Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,133	G→A	K581K (AAG→AAA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,133	0	G	A	97.5%	236.4 / ‑4.0	79	K581K (AAG→AAA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (33/44);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTG  >  NC_000913/23891‑24376                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgc                                                                                                                                                                                                                                             <  1:456145/242‑1 (MQ=255)   agttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgcgc                                                                                                                                                                                                                                           <  1:237181/244‑1 (MQ=255)    gttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTACCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                                                                                                                                                                                          <  1:616359/245‑1 (MQ=255)    gttaacggCATCCAGGAGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                                                                                                                                                                                          <  1:396483/245‑1 (MQ=255)         cgtcagccAGGCGGGGTGGGAGTACGATGCGAAAGAGATCATCGGCGACGAAGCGGACCAGGTTGAGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAACTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTGTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCTGTAta                                                                                                                                                                                                                                     <  2:224144/245‑2 (MQ=255)             aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTcc                                                                                                                                                                                                                                 >  2:286809/1‑250 (MQ=255)                  ggCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTAc                                                                                                                                                                                                                            <  1:364926/251‑1 (MQ=255)                   gCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACt                                                                                                                                                                                                                           <  1:669755/251‑1 (MQ=255)                                 cGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCtgt                                                                                                                                                                                                              <  2:153842/250‑3 (MQ=255)                                  gATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAc                                                                                                                                                                                                            <  1:156568/251‑1 (MQ=255)                                   aTGCGAAAGAGATCCTCGACGACGAAGCTGACCAGTATGTGAAAGTGACGGATACTCTC‑GACGTATGGTTTGACTCCGGATTTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGCGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCCACGTATCGTCAGGTACTGAccccaggcattaac                                                                                                                                                                                                           >  2:432810/1‑240 (MQ=255)                                     gCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCgg                                                                                                                                                                                                         >  2:203035/1‑250 (MQ=255)                                            agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGACCCACGGCTTTACCGTGGATggg                                                                                                                                                                                                  >  2:343959/1‑250 (MQ=255)                                                        acgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAg                                                                                                                                                                                      <  1:185067/251‑1 (MQ=255)                                                         cgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                                                                                                                                                                                                    <  1:325679/220‑1 (MQ=255)                                                             gCTGACCAGTATGTGAAAGTGCCGGATTC‑TCGCGATGTATGTTTTGACTCCGGAGCTAACCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc                                                                                                                                                                                 <  2:307374/251‑1 (MQ=255)                                                             gCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc                                                                                                                                                                                 <  1:286809/251‑1 (MQ=255)                                                                    tgcatgTGAAAGTGCCGGATACTCTA‑GACGTATGGATTGCCTCCGGATCTACCCACTCTTCCGTTGTTGACATGCGTCCGGAATTTGAAGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCCCCGTGGTTGGTTCATGTCTTACCTAATGATCTCCACCGCGAAGAAAGGCAAAGCGACGTATCGTCAGGTACTGCCACACGGCTTTCACGTGGATAGTCAGGGcagacggacggataatacc                                                                                                                                                                          >  2:620013/6‑233 (MQ=255)                                                                       atgTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAt                                                                                                                                                                        <  1:252532/248‑1 (MQ=255)                                                                              aagtgcccgatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATGGACAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                                                                 >  2:201896/21‑250 (MQ=255)                                                                                  gccgtatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGGTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCg                                                                                                                                                             <  1:496008/235‑1 (MQ=255)                                                                                    cggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGtt                                                                                                                                                           <  1:195839/237‑1 (MQ=255)                                                                                         actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTcgcc                                                                                                                                                      <  1:160846/242‑1 (MQ=255)                                                                                          ctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaa                                                                                                                                                                  >  1:614310/9‑238 (MQ=255)                                                                                          ctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaa                                                                                                                                                                  <  2:614310/230‑1 (MQ=255)                                                                                                    aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                                                            >  1:592433/1‑250 (MQ=255)                                                                                                     tGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAACGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTAGCCGCAGGAttggat                                                                                                                                          >  2:373201/1‑246 (MQ=255)                                                                                                       gTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGCGTCAGGCATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATATCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGTCTTTACCGTGGATGGGCAGAGCCCGAAGTTGTCTAAATCCATCGGCAAGCCCTGTTCGACGCAtgatgtgatg                                                                                                                                         >  2:345344/1‑250 (MQ=255)                                                                                                           gaccccGGATCTGCCCACTCTTCTGTTGGTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaa                                                                                                                                    <  2:369569/247‑1 (MQ=255)                                                                                                           gACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGTTTCGCCGCAGGATGTGATGAACa                                                                                                                                     >  1:500826/1‑250 (MQ=255)                                                                                                                  gATCTACCCACTCTTCTGTTGTTGCCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTACATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACCAAATGGgc                                                                                                                             >  2:675758/1‑251 (MQ=255)                                                                                                                   aTCTACACACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCCACGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTAAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCCAGATGTCTAAATCCATCGGCAAACCCGTTTCGCCGCAGGATGTGATGACCAAACTGGgcg                                                                                                                            >  2:430337/1‑251 (MQ=255)                                                                                                                    tCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGATGAACAACCTGGgcgc                                                                                                                           >  2:440629/1‑251 (MQ=255)                                                                                                                    tCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAAGGCTTTCCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGATGAACAAACTGGgcgc                                                                                                                           >  2:361637/1‑251 (MQ=255)                                                                                                                      tACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCgg                                                                                                                         >  1:158639/1‑251 (MQ=255)                                                                                                                       aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGGTGAACAAACTGGGCGCgg                                                                                                                         >  2:490554/1‑250 (MQ=255)                                                                                                                       aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGgc                                                                                                                             <  1:30757/246‑1 (MQ=255)                                                                                                                              cttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTc                                                                                                                   <  2:677099/249‑1 (MQ=255)                                                                                                                               ttctGTTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAACAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGAGCgggaattcttg                                                                                                                >  2:21700/1‑242 (MQ=255)                                                                                                                               ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTg                                                                                                                 <  2:498938/250‑1 (MQ=255)                                                                                                                                tctGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCTTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGTCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCt                                                                                                                  <  1:77068/248‑1 (MQ=255)                                                                                                                                   gttgttGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTc                                                                                                             <  1:98114/250‑1 (MQ=255)                                                                                                                                   gttgttGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCt                                                                                                            <  1:374737/251‑1 (MQ=255)                                                                                                                                      gttgACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg                                                                                                         <  1:194626/251‑1 (MQ=255)                                                                                                                                         gACGTGCGTCCGGAATTTGCCGGTCAAGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCACTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTAAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCACGATGTCTAAATCCATCGGCACCACCGTTTCTCCGCAGGCTGGGATGAACAAACTGGGCGAGGATATTCTGCGTCTgtgggg                                                                                                      >  2:616111/1‑250 (MQ=255)                                                                                                                                         gACGTACGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCTACGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGGTGGTCAGGGCCGCAAGATGTCTAAATCCCTAGGCAATACCGTTTAGCCGGAGGATGTGCTTAACAAACTGGGCGCGGTAATTCTGGGCCTgtgggg                                                                                                      >  2:367873/1‑250 (MQ=255)                                                                                                                                           agggtgttcGGAATTTGCCTGTCTGGCCGCGGACATGTATATGGAAGGTACGGACCAGCCACGTGGGTGGTTCATGTCGTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTAGCGTCAGGTACTGACCCACGGTTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgttggtgg                                                                                                    <  2:579914/243‑1 (MQ=255)                                                                                                                                                              ggTCACGCAGCTGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCa                                                                                                                                                                                                                     >  1:656861/1‑119 (MQ=255)                                                                                                                                                               gTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGt                                                                                <  1:626121/251‑1 (MQ=255)                                                                                                                                                                     caggtgACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCTTGTCGTCCTTAATGATCTCCCCCGCGATGAAAGGCCAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGGGGATGGTCAGGGTCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTTGGCGCGGATATTCTGCGTCTGTGGTTGGCATCAGCCGCCTACACCGGTGAAATg                                                                          <  2:262522/246‑1 (MQ=255)                                                                                                                                                                       gCGGAAATGGATCTGGAAGGTGCTGACCAGCACCGTGGTTGTTTCATTTCTGCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACACCGTTGCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGc                                                                        <  2:320650/251‑1 (MQ=255)                                                                                                                                                                        gggACATGTATCTGGAAGGTTCTGACCTGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACTCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                                                                       <  2:453286/250‑1 (MQ=255)                                                                                                                                                                        cGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                                                                       <  1:590565/251‑1 (MQ=255)                                                                                                                                                                         ggACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCg                                                                      <  1:343959/251‑1 (MQ=255)                                                                                                                                                                          gACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGt                                                                     >  1:504493/1‑251 (MQ=255)                                                                                                                                                                              tGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTACTGACCCACGGCTTTACCGGGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACAACCACGGGGAAATAGCCGTTTCt                                                                 >  2:622574/1‑251 (MQ=255)                                                                                                                                                                                  tctgcaacgTTCTGACCAGCACCGGGGTTTGTTCATTTCTTCCCTAATGACCTCCACCGCGATGGAAGGCAAAGCGCCGTGTCGTCAGGTACTGACCCACGGCTTTAGCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCACCGGCAATACCTTTCCGCCTCAGGATGGGATCAACAAGCGGGGGTCGGAGAGGCTACGTCTGTGGGGGGGATCAATGGACTCCACCGGGGAAAtcgtcgggtctgac                                                              <  2:273574/242‑14 (MQ=255)                                                                                                                                                                                          gTTCTGGCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTg                                                     <  1:370772/251‑1 (MQ=255)                                                                                                                                                                                                aCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt                                               <  1:296877/251‑1 (MQ=255)                                                                                                                                                                                                aCCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt                                               <  1:367873/251‑1 (MQ=255)                                                                                                                                                                                                   agcacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgct                                            >  1:143256/3‑251 (MQ=255)                                                                                                                                                                                                    gcacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCACCACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCTGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgctg                                           >  2:298486/2‑251 (MQ=255)                                                                                                                                                                                                      accgtggtgggTTCATGTCTACCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTCTCGTCAGGTGCTTACCCACGGCTTTACCGTGGATTGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAATCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACTCCGGTGAAATGGCCGTTTCTGACGATATCCTGAAACGTGCTGcc                                         <  2:453248/242‑1 (MQ=255)                                                                                                                                                                                                         gtggttgtttCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGac                                      <  2:592433/243‑2 (MQ=255)                                                                                                                                                                                                             ttGGTTCATGTCTTCCCTAATGATCTCAACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACACACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACAAAACCGGAGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCt                                  >  1:324313/2‑251 (MQ=255)                                                                                                                                                                                                              tGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTCATGACCCACGGCTCTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGTCAACACCGTTTCGCCGCAGGATGGGATGGACAATCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATAAACAGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTCAAACGtgctgcacacagctc                                 >  2:6997/1‑242 (MQ=255)                                                                                                                                                                                                               ggTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAt                                <  1:598877/251‑1 (MQ=255)                                                                                                                                                                                                                  tCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcg                              <  1:490554/250‑1 (MQ=255)                                                                                                                                                                                                                   cATGTCTTCCCTAAGGATCCCCACCGCGATGAAAGGCAAAGCGCCGTAGCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcgt                             <  2:158639/250‑1 (MQ=255)                                                                                                                                                                                                                             cTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTa                   >  1:287163/1‑250 (MQ=255)                                                                                                                                                                                                                              tAATGATCTCCAACGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACACCGTTTCGCCGCAGGTAGTGATGAACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATAGTCGTATCCGTaa                  >  2:397981/1‑250 (MQ=255)                                                                                                                                                                                                                               aaTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTaa                  <  1:432810/249‑1 (MQ=255)                                                                                                                                                                                                                                  gATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc              <  1:430337/250‑1 (MQ=255)                                                                                                                                                                                                                                  gATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcg             >  1:679956/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTACCACcgc            >  1:123298/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcgc            >  1:271303/1‑251 (MQ=255)                                                                                                                                                                                                                                         aCCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATGGTCCGGGCCGCAAGATGTCTAAATACATCGGCAACACCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGCGGCATACACCGCCGACACCGGTGAAAAAGACGTTTCTGACGAGATCCTGAAAAGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCtt      >  2:544916/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTTGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTc     <  1:338661/251‑1 (MQ=255)                                                                                                                                                                                                                                             cgATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctt  >  2:283495/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTG  >  NC_000913/23891‑24376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑