Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,297	C→T	L303L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,297	0	C	T	96.8%	239.7 / ‑6.0	95	L303L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (48/42);  minor base G (2/1);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

AAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTG‑AGCTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTT  >  NC_000913/23057‑23542                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCtt                                                                                                                                                                                                                                               >  1:459263/1‑251 (MQ=255) aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCtt                                                                                                                                                                                                                                               <  1:231411/251‑1 (MQ=255)    gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑c                                                                                                                                                                                                                                           >  1:638480/1‑251 (MQ=255)       aaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCTCCGGGCAGGCCGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTTACCGATTAAACCCTTCTCAACACGGTAAAATGAGCGGAGCTTG‑ATTTGCTGCGCTTTA‑ccc                                                                                                                                                                                                                                         >  2:669952/1‑250 (MQ=255)        aaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCAt                                                                                                                                                                                                                                       <  1:292442/251‑1 (MQ=255)             cccGTAAGCAACGTTAACGGCCCAATCTCGCTGGGGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCGGATTTCGCCTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCAGCCGtt                                                                                                                                                                                                                                  <  2:626852/250‑1 (MQ=255)                 aaaGCAACGTGAACGGCCCAATCTCGCTGGGGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAGCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATg                                                                                                                                                                                                                              <  2:176596/250‑1 (MQ=255)                         gTTAACGGCCCAATCCCGCTGGGGATCTGGACCACTACGCCGTTGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGa                                                                                                                                                                                                                      <  2:524652/251‑1 (MQ=255)                           tAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTGGCTGCGCTTTA‑CCCATCCGGTTAAGGGCTTCGACg                                                                                                                                                                                                                    >  2:137896/1‑251 (MQ=255)                              cGGCCCAATCTCGCTGGGTATCTGGACCCCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAGTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTc                                                                                                                                                                                                                 <  2:438627/251‑1 (MQ=255)                                      tctcGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGGTTATGGGCTTCGACGTTCCGGCAATc                                                                                                                                                                                                         >  1:275842/1‑251 (MQ=255)                                        tcGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCt                                                                                                                                                                                                       >  1:288800/1‑251 (MQ=255)                                                 aTCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATc                                                                                                                                                                                              >  1:334561/1‑250 (MQ=255)                                                 aTCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACAACATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCCTCCGTTTTTGGGCTTCGACGTTACGGCAATACTCgggcattc                                                                                                                                                                                              >  2:83258/1‑245 (MQ=255)                                                        ccactaCGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTAcc                                                                                                                                                                                      >  1:339763/1‑251 (MQ=255)                                                         cactacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCATTCCCCGGCA‑ATCACGTTAccc                                                                                                                                                                                     >  1:365022/5‑251 (MQ=255)                                                          actacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCt                                                                                                                                                                                    >  1:343709/4‑251 (MQ=255)                                                          actacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGGCCGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACAAAATTCTCAGCACGGGACAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCACTCCGTTTATGGGCCTCGACGTTCCGACAATACTCGGCG‑ATCACGTAACCCt                                                                                                                                                                                    >  2:624726/4‑251 (MQ=255)                                                                  gTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTTCACCATTCTCAGCACGGGTAAAGGTGCGGCGCTTG‑GGTTGCTGCGCTTTA‑CCCATCCGTTTCTGGGCTTAGTCGTTCCGGCAATCCTCGGCG‑ATCACGTTTCCCTggtttccg                                                                                                                                                                            >  2:286448/1‑245 (MQ=255)                                                                         ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTAcc                                                                                                                                                                       <  1:83258/249‑1 (MQ=255)                                                                         ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTAcc                                                                                                                                                                       <  2:104273/249‑1 (MQ=255)                                                                            cctgcgAACCGCGCTAACTTTATTGAGCCTGATTTCGACTACGCGCTGGTGCAGATCGACGGTCAGGGCGTGATTCTGGCGAAAGATCTGGTTTACAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGATCTTG‑AGTGGCTGCGCTTTC‑CCTGACCGTTTATTGGCGTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCttgacgtcggtctcggtgt                                                                                                                                                                  <  2:449555/251‑19 (MQ=255)                                                                             ctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGttt                                                                                                                                                                                                                                 <  1:455757/188‑1 (MQ=255)                                                                             ctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGAAAAAGGTGCGGGGCTTG‑GGTTGCTGCGCTTTA‑CCCATCCGttt                                                                                                                                                                                                                                 >  2:455757/1‑188 (MQ=255)                                                                                 gaaCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCAGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTc                                                                                                                                                             >  1:623105/2‑251 (MQ=255)                                                                                     cgcgAAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACAGATTACAACATTCTAAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CACATCCGTTTATGGGCTTCGACGATCCGGCAATACTCGGCG‑ATCACGTTACCCTGCATGACGGAAACGGTGCCGATCacac                                                                                                                                                         >  1:111556/1‑251 (MQ=255)                                                                                       cgcAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCAccc                                                                                                                                                       >  1:315340/1‑250 (MQ=255)                                                                                        gcAATCTCTATTGCACATGATTTCGACTATGCGCTGGCGCAGATCGACGGTCAGGCCGTGATTTTAGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATAACCACATTCACAGAACGGTAAAAGGCGCTGAGCTTGGATTTGC‑GCGCTTTAACCCCTC‑GCTTATTGGCTTCGACGTTACGGCAATCCACCGCGTACCA‑GTTACCCTGGATGCCGGTAccctgggcgttccacacac                                                                                                                                                      >  2:308210/1‑234 (MQ=255)                                                                                               ctATTGCACCTGATTTCGACGATGCGCTGGTGCAGATCGACGTGCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt                                                                                                                                                  <  2:340027/248‑1 (MQ=255)                                                                                                 atgtcACCTGATTCCGATTATGCGCCGGTGCAGATCGACGGTCAGACCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGTGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCTCTTTA‑CCCATCCGATTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCa                                                                                                                                             <  2:31043/247‑1 (MQ=255)                                                                                                            tttCGACTATGCGCTGGTGCAGATCGACGGTAATGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGCCCGATTACACCATTCTCAGCACGGTAAAAGGTACGGAGCTTG‑AGATGAGTCGCTTTA‑CCCCTCCGTTTAAGGTACTCGACGTTCCGGATATAACCGGCG‑ATAACGTAACCCTGTATGCCGGAGCAGGTGCCGTTCACACAGCGcccgcgcagcgcccggccg                                                                                                                                  >  2:129474/1‑234 (MQ=255)                                                                                                               cGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCAGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacgac                                                                                                                                <  2:623105/250‑1 (MQ=255)                                                                                                                     tgcgttgggtctgATCGACGGTCTGGCCGTGATTCTGGCGAAAGATCTGGTTGCAAGCGTTATGCTGATGATCGTCTTGACCGATAACACCATGCTCAGCACGGTAAAAGTTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCACCCGTGTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTCCCCTGGATGCCGGTTCCGGGGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtgtg                                                                                                                          <  2:510985/238‑1 (MQ=255)                                                                                                                      gcgcTGGGGCAGATCGACGGTCAGGCCGGAATTCTGGCGAAAGATCTGGTTGAAAGCGTAAGGCAGCGTATCGGCGTGACCGATTAAACCATTCTCATCACGGTAAAAGGGGCGGAGCTTG‑GGTTGCTGCGCTTTA‑ACCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGACG‑AACACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGAGACTAtgtgt                                                                                                                         >  1:228022/1‑249 (MQ=255)                                                                                                                       cgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATc                                                                                                                       >  1:445118/1‑251 (MQ=255)                                                                                                                        gcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGAGTGACCGAATACACCATTCTCAGCACGGGAAAAGGAGCGGAGCTTG‑AGATGCTGCGCTTTA‑ACCATACGTTTATGGGCTTCGACGTTACGGAAATCCTCGGGG‑AGCCCGTTACCCTGGATGCAGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGGCCGGCCGACTATGTGATCg                                                                                                                      >  1:445472/1‑251 (MQ=255)                                                                                                                         ggggTGCATATCGTCGTTCAGGCCGTGATTCTGGCGAAAGATGTGGTTGAAAGTGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCCCAGCACGGTAATAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGGCGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCTGGACGACTATGTTATCgg                                                                                                                     <  2:401310/249‑1 (MQ=255)                                                                                                                         cTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGGGATCgg                                                                                                                     >  1:537973/1‑251 (MQ=255)                                                                                                                         cTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGGTGCTGCGCTTTA‑CCCATCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCgg                                                                                                                     >  1:573958/1‑251 (MQ=255)                                                                                                                           ggTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTGCCCTGGATGCCTGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGt                                                                                                                    <  1:308210/250‑1 (MQ=255)                                                                                                                               cAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCCCGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTTATCGGTCAGa                                                                                                                >  1:274972/1‑250 (MQ=255)                                                                                                                                aGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGaaa                                                                                                              <  1:196756/251‑1 (MQ=255)                                                                                                                                       cGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGcc                                                                                                       <  1:541079/251‑1 (MQ=255)                                                                                                                                           catgacGTGTTTCTGACGAAAGATCTGGTGGAAAGCGTAATGCAGCGTATCGGCTGGACCTATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGTCGTTCCGGCAATCCGCGGCG‑ATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCttg                                                                                                    <  1:122374/245‑3 (MQ=255)                                                                                                                                           cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑TGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTgg                                                                                                    <  1:122376/250‑1 (MQ=255)                                                                                                                                           cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGCTCGGTCAGAAATACGGCCTggg                                                                                                   >  1:303345/1‑250 (MQ=255)                                                                                                                                             ggCCTTGATTCTGGCGAAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTTCACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑AGCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa                                                                                                 <  2:230020/251‑1 (MQ=255)                                                                                                                                               ccGTGAGTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAcc                                                                                               <  2:59892/251‑1 (MQ=255)                                                                                                                                                cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTGATCGGTCAGAAATACGGCCTGGAAAccc                                                                                              >  1:259523/1‑250 (MQ=255)                                                                                                                                                cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg                                                                                              >  1:264894/1‑251 (MQ=255)                                                                                                                                                 gTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGGTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCCCCTGGCCACGGCCCGGACGCCTATTGGAGCGGTCAGAAATACAGCCTGGCAAcagc                                                                                             >  2:131022/1‑248 (MQ=255)                                                                                                                                                  tGATTCTGGCGAAAGATCTGGTTGAAAGCGAAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCG‑ATCACGTTTACCTGGATGCCGGAACCGGTGCCGTTTACACCGCGCCTTGCCACCGCCCGGACGACCATGTGATCGGTCAGAAATACCGACAGGAAACCGcc                                                                                            >  2:521979/1‑250 (MQ=255)                                                                                                                                                         ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCgg                                                                                     <  1:283476/251‑1 (MQ=255)                                                                                                                                                            gcaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGGAAAAGGTGCGGGGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATTGGCGTCGCCGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCCGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGTTAGGGCAGAAAGACGGCCTGGACACCGCTACACCGGttt                                                                                  >  2:190066/3‑250 (MQ=255)                                                                                                                                                            gAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAACTACGGCCTGGGAACCGCTAACCCGGTTg                                                                                  >  1:73295/1‑251 (MQ=255)                                                                                                                                                            gAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTCAACGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTTCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAACGGGCAGGAATACGGCCTGGAAAcccataaacccgttg                                                                                  >  2:121969/1‑238 (MQ=255)                                                                                                                                                               aGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGcc                                                                               <  2:166281/251‑1 (MQ=255)                                                                                                                                                                   cTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTAATCGGGCAGAGATAAGGCCTGGGAAACGCTAACCCGGTTGGCCCggg                                                                           >  2:654273/1‑250 (MQ=255)                                                                                                                                                                     gggtGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTGCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCgg                                                                            <  2:303345/245‑1 (MQ=255)                                                                                                                                                                     ggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTCAAAGGTGCGGAGCTTG‑ATTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCGCGGACg                                                                         <  1:268204/251‑1 (MQ=255)                                                                                                                                                                         gAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTAAGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTAACACCGCGACTGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACCGCCCGGAAACCGCTAACCCGGTTGGCCCGGACGGCAc                                                                     >  1:446427/1‑251 (MQ=255)                                                                                                                                                                           aaGCGTAATGCAGCGTATCGGCGTGTCCGATTTCACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTG‑CTGTGCTGCGCTTTT‑ACCATCCGTTTATGTGCTTCGACGTTCCGTCAATCCACGGCG‑ATCCCGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTATCCCGGTTGGCCCGGTCGGCCCtt                                                                   <  2:488134/251‑1 (MQ=255)                                                                                                                                                                            aGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTa                                                                  <  1:669952/251‑1 (MQ=255)                                                                                                                                                                             gCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTAt                                                                 <  1:360699/251‑1 (MQ=255)                                                                                                                                                                              cGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCCCCGCCCGGACGGCTATGTGATCGGGCAGAAATAAGGCCTGGAACCCGCTAACCCGGTTGGCCCGGCAGGCACTTATc                                                                >  1:413560/1‑251 (MQ=255)                                                                                                                                                                               gTAATGCTGCGTATCGGCGGGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCGGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATa                                                                                                            <  2:269302/206‑1 (MQ=255)                                                                                                                                                                               gTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATa                                                                                                            >  1:269302/1‑206 (MQ=255)                                                                                                                                                                                      aGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACGGCCTGGAAAACGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGc                                                        >  1:658058/1‑251 (MQ=255)                                                                                                                                                                                         gTATCGGCGTGACCGATTACCCCATTCTCAGCACGGGAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACt                                                     <  2:138441/251‑1 (MQ=255)                                                                                                                                                                                         gTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACt                                                     <  1:233262/251‑1 (MQ=255)                                                                                                                                                                                             cGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTGG‑AGTTGCTGCGCTTTA‑CCCATCCGTGTATGGGCTTCGACGTACCGGCAATCCTCGGCG‑ATCCCGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTAt                                                  <  2:288808/250‑1 (MQ=255)                                                                                                                                                                                              ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGAGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTCACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                                                >  1:620648/1‑251 (MQ=255)                                                                                                                                                                                                cGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                                               >  1:675931/1‑250 (MQ=255)                                                                                                                                                                                                 gTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAc                                             <  2:334561/251‑1 (MQ=255)                                                                                                                                                                                                 gTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAAGGCCAGGAGACCGCTACCCCCGTTGGCCCGGACGGCACTTATCTGCCGGGCAATTATCCGAc                                             >  2:537484/1‑251 (MQ=255)                                                                                                                                                                                                   gACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑CGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCCAACCCGGTTGGCCCGGACGGAACTTATCTTCCGGGCACTTATCCgccc                                            >  2:569404/1‑247 (MQ=255)                                                                                                                                                                                                          tACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATg                                     <  2:343709/250‑1 (MQ=255)                                                                                                                                                                                                            cacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                  >  1:424719/1‑251 (MQ=255)                                                                                                                                                                                                            cacCATACTCAGCCCGGAAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGTCCTGGAAACAGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGCCGCTGGCTGGCg                                  >  2:293062/1‑251 (MQ=255)                                                                                                                                                                                                              ccATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCCCTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg                                >  2:341036/1‑251 (MQ=255)                                                                                                                                                                                                                   ctcagCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGt                           >  1:476600/5‑251 (MQ=255)                                                                                                                                                                                                                         aCGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCa                      >  1:276084/1‑250 (MQ=255)                                                                                                                                                                                                                          cGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCaaa                    <  1:479274/251‑1 (MQ=255)                                                                                                                                                                                                                              aaaaGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGaa                              >  1:134477/1‑237 (MQ=255)                                                                                                                                                                                                                              aaaaGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGaa                              <  2:134477/237‑1 (MQ=255)                                                                                                                                                                                                                              aaaaGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCg                 <  1:569404/250‑1 (MQ=255)                                                                                                                                                                                                                               aaaGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACgg                                                                                                         >  1:541393/1‑161 (MQ=255)                                                                                                                                                                                                                               aaaGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTAGGGGCTTCGACCTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTGATCGTTCAGAAATACgg                                                                                                         <  2:541393/161‑1 (MQ=255)                                                                                                                                                                                                                                taggagaggAGATTG‑AGGTGCTGCGCTTTA‑ACCATCCGTTTATGGGCTTCGACGTTCAGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGACTGGCCACGGACCGGACGACTATGTGAGCGGTCAGAACTACGGCATGGAAACCGCAACCCCGGATGGCCAGGACGGCAAGTATATGCCGGACACTGATCCGCCGCTGGATGGCGGGAACGCGTTCaaacgg                 >  2:54118/8‑245 (MQ=255)                                                                                                                                                                                                                                     gCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACa          <  1:137896/250‑1 (MQ=255)                                                                                                                                                                                                                                      cGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACACTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGGTGGCCCGGACGGCCCTTATCTGCCGGGCCCTTATCCGACGCTGGATGCCTGGAACGTCTTCCCAGCGAACGACAtc        >  2:324750/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggAGCTTG‑CGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCCATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCCAACCCGGTTGGCCCGGCCGGCACTTATCTGCCGGGCACTTATCCGACGCTGGTGGGCGTGAACGTCTTCAAAGCGAACGACAtcg       >  2:302240/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCTTG‑AGTTGCTGCGCTTGA‑CCCATCCGTTTATGGGCGTCGACGTTCCGGCACTCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCTGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcgtcg    <  2:445118/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCTTG‑AGTGGCTGCGCTGTA‑CCCATCCGTTTATGGGCTTCTACGTTCCGGCAATCCTCGGCG‑ATCCCGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcgtcg    <  2:275842/251‑1 (MQ=255)                                                                                                                                                                                                                                             tG‑AGTTGCTGAGCTGTT‑CCCATCCTGTTATGGGCTTCGACGTGCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCTACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAATCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGtt  <  1:115293/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        AAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTG‑AGCTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTT  >  NC_000913/23057‑23542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑