BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,956	T→C	D522D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,956	0	T	C	99.0%	330.4 / NA	101	D522D (GAT→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (43/57); minor base A (1/0); total (44/57)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 101 total aligned reads displayed.

CGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACAT‑TG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTC  >  NC_000913/23710‑24201

cGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGt                                                                                                                                                                                                                                                     >  1:518598/1‑251 (MQ=255)

  tgacgtggtgcccGGACGTGGGCCAGGCGCGTATCGAGTCGATGGTTTCTAACCGTCCTGACGGGTGTTTCTCCCGTCAGCGCACCTGGGGCGTACCGATGGCACGGTTCGCGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAATTGATGGAAGAAGTGGTAAAACGC‑GTTGAAGTTAACGGCATCTAGGCGTGGTGGGATCTCGGTGCGAACGAGATCCTCGGCGACGAAGCTGACCAGTa                                                                                                                                                                                                                                                    <  2:534770/240‑1 (MQ=255)

  tGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGttt                                                                                                                                                                                                                                                   >  1:486598/1‑249 (MQ=255)

     aGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTAtgtg                                                                                                                                                                                                                                                <  1:592928/251‑1 (MQ=255)

        ggATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGaaa                                                                                                                                                                                                                                             <  1:392200/251‑1 (MQ=255)

           ccccGGAATGGGGCCAGGCTGGTTGCGTGGCGATGGTTGCTAACCGTACTGACTGGTGTATCCCTCGTCATCGCACCAGGGGCCGACCGATGTCTCTGGTCGTGCATAAAGACGCGGAAGAGCCGCATCCGCGTACCCTCGACCTGATGGAAGAAGTGGCAATGCGC‑GTTGAAGTTAACGGCATGCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGTCGACGAAGCTGTCCAGTATGTGAAAGTg                                                                                                                                                                                                                                          <  2:315340/250‑1 (MQ=255)

              cGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCg                                                                                                                                                                                                                                       >  1:664338/1‑251 (MQ=255)

               ggACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGACGCTGACCCGTATGTGAAAGTGCCgg                                                                                                                                                                                                                                      >  2:456145/1‑251 (MQ=255)

                gACTGGGGCCAGGCGCGGATCGAGTCGATGGTTGCTAACCGTCCCGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGa                                                                                                                                                                                                                                     <  2:307805/251‑1 (MQ=255)

                   tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                                                                                                                                                                                  >  1:543306/1‑248 (MQ=255)

                        ccagggtcgTATCGGGGCGATGGGTGCTAACCGTCCTGACTGGGGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactcgcg                                                                                                                                                                                                                             <  2:389756/244‑9 (MQ=255)

                          aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                                                                                           <  1:569403/251‑11 (MQ=255)

                                  aTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCATCGGCGACGAAGCTGACCAGTATGTGAAAGTGGCGGagactctcgacgaatggtt                                                                                                                                                                                                                   >  2:296877/1‑233 (MQ=255)

                                    cGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggttt                                                                                                                                                                                                                  <  1:183893/250‑20 (MQ=255)

                                      aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGCAGTTAACGGCATCCCGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgccgtatggtttgac                                                                                                                                                                                                               >  2:251089/1‑229 (MQ=255)

                                      aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGAGCCGGATACTC‑TC‑GACGTATGGTTTGAc                                                                                                                                                                                                             >  1:413151/1‑251 (MQ=255)

                                      aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCCGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGAc                                                                                                                                                                                                             <  1:663810/251‑1 (MQ=255)

                                           aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTATGAGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCgg                                                                                                                                                                                                        >  2:669755/1‑251 (MQ=255)

                                           aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCgg                                                                                                                                                                                                        <  1:181708/251‑1 (MQ=255)

                                             ggTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAAGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTC‑TC‑GACGAGTGGTTTGACTCCGGAt                                                                                                                                                                                                      >  2:374737/1‑251 (MQ=255)

                                              gTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTCCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACTC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATc                                                                                                                                                                                                     <  1:108012/251‑1 (MQ=255)

                                              gTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATc                                                                                                                                                                                                     <  1:472585/251‑1 (MQ=255)

                                                   tAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTAccc                                                                                                                                                                                                >  1:498938/1‑251 (MQ=255)

                                                        gTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCActct                                                                                                                                                                                           >  1:453248/1‑251 (MQ=255)

                                                            tGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtg                                                                                                                                                                                       >  1:235620/1‑251 (MQ=255)

                                                                 ggggtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTCCTgttgtt                                                                                                                                                                                  <  2:543306/248‑1 (MQ=255)

                                                                 ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgttgt                                                                                                                                                                                   <  2:153476/250‑1 (MQ=255)

                                                                    gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCt                                                                                                                                                                                                    >  2:69857/1‑230 (MQ=255)

                                                                    gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCt                                                                                                                                                                                                    <  1:69857/230‑1 (MQ=255)

                                                                      aTCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAGCGGCATCCAGGCGTGGTGGGATCGCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACg                                                                                                                                                                              <  1:330675/250‑1 (MQ=255)

                                                                          cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt                                                                                                                                                                         >  1:181869/1‑251 (MQ=255)

                                                                          cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCCGCATCCGCGTGCCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGTAAGAGATCCTCGGCGTCGAAGTTGACCAGTATGTGAAAGTTCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTg                                                                                                                                                                            <  2:339751/248‑1 (MQ=255)

                                                                             gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                                                                                      <  2:164632/251‑1 (MQ=255)

                                                                             gTCAGCGAACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                                                                                      >  1:273574/1‑251 (MQ=255)

                                                                                aGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGa                                                                                                                                                                    >  1:453286/1‑250 (MQ=255)

                                                                                 gcgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAt                                                                                                                                                                  <  1:154890/251‑1 (MQ=255)

                                                                                     aCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGc                                                                                                                                                              >  1:74726/1‑251 (MQ=255)

                                                                                     aCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGAGTCCGGAATTTGc                                                                                                                                                              >  2:30757/1‑251 (MQ=255)

                                                                                          gagcgtACCGATGTCACTGATCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGACAAAACGC‑GTTGAAGTTACCGGCATCCAGGCGTGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGATTTGACTCCGGTTCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTc                                                                                                                                                         >  2:194626/5‑251 (MQ=255)

                                                                                           gtcgtACCGATGTCACTGTGCGGGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTTAAATTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTTTTGACGTGCGTCCGGAATTTGCCGGTCa                                                                                                                                                        <  2:71115/248‑1 (MQ=255)

                                                                                             cgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTTCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGCTGCGAAATAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                                                                                      <  2:301468/250‑1 (MQ=255)

                                                                                             cgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                                                                                      <  1:251089/250‑1 (MQ=255)

                                                                                                 ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGc                                                                                                                                                  >  1:307374/1‑251 (MQ=255)

                                                                                                  cGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                                                                                                                                 >  1:320650/1‑251 (MQ=255)

                                                                                                       tCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTACCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                                                                                                                      >  2:279473/1‑209 (MQ=255)

                                                                                                       tCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                                                                                                                      <  1:279473/209‑1 (MQ=255)

                                                                                                        cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                                                                                                                            <  1:404262/250‑1 (MQ=255)

                                                                                                           tGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAt                                                                                                                                        >  1:601244/1‑251 (MQ=255)

                                                                                                            ggtCGTGAACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATTGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTAGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTACTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATc                                                                                                                                       <  2:534998/249‑1 (MQ=255)

                                                                                                            gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAt                                                                                                                                        <  1:244736/250‑1 (MQ=255)

                                                                                                               cGTGCACAAAGACACGGAAGTGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGGATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTg                                                                                                                                     <  2:570834/250‑1 (MQ=255)

                                                                                                                gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGACACGCAGCCGGCATGTATCTgg                                                                                                                                    >  2:626121/1‑250 (MQ=255)

                                                                                                                     caAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGtt                                                                                                                              <  2:178652/251‑1 (MQ=255)

                                                                                                                     caAAGAAACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCATGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAACCTGACCAGTATGTGAAAGTGACGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGGCGTGCGTCCGGAATTTGCCGGCCACGCAGCGGACATGTATCTGGAAGGtt                                                                                                                              >  2:18447/1‑251 (MQ=255)

                                                                                                                      aacgACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                                                                                                                        >  2:26691/4‑146 (MQ=255)

                                                                                                                      aaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACTGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                                                                                                                        <  1:26691/146‑1 (MQ=255)

                                                                                                                       aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                                                                                                                            >  1:452131/1‑251 (MQ=255)

                                                                                                                       aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                                                                                                                            <  1:206098/251‑1 (MQ=255)

                                                                                                                            acGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc                                                                                                                        >  1:72595/1‑250 (MQ=255)

                                                                                                                             cGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGACGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc                                                                                                                        >  1:96624/1‑249 (MQ=255)

                                                                                                                                    gCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtg                                                                                                               <  1:611661/251‑3 (MQ=255)

                                                                                                                                     cTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGTGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATA‑ATCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTATTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGACCCTCAGCGGACATGGATCTGGAAGGTTATGACCagcaacgtgg                                                                                                              >  1:239085/1‑242 (MQ=255)

                                                                                                                                       gCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGC‑GTTGCAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGGCGAAGCTGCACAGTATGTGAAAGTGCCGGATACTA‑TC‑GACGTATGGTATGACTCCGGATCTACCCACTCTTCTGTTGTTGACATGCGTCCGGAATTTGCCGGTCAAGTCGCGGCCGTGTATCAGGAAGGTTCTGTCCagaaccgtggat                                                                                                            >  2:175161/1‑240 (MQ=255)

                                                                                                                                       gCATCCGCGGACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtt                                                                                                            <  2:467170/251‑6 (MQ=255)

                                                                                                                                        cATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                                                                                           <  1:556541/251‑7 (MQ=255)

                                                                                                                                         aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCCGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                          >  2:598877/1‑244 (MQ=255)

                                                                                                                                         aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                          >  1:303450/1‑244 (MQ=255)

                                                                                                                                           ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                                                                                         <  1:439697/250‑1 (MQ=255)

                                                                                                                                           ccGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTggttggtt                                                                                                        <  2:601244/251‑1 (MQ=255)

                                                                                                                                              cgTACCCTCGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGAGCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCAt                                                                                                     <  1:41098/251‑1 (MQ=255)

                                                                                                                                              cgTACCCTCGAACGGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTTGTTGGTTCAt                                                                                                     <  2:269584/251‑1 (MQ=255)

                                                                                                                                                       gAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GCAGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGATTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                            >  1:637616/1‑251 (MQ=255)

                                                                                                                                                         aCTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTa                                                                                           >  1:677099/1‑250 (MQ=255)

                                                                                                                                                            gATGGAAGAAGTGGCAAA‑CGCCGTTGAAGTGAACGGAATCCAGGCGTGGGGGGGTATCGATGCTAAAGAGATCCTCGGGGACGAAGCTGACTAGTATGTGAAAGTGCCGGATACCC‑TC‑GACGTAGGGTTTGACTTCGGATCTACCCACTCTGCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGGACCGTGGTTGGTTCATGTCTACCCTAATGa                                                                                       <  2:602812/251‑1 (MQ=255)

                                                                                                                                                                    aagTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCTCTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTccgcc                                                                               <  2:57343/251‑4 (MQ=255)

                                                                                                                                                                    aagTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCACGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAcc                                                                               <  1:552948/251‑1 (MQ=255)

                                                                                                                                                                     agTGGCAAAACGC‑GTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTaa                                                                                          >  1:257939/1‑239 (MQ=255)

                                                                                                                                                                     agTGGCAAAACGC‑GGTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCGTCCCTaa                                                                                          <  2:257939/239‑1 (MQ=255)

                                                                                                                                                                                      gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTcc                                                                                              >  2:674930/10‑219 (MQ=255)

                                                                                                                                                                                      gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTcc                                                                                              <  1:674930/210‑1 (MQ=255)

                                                                                                                                                                                      gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgc                                                              <  1:456145/242‑1 (MQ=255)

                                                                                                                                                                                       acgttaacggCATTCAGGCGCGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGTCTAAGCTGACCATTATGTGAAAGTGCCGGATTCTC‑TC‑GACGTATTGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTTCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGTCTCTGACCAGCTCCGTGGTTGGTACATTTCGTACCCAATGCTCTCCACCGCGATGaaaggcttagcg                                                             <  2:110161/243‑11 (MQ=255)

                                                                                                                                                                                        agttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAgcgc                                                            <  1:237181/244‑1 (MQ=255)

                                                                                                                                                                                         gttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTACCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                           <  1:616359/245‑1 (MQ=255)

                                                                                                                                                                                         gttaacggCATCCAGGAGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                           <  1:396483/245‑1 (MQ=255)

                                                                                                                                                                                              cgtcagccAGGCGGGGTGGGAGTACGATGCGAAAGAGATCATCGGCGACGAAGCGGACCAGGTTGAGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAACTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGTGTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCTGTAta                                                      <  2:224144/245‑2 (MQ=255)

                                                                                                                                                                                               ggCATCCAGTCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                   <  2:510517/205‑1 (MQ=255)

                                                                                                                                                                                               ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                   >  1:510517/1‑205 (MQ=255)

                                                                                                                                                                                                  aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTcc                                                  >  2:286809/1‑250 (MQ=255)

                                                                                                                                                                                                       ggCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTAc                                             <  1:364926/251‑1 (MQ=255)

                                                                                                                                                                                                        gCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACt                                            <  1:669755/251‑1 (MQ=255)

                                                                                                                                                                                                                      cGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCtgt                               <  2:153842/250‑3 (MQ=255)

                                                                                                                                                                                                                       gATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAc                             <  1:156568/251‑1 (MQ=255)

                                                                                                                                                                                                                        aTGCGAAAGAGATCCTCGACGACGAAGCTGACCAGTATGTGAAAGTGACGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATTTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGCGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCCACGTATCGTCAGGTACTGAccccaggcattaac                            >  2:432810/1‑240 (MQ=255)

                                                                                                                                                                                                                          gCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCgg                          >  2:203035/1‑250 (MQ=255)

                                                                                                                                                                                                                                 agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGACCCACGGCTTTACCGTGGATggg                   >  2:343959/1‑250 (MQ=255)

                                                                                                                                                                                                                                             acgaAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAg       <  1:185067/251‑1 (MQ=255)

                                                                                                                                                                                                                                              cgaAGCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                     <  1:325679/220‑1 (MQ=255)

                                                                                                                                                                                                                                                  gCTGACCAGTATGTGAAAGTGCCGGATTC‑T‑CGCGATGTATGTTTTGACTCCGGAGCTAACCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc  <  2:307374/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  gCTGACCAGTATGTGAAAGTGCCGGATACTC‑TC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc  <  1:286809/251‑1 (MQ=255)

CGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGC‑GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACAT‑TG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTC  >  NC_000913/23710‑24201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`