BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,616	T→C	S742S (AGT→AGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,616	0	T	C	97.1%	317.1 / NA	106	S742S (AGT→AGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (56/46); minor base A (3/0); total (59/47)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 106 total aligned reads displayed.

CCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAG  >  NC_000913/24373‑24862

   gctgGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGcgcg                                                                                                                                                                                                                                                   <  1:497875/249‑1 (MQ=255)

   gctgGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGcgt                                                                                                                                                                                                                                                  <  1:622574/250‑1 (MQ=255)

    ctgGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGATGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGcgtcg                                                                                                                                                                                                                                                >  1:99584/1‑251 (MQ=255)

      ggCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGGACTGGATCGCTGGGCAGTAGGGTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCAACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGGCAGTACACCGCCAAAGCGGACAGCGTGGCGcgtcgt                                                                                                                                                                                                                                               >  1:52003/1‑250 (MQ=255)

      ggCAAACCTGAAAGGTGTTGATCTAGCAAAAGATATTGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTa                                                                                                                                                                                                                                              <  2:239425/251‑1 (MQ=255)

        cAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGCGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGGGGTAAAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCACCAAAGCGGACAACGTGGCGCGACGTAG‑c                                                                                                                                                                                                                                           >  2:537549/1‑251 (MQ=255)

          aaCCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTg                                                                                                                                                                                                                                         <  1:544916/251‑1 (MQ=255)

          aaCCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGGGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACATGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTg                                                                                                                                                                                                                                         <  2:143256/251‑1 (MQ=255)

               gAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGcc                                                                                                                                                                                                                                       >  1:4112/1‑248 (MQ=255)

                     ttttGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTgcgc                                                                                                                                                                                                                              >  1:327459/1‑251 (MQ=255)

                     ttttGAACCAGCAAAAGATATAGTGAAACCGGACGAGAAGGTGGTATTGGATCGCTGAGCCGTAGGTTGAGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGGACGACGCATCCGATTTCCACGAGGAGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGATTCCTTCAACCTCGACATCATAAAAGACCGTCGGTCCACAGCCAAGGCGGAAGGCGGGGCGCGGCGTAG‑ATGCCAGACTgccc                                                                                                                                                                                                                              >  2:448916/1‑249 (MQ=255)

                      tttGATCCAGCAAAAGATATGGTGAAACTGGAAGAGATGGGGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTgcgc                                                                                                                                                                                                                              <  2:271303/250‑1 (MQ=255)

                          aTCCAGCCAAGGATATGGAGAAACCGGAAGATATGCTGTTACAGGAGCGATAGGCAGTAGGTTGAGCGAACGAGGCACAGGGAGACCACCTCAAGGCATACAAAGCCTACGATTTCCACGAAGTGGTACAGCATCTGATGCGATTCTGCTCCTATGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTACGTACAAACCCAAAGCGGACAGCGTGGGGCGTCGTAA‑CTGCAAGACAgcgttgact                                                                                                                                                                                                                         >  2:289215/1‑245 (MQ=255)

                          aTCCAGCAAAGGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCTCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGATATCCTCAAGGCGTACGCAGCATACTATTCCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATTGGTTCCTTCTACCTCGACATCATTAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCtgta                                                                                                                                                                                                                          <  2:504493/250‑4 (MQ=255)

                          aTCCAGCAAAAGATATGAGGAAACCGGAAGAGATGGTGGTACTGGATCGCAGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGGGGACCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTAGCCCTCATCAAAGACCGTCAGTTCACCGCCAAAGCGTCAAGCGGGGCGCTGCGTAG‑CTGCCAGACTGCGCTgtat                                                                                                                                                                                                                         >  2:289189/1‑251 (MQ=255)

                            ccAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATc                                                                                                                                                                                                                        >  1:291787/1‑250 (MQ=255)

                                aaaaaGATATGGTGAAACCGGAAGAGATGGTGGTACTGTATCGCTGGGCCGTAGGTTGTGGGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATc                                                                                                                                                                                                                   <  2:119093/250‑1 (MQ=255)

                                     gATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGc                                                                                                                                                                                                                 >  1:115013/1‑248 (MQ=255)

                                        atGGGGAAACCGGAGGAGATGAGGGTACTGGCTCGCTGGGACGTCGGATGAGCGAAAGCGGCACCGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGACGTGGTAAAGCGTCTGCTGCGCTTCTGCTCCGTTGAGATGGGTTCATTCTACATCGACATCATCAAAGACCGTCAGTAAACCGCCAAATCGGACATCGTGTCGAGTCGTAC‑CTGCCAGAGTGCGCTTTATccaatctccgaaac                                                                                                                                                                                                           >  2:151902/1‑238 (MQ=255)

                                           gTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGACAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGCCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAgcgcc                                                                                                                                                                                                        >  2:189089/1‑250 (MQ=255)

                                             gAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTgg                                                                                                                                                                                                      >  1:73324/1‑251 (MQ=255)

                                             gAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTg                                                                                                                                                                                                       <  1:165054/250‑1 (MQ=255)

                                               aaCCGGAAGAGATGGTGGTACTAGATCGCTGGGCCGTAGGATATGCGAAAGCGGCACAGGAAGACATCCTCACGGCGTAAGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTATGCTCCGTTGAGATGGGTTACTTCTCCCTCGACATCATCAAAGACCGACAGTCAACCGCCAACGCGGACAGCGTGGCGCGTCGTAG‑CTGACAGACTGCGCTGTATCACATCGCAGACGCGCTGGTg                                                                                                                                                                                                    >  2:112493/1‑251 (MQ=255)

                                               aaCCGGAAGAGATGGAGGTACTGGATCGCTGGGCAGTAGGTTGTGCGAAAGCGGAACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACATCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCTGACAGCGTGGAGCGTCGTAGATTGCAAG‑CTGCGCTGTATCCCATCGCAGAAGAGCTGGtt                                                                                                                                                                                                    >  2:56455/1‑250 (MQ=255)

                                                     aaGAGATGGTGGTACTGGATCGCTGGGAAGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTAGCACCTCGCAGAAGCGCTGGTTCGCTgg                                                                                                                                                                                              >  2:436000/1‑251 (MQ=255)

                                                     aaGAGAAGGTGGAACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACCTCATCAAAGACCGTCAGTACACCGCCAAAGCGGAAAGAGTTGCGCGTCGTAG‑ATGCCCGACTGCGCTGTATCACATCGCCGCAGCGCTGGTtcgcagg                                                                                                                                                                                              >  2:631888/1‑248 (MQ=255)

                                                       gagaTGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGAt                                                                                                                                                                                            >  1:422106/1‑251 (MQ=255)

                                                         gacggtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCAAAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTGCTGCTTCGTAGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATca                                                                                                                                                                                                                       <  1:126859/219‑1 (MQ=255)

                                                         gaTGGTGGTACTGGATCGCTGGGCCTGAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATgg                                                                                                                                                                                          <  2:191121/251‑1 (MQ=255)

                                                          aTGGTGGTACTGGACCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCTTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGc                                                                                                                                                                                         <  2:419518/251‑1 (MQ=255)

                                                            ggtgttcatgtgtagctggtacgttggTTGTGCGAAAGCGGCACAGGAAGACAGCCTCAAGGCGTTCGAAGCTTAAGATGTCCACGAAGTGGTCCAACGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGTCATCAGCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCTCGTCGTAG‑CTTCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGTATGGCa                                                                                                                                                                                        <  1:655398/225‑1 (MQ=255)

                                                             gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCAcc                                                                                                                                                                                      <  1:10809/251‑1 (MQ=255)

                                                               ggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCa                                                                                                                                                                                     <  1:575982/250‑1 (MQ=255)

                                                                        tCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCt                                                                                                                                                                          >  1:367806/1‑251 (MQ=255)

                                                                          ggtGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTc                                                                                                                                                                        <  2:40921/249‑1 (MQ=255)

                                                                          gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTc                                                                                                                                                                        <  1:652321/251‑1 (MQ=255)

                                                                          gCTGGACCGTACGTTGTGAGTAAGCGGCACAGGAAGACATCCCCAACGCGAACGCAGCATAAGAATTCCACAAAGTGGCACACCGTCTGATGCGCTACTGCTCCGTTGAGATGGGTTCCTTATACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACCGCGTGGCGCGTGTTAG‑CTGCCAGACTGCGCTGTGTCACATCGAAGAAGCTCTGGCACGCTGGATGGCACCAATACTCC‑CCTTc                                                                                                                                                                        >  2:452336/1‑251 (MQ=255)

                                                                             gggCCGTAGGTTTTGCGAAAGCGGCCCAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCGGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCa                                                                                                                                                                       <  2:467860/249‑1 (MQ=255)

                                                                             gggCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCa                                                                                                                                                                       <  1:487878/249‑1 (MQ=255)

                                                                                ccttAGGTGGTGCGAAAGCGGCACAGGAAGTCATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACGCCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCt                                                                                                                                                                  <  2:272192/248‑1 (MQ=255)

                                                                                ccGTAGGTTGTGCGAAAGCGGCACAGGACGACATCCTCAAAGCGTACGAAGCATACGATTTCCACGAAGTGGAACAGCGTCTGGTGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACCCCGCCAAAGCGCCCCGCGTGGCGCGTCGTAG‑CTCCCATACTGCGCTGCATACCATCGCCGCAGAGCTGGTGCGCTGGATGGCACCAATCCTCC‑CCttacccact                                                                                                                                                                  >  2:498618/1‑244 (MQ=255)

                                                                                  gTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCtg                                                                                                                                                                 >  1:625349/1‑250 (MQ=255)

                                                                                    aGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCtgatg                                                                                                                                                              >  1:305606/1‑251 (MQ=255)

                                                                                           gCGAAAGCGGCACAGGAAGCCATCCTAAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCCGAATGCGCTGTATAACATAGCAGAAGCGCTGGTTCGCCGGATGTCACCAAGCCTCC‑CCTTCACCCCTGGTGAAggggg                                                                                                                                                       >  2:149640/1‑247 (MQ=255)

                                                                                             gagaGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTAGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTACTTCTACCTCGACATCATCAAAGACCGTCAGTCCACCACCAAACCGGACAGCGTGGCGCGTCGTAA‑CTGCCCGACTGCGCTGTATCACATCGCAGTAGCGCTGGAGCGCTAGATGGCACCAAGCTACT‑CCTTCACCGCCGATGCAGGGTggg                                                                                                                                                     >  2:152385/4‑251 (MQ=255)

                                                                                              aaaGCGGCACAGGAAGACATCCTCCAGGCGTGCGAAGAATACGATTTCCACGAAGTGGTACAGCGTCTGATGAGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGAGCTGCATCACATCGCAGAAGCGCTGGTTCGCCGGATGGCCCCAATCCTCT‑CCTTAACCGCAGATGAGGTGTgggg                                                                                                                                                    >  2:670625/1‑251 (MQ=255)

                                                                                              aaaGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTgggg                                                                                                                                                    >  1:366305/1‑251 (MQ=255)

                                                                                                aGCGGCACAGGAAGACCTCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCt                                                                                                                                                  >  1:168713/1‑251 (MQ=255)

                                                                                                 gCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGAGTTCCACGAAGTGTTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCCCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTTCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTa                                                                                                                                                 <  1:620013/251‑1 (MQ=255)

                                                                                                    gCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCt                                                                                                                                              >  1:424326/1‑251 (MQ=255)

                                                                                                     cccaGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCAGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTg                                                                                                                                             <  1:345344/249‑1 (MQ=255)

                                                                                                      acaGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGCTGAAGTGTGGGGATACCTGc                                                                                                                                            >  1:615425/1‑251 (MQ=255)

                                                                                                        aGGAAGACCTCCACAAGGCGTACGAAGAATACGATTTCCACGAAGTGGTACAGCGTCTGATGCTCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGAACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCg                                                                                                                                          <  2:575909/251‑1 (MQ=255)

                                                                                                        aGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCg                                                                                                                                          >  1:586418/1‑251 (MQ=255)

                                                                                                                 tCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATTGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGt                                                                                                                                 >  1:600778/1‑251 (MQ=255)

                                                                                                                     cAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGCACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGac                                                                                                                              >  1:437745/1‑249 (MQ=255)

                                                                                                                      aaGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGaaa                                                                                                                             >  1:502209/1‑250 (MQ=255)

                                                                                                                        ggCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAAt                                                                                                                          >  1:660385/1‑251 (MQ=255)

                                                                                                                          cGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATAc                                                                                                                        <  1:6997/251‑1 (MQ=255)

                                                                                                                               gAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTCTCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAGGTGTGGGGCTCACAGCCGGGCGGACGTGAAAAATACGTCtt                                                                                                                   >  2:450383/1‑251 (MQ=255)

                                                                                                                                  gCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGTACGTGAAAAATACGTCTTCAc                                                                                                                >  2:446061/1‑251 (MQ=255)

                                                                                                                                       cGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTg                                                                                                           >  1:457543/1‑251 (MQ=255)

                                                                                                                                         attttcactaagtgttccaacGTCTGATGCGCTTTTGCGCCGTTGATATGGGTTCCTTCTACCTCGACATCACCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAg                                                                                                         <  2:509168/231‑1 (MQ=255)

                                                                                                                                                   aGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACAGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGACGCGCTGGATCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGCGTGGTACGa                                                                                                 >  1:657817/1‑249 (MQ=255)

                                                                                                                                                     tGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGc                                                                                             >  1:553044/1‑251 (MQ=255)

                                                                                                                                                           ggCGTCTGATGGGCTGTTGCCCTGTTGGGATGGGTTACTTCTACCGCGACATCCTCAAATACCGGCAGTACTCTGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGttt                                                                                       <  2:555585/250‑1 (MQ=255)

                                                                                                                                                           aGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGttt                                                                                       <  1:436000/251‑1 (MQ=255)

                                                                                                                                                              gTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTggt                                                                                    >  1:89361/1‑250 (MQ=255)

                                                                                                                                                              gTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTggt                                                                                    <  1:520612/251‑2 (MQ=255)

                                                                                                                                                                     gcgcTTCTGCCCCGTAGGTATGGGTTCCTCCTACCTCGACATCATCAAAGCCCGCCAGTCCACCTCCAAAGCGGACAGCGTGGCGCGGCGTAG‑CTGCCAGACTGCGCTGTAGCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGGCTGGCAg                                                                             <  2:660385/251‑1 (MQ=255)

                                                                                                                                                                      cgcTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGa                                                                            <  1:149640/251‑1 (MQ=255)

                                                                                                                                                                                ccGTTGAGATGGGTTCCTTCTCCCTCGACACCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGGGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                  <  2:305606/251‑2 (MQ=255)

                                                                                                                                                                                 cGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGca                                                                  >  1:485911/1‑249 (MQ=255)

                                                                                                                                                                                  gTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGCACGTCAGTACAACGCCAAAGCGGACAGCGTGCCGCGTTGTAG‑CTGCCAGACCGCGCTCTATCACATCGAAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTACTGAAGTGTGGGGCTACCTGCCGGGCGACCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGTCTGGCAGACAGTGAAGcatt                                                                >  1:286356/1‑248 (MQ=255)

                                                                                                                                                                                    tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑Tg                                                              >  2:618071/1‑250 (MQ=255)

                                                                                                                                                                                      agaTGGGTTCCTTCTACCTCGACATCATCAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCCCAGAAGCGCGGGGTCGCTGGAAGGCACCAATCCTCT‑CCTTCACCGCTGATGTAGTGTGGGGCTACCTGCCGGTCGAGCGTGAAAAATACGTCTTCACCGGGGAGTGGTCAGAAGGACTGTTTGTTCTGGCAGACAGTgcaacaatgaac                                                            >  2:539698/1‑240 (MQ=255)

                                                                                                                                                                                      agaTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAc                                                           <  1:651974/251‑1 (MQ=255)

                                                                                                                                                                                      aactGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGAGCGTCGTAG‑CTGCCAGACTGCGCTGGATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCCCCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTTGCAGACCGTGAAGCAA‑TGAAc                                                           <  2:444006/248‑1 (MQ=255)

                                                                                                                                                                                            gTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGGGTGGGGCTACCTGCCGGGCGCACGTGAAAAATACGTCTTCACCGGTGGGGGGTACGAAGGCCGGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAAcaatacg                                                     >  2:19025/1‑245 (MQ=255)

                                                                                                                                                                                             ttCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGTGGTACGCAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGt                                                    >  1:587058/1‑251 (MQ=255)

                                                                                                                                                                                                   cTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTggg                                              >  1:433730/1‑251 (MQ=255)

                                                                                                                                                                                                            aattaccaAAGACCGACAGTACACTGCCAAAGCGGACAGCGTGGATCGTCGTAG‑CTGCAAGACTGCGCTGTATCCCATCGCAGAATCGCTGGTTCGCTGGATGGCACCATTCCGCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTGGGGCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGt                                     <  2:386448/245‑1 (MQ=255)

                                                                                                                                                                                                                  caAAGCCCGTCAGAACCCCGCCAAAGCGGACAGCGTGGCGCTTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGaa                                 <  2:433730/249‑1 (MQ=255)

                                                                                                                                                                                                                  caAAGACCGGCAGAACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAG‑CTGCCAGAGTGCGCTGTATAACATCGCAGCAGCGCTGGCACGCTGGAGGGCACTAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTGCATGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAG‑GACAGAACGATGCGTTCGGGGACGATGTGTTGac                                 >  2:51373/1‑248 (MQ=255)

                                                                                                                                                                                                                  aaaaGACCGTCCGTACTCCGCCAAAGCGGACAGCGGGGCGCGGCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGATGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAg                               <  2:327459/250‑1 (MQ=255)

                                                                                                                                                                                                                    aaGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGCAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGACCGTGTAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGGAAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTg                             >  2:201536/1‑251 (MQ=255)

                                                                                                                                                                                                                     agtccGTCAGTACACCGCCAAGGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGc                            <  1:452336/248‑1 (MQ=255)

                                                                                                                                                                                                                        ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                         >  1:512676/1‑251 (MQ=255)

                                                                                                                                                                                                                         cGTCAGTACACCGCCAAAGCGGACAGAGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGCAGTGTGGGGCTACCTGCCGGGCGAACGTGAAACATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGACCGATGAGTTCTGGGCCGAGCTGTTGAAAGCGCGTgg                        >  2:90250/1‑251 (MQ=255)

                                                                                                                                                                                                                           gcagaccccccacAAAGCGGCCAGCGTGGCGCGTAGTAG‑CTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGGTTGATGGCACCAATCCGCC‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                        <  2:17430/237‑1 (MQ=255)

                                                                                                                                                                                                                            cAGTACACCGCCAAAGCGTACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGATCTTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                      <  1:440364/250‑1 (MQ=255)

                                                                                                                                                                                                                             aGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGa                     >  1:120741/1‑250 (MQ=255)

                                                                                                                                                                                                                                accccGCCAAAGCGCACAGCGTGGCGCGCCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCATAAGCGCTGGTTCGCTGGATGGCTCCAA‑CCCCTGCCTTCACCGCTGATGAAGTGTGGGGCCACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCGGGGACGAGCTGTTGAAAGGGCGTGGCGAAGt                  <  2:55189/247‑1 (MQ=255)

                                                                                                                                                                                                                                 cacCGCCAAAGCGGACAGGGGGGCGGGGCGTCG‑CTGCACGACTGCGCTGTATCCAATCGCAGAAGCGCTGGGTCGCGGGATGGCACCAATCTTCT‑CCTGCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGGTCTGGGACGAGCTGTGGAAAGTGCGTGGCGAAGTGa                <  2:110517/251‑1 (MQ=255)

                                                                                                                                                                                                                                  cccGCCAAAGCGGCCTGCGGGTCGCGTCGTAG‑CTGCCAGACTGCGCTTTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa               <  2:73324/250‑1 (MQ=255)

                                                                                                                                                                                                                                  cccGCCAAAGCGGACAGCGTGGCGCGTCGTAG‑CTGCCATACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGaa               <  1:389554/250‑1 (MQ=255)

                                                                                                                                                                                                                                         aaGCGGACAGCGTAGCGAGACGTAG‑CTGCCAGACTGAGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGAACAAATCCTCT‑CCTTCACCGCTGATGAAGAGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGTGGAGAAGTGACCACAGTc        >  2:449085/1‑251 (MQ=255)

                                                                                                                                                                                                                                              gACAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg    >  1:296392/1‑250 (MQ=255)

                                                                                                                                                                                                                                               aCAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGTCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGAGGCGAAGTGGACAAAGTCATTg    >  2:554332/1‑249 (MQ=255)

                                                                                                                                                                                                                                               aCAGCGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAg  <  1:432931/251‑1 (MQ=255)

CCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAG‑CTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCT‑CCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGA‑TGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAG  >  NC_000913/24373‑24862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`