Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,217	T→C	N609N (AAT→AAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,217	0	T	C	96.7%	269.2 / ‑3.0	95	N609N (AAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (42/46);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

GCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTT  >  NC_000913/23971‑24461                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gccgtatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGGTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCg                                                                                                                                                                                                                                                  <  1:496008/235‑1 (MQ=255)   cggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGtt                                                                                                                                                                                                                                                <  1:195839/237‑1 (MQ=255)        actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTcgcc                                                                                                                                                                                                                                           <  1:160846/242‑1 (MQ=255)                   aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                                                                                                                                                 >  1:592433/1‑250 (MQ=255)                    tGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAACGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTAGCCGCAGGAttggat                                                                                                                                                                                                                               >  2:373201/1‑246 (MQ=255)                      gTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGCGTCAGGCATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATATCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGTCTTTACCGTGGATG‑GGCAGAGCCCGAAGTTGTCTAAATCCATCGGCAAGCCCTGTTCGACGCAtgatgtgatg                                                                                                                                                                                                                              >  2:345344/1‑250 (MQ=255)                          gaccccGGATCTGCCCACTCTTCTGTTGGTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaa                                                                                                                                                                                                                         <  2:369569/247‑1 (MQ=255)                          gACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGTTTCGCCGCAGGATGTGATGAACa                                                                                                                                                                                                                          >  1:500826/1‑250 (MQ=255)                                 gATCTACCCACTCTTCTGTTGTTGCCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTACATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACCAAATGGgc                                                                                                                                                                                                                  >  2:675758/1‑251 (MQ=255)                                  aTCTACACACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCCACGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTAAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCCAGATGTCTAAATCCATCGGCAAACCCGTTTCGCCGCAGGATGTGATGACCAAACTGGgcg                                                                                                                                                                                                                 >  2:430337/1‑251 (MQ=255)                                   tCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGATGAACAACCTGGgcgc                                                                                                                                                                                                                >  2:440629/1‑251 (MQ=255)                                   tCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAAGGCTTTCCCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGATGAACAAACTGGgcgc                                                                                                                                                                                                                >  2:361637/1‑251 (MQ=255)                                     tACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCgg                                                                                                                                                                                                              >  1:158639/1‑251 (MQ=255)                                      aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGGTGAACAAACTGGGCGCgg                                                                                                                                                                                                              >  2:490554/1‑250 (MQ=255)                                      aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGgc                                                                                                                                                                                                                  <  1:30757/246‑1 (MQ=255)                                             cttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTc                                                                                                                                                                                                        <  2:677099/249‑1 (MQ=255)                                              ttctGTTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAACAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGAGCgggaattcttg                                                                                                                                                                                                     >  2:21700/1‑242 (MQ=255)                                              ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTg                                                                                                                                                                                                      <  2:498938/250‑1 (MQ=255)                                               tctGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCTTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCAGCGTCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCt                                                                                                                                                                                                       <  1:77068/248‑1 (MQ=255)                                                  gttgttGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTc                                                                                                                                                                                                  <  1:98114/250‑1 (MQ=255)                                                  gttgttGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCt                                                                                                                                                                                                 <  1:374737/251‑1 (MQ=255)                                                     gttgACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg                                                                                                                                                                                              <  1:194626/251‑1 (MQ=255)                                                        gACGTGCGTCCGGAATTTGCCGGTCAAGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCACTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTAAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCACGATGTCTAAATCCATCGGCACCACCGTTTCTCCGCAGGCTGGGATGAACAAACTGGGCGAGGATATTCTGCGTCTgtgggg                                                                                                                                                                                           >  2:616111/1‑250 (MQ=255)                                                        gACGTACGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCTACGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGGTG‑GTCAGGGCCGCAAGATGTCTAAATCCCTAGGCAATACCGTTTAGCCGGAGGATGTGCTTAACAAACTGGGCGCGGTAATTCTGGGCCTgtgggg                                                                                                                                                                                           >  2:367873/1‑250 (MQ=255)                                                          agggtgttcGGAATTTGCCTGTCTGGCCGCGGACATGTATATGGAAGGTACGGACCAGCCACGTGGGTGGTTCATGTCGTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTAGCGTCAGGTACTGACCCACGGTTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgttggtgg                                                                                                                                                                                         <  2:579914/243‑1 (MQ=255)                                                                              gTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGt                                                                                                                                                                     <  1:626121/251‑1 (MQ=255)                                                                                    caggtgACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCTTGTCGTCCTTAATGATCTCCCCCGCGATGAAAGGCCAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGGGGATG‑GTCAGGGTCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTTGGCGCGGATATTCTGCGTCTGTGGTTGGCATCAGCCGCCTACACCGGTGAAATg                                                                                                                                                               <  2:262522/246‑1 (MQ=255)                                                                                      gCGGAAATGGATCTGGAAGGTGCTGACCAGCACCGTGGTTGTTTCATTTCTGCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACACCGTTGCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGc                                                                                                                                                             <  2:320650/251‑1 (MQ=255)                                                                                       gggACATGTATCTGGAAGGTTCTGACCTGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACTCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                                                                                                                                                            <  2:453286/250‑1 (MQ=255)                                                                                       cGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                                                                                                                                                            <  1:590565/251‑1 (MQ=255)                                                                                        ggACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCg                                                                                                                                                           <  1:343959/251‑1 (MQ=255)                                                                                         gACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGt                                                                                                                                                          >  1:504493/1‑251 (MQ=255)                                                                                             tGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTACTGACCCACGGCTTTACCGGGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACAACCACGGGGAAATAGCCGTTTCt                                                                                                                                                      >  2:622574/1‑251 (MQ=255)                                                                                                 tctgcaacgTTCTGACCAGCACCGGGGTTTGTTCATTTCTTCCCTAATGACCTCCACCGCGATGGAAGGCAAAGCGCCGTGTCGTCAGGTACTGACCCACGGCTTTAGCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCACCGGCAATACCTTTCCGCCTCAGGATGGGATCAACAAGCGGGGGTCGGAGAGGCTACGTCTGTGGGGGGGATCAATGGACTCCACCGGGGAAAtcgtcgggtctgac                                                                                                                                                   <  2:273574/242‑14 (MQ=255)                                                                                                         gTTCTGGCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTg                                                                                                                                          <  1:370772/251‑1 (MQ=255)                                                                                                               aCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt                                                                                                                                    <  1:296877/251‑1 (MQ=255)                                                                                                               aCCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt                                                                                                                                    <  1:367873/251‑1 (MQ=255)                                                                                                                  agcacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgct                                                                                                                                 >  1:143256/3‑251 (MQ=255)                                                                                                                   gcacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCACCACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCTGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgctg                                                                                                                                >  2:298486/2‑251 (MQ=255)                                                                                                                     accgtggtgggTTCATGTCTACCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTCTCGTCAGGTGCTTACCCACGGCTTTACCGTGGATT‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAATCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACTCCGGTGAAATGGCCGTTTCTGACGATATCCTGAAACGTGCTGcc                                                                                                                              <  2:453248/242‑1 (MQ=255)                                                                                                                        gtggttgtttCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGac                                                                                                                           <  2:592433/243‑2 (MQ=255)                                                                                                                            ttGGTTCATGTCTTCCCTAATGATCTCAACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACACACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACAAAACCGGAGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCt                                                                                                                       >  1:324313/2‑251 (MQ=255)                                                                                                                             tGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTCATGACCCACGGCTCTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGTCAACACCGTTTCGCCGCAGGATGGGATGGACAATCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATAAACAGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTCAAACGtgctgcacacagctc                                                                                                                      >  2:6997/1‑242 (MQ=255)                                                                                                                              ggTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAt                                                                                                                     <  1:598877/251‑1 (MQ=255)                                                                                                                                 tCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcg                                                                                                                   <  1:490554/250‑1 (MQ=255)                                                                                                                                  cATGTCTTCCCTAAGGATCCCCACCGCGATGAAAGGCAAAGCGCCGTAGCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcgt                                                                                                                  <  2:158639/250‑1 (MQ=255)                                                                                                                                            cTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTa                                                                                                        >  1:287163/1‑250 (MQ=255)                                                                                                                                             tAATGATCTCCAACGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACACCGTTTCGCCGCAGGTAGTGATGAACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATAGTCGTATCCGTaa                                                                                                       >  2:397981/1‑250 (MQ=255)                                                                                                                                              aaTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTaa                                                                                                       <  1:432810/249‑1 (MQ=255)                                                                                                                                                 gATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc                                                                                                   <  1:430337/250‑1 (MQ=255)                                                                                                                                                 gATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcg                                                                                                  >  1:679956/1‑251 (MQ=255)                                                                                                                                                  aTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTACCACcgc                                                                                                 >  1:123298/1‑251 (MQ=255)                                                                                                                                                  aTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcgc                                                                                                 >  1:271303/1‑251 (MQ=255)                                                                                                                                                        aCCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATG‑GTCCGGGCCGCAAGATGTCTAAATACATCGGCAACACCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGCGGCATACACCGCCGACACCGGTGAAAAAGACGTTTCTGACGAGATCCTGAAAAGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCtt                                                                                           >  2:544916/1‑251 (MQ=255)                                                                                                                                                         ccGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTTGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTc                                                                                          <  1:338661/251‑1 (MQ=255)                                                                                                                                                            cgATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctt                                                                                       >  2:283495/1‑250 (MQ=255)                                                                                                                                                              aggaaagtcaaaGCGCCGTATCGGCAGGTACTGAACCAAGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAAACCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACTCCGCGCGCTTCctgca                                                                                     <  2:35340/242‑2 (MQ=255)                                                                                                                                                                gaaaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTgg                                                                                   >  1:306129/8‑251 (MQ=255)                                                                                                                                                                gaaaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTgg                                                                                   >  1:620685/8‑251 (MQ=255)                                                                                                                                                                 aaaggcaaaGCGCTGTATCGTCAGGTACTGACCCACGGCGTTACCGGGGATG‑GTCAGGGCCGCAAGATGTTTAAATCCATCGGCAACCCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTATCACCGCGCGCTTCCTGCTGGc                                                                                  <  2:664338/245‑1 (MQ=255)                                                                                                                                                                     gcaaaGGGCCGGATCGTCAGGTACTGATCCACGGCTTTACCGTGGATG‑GCCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGTTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGGCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAc                                                                              <  2:452913/249‑1 (MQ=255)                                                                                                                                                                      caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTTTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                             >  1:10230/2‑251 (MQ=255)                                                                                                                                                                      caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                             >  1:268152/2‑251 (MQ=255)                                                                                                                                                                      caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                             <  1:203035/250‑1 (MQ=255)                                                                                                                                                                       aaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCt                                                                            <  2:268152/251‑1 (MQ=255)                                                                                                                                                                         aGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTg                                                                           >  1:467860/1‑250 (MQ=255)                                                                                                                                                                            gcCGTACCGTCAGGGACTGACCCCCGGCTTTACCGTGGATG‑GTCAGGGCCACAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACCGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACCCCGGTGAAATGGCCGTTTCTGACGCTATCCTGCAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTTCTGGCTAACCTTAACg                                                                       <  2:452131/251‑1 (MQ=255)                                                                                                                                                                                tATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGttt                                                                   >  1:640269/1‑251 (MQ=255)                                                                                                                                                                                 aTCGTCAGGTACTGCCCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGttt                                                                   <  2:235620/250‑1 (MQ=255)                                                                                                                                                                                 aTCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACCAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGCGAAATGGCCGTTTCTGCCGGGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCCCGCTTCCTGCTGGCAAACCTGAACGGtttt                                                                  >  2:165054/1‑251 (MQ=255)                                                                                                                                                                                 aTCGTCAGGTACTGACCCACGGCTTTACCGTGGAGG‑GTCAGGGCCGCAAGATTGCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACgggtt                                                                   <  2:303450/250‑4 (MQ=255)                                                                                                                                                                                 aTCGTAAGGTACTGACCCACGGCTTTACCTTGGATG‑TTCAGGGCCTAAAGATGTACAATTCCACCTGCAACACCGTTCAGCCGCAGGATGTTATGAACAAACGGGGCGCAGATATTCTTCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATTCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGtttt                                                                  <  2:181869/251‑1 (MQ=255)                                                                                                                                                                                    gTCAGGTACTCACCCCCGGCTTAACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCGAAACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTAGGTGGAATCAACCGACTACACCGGTGAACTGGCCGTTTCTGACGAGATCCTGAAAAGTGCTGCCGAAAGCTATCGTCGTATCAGTAACAACGCGCGATTCCTGCTGGCACACATGAGCGGTTTTGAt                                                               >  2:297878/1‑251 (MQ=255)                                                                                                                                                                                      cAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATc                                                              <  1:373201/250‑1 (MQ=255)                                                                                                                                                                                             tGACCCACGGCTTTACCGTGGCTG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTTGCAAACCTGACCGGTTTTGATCCAGCaaa                                                       >  2:82821/1‑250 (MQ=255)                                                                                                                                                                                                  cACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGAATCCGCAACACCGCGCGCATCCTCCTGGCAAACCTGAACGGTTTTGATCCAGAAAAAgtaat                                                 >  2:487878/1‑247 (MQ=255)                                                                                                                                                                                                   acgtcTTTACCGTGG‑TGTGTCAGGGCCGCAATATGTCAAAAGCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCTGATATTCTGCGTCTGTGGGTGGCATCAACCGACTTCACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCGTCCGACAGCTATCGTCTTATCCGTAACACAGCGCGCTTCCTGCTGGCAATCCTGAACGGTTTTGATCAAGCAAAAGATATg                                                <  2:671084/247‑1 (MQ=255)                                                                                                                                                                                                     ggCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGaa                                                                         >  1:387166/1‑224 (MQ=255)                                                                                                                                                                                                                    g‑gTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTGTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCGGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAt                               <  1:458646/251‑1 (MQ=255)                                                                                                                                                                                                                              cGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctg                                                                                       >  2:152959/1‑186 (MQ=255)                                                                                                                                                                                                                              cGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctg                                                                                       <  1:152959/186‑1 (MQ=255)                                                                                                                                                                                                                                  aGATGTCTAAATCCATCGTCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGAt                  >  1:272192/1‑251 (MQ=255)                                                                                                                                                                                                                                   gATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATc                 <  1:440629/251‑1 (MQ=255)                                                                                                                                                                                                                                     tGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGc               >  1:575909/1‑251 (MQ=255)                                                                                                                                                                                                                                      gtcaaaaTCCATCGGCAACACCTGTTCTCCGTAGGATGTGAGGAACAAACGGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTGTCTGACGAGAGCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACTGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCt              <  2:659389/247‑1 (MQ=255)                                                                                                                                                                                                                                      gTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGCCTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCCGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATctct              >  2:575982/1‑248 (MQ=255)                                                                                                                                                                                                                                        cTAAATCCATCGGCAACACCGTTTCACCGCAGGATGAGATGCACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGTCATCAACCGACTACACCGGTGCAATGGCAGTTTCTGACGAGATCCTGAAACGTGCTCCCGGCCACTATCGTCGTAGCCGTCACACCGCACGCTTCCTGCTGGCACACATGAACGGTTTTGATCCCACCATCGAAATGGTGAAACCGGAAGAGATGGGGGTACTGGCACGCTgg            >  2:246611/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTggg           <  1:18447/250‑1 (MQ=255)                                                                                                                                                                                                                                          aaaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTgg            <  1:21700/249‑1 (MQ=255)                                                                                                                                                                                                                                          aaaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTg             <  1:361637/248‑1 (MQ=255)                                                                                                                                                                                                                                           aaTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGcc         >  1:666869/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccaacGGCAACACCTTTTCGCCGCCGGATGTTATGAACAAACTGTGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTTAAACGTGCTGCCGACAGCTAGCGTCGTAGCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAATAGATGGTGGTACTGGATCGCTGGGcctt       <  2:640269/246‑3 (MQ=255)                                                                                                                                                                                                                                              ccATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGt       >  1:239425/1‑250 (MQ=255)                                                                                                                                                                                                                                               cATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGt       >  1:119093/1‑249 (MQ=255)                                                                                                                                                                                                                                                  cGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGtt  >  1:191121/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATG‑GTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTT  >  NC_000913/23971‑24461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑