Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,085	A→G	Q565Q (CAA→CAG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,085	0	A	G	97.5%	253.3 / ‑2.7	81	Q565Q (CAA→CAG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (29/50);  total (30/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01

GCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGT  >  NC_000913/23842‑24331                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtg                                                                                                                                                                                                                                                 <  1:611661/251‑3 (MQ=255)    gCATCCGCGGACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtt                                                                                                                                                                                                                                              <  2:467170/251‑6 (MQ=255)     cATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                                                                                                                                                                                                                             <  1:556541/251‑7 (MQ=255)      aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCCGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                                                                                                                                                            >  2:598877/1‑244 (MQ=255)      aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                                                                                                                                                            >  1:303450/1‑244 (MQ=255)        ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                                                                                                                                                                                                                           <  1:439697/250‑1 (MQ=255)        ccGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTggttggtt                                                                                                                                                                                                                                          <  2:601244/251‑1 (MQ=255)           cgTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGAGCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCAt                                                                                                                                                                                                                                       <  1:41098/251‑1 (MQ=255)           cgTACCCTCGAACGGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTTGTTGGTTCAt                                                                                                                                                                                                                                       <  2:269584/251‑1 (MQ=255)                    gAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GCAGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGATTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                                                                                                                                                              >  1:637616/1‑251 (MQ=255)                      aCTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTa                                                                                                                                                                                                                             >  1:677099/1‑250 (MQ=255)                         gATGGAAGAAGTGGCAAA‑CGCCGTTGAAGTGAACGGAATCCAGGCGTGGGGGGGTATCGATGCTAAAGAGATCCTCGGGGACGAAGCTGACTAGTATGTGAAAGTGCCGGATACCCTC‑GACGTAGGGTTTGACTTCGGATCTACCCACTCTGCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGGACCGTGGTTGGTTCATGTCTACCCTAATGa                                                                                                                                                                                                                         <  2:602812/251‑1 (MQ=255)                                 aagTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCTCTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTccgcc                                                                                                                                                                                                                 <  2:57343/251‑4 (MQ=255)                                 aagTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCACGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAcc                                                                                                                                                                                                                 <  1:552948/251‑1 (MQ=255)                                  agTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTaa                                                                                                                                                                                                                            >  1:257939/1‑239 (MQ=255)                                  agTGGCAAAACGC‑GGTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCGTCCCTaa                                                                                                                                                                                                                            <  2:257939/239‑1 (MQ=255)                                                   gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTcc                                                                                                                                                                                                                                >  2:674930/10‑219 (MQ=255)                                                   gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTcc                                                                                                                                                                                                                                <  1:674930/210‑1 (MQ=255)                                                   gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgc                                                                                                                                                                                                <  1:456145/242‑1 (MQ=255)                                                    acgttaacggCATTCAGGCGCGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGTCTAAGCTGACCATTATGTGAAAGTGCCGGATTCTCTC‑GACGTATTGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTTCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGTCTCTGACCAGCTCCGTGGTTGGTACATTTCGTACCCAATGCTCTCCACCGCGATGaaaggcttagcg                                                                                                                                                                                               <  2:110161/243‑11 (MQ=255)                                                     agttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgcgc                                                                                                                                                                                              <  1:237181/244‑1 (MQ=255)                                                      gttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTACCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                                                                                                                                             <  1:616359/245‑1 (MQ=255)                                                      gttaacggCATCCAGGAGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                                                                                                                                             <  1:396483/245‑1 (MQ=255)                                                           cgtcagccAGGCGGGGTGGGAGTACGATGCGAAAGAGATCATCGGCGACGAAGCGGACCAGGTTGAGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAACTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTGTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCTGTAta                                                                                                                                                                                        <  2:224144/245‑2 (MQ=255)                                                            ggCATCCAGTCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                                                                                                                                                     <  2:510517/205‑1 (MQ=255)                                                            ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                                                                                                                                                     >  1:510517/1‑205 (MQ=255)                                                               aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTcc                                                                                                                                                                                    >  2:286809/1‑250 (MQ=255)                                                                    ggCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTAc                                                                                                                                                                               <  1:364926/251‑1 (MQ=255)                                                                     gCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACt                                                                                                                                                                              <  1:669755/251‑1 (MQ=255)                                                                                   cGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCtgt                                                                                                                                                                 <  2:153842/250‑3 (MQ=255)                                                                                    gATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAc                                                                                                                                                               <  1:156568/251‑1 (MQ=255)                                                                                     aTGCGAAAGAGATCCTCGACGACGAAGCTGACCAGTATGTGAAAGTGACGGATACTCTC‑GACGTATGGTTTGACTCCGGATTTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGCGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCCACGTATCGTCAGGTACTGAccccaggcattaac                                                                                                                                                              >  2:432810/1‑240 (MQ=255)                                                                                       gCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCgg                                                                                                                                                            >  2:203035/1‑250 (MQ=255)                                                                                              agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGACCCACGGCTTTACCGTGGATggg                                                                                                                                                     >  2:343959/1‑250 (MQ=255)                                                                                                          acgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAg                                                                                                                                         <  1:185067/251‑1 (MQ=255)                                                                                                           cgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                                                                                                                                                       <  1:325679/220‑1 (MQ=255)                                                                                                               gCTGACCAGTATGTGAAAGTGCCGGATTC‑TCGCGATGTATGTTTTGACTCCGGAGCTAACCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc                                                                                                                                    <  2:307374/251‑1 (MQ=255)                                                                                                               gCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc                                                                                                                                    <  1:286809/251‑1 (MQ=255)                                                                                                                      tgcatgTGAAAGTGCCGGATACTCTA‑GACGTATGGATTGCCTCCGGATCTACCCACTCTTCCGTTGTTGACATGCGTCCGGAATTTGAAGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCCCCGTGGTTGGTTCATGTCTTACCTAATGATCTCCACCGCGAAGAAAGGCAAAGCGACGTATCGTCAGGTACTGCCACACGGCTTTCACGTGGATAGTCAGGGcagacggacggataatacc                                                                                                                             >  2:620013/6‑233 (MQ=255)                                                                                                                         atgTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAt                                                                                                                           <  1:252532/248‑1 (MQ=255)                                                                                                                                aagtgcccgatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATGGACAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                    >  2:201896/21‑250 (MQ=255)                                                                                                                                    gccgtatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGGTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCg                                                                                                                <  1:496008/235‑1 (MQ=255)                                                                                                                                      cggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGtt                                                                                                              <  1:195839/237‑1 (MQ=255)                                                                                                                                           actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTcgcc                                                                                                         <  1:160846/242‑1 (MQ=255)                                                                                                                                            ctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaa                                                                                                                     >  1:614310/9‑238 (MQ=255)                                                                                                                                            ctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaa                                                                                                                     <  2:614310/230‑1 (MQ=255)                                                                                                                                                      aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                               >  1:592433/1‑250 (MQ=255)                                                                                                                                                       tGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAACGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTAGCCGCAGGAttggat                                                                                             >  2:373201/1‑246 (MQ=255)                                                                                                                                                         gTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGCGTCAGGCATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATATCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGTCTTTACCGTGGATGGGCAGAGCCCGAAGTTGTCTAAATCCATCGGCAAGCCCTGTTCGACGCAtgatgtgatg                                                                                            >  2:345344/1‑250 (MQ=255)                                                                                                                                                             gaccccGGATCTGCCCACTCTTCTGTTGGTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaa                                                                                       <  2:369569/247‑1 (MQ=255)                                                                                                                                                             gACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGTTTCGCCGCAGGATGTGATGAACa                                                                                        >  1:500826/1‑250 (MQ=255)                                                                                                                                                                    gATCTACCCACTCTTCTGTTGTTGCCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTACATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACCAAATGGgc                                                                                >  2:675758/1‑251 (MQ=255)                                                                                                                                                                     aTCTACACACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCCACGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTAAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCCAGATGTCTAAATCCATCGGCAAACCCGTTTCGCCGCAGGATGTGATGACCAAACTGGgcg                                                                               >  2:430337/1‑251 (MQ=255)                                                                                                                                                                      tCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGATGAACAACCTGGgcgc                                                                              >  2:440629/1‑251 (MQ=255)                                                                                                                                                                      tCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAAGGCTTTCCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGATGAACAAACTGGgcgc                                                                              >  2:361637/1‑251 (MQ=255)                                                                                                                                                                        tACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCgg                                                                            >  1:158639/1‑251 (MQ=255)                                                                                                                                                                         aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCCGGATGTGGTGAACAAACTGGGCGCgg                                                                            >  2:490554/1‑250 (MQ=255)                                                                                                                                                                         aCCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGgc                                                                                <  1:30757/246‑1 (MQ=255)                                                                                                                                                                                cttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTc                                                                      <  2:677099/249‑1 (MQ=255)                                                                                                                                                                                 ttctGTTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAACAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGAGCgggaattcttg                                                                   >  2:21700/1‑242 (MQ=255)                                                                                                                                                                                 ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTg                                                                    <  2:498938/250‑1 (MQ=255)                                                                                                                                                                                  tctGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCTTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGTCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCt                                                                     <  1:77068/248‑1 (MQ=255)                                                                                                                                                                                     gttgttGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTc                                                                <  1:98114/250‑1 (MQ=255)                                                                                                                                                                                     gttgttGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCt                                                               <  1:374737/251‑1 (MQ=255)                                                                                                                                                                                        gttgACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg                                                            <  1:194626/251‑1 (MQ=255)                                                                                                                                                                                           gACGTGCGTCCGGAATTTGCCGGTCAAGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCACTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTAAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCACGATGTCTAAATCCATCGGCACCACCGTTTCTCCGCAGGCTGGGATGAACAAACTGGGCGAGGATATTCTGCGTCTgtgggg                                                         >  2:616111/1‑250 (MQ=255)                                                                                                                                                                                           gACGTACGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCTACGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGGTGGTCAGGGCCGCAAGATGTCTAAATCCCTAGGCAATACCGTTTAGCCGGAGGATGTGCTTAACAAACTGGGCGCGGTAATTCTGGGCCTgtgggg                                                         >  2:367873/1‑250 (MQ=255)                                                                                                                                                                                             agggtgttcGGAATTTGCCTGTCTGGCCGCGGACATGTATATGGAAGGTACGGACCAGCCACGTGGGTGGTTCATGTCGTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTAGCGTCAGGTACTGACCCACGGTTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgttggtgg                                                       <  2:579914/243‑1 (MQ=255)                                                                                                                                                                                                                ggTCACGCAGCTGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCa                                                                                                                                                                        >  1:656861/1‑119 (MQ=255)                                                                                                                                                                                                                 gTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGt                                   <  1:626121/251‑1 (MQ=255)                                                                                                                                                                                                                       caggtgACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCTTGTCGTCCTTAATGATCTCCCCCGCGATGAAAGGCCAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGGGGATGGTCAGGGTCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTTGGCGCGGATATTCTGCGTCTGTGGTTGGCATCAGCCGCCTACACCGGTGAAATg                             <  2:262522/246‑1 (MQ=255)                                                                                                                                                                                                                         gCGGAAATGGATCTGGAAGGTGCTGACCAGCACCGTGGTTGTTTCATTTCTGCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACACCGTTGCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGc                           <  2:320650/251‑1 (MQ=255)                                                                                                                                                                                                                          gggACATGTATCTGGAAGGTTCTGACCTGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACTCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                          <  2:453286/250‑1 (MQ=255)                                                                                                                                                                                                                          cGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                          <  1:590565/251‑1 (MQ=255)                                                                                                                                                                                                                           ggACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCg                         <  1:343959/251‑1 (MQ=255)                                                                                                                                                                                                                            gACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGt                        >  1:504493/1‑251 (MQ=255)                                                                                                                                                                                                                                tGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTACTGACCCACGGCTTTACCGGGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACAACCACGGGGAAATAGCCGTTTCt                    >  2:622574/1‑251 (MQ=255)                                                                                                                                                                                                                                    tctgcaacgTTCTGACCAGCACCGGGGTTTGTTCATTTCTTCCCTAATGACCTCCACCGCGATGGAAGGCAAAGCGCCGTGTCGTCAGGTACTGACCCACGGCTTTAGCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCACCGGCAATACCTTTCCGCCTCAGGATGGGATCAACAAGCGGGGGTCGGAGAGGCTACGTCTGTGGGGGGGATCAATGGACTCCACCGGGGAAAtcgtcgggtctgac                 <  2:273574/242‑14 (MQ=255)                                                                                                                                                                                                                                            gTTCTGGCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTg        <  1:370772/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt  <  1:296877/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt  <  1:367873/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGT  >  NC_000913/23842‑24331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑