BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,803	T→C	G471G (GGT→GGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,803	0	T	C	100.0%	383.1 / NA	119	G471G (GGT→GGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (59/60); total (59/60)

Only 100 of 119 total aligned reads displayed.

AAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGC  >  NC_000913/23557‑24045

aaaaGGCGCGCTGCTGCACGTTGAGAAAATGCCGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGCAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGCCTGGTGGATCTCCCGTAAGCGCACCTTGGGCGTAc                                                                                                                                                                                                                                                 >  2:370772/1‑251 (MQ=255)

 aaaGGCGCGCTGCTGCTCGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGGCAGCGCACCTGGGGCGTAcc                                                                                                                                                                                                                                                <  1:623321/251‑1 (MQ=255)

 aaaGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTAcc                                                                                                                                                                                                                                                <  1:284253/251‑1 (MQ=255)

   aGGCGCGCTGCTGCACGTTGAGAAAATGCAGCCCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACTTGGGGCGTACCGa                                                                                                                                                                                                                                              <  2:339763/251‑1 (MQ=255)

    ggCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGAt                                                                                                                                                                                                                                             <  1:324750/251‑1 (MQ=255)

      cgcgcTGCGGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAAGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGt                                                                                                                                                                                                                                           <  2:259523/251‑1 (MQ=255)

           tgctgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTg                                                                                                                                                                                                                                      >  1:110161/1‑251 (MQ=255)

            gctgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGt                                                                                                                                                                                                                                     >  1:671084/1‑251 (MQ=255)

            gcggcACGTTGAGAAAAGGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGt                                                                                                                                                                                                                                     <  1:150419/248‑1 (MQ=255)

                        gaAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAACCGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTCGTCAGCATGGCTCAGAAAGTTCTGCGTGCGCAGTCACTGAAAGAGATCAACGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTACTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGCTGTCCCTGTTCGTGCCC‑aaa                                                                                                                                                                                                                         >  2:305206/1‑250 (MQ=255)

                         aaaaTGCAGCACAGCTATCCGTGCTGCGGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGGGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGTTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGa                                                                                                                                                                                                                       <  1:59569/251‑1 (MQ=255)

                           atggccgcccaGCTATCCGTTCTGCGGGCGTCACAAAACGCCGATCATCTTCCGCGCGTCGCCGCAGTGGTTCGTCAGCATGGATCAGAATGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGTTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGaca                                                                                                                                                                                                                     <  2:658058/242‑1 (MQ=255)

                           aaTGCAGCACAGCTATCCGTGCTGCTGGAGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGac                                                                                                                                                                                                                      >  1:579914/1‑250 (MQ=255)

                                 gctcaGCTATCCGGGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAag                                                                                                                                                                                                               <  2:21564/248‑1 (MQ=255)

                                 gcaCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGa                                                                                                                                                                                                                 >  1:383735/1‑249 (MQ=255)

                                 gcaCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCGGCCGAGCGACGCCGCAGTGGTTCGTCATCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGTATCCCGGACTGGGGCCAGGAGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAag                                                                                                                                                                                                               <  2:537973/251‑1 (MQ=255)

                                   acaGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCCTCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGCTCAGAAAGGTCTGCGTGCGCAGTCACTGAACGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCCCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGTGCAC‑AAAGACACGGAag                                                                                                                                                                                                               >  2:307494/1‑249 (MQ=255)

                                   acaGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAagag                                                                                                                                                                                                             <  1:129474/251‑1 (MQ=255)

                                   acaGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAag                                                                                                                                                                                                               >  1:63588/1‑249 (MQ=255)

                                             gTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGCCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTGTCTCCCGTCAGCGCACCTGGGGCGTACCGCTGTCACTGTTCGTGCAC‑AAGGACACGGAAGAGCTGCATCcgc                                                                                                                                                                                                   >  2:237181/1‑251 (MQ=255)

                                             gTGCTGCAGGCGTCACCAAACGCCGATCCTCTTCAGCGCGACGACGCAGTGACTCGTCAGCATGGCTCAGATAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAATGGCGTGAAGTGGATCCCGGACTGGGGCCAGGCGCGGATCGAGTCGATGGTTGCTAACCGCCCTGATTGGTGTCTCTCCCGTCAGCCCACATGGGGCGTACCGATGACCCTGTTCGTGCAC‑AAAGACACggccaagctgcctcagc                                                                                                                                                                                                   >  2:330675/1‑236 (MQ=255)

                                                  gctgGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAATAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTAc                                                                                                                                                                                               <  1:639018/250‑1 (MQ=255)

                                                    tgGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATg                                                                                                                                                                                                                                            >  1:75172/1‑204 (MQ=255)

                                                    tgGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGGCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGAtt                                                                                                                                                                                                                                            <  2:75172/204‑2 (MQ=255)

                                                      gcggcacacaACGCCGCTCATCTTCCTCGCTACTCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACGTAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGGCTCGAGTCGATGGTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCTTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCtcg                                                                                                                                                                                          <  2:597259/247‑3 (MQ=255)

                                                        gTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGAAGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCtcga                                                                                                                                                                                         >  1:262522/1‑247 (MQ=255)

                                                             aaaaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGCGTCGATGGTTGCTAACCGTCCTGCCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTCCCGATGTCACTGTTCGTGCAC‑AAAGACCCGGAAGAGCTGCATCCGCGTCCCCTCGACCTGAt                                                                                                                                                                                   >  2:592928/1‑251 (MQ=255)

                                                             aaaaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGAAGGGTGCTAACCGTCCTGACTGGGGGATCTCCCGTCAGCGCACCTAGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACCCGGAAGAGCGGCATCCGCGTACCCTCGAACTGAt                                                                                                                                                                                   >  2:404262/1‑251 (MQ=255)

                                                              aaaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCCGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACCCGGACGAGCTGCATCCGCGAACCCtcgcactgcgg                                                                                                                                                                                  >  2:78160/1‑241 (MQ=255)

                                                              aaaCGCCCATTATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGGCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTTCATCCGCGTACCCTCGAACTGATg                                                                                                                                                                                  <  2:424141/251‑1 (MQ=255)

                                                               aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCCGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑ACAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTgg                                                                                                                                                                                 >  2:252532/1‑251 (MQ=255)

                                                                 cGCCGATCATCTTCAGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                >  1:542927/1‑250 (MQ=255)

                                                                    cGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaaga                                                                                                                                                                             >  1:178652/1‑250 (MQ=255)

                                                                    cGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCAGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGGCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACCCGGAAGAGCTGCATCCGCGTCCCCACGCACTGATGgaaga                                                                                                                                                                             >  2:472585/1‑250 (MQ=255)

                                                                         atcTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGc                                                                                                                                                                       >  1:153476/1‑251 (MQ=255)

                                                                             tCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCa                                                                                                                                                                      <  1:521979/248‑1 (MQ=255)

                                                                                    gACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCCGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGGGGCAAAACGCGTTg                                                                                                                                                            >  1:307805/1‑251 (MQ=255)

                                                                                    gACGCCGCAGTGGGTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTg                                                                                                                                                            <  1:305206/251‑1 (MQ=255)

                                                                                       gacgcAGTGGTTCGTCAGTATGGATCAGAAAGGTCGGCGGGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGAGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGGCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCTTTGAAg                                                                                                                                                         <  2:593716/249‑1 (MQ=255)

                                                                                          gcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGtt                                                                                                                                                       >  1:153842/1‑249 (MQ=255)

                                                                                            agtggaacGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGGATCGAGTAGATGGTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTCCCGATGTCAATGTTCG‑ACACCAAAGACCCTGAAGAGCTGCATCCGCGTCCCCTAGAACTGCTAGAAGAAGTGGCAAGAAGCGTTGAAGttac                                                                                                                                                     >  2:10809/8‑247 (MQ=255)

                                                                                            aGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttaac                                                                                                                                                    <  1:310638/251‑5 (MQ=255)

                                                                                                gTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCTCAGTCACTGAAAGAGATCAAAGGCGTGCAGTTGAGCTCGGACTGGGGCCAGGGGCGTGTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTAGCTCCCGTCATCGCACCTGGTGCGTACCTATGTCACTGTTCTTGCAC‑AAAGACACGGAAGAGCTTCATCCGCGTGCTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttaacggca                                                                                                                                                <  1:319330/251‑9 (MQ=255)

                                                                                                   cGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttaacggcatcc                                                                                                                                             >  1:369569/1‑240 (MQ=255)

                                                                                                   cGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttaacggcatc                                                                                                                                              <  1:582077/250‑11 (MQ=255)

                                                                                                       aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGc                                                                                                                                         <  2:152451/251‑1 (MQ=255)

                                                                                                         cATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCgg                                                                                                                                       >  1:467170/1‑250 (MQ=255)

                                                                                                            ggATCAGAAAGGTCTGCGTGCGCAGTCACGGAAAGATATCAAAGGCGTCAAGTGGATCCCGGACTGGGGCCCGGCGCGTATCGAGTCGATGGTTGCTAACCGTCATGACTGGTGTATCTCCCGTCAGCTCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTTCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCTGGCgtgg                                                                                                                                     <  2:365527/250‑1 (MQ=255)

                                                                                                             gATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCgtggtg                                                                                                                                   >  1:659389/1‑251 (MQ=255)

                                                                                                               tCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTggg                                                                                                                                 >  1:269584/1‑251 (MQ=255)

                                                                                                                  gAAAGGGCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGAtc                                                                                                                              <  1:362670/251‑1 (MQ=255)

                                                                                                                     agggcTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGTAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGATGTTAACGGCATCCAGGCGTGGTGGGAtcgcg                                                                                                                           <  1:595126/247‑4 (MQ=255)

                                                                                                                     aGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCg                                                                                                                           >  1:35340/1‑251 (MQ=255)

                                                                                                                       ggcTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAt                                                                                                                         <  1:453043/249‑1 (MQ=255)

                                                                                                                          tgcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGCTGCTAACCGTCCTGACTGGTGTATCTCCGGTCAGCGAACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATACGCGAACCCTAGAACTGATGGAAGCAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCg                                                                                                                      >  1:428403/1‑251 (MQ=255)

                                                                                                                               gcgcAGTCACTGGAGGATATCAAAGGCGTGCGTTGGATCGCAGACGGGGGCCAGGCGCGTATCGAGGCGAGTGTTGCTAGCCGTCCTGACTGGTGTATCTCGCGTCAGCGCACCTGGGGCGTACCGATGGCTCTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCGTCCGCGTACGCTCGAACTGATGGAAGAAGGGGCAAAACGCGGGGAAGTTAACGGCATCCAGGCGTGGTGGGAACTCGATGCGAAaga                                                                                                                 <  2:566660/251‑1 (MQ=255)

                                                                                                                               gcgcAGGCTCTGAAAGAGATTAAAGGCGTGCAGTGGATCCCGGACTGGGGCCTGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTTCCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGGTAACGGCAGCCAGGCGTGGTGGGATCTCTATGCGAAaga                                                                                                                 <  2:61884/251‑1 (MQ=255)

                                                                                                                                   aGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAag                                                                                                                  >  1:452913/1‑246 (MQ=255)

                                                                                                                                      cACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTgg                                                                                                                                                                        >  1:362426/1‑189 (MQ=255)

                                                                                                                                       aCTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCg                                                                                                         <  1:307494/251‑1 (MQ=255)

                                                                                                                                       aCTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCg                                                                                                         <  1:673989/251‑1 (MQ=255)

                                                                                                                                          gAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcg                                                                                                      >  1:534998/1‑251 (MQ=255)

                                                                                                                                          gAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcg                                                                                                      >  1:164632/1‑251 (MQ=255)

                                                                                                                                             agagATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgacg                                                                                                   >  2:181708/1‑251 (MQ=255)

                                                                                                                                                 aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                               >  1:470304/1‑251 (MQ=255)

                                                                                                                                                   cAAAGGCGTGCAGGGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGAGGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAgatg                                                                                             <  2:543912/251‑4 (MQ=255)

                                                                                                                                                    aaaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAAGCTg                                                                                             >  2:154890/1‑250 (MQ=255)

                                                                                                                                                       ggCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGacca                                                                                         <  1:446107/251‑4 (MQ=255)

                                                                                                                                                         cGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGt                                                                                       >  1:518598/1‑251 (MQ=255)

                                                                                                                                                           tgacgtggtgcccGGACGTGGGCCAGGCGCGTATCGAGTCGATGGTTTCTAACCGTCCTGACGGGTGTTTCTCCCGTCAGCGCACCTGGGGCGTACCGATGGCACGGTTCGCGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAATTGATGGAAGAAGTGGTAAAACGCGTTGAAGTTAACGGCATCTAGGCGTGGTGGGATCTCGGTGCGAACGAGATCCTCGGCGACGAAGCTGACCAGTa                                                                                      <  2:534770/240‑1 (MQ=255)

                                                                                                                                                           tGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGttt                                                                                     >  1:486598/1‑249 (MQ=255)

                                                                                                                                                              aGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTAtgtg                                                                                  <  1:592928/251‑1 (MQ=255)

                                                                                                                                                                 ggctCCCGGACTAAGGCCAGGCGCGTATCGCGTAGATGGTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTAACTGTTCGAGCAC‑AACGACACGGCAGAGCTGCATCCGAGTACCCTCGCACTGATGGCAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGAAGACGCTGacccggatgtgaa                                                                                >  2:338661/4‑238 (MQ=255)

                                                                                                                                                                    ccccGGAATGGGGCCAGGCTGGTTGCGTGGCGATGGTTGCTAACCGTACTGACTGGTGTATCCCTCGTCATCGCACCAGGGGCCGACCGATGTCTCTGGTCGTGCAT‑AAAGACGCGGAAGAGCCGCATCCGCGTACCCTCGACCTGATGGAAGAAGTGGCAATGCGCGTTGAAGTTAACGGCATGCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGTCGACGAAGCTGTCCAGTATGTGAAAGTg                                                                            <  2:315340/250‑1 (MQ=255)

                                                                                                                                                                         gACTGGGGCCAGGCGCGGATCGAGTCGATGGTTGCTAACCGTCCCGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGa                                                                       <  2:307805/251‑1 (MQ=255)

                                                                                                                                                                            tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                    >  1:543306/1‑248 (MQ=255)

                                                                                                                                                                                 ccagggtcgTATCGGGGCGATGGGTGCTAACCGTCCTGACTGGGGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactcgcg                                                               <  2:389756/244‑9 (MQ=255)

                                                                                                                                                                                           aTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCATCGGCGACGAAGCTGACCAGTATGTGAAAGTGGCGGagactctcgacgaatggtt                                                     >  2:296877/1‑233 (MQ=255)

                                                                                                                                                                                             cGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggttt                                                    <  1:183893/250‑20 (MQ=255)

                                                                                                                                                                                               aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGCAGTTAACGGCATCCCGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgccgtatggtttgac                                                 >  2:251089/1‑229 (MQ=255)

                                                                                                                                                                                               aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGAGCCGGATACTCTCGACGTATGGTTTGAc                                                 >  1:413151/1‑251 (MQ=255)

                                                                                                                                                                                               aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCCGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGAc                                                 <  1:663810/251‑1 (MQ=255)

                                                                                                                                                                                                    aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTATGAGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                            >  2:669755/1‑251 (MQ=255)

                                                                                                                                                                                                    aTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                            <  1:181708/251‑1 (MQ=255)

                                                                                                                                                                                                      ggTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAAGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTCTCGACGAGTGGTTTGACTCCGGAt                                          >  2:374737/1‑251 (MQ=255)

                                                                                                                                                                                                       gTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTCCCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACTCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATc                                         <  1:108012/251‑1 (MQ=255)

                                                                                                                                                                                                       gTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATc                                         <  1:472585/251‑1 (MQ=255)

                                                                                                                                                                                                            tAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTAccc                                    >  1:498938/1‑251 (MQ=255)

                                                                                                                                                                                                                     tGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtg                           >  1:235620/1‑251 (MQ=255)

                                                                                                                                                                                                                          ggggtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTCCTgttgtt                      <  2:543306/248‑1 (MQ=255)

                                                                                                                                                                                                                          ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgttgt                       <  2:153476/250‑1 (MQ=255)

                                                                                                                                                                                                                             gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCt                                        <  1:69857/230‑1 (MQ=255)

                                                                                                                                                                                                                             gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCt                                        >  2:69857/1‑230 (MQ=255)

                                                                                                                                                                                                                                   cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt             >  1:181869/1‑251 (MQ=255)

                                                                                                                                                                                                                                   cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCCGCATCCGCGTGCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGTAAGAGATCCTCGGCGTCGAAGTTGACCAGTATGTGAAAGTTCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTg                <  2:339751/248‑1 (MQ=255)

                                                                                                                                                                                                                                      gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg          <  2:164632/251‑1 (MQ=255)

                                                                                                                                                                                                                                      gTCAGCGAACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg          >  1:273574/1‑251 (MQ=255)

                                                                                                                                                                                                                                         aGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGa        >  1:453286/1‑250 (MQ=255)

                                                                                                                                                                                                                                              aCCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGc  >  1:74726/1‑251 (MQ=255)

                                                                                                                                                                                                                                              aCCTGGGGCGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGAGTCCGGAATTTGc  >  2:30757/1‑251 (MQ=255)

AAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCAC‑AAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGC  >  NC_000913/23557‑24045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`