Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,983	G→C	L531F (TTG→TTC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,983	0	G	C	98.8%	270.2 / NA	86	L531L (TTG→CTC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (36/48);  minor base A (1/0);  total (37/49)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACAT‑TG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAT  >  NC_000913/23748‑24210                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGAGCCGGATACTC‑TC‑GACGTATGGTTTGAc                                                                                                                                                                                                                      >  1:413151/1‑251 (MQ=255) aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCCGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGAc                                                                                                                                                                                                                      <  1:663810/251‑1 (MQ=255)     gctGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGAGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GCCGTATGGTTTGACTCCg                                                                                                                                                                                                                  >  2:98114/3‑251 (MQ=255)      aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTATGAGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCgg                                                                                                                                                                                                                 >  2:669755/1‑251 (MQ=255)      aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCgg                                                                                                                                                                                                                 <  1:181708/251‑1 (MQ=255)        ggTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAAGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTC‑TC‑GACGAGTGGTTTGACTCCGGAt                                                                                                                                                                                                               >  2:374737/1‑251 (MQ=255)         gTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTCCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACTC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATc                                                                                                                                                                                                              <  1:108012/251‑1 (MQ=255)         gTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATc                                                                                                                                                                                                              <  1:472585/251‑1 (MQ=255)              tAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTAccc                                                                                                                                                                                                         >  1:498938/1‑251 (MQ=255)                   gTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCActct                                                                                                                                                                                                    >  1:453248/1‑251 (MQ=255)                       tGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtg                                                                                                                                                                                                >  1:235620/1‑251 (MQ=255)                            ggggtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTCCTgttgtt                                                                                                                                                                                           <  2:543306/248‑1 (MQ=255)                            ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgttgt                                                                                                                                                                                            <  2:153476/250‑1 (MQ=255)                               gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCt                                                                                                                                                                                                             >  2:69857/1‑230 (MQ=255)                               gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCt                                                                                                                                                                                                             <  1:69857/230‑1 (MQ=255)                                 aTCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAGCGGCATCCAGGCGTGGTGGGATCGCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACg                                                                                                                                                                                       <  1:330675/250‑1 (MQ=255)                                     cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt                                                                                                                                                                                  >  1:181869/1‑251 (MQ=255)                                     cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCCGCATCCGCGTGCCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGTAAGAGATCCTCGGCGTCGAAGTTGACCAGTATGTGAAAGTTCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTg                                                                                                                                                                                     <  2:339751/248‑1 (MQ=255)                                        gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                                                                                               <  2:164632/251‑1 (MQ=255)                                        gTCAGCGAACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                                                                                               >  1:273574/1‑251 (MQ=255)                                           aGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGa                                                                                                                                                                             >  1:453286/1‑250 (MQ=255)                                            gcgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAt                                                                                                                                                                           <  1:154890/251‑1 (MQ=255)                                                aCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGc                                                                                                                                                                       >  1:74726/1‑251 (MQ=255)                                                aCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGAGTCCGGAATTTGc                                                                                                                                                                       >  2:30757/1‑251 (MQ=255)                                                     gagcgtACCGATGTCACTGATCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGACAAAACGC‑GTTGAAGTTACCGGCATCCAGGCGTGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGATTTGACTCCGGTTCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTc                                                                                                                                                                  >  2:194626/5‑251 (MQ=255)                                                      gtcgtACCGATGTCACTGTGCGGGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTTAAATTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTTTTGACGTGCGTCCGGAATTTGCCGGTCa                                                                                                                                                                 <  2:71115/248‑1 (MQ=255)                                                        cgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTTCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGCTGCGAAATAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                                                                                               <  2:301468/250‑1 (MQ=255)                                                        cgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                                                                                               <  1:251089/250‑1 (MQ=255)                                                            ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGc                                                                                                                                                           >  1:307374/1‑251 (MQ=255)                                                             cGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                                                                                                                                          >  1:320650/1‑251 (MQ=255)                                                                  tCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTACCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                                                                                                                               >  2:279473/1‑209 (MQ=255)                                                                  tCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                                                                                                                               <  1:279473/209‑1 (MQ=255)                                                                   cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                                                                                                                                     <  1:404262/250‑1 (MQ=255)                                                                      tGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAt                                                                                                                                                 >  1:601244/1‑251 (MQ=255)                                                                       ggtCGTGAACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATTGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTAGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTACTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATc                                                                                                                                                <  2:534998/249‑1 (MQ=255)                                                                       gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAt                                                                                                                                                 <  1:244736/250‑1 (MQ=255)                                                                          cGTGCACAAAGACACGGAAGTGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGGATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTg                                                                                                                                              <  2:570834/250‑1 (MQ=255)                                                                           gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGACACGCAGCCGGCATGTATCTgg                                                                                                                                             >  2:626121/1‑250 (MQ=255)                                                                                caAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGtt                                                                                                                                       <  2:178652/251‑1 (MQ=255)                                                                                caAAGAAACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCATGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAACCTGACCAGTATGTGAAAGTGACGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGGCGTGCGTCCGGAATTTGCCGGCCACGCAGCGGACATGTATCTGGAAGGtt                                                                                                                                       >  2:18447/1‑251 (MQ=255)                                                                                  aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                                                                                                                                     <  1:206098/251‑1 (MQ=255)                                                                                  aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                                                                                                                                     >  1:452131/1‑251 (MQ=255)                                                                                       acGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc                                                                                                                                 >  1:72595/1‑250 (MQ=255)                                                                                        cGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGACGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc                                                                                                                                 >  1:96624/1‑249 (MQ=255)                                                                                               gCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtg                                                                                                                        <  1:611661/251‑3 (MQ=255)                                                                                                cTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGTGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑ATCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTATTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGACCCTCAGCGGACATGGATCTGGAAGGTTATGACCagcaacgtgg                                                                                                                       >  1:239085/1‑242 (MQ=255)                                                                                                  gCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGC‑GTTGCAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGGCGAAGCTGCACAGTATGTGAAAGTGCCGGATACTA‑TC‑GACGTATGGTATGACTCCGGATCTACCCACTCTTCTGTTGTTGACATGCGTCCGGAATTTGCCGGTCAAGTCGCGGCCGTGTATCAGGAAGGTTCTGTCCagaaccgtggat                                                                                                                     >  2:175161/1‑240 (MQ=255)                                                                                                  gCATCCGCGGACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtt                                                                                                                     <  2:467170/251‑6 (MQ=255)                                                                                                   cATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                                                                                                    <  1:556541/251‑7 (MQ=255)                                                                                                    aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCCGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                                   >  2:598877/1‑244 (MQ=255)                                                                                                    aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                                   >  1:303450/1‑244 (MQ=255)                                                                                                      ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                                                                                                  <  1:439697/250‑1 (MQ=255)                                                                                                      ccGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTggttggtt                                                                                                                 <  2:601244/251‑1 (MQ=255)                                                                                                         cgTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGAGCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCAt                                                                                                              <  1:41098/251‑1 (MQ=255)                                                                                                         cgTACCCTCGAACGGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTTGTTGGTTCAt                                                                                                              <  2:269584/251‑1 (MQ=255)                                                                                                                  gAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GCAGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGATTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                                     >  1:637616/1‑251 (MQ=255)                                                                                                                    aCTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTa                                                                                                    >  1:677099/1‑250 (MQ=255)                                                                                                                       gATGGAAGAAGTGGCAAA‑CGCCGTTGAAGTGAACGGAATCCAGGCGTGGGGGGGTATCGATGCTAAAGAGATCCTCGGGGACGAAGCTGACTAGTATGTGAAAGTGCCGGATACCC‑TC‑GACGTAGGGTTTGACTTCGGATCTACCCACTCTGCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGGACCGTGGTTGGTTCATGTCTACCCTAATGa                                                                                                <  2:602812/251‑1 (MQ=255)                                                                                                                               aagTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCTCTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTccgcc                                                                                        <  2:57343/251‑4 (MQ=255)                                                                                                                               aagTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCACGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAcc                                                                                        <  1:552948/251‑1 (MQ=255)                                                                                                                                agTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTaa                                                                                                   >  1:257939/1‑239 (MQ=255)                                                                                                                                agTGGCAAAACGC‑GGTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCGTCCCTaa                                                                                                   <  2:257939/239‑1 (MQ=255)                                                                                                                                                 gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTcc                                                                                                       >  2:674930/10‑219 (MQ=255)                                                                                                                                                 gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTcc                                                                                                       <  1:674930/210‑1 (MQ=255)                                                                                                                                                 gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgc                                                                       <  1:456145/242‑1 (MQ=255)                                                                                                                                                  acgttaacggCATTCAGGCGCGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGTCTAAGCTGACCATTATGTGAAAGTGCCGGATTCTC‑TC‑GACGTATTGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTTCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGTCTCTGACCAGCTCCGTGGTTGGTACATTTCGTACCCAATGCTCTCCACCGCGATGaaaggcttagcg                                                                      <  2:110161/243‑11 (MQ=255)                                                                                                                                                   agttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgcgc                                                                     <  1:237181/244‑1 (MQ=255)                                                                                                                                                    gttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTACCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                    <  1:616359/245‑1 (MQ=255)                                                                                                                                                    gttaacggCATCCAGGAGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                    <  1:396483/245‑1 (MQ=255)                                                                                                                                                         cgtcagccAGGCGGGGTGGGAGTACGATGCGAAAGAGATCATCGGCGACGAAGCGGACCAGGTTGAGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAACTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTGTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCTGTAta                                                               <  2:224144/245‑2 (MQ=255)                                                                                                                                                          ggCATCCAGTCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                            <  2:510517/205‑1 (MQ=255)                                                                                                                                                          ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                            >  1:510517/1‑205 (MQ=255)                                                                                                                                                             aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTcc                                                           >  2:286809/1‑250 (MQ=255)                                                                                                                                                                  ggCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTAc                                                      <  1:364926/251‑1 (MQ=255)                                                                                                                                                                   gCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACt                                                     <  1:669755/251‑1 (MQ=255)                                                                                                                                                                                 cGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCtgt                                        <  2:153842/250‑3 (MQ=255)                                                                                                                                                                                  gATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAc                                      <  1:156568/251‑1 (MQ=255)                                                                                                                                                                                   aTGCGAAAGAGATCCTCGACGACGAAGCTGACCAGTATGTGAAAGTGACGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATTTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGCGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCCACGTATCGTCAGGTACTGAccccaggcattaac                                     >  2:432810/1‑240 (MQ=255)                                                                                                                                                                                     gCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCgg                                   >  2:203035/1‑250 (MQ=255)                                                                                                                                                                                            agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGACCCACGGCTTTACCGTGGATggg                            >  2:343959/1‑250 (MQ=255)                                                                                                                                                                                                        acgaAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAg                <  1:185067/251‑1 (MQ=255)                                                                                                                                                                                                         cgaAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                              <  1:325679/220‑1 (MQ=255)                                                                                                                                                                                                             gCTGACCAGTATGTGAAAGTGCCGGATTC‑T‑CGCGATGTATGTTTTGACTCCGGAGCTAACCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc           <  2:307374/251‑1 (MQ=255)                                                                                                                                                                                                             gCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc           <  1:286809/251‑1 (MQ=255)                                                                                                                                                                                                                    tgcatgTGAAAGTGCCGGATACTC‑TA‑GACGTATGGATTGCCTCCGGATCTACCCACTCTTCCGTTGTTGACATGCGTCCGGAATTTGAAGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCCCCGTGGTTGGTTCATGTCTTACCTAATGATCTCCACCGCGAAGAAAGGCAAAGCGACGTATCGTCAGGTACTGCCACACGGCTTTCACGTGGATAGTCAGGGcagacggacggataatacc    >  2:620013/6‑233 (MQ=255)                                                                                                                                                                                                                       atgTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAt  <  1:252532/248‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     AGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACAT‑TG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAT  >  NC_000913/23748‑24210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑