                                                                                                                                cGTCAGTACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGACCGATGAGTTCTGGGCCGAGCTGTTGAAAGCGCGTgg                                                >  2:90250/1‑251 (MQ=255)                                                                                                                                                                                                  gcagaccccccacAAAGCGGCCAGCGTGGCGCGTAGTAG‑CTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGGTTGATGGCACCAATCCGCC‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                                                <  2:17430/237‑1 (MQ=255)                                                                                                                                                                                                   cAGTACACCGCCAAAGCGTACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCTTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                              <  1:440364/250‑1 (MQ=255)                                                                                                                                                                                                    aGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGa                                             >  1:120741/1‑250 (MQ=255)                                                                                                                                                                                                       accccGCCAAAGCGCACAGCGTGGCGCGCCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCATAAGCGCTGGTTCGCTGGATGGCTCCAA‑CCCCTGCCTTCACCGCTGATGAAGTGTGGGGCCACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCGGGGACGAGCTGTTGAAAGGGCGTGGCGAAGt                                          <  2:55189/247‑1 (MQ=255)                                                                                                                                                                                                        cacCGCCAAAGCGGACAGGGGGGCGGGGCGTCG‑CTGCACGACTGCGCTGTATCCAATCGCAGAAGCGCTGGGTCGCGGGATGGCACCAATCTTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTGGAAAGTGCGTGGCGAAGTGa                                        <  2:110517/251‑1 (MQ=255)                                                                                                                                                                                                         cccGCCAAAGCGGCCTGCGGGTCGCGTCGTAG‑CTGCCAGACTGCGCTTTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa                                       <  2:73324/250‑1 (MQ=255)                                                                                                                                                                                                         cccGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCATACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa                                       <  1:389554/250‑1 (MQ=255)                                                                                                                                                                                                                aaGCGGACAGCGTAGCGAGACGTAG‑CTGCCAGACTGAGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGAACAAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGTGGAGAAGTGACCACAGTc                                >  2:449085/1‑251 (MQ=255)                                                                                                                                                                                                                     gACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                            >  1:296392/1‑250 (MQ=255)                                                                                                                                                                                                                      aCAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGTCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGAGGCGAAGTGGACAAAGTCATTg                            >  2:554332/1‑249 (MQ=255)                                                                                                                                                                                                                      aCAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAg                          <  1:432931/251‑1 (MQ=255)                                                                                                                                                                                                                            tgGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcg                    >  1:115240/1‑251 (MQ=255)                                                                                                                                                                                                                                gcgTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTg                <  1:246611/251‑1 (MQ=255)                                                                                                                                                                                                                                    cgtAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTAAGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGGGCCg             >  1:149773/1‑250 (MQ=255)                                                                                                                                                                                                                                     gtAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGCTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGAc           >  1:106661/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                     >  2:344857/1‑235 (MQ=255)                                                                                                                                                                                                                                            gCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                     <  1:344857/235‑1 (MQ=255)                                                                                                                                                                                                                                               aGACTGCGCTGTAACACATCGCAGAAGCGATGGATCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGATGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGCGCAAACGCGGGCCGACAAGAACgtg  >  2:669859/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   ATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTG  >  NC_000913/24399‑24886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑