BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,340	T→C	D650D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,340	0	T	C	97.1%	341.2 / NA	105	D650D (GAT→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (53/49); minor base A (2/1); total (55/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATC  >  NC_000913/24094‑24583

ttGGTTCATGTCTTCCCTAATGATCTCAACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACACACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACAAAACCGGAGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCt                                                                                                                                                                                                                                                 >  1:324313/2‑251 (MQ=255)

  ggTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAt                                                                                                                                                                                                                                               <  1:598877/251‑1 (MQ=255)

     tCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcg                                                                                                                                                                                                                                             <  1:490554/250‑1 (MQ=255)

      cATGTCTTCCCTAAGGATCCCCACCGCGATGAAAGGCAAAGCGCCGTAGCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcgt                                                                                                                                                                                                                                            <  2:158639/250‑1 (MQ=255)

                cTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTa                                                                                                                                                                                                                                  >  1:287163/1‑250 (MQ=255)

                 tAATGATCTCCAACGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACACCGTTTCGCCGCAGGTAGTGATGAACAAAATGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATAGTCGTATCCGTaa                                                                                                                                                                                                                                 >  2:397981/1‑250 (MQ=255)

                  aaTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTaa                                                                                                                                                                                                                                 <  1:432810/249‑1 (MQ=255)

                     gATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc                                                                                                                                                                                                                             <  1:430337/250‑1 (MQ=255)

                     gATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcg                                                                                                                                                                                                                            >  1:679956/1‑251 (MQ=255)

                      aTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTACCACcgc                                                                                                                                                                                                                           >  1:123298/1‑251 (MQ=255)

                      aTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcgc                                                                                                                                                                                                                           >  1:271303/1‑251 (MQ=255)

                            aCCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATG‑GTCCGGGCCGCAAGATGTCTAAATACATCGGCAACACCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGCGGCATACACCGCCGACACCGGTGAAAAAGACGTTTCTGACGAGATCCTGAAAAGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCtt                                                                                                                                                                                                                     >  2:544916/1‑251 (MQ=255)

                             ccGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTTGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTc                                                                                                                                                                                                                    <  1:338661/251‑1 (MQ=255)

                                cgATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctt                                                                                                                                                                                                                 >  2:283495/1‑250 (MQ=255)

                                  aggaaagtcaaaGCGCCGTATCGGCAGGTACTGAACCAAGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAAACCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACTCCGCGCGCTTCctgca                                                                                                                                                                                                               <  2:35340/242‑2 (MQ=255)

                                    gaaaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTgg                                                                                                                                                                                                             >  1:620685/8‑251 (MQ=255)

                                    gaaaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTgg                                                                                                                                                                                                             >  1:306129/8‑251 (MQ=255)

                                     aaaggcaaaGCGCTGTATCGTCAGGTACTGACCCACGGCGTTACCGGGGATG‑GTCAGGGCCGCAAGATGTTTAAATCCATCGGCAACCCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTATCACCGCGCGCTTCCTGCTGGc                                                                                                                                                                                                            <  2:664338/245‑1 (MQ=255)

                                         gcaaaGGGCCGGATCGTCAGGTACTGATCCACGGCTTTACCGTGGATG‑GCCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGTTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGGCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAc                                                                                                                                                                                                        <  2:452913/249‑1 (MQ=255)

                                          caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTTTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                                                                                                                                                       >  1:10230/2‑251 (MQ=255)

                                          caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                                                                                                                                                       <  1:203035/250‑1 (MQ=255)

                                          caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                                                                                                                                                       >  1:268152/2‑251 (MQ=255)

                                           aaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCt                                                                                                                                                                                                      <  2:268152/251‑1 (MQ=255)

                                             aGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTg                                                                                                                                                                                                     >  1:467860/1‑250 (MQ=255)

                                                gcCGTACCGTCAGGGACTGACCCCCGGCTTTACCGTGGATG‑GTCAGGGCCACAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACCGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACCCCGGTGAAATGGCCGTTTCTGACGCTATCCTGCAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTTCTGGCTAACCTTAACg                                                                                                                                                                                                 <  2:452131/251‑1 (MQ=255)

                                                    tATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGttt                                                                                                                                                                                             >  1:640269/1‑251 (MQ=255)

                                                     aTCGTCAGGTACTGCCCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGttt                                                                                                                                                                                             <  2:235620/250‑1 (MQ=255)

                                                     aTCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACCAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGCGAAATGGCCGTTTCTGCCGGGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCCCGCTTCCTGCTGGCAAACCTGAACGGtttt                                                                                                                                                                                            >  2:165054/1‑251 (MQ=255)

                                                     aTCGTCAGGTACTGACCCACGGCTTTACCGTGGAGG‑GTCAGGGCCGCAAGATTGCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACgggtt                                                                                                                                                                                             <  2:303450/250‑4 (MQ=255)

                                                     aTCGTAAGGTACTGACCCACGGCTTTACCTTGGATG‑TTCAGGGCCTAAAGATGTACAATTCCACCTGCAACACCGTTCAGCCGCAGGATGTTATGAACAAACGGGGCGCAGATATTCTT‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATTCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGtttt                                                                                                                                                                                            <  2:181869/251‑1 (MQ=255)

                                                        gTCAGGTACTCACCCCCGGCTTAACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCGAAACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTAGGTGGAATCAACCGACTACACCGGTGAACTGGCCGTTTCTGACGAGATCCTGAAAAGTGCTGCCGAAAGCTATCGTCGTATCAGTAACAACGCGCGATTCCTGCTGGCACACATGAGCGGTTTTGAt                                                                                                                                                                                         >  2:297878/1‑251 (MQ=255)

                                                          cAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATc                                                                                                                                                                                        <  1:373201/250‑1 (MQ=255)

                                                                 tGACCCACGGCTTTACCGTGGCTG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTTGCAAACCTGACCGGTTTTGATCCAGCaaa                                                                                                                                                                                 >  2:82821/1‑250 (MQ=255)

                                                                      cACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGAATCCGCAACACCGCGCGCATCCTCCTGGCAAACCTGAACGGTTTTGATCCAGAAAAAgtaat                                                                                                                                                                           >  2:487878/1‑247 (MQ=255)

                                                                       acgtcTTTACCGTGG‑TGTGTCAGGGCCGCAATATGTCAAAAGCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCTGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTTCACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCGTCCGACAGCTATCGTCTTATCCGTAACACAGCGCGCTTCCTGCTGGCAATCCTGAACGGTTTTGATCAAGCAAAAGATATg                                                                                                                                                                          <  2:671084/247‑1 (MQ=255)

                                                                         ggCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGaa                                                                                                                                                                                                   >  1:387166/1‑224 (MQ=255)

                                                                                        g‑gTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTGTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCGG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAt                                                                                                                                                         <  1:458646/251‑1 (MQ=255)

                                                                                                  cGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctg                                                                                                                                                                                                                 <  1:152959/186‑1 (MQ=255)

                                                                                                  cGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctg                                                                                                                                                                                                                 >  2:152959/1‑186 (MQ=255)

                                                                                                      aGATGTCTAAATCCATCGTCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGAt                                                                                                                                            >  1:272192/1‑251 (MQ=255)

                                                                                                       gATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATc                                                                                                                                           <  1:440629/251‑1 (MQ=255)

                                                                                                         tGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGc                                                                                                                                         >  1:575909/1‑251 (MQ=255)

                                                                                                          gtcaaaaTCCATCGGCAACACCTGTTCTCCGTAGGATGTGAGGAACAAACGGGGCGCGGATATTCTG‑CGTCTGGGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTGTCTGACGAGAGCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACTGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCt                                                                                                                                        <  2:659389/247‑1 (MQ=255)

                                                                                                          gTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGCCTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCCGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATctct                                                                                                                                        >  2:575982/1‑248 (MQ=255)

                                                                                                            cTAAATCCATCGGCAACACCGTTTCACCGCAGGATGAGATGCACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGTCATCAACCGACTACACCGGTGCAATGGCAGTTTCTGACGAGATCCTGAAACGTGCTCCCGGCCACTATCGTCGTAGCCGTCACACCGCACGCTTCCTGCTGGCACACATGAACGGTTTTGATCCCACCATCGAAATGGTGAAACCGGAAGAGATGGGGGTACTGGCACGCTgg                                                                                                                                      >  2:246611/1‑251 (MQ=255)

                                                                                                              aaaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTggg                                                                                                                                     <  1:18447/250‑1 (MQ=255)

                                                                                                              aaaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTgg                                                                                                                                      <  1:21700/249‑1 (MQ=255)

                                                                                                              aaaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTg                                                                                                                                       <  1:361637/248‑1 (MQ=255)

                                                                                                               aaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGcc                                                                                                                                   >  1:666869/1‑251 (MQ=255)

                                                                                                                  ccaacGGCAACACCTTTTCGCCGCCGGATGTTATGAACAAACTGTGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTTAAACGTGCTGCCGACAGCTAGCGTCGTAGCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAATAGATGGTGGTACTGGATCGCTGGGcctt                                                                                                                                 <  2:640269/246‑3 (MQ=255)

                                                                                                                  ccATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGt                                                                                                                                 >  1:239425/1‑250 (MQ=255)

                                                                                                                   cATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGt                                                                                                                                 >  1:119093/1‑249 (MQ=255)

                                                                                                                      cGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGtt                                                                                                                            >  1:191121/1‑251 (MQ=255)

                                                                                                                           acaCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCg                                                                                                                       <  1:201896/249‑1 (MQ=255)

                                                                                                                             aCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGaa                                                                                                                     <  1:584352/251‑1 (MQ=255)

                                                                                                                                gTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGCAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtc                                                                                                                                                                                                                                              >  1:629526/1‑127 (MQ=255)

                                                                                                                                gTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTTACGAGATCCTGAAACGTGCTGCCGACAGCTAtc                                                                                                                                                                                                                                              <  2:629526/127‑1 (MQ=255)

                                                                                                                                gTTTAGCCGCAAGTTGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGAATACACAGGAGAAATGGCAGTTTCTGACGCGATACTGAAACGCGCTGCCGAAAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGTCAAACATGAACGGTTTTGACCCAGGCACAGATATGGTGAAACCGGAAGGGATGGTGGAACTGGGACGCGGGGCCGtgcgtggtgtgaaagc                                                                                                                  >  2:432931/1‑236 (MQ=255)

                                                                                                                                        gcAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAgg                                                                                                          >  1:556565/1‑251 (MQ=255)

                                                                                                                                        gatggATGTTAGGAACAAACTGGGCGCGGATAG‑CTGGCGTCTGTGGGTGGCATCAACAGACTACGGCGGGGAAATGGCCGTTTCTGCCGAGATCCTGAAACGTGCTGCCTACAGCTATCGTCGTATCCGTAACACCGCGCGCTGCCTGCGGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAACCCGGAAGAGATGGTGGTACTGGAGCGCTGGTCCGTAGGTTGTGCGAAAGCGGTACAgg                                                                                                          <  2:306718/248‑1 (MQ=255)

                                                                                                                                         cAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCCAACCTGCACGGTTTTGATCCAGCACAAGATATGGTGAAACCGGAAGCGATGGTGGTACTGGATCGCTGGGCCGTGGGTTGTGCGAAAGCGGcaaatg                                                                                                          >  2:389909/1‑246 (MQ=255)

                                                                                                                                            gATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGa                                                                                                         >  1:21227/1‑248 (MQ=255)

                                                                                                                                             aTGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGAc                                                                                                     <  1:175161/251‑1 (MQ=255)

                                                                                                                                                 gatgaACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATcc                                                                                                 >  1:535029/1‑251 (MQ=255)

                                                                                                                                                    gaACAAACTGGGCGCGGATATTCTG‑CGTCGGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCa                                                                                              <  1:616111/251‑1 (MQ=255)

                                                                                                                                                     aaCAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCa                                                                                              >  1:386448/1‑250 (MQ=255)

                                                                                                                                                       cAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAgg                                                                                           >  1:624984/1‑251 (MQ=255)

                                                                                                                                                             gggCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTa                                                                                       <  2:620685/249‑1 (MQ=255)

                                                                                                                                                             gggCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACg                                                                                     <  1:389909/251‑1 (MQ=255)

                                                                                                                                                                 ggcgaatattcgggttCTGTGGGTAGCATCAACCGACTACACCGGTGAAATGGCCGTTGCTGACTAGATCCTGAAACGTGCTGCCGACAGCTAGCGTCGTATCCGTAACACCGCGCGCTTCCTGATGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAATACAGCCTAAAGGCGTACGAAg                                                                                  <  2:96624/236‑1 (MQ=255)

                                                                                                                                                                     atatTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATAc                                                                             >  1:227343/1‑251 (MQ=255)

                                                                                                                                                                       atTCTG‑CGTCTGTGGGTGGCATCACCCGACTACACCGGTGAAATTGCAGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACAGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCTAAAGCTATGGTGAAACCGGGAGCGCTGGTGGTACTGTATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACCGGAAGACATCCGCAAGGCGTACGGAGCAtgcgg                                                                           >  2:332016/1‑247 (MQ=255)

                                                                                                                                                                         gcTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAGCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGAt                                                                          <  1:652344/249‑1 (MQ=255)

                                                                                                                                                                                 cTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACg                                                                   >  1:156274/1‑250 (MQ=255)

                                                                                                                                                                                  tgtgGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCgg                                                                                                                <  1:145556/204‑1 (MQ=255)

                                                                                                                                                                                   gggggtggCATCAACAGATTACACCGGTGAAAGGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGaa                                                                 <  2:21227/248‑1 (MQ=255)

                                                                                                                                                                                    tgggtggCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTCGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATCCGCTTTCCACGAAgg                                                               >  2:89514/1‑250 (MQ=255)

                                                                                                                                                                                        tggCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGt                                                            >  1:555585/1‑250 (MQ=255)

                                                                                                                                                                                           cATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAg                                                        <  1:675758/251‑1 (MQ=255)

                                                                                                                                                                                           cATCAACCGACGACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGGGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCGGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAg                                                        <  1:297878/251‑1 (MQ=255)

                                                                                                                                                                                            aTCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCACGGCGTACGAAGCATGAGATTTCCACGAAGTGGGACAGc                                                       >  2:652321/1‑251 (MQ=255)

                                                                                                                                                                                                   gACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGAt                                                >  1:668367/1‑251 (MQ=255)

                                                                                                                                                                                                    ccTACACCGGTGAAATGGCCGTTTTTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATg                                               <  2:10230/250‑1 (MQ=255)

                                                                                                                                                                                                         cccGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCtt                                          <  1:9941/250‑1 (MQ=255)

                                                                                                                                                                                                             gTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGc                                      >  1:572005/1‑251 (MQ=255)

                                                                                                                                                                                                             gTGAAATGGCCGTTTCTGACGAGAGCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGc                                      <  2:666869/251‑1 (MQ=255)

                                                                                                                                                                                                                aaaTGGCCTTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTcc                                   <  1:89514/251‑1 (MQ=255)

                                                                                                                                                                                                                 aaTGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCg                                  >  1:40921/1‑251 (MQ=255)

                                                                                                                                                                                                                    ggCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGCTCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGACGAAGGTTGTGCGAAAGCGGCACAGGGAGACATCCTCAAGGCGTACGCAGACTACGATTTCCACGAAGTGGTACCACGTCTGATGCGCTTCTGCTCCGTTg                               >  2:651974/1‑251 (MQ=255)

                                                                                                                                                                                                                    ggCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACa                                                                                                    <  1:247057/182‑1 (MQ=255)

                                                                                                                                                                                                                    ggCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGtt                                <  1:397981/250‑1 (MQ=255)

                                                                                                                                                                                                                       cGTTTCTGACGAGATCCTGTAACGTGCTGCCGTAAACTATCGTCGTTTCCGTAACACCGCGCGCTGCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTgaga                            <  2:624984/251‑1 (MQ=255)

                                                                                                                                                                                                                               acgtgatacTGAAACGGTCTGCCGACAGCTATCTTCGTATCCGTAATACCGTGCGTTTCCTGCTGGCAAACCTTAACGGTTTTGATCCAGCAAAAGATATGGGGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTGGTGCGAAAGCGGCACAGGAATACATCCGCAAGGCGTACGAAGCATACGATTACCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCGCCGTTGAGATGGGTTcc                    <  2:72595/243‑1 (MQ=255)

                                                                                                                                                                                                                                cGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGAGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCt                   >  1:442378/1‑251 (MQ=255)

                                                                                                                                                                                                                                 gagaTCCTGAAACGGGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCGGGCAAAACTGCACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATAGTGGTACTGGATAGCTGCGCCGTAGGTTGAGCGAAAGCGTGACAGGAAGACATCCTCAGTGCGTAAGACGCATACGAGTTCCACGAATCGGTACAGAGTCTGCTGCACTTCTGCTCCGATGAGCTGGGTGCCtt                  >  2:606424/1‑251 (MQ=255)

                                                                                                                                                                                                                                 gagaTCCGGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCtt                  <  2:535029/251‑1 (MQ=255)

                                                                                                                                                                                                                                  agaTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTc                 >  1:303370/1‑251 (MQ=255)

                                                                                                                                                                                                                                            aCGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAca       <  1:283495/251‑1 (MQ=255)

                                                                                                                                                                                                                                              gTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGGGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCATCAAGGCGTACGAAGCATACGATATGCACGCAGTGGTACAGCGGCTGATGCGCTTCTGCTCCGTTGAGAGGGGTTCCTTCCACCACTAcatc     >  2:16036/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 ctgcCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACatcatc  >  2:497875/1‑251 (MQ=255)

CTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTG‑CGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATC  >  NC_000913/24094‑24583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`